An 18-year-old female was admitted because of dyspnea at rest. A chest computed tomography (CT) scan and fiberoptic bronchoscopy demonstrated a polypoid tumor in the left main bronchus, 0.5cm distal from the carina. Surgical resection of the tumor was performed, along with. A pathological evaluation and the immunohistochemical findings led to the diagnosis of a glomus tumor, which originated from the bronchus, an area where this type of tumor has rarely been reported.
Adolescent ; Bronchi ; Bronchoscopy ; Diagnosis ; Dyspnea ; Female ; Glomus Tumor* ; Humans ; Thorax

BACKGROUND: Apoptosis has been implicated in pathogenesis of various disease. Apo-1/Fas (CD95) is one of the main pathway of apoptosis. To examine the possible relationship between Apo-1/Fas (CD95) and primary knee osteoarthritis, MvaI restriction length polymorphism (RFLP) in human Apo-1/Fas (CD95) gene was assessed. METHODS: Genotype and allele frequencies in promoter region in the Apo-1/Fas (CD95) gene were studied by PCR-RFLP in 226 Korean controls and 148 Korean patients with primary knee osteoarthritis. RESULTS: No statistically significant difference in the genotypic distribution and allelic frequencies was found between the control and the knee oateoarthritis patients. But in the severe grade (grade 3, 4) Kellgren-Lawrence score patients, the frequency of MvaI*1 (G) allele was significantly decreased (P=0.0392) and the of MvaI*2 (A) allele frequency was significantly increased (P=0.0473) compared to the normal controls. CONCLUSION: Apo-1/Fas (CD95) gene polymorphism is a part a determinant factor of severity in knee osteoarthritis, the patients with MvaI*2 (A) allele is more severe radiologic progression. Further substantiation studies are needed in larger patient samples and various other apoptosis related genes to elucidate the mechanism of osteoarthritis, including the Fas ligand gene analysis.
Alleles ; Apoptosis ; Fas Ligand Protein ; Gene Frequency ; Genotype ; Humans ; Knee* ; Osteoarthritis ; Osteoarthritis, Knee* ; Promoter Regions, Genetic

This study was conducted to estimate the prevalence rates and to explore associated factors of sarcopenic obesity (SO) in 2,221 Koreans over 60 yr-of age from the Fourth Korea National Health and Nutrition Examination Survey (2009). Participants were assessed by dual energy X-ray absorptiometry. Appendicular skeletal muscle mass divided by body weight was used to define sarcopenia and waist circumference was used to define obesity. We estimated the prevalence rates of SO according to age-groups, sex and region. In addition, each group was compared by demographic characteristics, metabolic status, nutrition, and physical activity. The prevalence rates of SO were 6.1% (95% confidential interval [CI] = 6.1-6.2) for men and 7.3% (95% CI = 7.3-7.3) for women, respectively. SO was positively associated with no current working and the number of combined medical conditions. High serum insulin level was positively associated with SO, whereas vitamin D was negatively associated with SO in both men and women. In conclusion, the prevalence rates of SO are 6.1% in men and 7.3% in women. SO is associated with insulin resistance, inappropriate nutrition, and low physical activity.
Age Factors ; Aged ; Aged, 80 and over ; Databases, Factual ; Demography ; Female ; Humans ; Insulin/blood ; Male ; Middle Aged ; Nutritional Status ; Obesity/*epidemiology/etiology ; Odds Ratio ; Prevalence ; Republic of Korea/epidemiology ; Risk Factors ; Sarcopenia/*complications ; Sex Factors ; Vitamin D/blood ; Waist Circumference

No abstract available.
Dyspnea* ; Humans

Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases, such as septicemia, pneumonia, urinary tract infection, and soft tissue infection. Klebsiella oxytoca is rarely isolated from human clinical specimens, and bacteremia due to Klebsiella oxytoca remains relatively uncommon. We experienced a case of liver abscess due to Klebsiella oxytoca in a 71-year-old male with a history of pylorus-preserving pancreaticoduodenectomy (PPPD) 4 years earlier. The patient was diagnosed with hemobilia secondary to a pseudo-aneurysm at the lower pole of the abscess cavity during antibiotic treatment and was treated successfully with selective percutaneous transcatheter embolization
Abscess ; Aged ; Bacteremia ; Hemobilia ; Humans ; Klebsiella ; Klebsiella oxytoca ; Liver ; Liver Abscess ; Male ; Pancreaticoduodenectomy ; Pneumonia ; Sepsis ; Soft Tissue Infections ; Urinary Tract Infections

Neural tissue is arisen from presumptive ectoderm via inhibition of bone morphogenetic protein (BMP) signaling during Xenopus early development. Previous studies demonstrate that ectopic expression of dominant negative BMP4 receptor (DNBR) produces neural tissue in animal cap explants (AC) and also increases the expression level of various genes involved in neurogenesis. To investigate detail mechanism of neurogenesis in transcriptional level, we analyzed RNAs increased by DNBR using total RNA sequencing analysis and identified several candidate genes. Among them, xCITED2 (Xenopus CBP/p300-interacting transcription activator) was induced 4.6 fold by DNBR and preferentially expressed in neural tissues at tadpole stage. Ectopic expression of xCITED2 induced anterior neural genes without mesoderm induction and reduced BMP downstream genes, an eye specific marker and posterior neural marker. Taken together, these results suggest that xCITED2 may have a role in the differentiation of anterior neural tissue during Xenopus early development.
Animals ; Bone Morphogenetic Proteins ; Ectoderm ; Embryonic Structures ; Eye ; Larva ; Mesoderm ; Neurogenesis ; RNA ; Sequence Analysis, RNA ; Xenopus

