1.Invasiveness and Proliferative Activity of Oral Squamous Cell Carcinoma : Immunohistochemical Study Using Laminin, Type IV Collagen, and Ki-67 Antibody.
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1997;23(3):401-417
Since the invasiveness and metastatic potential of the carcinoma and the proliferation of the tumor cells are the decisional factors in determining the prognosis of carcinoma, it has been a principal area of study to foresee these factors in early stage of the disease process. The purpose of the study was to propose a method to precisely determine the prognosis of tumor. The investigation was performed, using laminin, type IV collagen, and Ki-67 antibody, to reveal the invasiveness and proliferation of the squamous cell carcinoma which is the most prevalent cancer in the area of oral and maxillofacial complex. Frozen tissue sections, 6-8mum in thickness, were prepared from the surgical excision of fresh squamous cell carcinoma for the experimental group. The control group was composed of sections from the normal oral mucosa of the contralateral side. Following incubation in an appropriate primary antibody including laminin, type IV collagen and Ki-67, LSAB method was employed and the tissue sections were treated with DAB for light microscopic immurlohistochemistry. Semiquantitative analysis of the basement membrane was composed of five different positivity of laminin and type IV collagen according to the intensity of the staining. Mean number of Ki-67 positive cells per 2000 total cells in one slide were calculated along with the standard deviation. The obtained results were as follows : 1. In the basement membrane of normal oral mucosa, antibodies against laminin and type IV collagen showed regular thickened, continuous line. Positive reaction was also detected in the vascular walls. Ki-67 labelling index was 4.10+/- 0.80%, and the positive cells were distributed mainly in the basal and parabasal cell layers. 2. In basement membrane of the proliferating tumor adjacent to carcinoma, antibodies against laminin and type IV collagen showed irregular thin, discontinuous line. Ki-67 labelling index was 18.95+/-4.67%, and the parabasal cell layer exhibited increased number of positive cells. 3. In the basement membrane of the main proliferating tumor, antibodies against laminin and type IV collagen showed almost indistinct interrupted line and/or almost indistinct line. Ki-67 labelling index was 31.56+/-5.51%, and the parabasal and spinous cell layer indicated increased cell positivity. 4. Invasive islands showed almost negative reaction to the antibody against type IV collagen, and almost indistinct line of the laminin antibody was observed. Based upon these findings, the loss of laminin and type IV collagen and the Ki-67 labelling index were the highest in the basement membrane of invasive islands. Therefore, it is thought that invasive islands were more deeply involved, than main proliferating tumor, in the invasiveness and proliferation of tumor, and the more the invasiveness of the tumor, the more the cell proliferative activity.
Antibodies
;
Basement Membrane
;
Carcinoma, Squamous Cell*
;
Collagen Type IV*
;
Islands
;
Laminin*
;
Mouth Mucosa
;
Prognosis
2.Study of Skin Disorders in Alcoholic Patients.
Korean Journal of Dermatology 1990;28(5):575-581
No abstract available.
Alcoholics*
;
Humans
;
Skin*
3.Excessive Daytime Sleepiness Case Confounding with Thyrotoxicosis.
Jae Kyung CHUNG ; Eui Joong KIM
Sleep Medicine and Psychophysiology 2011;18(1):40-44
Narcolepsy is a sleep disorder, which is characterized by excessive daytime sleepiness (EDS) that is typically associated with cataplexy, sleep fragmentation and other REM sleep-related phenomenon such as sleep paralysis and hypnagogic hallucination. Narcoleptic symptoms can be developed from various medical or neurological disorders. A 17-year-old male patient admitted for the evaluation of EDS which started three-month ago. He slept more than 18 hours a day with cataplexy and hypnagogic hallucination. He was obese with body mass index (BMI) of 30.4 kg/m2. After admission he was newly diagnosed to the thyrotoxicosis. T3 391.2 ng/dL (60-181), free T4 4.38 ng/dL (0.89-1.76), TSH <0.01 microIU/mL (0.35-5.5) were measured. His pulse rate ranged 70-90 beats per minute and blood pressure ranged 150/100-120/70 mmHg. Polysomnography revealed many fragmentations in sleep with many positional changes (81 times/h). Sleep onset latency was 33.5 min, sleep efficiency was 47.9%, and REM latency from sleep onset was delayed to 153.6 min. REM sleep percent was increased to 27.1%. Periodic limb movement index was 13.4/h. In the multiple sleep latency test (MSLT), average sleep latency was 0.4 min and there were noted 3 SOREMPs (Sleep Onset REM sleep period) on 5 trials. We couldn't discriminate the obvious sleep-wake pattern in the actigraph and his HLA DQB1 *0602 type was negative. His thyroid function improved following treatment with methimazole and propranolol. Vital sign maintained within normal range. Cataplexy was controlled with venlafaxine 75 mg. Subjective night sleep continuity and PLMS were improved with clonazepam 0.5 mg, but the EDS were partially improved with modafinil 200-400 mg. Thyrotoxicosis might give confounding role when we were evaluating the EDS, though sleep fragmentation was one of the major symptoms of narcolepsy, but enormous amount of it made us think of the influence of thyroid hormone. The loss of sleep-wake cycle, limited improvement of EDS to the stimulant treatment, and the cataplexy not supported by HLA DQB1 *0602 should be answered further. We still should rule out idiopathic hypersomnia and measuring CSF hypocretin level would be helpful.
