In spite of the extensive use of antibiotics over several decades, Streptococcus pneumoniae still remains as one of the most serious human bacterial pathogens. In order to clone the pneumococcal genes whose expression is induced when pneumococcus causes infection in mice, S. pneumoniae strain ATCC 6303 was subcultured on blood agar plates (BAP) ten times to reduce the virulence first, and then passaged through BALB/c mice three times to restore the virulence. Subtractive hybridization was performed using the total RNA preparations isolated from BAP-cultured and mouse-passaged strains. Complementary DNAs corresponding to any mRNA species that were differentially expressed in the mouse- passaged strain were used as the probes to screen the genomic library of pneumococcus. Positive recombinants were selected and sequenced partially to identify the genes located within the cloned DNA. GenBank search of the sequence data has identified several genes including two heat shock genes (dnaK and dnap, a transposase-encoding gene, and a sequence which is very homologous to that of the ftsH gene.
Agar ; Animals ; Anti-Bacterial Agents ; Clone Cells* ; Cloning, Organism* ; Databases, Nucleic Acid ; DNA ; DNA, Complementary ; Genomic Library ; Hot Temperature ; Humans ; Mice ; Pneumonia ; RNA ; RNA, Messenger ; Shock ; Streptococcus pneumoniae* ; Streptococcus* ; Virulence

Sparganum is parasite infesting principally in the cats or dogs, but many human reported. In Korea, the reported cases of human sparganosis are over 20 cases. However the urogenital infestation of sparganum is relatively rare, and none of any report of infestation in the epididymis is available. We experienced a case of the sparganum infesting on the epididymis which vas confused with the epididymitis on palpation.
Animals ; Cats ; Dogs ; Epididymis* ; Epididymitis ; Humans* ; Korea ; Male ; Palpation ; Parasites ; Sparganosis* ; Sparganum

No abstract available.
Dental Implants* ; Tissue Engineering*

No abstract available.
Adenocarcinoma* ; Colon* ; DNA* ; Flow Cytometry* ; Ploidies*

No abstract available.
Appendicitis* ; Child* ; Humans

No abstract available.
Hemoperitoneum*

PURPOSE: To evaluate the role of antegrade balloon dilatation and uteteral stenting in benign ureteral strictures. METHODS AND MATERIALS: Percutaneous antegrade balloon dilatation was attempted in 46 patients with benign ureteral strictures. The underlying causes of the strictures were urinary tract tuberculosis in 20 patients, congenital ureteropelvic junction obstruction in eight, ureteroneocystostomy or ureteroileostomy state in five, postoperative or post-extracorporeal shock wave lithotripsy state for ureteral calculi in eight, ureteral injury during surgery in four, and retroperitoneal fibrosis in one. RESULTS: Antegrade balloon dilatation was performed with initial technical success in 43 patients but the procedure was aborted in the remaining three with urinary tract tuberculosis due to the failure in passing a guidewire through the stenotic lesions. Intravenous urograms obtained 4-76 months after the procedure showed improvements in 76%(13/17) with urinary tract tuberculosis, in 63%(5/8) with congenital ureteropelvic junction obstruction, in 88%(7/8) with strictures associated with ureteral calculi, and in 100%(4/4) with iatrogenic ureteral injury. The results were relatively poor in strictures of the ureteral anastomosis(1/5) and in ureteralstrictures associated with retroperitoneal fibrosis(0/1). CONCLUSION: Antegrade balloon dilatation of the urinary tract combined with ureteral stenting was an effective technique for the management of the benign ureteral strictures.
Constriction, Pathologic* ; Dilatation* ; Humans ; Lithotripsy ; Retroperitoneal Fibrosis ; Shock ; Stents* ; Tuberculosis ; Ureter* ; Ureteral Calculi ; Urinary Tract

To assess the value of CT and MR in the detection of local tumor recurrence after hysterectomy, we evaluate CT and MRI findings of the vaginal stump in fifteen patients who underwent hysterectomy for uterine cervical carcinoma (N=14) or endometrial carcinoma (N=1). The presence of stump recurrence was confirmed by needle biopsy in 7 patients and by reoperation in 1 patient. The absence of stump recurrence was diagnosed by needle biopsy in 3 patitients and by clinical follow-up in 4 patients. In eight patients with vaginal stump recurrence, six patients were correctly diagnosed by CT and seven patients by MRI. In remaining seven patients who had no evidence of tumor recurrence, four patients were correctly diagnosed with CT and all the rester patients with MRI. ON the bases of our results, MRI seems to be superior to CT in the evaluation of local tumor recurrence after hysterectomy.
Biopsy, Needle ; Endometrial Neoplasms ; Female ; Follow-Up Studies ; Humans ; Hysterectomy* ; Magnetic Resonance Imaging* ; Recurrence* ; Reoperation

To assess the value of CT and MR in the detection of local tumor recurrence after hysterectomy, we evaluate CT and MRI findings of the vaginal stump in fifteen patients who underwent hysterectomy for uterine cervical carcinoma (N=14) or endometrial carcinoma (N=1). The presence of stump recurrence was confirmed by needle biopsy in 7 patients and by reoperation in 1 patient. The absence of stump recurrence was diagnosed by needle biopsy in 3 patitients and by clinical follow-up in 4 patients. In eight patients with vaginal stump recurrence, six patients were correctly diagnosed by CT and seven patients by MRI. In remaining seven patients who had no evidence of tumor recurrence, four patients were correctly diagnosed with CT and all the rester patients with MRI. ON the bases of our results, MRI seems to be superior to CT in the evaluation of local tumor recurrence after hysterectomy.
Biopsy, Needle ; Endometrial Neoplasms ; Female ; Follow-Up Studies ; Humans ; Hysterectomy* ; Magnetic Resonance Imaging* ; Recurrence* ; Reoperation

A statistical observation ryas made on Genito-Urinary-Tract injuries of Korean Army Personnel in Vietnam War during the period of November, 1965 to November, 1966. and the following results were obtained. 1. The Genito-Urinary-Tract injuries were 15 cases(5.3 per cent) among 825 war causalities. 2. As for the causes of injuries, 25 cases (55.5 per cent) were caused by gunshot, 9(20.O per cent) by booby trap, 5(11. O per cent) by mortar, 3(6.6 per cent) each by crush and grenade. 3. Concerning the frequency of injuries in each organ, 12 cases (6. 6 per cent) each were observed in urethra, in scrotum and testis, 10 (22.2 per cent) in kidney, 8 (17.7 per cent) in bladder, and 3 {6. 6 per cent) in penis. 4.The incidence of Genito-Urinary-Tract injuries associated with the other organs was as follows. In kidney injuries, Z cases were associated with intestine, 4 with liver, 3 with spleen and one each with radial nerve and iliac vessel. In bladder injuries. 2 cases were associated with pelvic bone, and one with spleen. In urethra injuries, 6 cases were associated with pelvic bone and 2 with intestine. 5. As for the multiplicity of Genito-Urinary-injuries, 4 cases were observed in scrotum, testis and urethra, and 3 each in urethra and bladder, and scrotum and testis. 6. The evacuation time from battle fields to the evacuation hospital was one hour in average by helicopter
Aircraft ; Humans ; Incidence ; Intestines ; Kidney ; Liver ; Male ; Military Personnel ; Pelvic Bones ; Penis ; Radial Nerve ; Scrotum ; Spleen ; Testis ; Urethra ; Urinary Bladder ; Vietnam*