The epidermis is a physiological barrier to protect organisms against environment. During the aging process, skin tissues undergo various changes including morphological and functional changes. The transcriptional regulation of genes is part of cellular reaction of aging process. In order to examine the changes of gene expression during the aging process, we used the primary cell culture system of human keratinocytes. Since UV radiation is the most important environmental skin aggressor, causing skin cancer and other problems including premature skin aging, we examined the changes of gene expression in human keratinocytes after UV irradiation using oligonucleotide microarray containing over 10,000 genes. We also compared the gene expression patterns of the senescent and UV treated cells. Expression of the variety of genes related to transcription factors, cell cycle regulation, immune response was altered in human keratinocytes. Some of down-regulated genes are represented in both senescent and UV treated cells. The results may provide a new view of gene expression following UVB exposure and aging process in human keratinocytes.
Aging* ; Cell Cycle ; Epidermis ; Gene Expression* ; Humans* ; Keratinocytes* ; Oligonucleotide Array Sequence Analysis ; Primary Cell Culture ; Skin ; Skin Aging ; Skin Neoplasms ; Transcription Factors

Clinical features of facial skin cancer in Asian population including Korean are not readily available. In the present study, we analyzed the clinical characteristics and the surgical results of primary facial skin cancer in Chungbuk Province, Korea. Eighty-six cases of primary facial skin cancer collected during a 10-yr period (1994-2003) were retrospectively reviewed about the clinical characteristics including age, sex, annual diagnostic rate, types of tumor, specific sites of occurrence, and the surgical results. The average age at the diagnosis was 67 and male to female ratio was 1 to 1.05. The average annual diagnostic rate was 0.73% and the rate surged during the period 2001-2003 compared with the period 1994 to 2000. Basal cell carcinoma was the most common tumor and the nose was the most frequent site. Traditional surgical excision with immediate reconstruction was performed in 81 cases. During the 23 months of average follow-up, three patients had recurrences (3.7%) and three patients had secondary skin cancers. Facial skin cancer is increasing in the province and basal cell carcinoma is most frequent. Traditional surgical excision and immediate reconstruction with local flap are a good therapeutic modality with an acceptable recurrence rate.
Adult ; Aged ; Aged, 80 and over ; Carcinoma, Basal Cell/surgery ; Carcinoma, Squamous Cell/surgery ; Facial Neoplasms/*surgery ; Female ; Humans ; Male ; Middle Aged ; Research Support, Non-U.S. Gov't ; Skin Neoplasms/*surgery ; Treatment Outcome

BACKGROUND: Cord blood(CB) has been suggested as an alternate source of stem cells in transplantation because of lower incidence of graft versus host disease and ease of collection. To optimize storage space cord blood needs to be stored as separated product. Additionally, for cord blood bank to be economical and efficient, volumes smaller than that of whole packs needs to be stored. However, CB processing procedures described so far involve open systems and/or reagents which are not licensed for use in humans. Consequently, these procedures poorly match the needs for closed-system, large-scale CB banking. In our study, we use the triple bag system in which the CB is separated by centrifugation to maintain of sterility and volume reduction. And simple and effective RBC depletion method was evaluated with 10% pentastarch and 4% modified gelatin. METHODS: The collection of CB was performed from 45 full term newborns. The collection was made by gravity and uterine contraction before placental expulsion with triple transfusion bag containing CPD-A1. 10% Pentastarch(Group A) or 4% modified gelatin(Group B) is added directly to the CB containing bag. After RBC sedimentation, the WBC-rich supernatant is collected in a satellite bag. After RBC depletion, recovery of mononuclear cells(MNC), CD34+ cells were compared between two groups. RESULTS: Mean volume of collected CB and final RBC-depleted products were 68.1+/-17.0 mL and 18.1+/-4.1 mL in Group A and 68.0+/-15.8 mL, 23.1+/-6.3 mL in Group B, respectively. After RBC depletion, the number of MNC and CD 34+ cells were 2.61+/-1.51x108, 1.19+/-0.96x106 in Group A and 4.03+/-3.16x108, 1.43+/-0.86x106 in Group B. Mean recovery of MNC, CD34+ cells were 70.2+/-43.7%, 60.2+/-31.5% in Group A, and 84.7+/-39.9% and 87.7+/-7.5% in Group B, respectively. CONCLUSION: CB processing in closed-system using triple bag with 4% modified gelatin appears to be safe, easy, effective and particularly suitable for large-scale CB banking.
Centrifugation ; Fetal Blood* ; Gelatin ; Graft vs Host Disease ; Gravitation ; Humans* ; Hydroxyethyl Starch Derivatives ; Incidence ; Indicators and Reagents ; Infant, Newborn ; Infertility ; Stem Cells ; Umbilical Cord* ; Uterine Contraction

We treated 3 cases of fracture and 1 case of avascular necrosis of femoral head using autologous cultured osteoblasts injection. The stromal cells from the bone marrow were cultured to differentiate to osteoblasts for 4 weeks. The fracture sites of each patients were right ulnar shaft, left radial shaft, and left 5th metatarsal base. All of the fractures showed callus formations after 1 week of osteoblasts injection to the fracture site. After 4 weeks, callus formations were progressed. Avascular necrosis of femoral head was bilateral and both were Ficat stage II. Core decompression and allograft impaction were performed to the left, and core decompression and autologous cultured osteoblasts injection percutaneously after 4 weeks of the decompression operation were done to the right femoral head. CT images of 1 year from the operations showed trabecular bone formation and well maintained femoral head contour of the right femur, but resorption of the grafted bone for the left.
Allografts ; Bone Marrow ; Bony Callus ; Decompression ; Femur ; Head ; Humans ; Metatarsal Bones ; Necrosis ; Osteoblasts* ; Osteogenesis ; Stromal Cells ; Transplants