Adolescent
;
Benzhydryl Compounds
;
Blood Pressure
;
Body Mass Index
;
Cataplexy
;
Clonazepam
;
Cyclohexanols
;
Extremities
;
Hallucinations
;
Heart Rate
;
HLA-DQ beta-Chains
;
Humans
;
Hypersomnolence, Idiopathic
;
Intracellular Signaling Peptides and Proteins
;
Male
;
Methimazole
;
Narcolepsy
;
Nervous System Diseases
;
Neuropeptides
;
Polysomnography
;
Propranolol
;
Reference Values
;
Sleep Deprivation
;
Sleep Paralysis
;
Sleep, REM
;
Thyroid Gland
;
Thyrotoxicosis
;
Vital Signs
;
Orexins
;
Venlafaxine Hydrochloride
4.Clinical & arthroscopic findings of chronic anterior cruciate ligament injury.
Journal of the Korean Knee Society 1993;5(2):145-149
No abstract available.
Anterior Cruciate Ligament*
5.Vascular Pedicle Rib Graft for the Anterior Interbody Fusion of the Spine
The Journal of the Korean Orthopaedic Association 1985;20(1):25-31
No abstract available in English.
Ribs
;
Spine
;
Transplants
6.Correction: Characteristics of Pediatric Pancreatitis on Magnetic Resonance Cholangiopancreatography.
Jae Yeon HWANG ; Hye Kyung YOON ; Kyung Mo KIM
Pediatric Gastroenterology, Hepatology & Nutrition 2015;18(3):216-216
The errors were discovered after publication: missing references and missing words.
7.Clinical Statistics of Apgar Scoring System of Newborn.
Soo Kyung JEONG ; Jae Sook KIM ; Choong Hee KIM
Journal of the Korean Pediatric Society 1984;27(3):217-223
No abstract available.
Humans
;
Infant, Newborn*
8.A Case of Retinal Periphlebitis Associated With Barre Syndrome.
Sook Kyung CHOI ; Jae Ho KIM ; Sang Min KIM
Journal of the Korean Ophthalmological Society 1969;10(4):33-35
Authors' presented an interesting case, 19 years old boy, who was been suffered from retinal periphlebitis of both eyes which thought to be induced by Barre Syndrome. This patient also has pulmonary tuberculosis and visual symptoms of Barre Syndrome in this case were ocular pain and visual disturbance that appeared and disappeared suddenly due probably to irritation of the ventral nerve roots (C5 to T1) with also irritation of sympathetic fibers contained in them.
Adrenergic Fibers
;
Humans
;
Male
;
Phlebitis*
;
Retinaldehyde*
;
Tuberculosis, Pulmonary
;
Young Adult
9.Anatomical Study on the Location of the Mental Foramen in Adult Korean Mandibles.
Kyung Won YOON ; Kang Ryune KIM ; Jae Hyung WOO ; Jin Jeong KIM ; Jae Bong KIM
Korean Journal of Physical Anthropology 1989;2(1):11-17
We examined the anatomical position of the mental foramina in mandibles foramen normal adult Koreans. 1. The percentages obtained from the study of the relationships between the mental foramen and the lower teeth showed that the most common location was type lv in which the mental foramen lay at the apex of the second promolar. The foramen between thr apices of ice two premolars (type lll) and the foramen between the second premolar and the first molar (type v) occured often and less often rspectively and find no foramen mesial to the first premolar or at the apex of the first premolar and posterior of the first molar (type l, ll, vl). 2. The study of relationship of the mental foramen to the bo of the mandible revealed that mental foramen was situated closer to the lowed border of the mandibular body. The distance ratio between the mental foramen and the alveolar crest to that between the mental foramen and the lower border was approximately 1.2 : 1. The height of the mandibular body was 31.09±2.80mm on the left side and 30.97±2.48mm on the right. 3. The distance from the mandibular symphysis to the anterior border of the mental foramen measured 29.52±2.01mm on the left, 30.82±2.04mm on the right side, and from the mandibular symphysis to the posterior border of the mandibular ramus was 104.20±4.74mm on the left, 105.44±4.49mm on the right side. It indicates that the mental foramen lies approximately at one-fourth of the distance from the mandibular symphysis to 2017-04-19 the posterior border of the ramus. 4. The distance from the superior border of the mental foramen to the bottom of the lower second premolar socket was found to be positive. It was 5.46±3.09mm on the left, 5.73±3.03mm on the right side. This indicates that the bottom of the lower second premolar socket is slightly higher than the superior border of the mental foramen.
Adult*
;
Bicuspid
;
Humans
;
Ice
;
Mandible*
;
Molar
;
Tooth
10.Immune complex-induced increases in collagen production by cultured mesangial cells modulated by dexamthasone and heparin.
Chun Gyoo IHM ; Jae Kyung PARK ; Jae Hyung AHN ; Tae Won LEE ; Myung Jae KIM
Korean Journal of Nephrology 1992;11(3):207-213
No abstract available.
Collagen*
;
Heparin*
;
Mesangial Cells*