No abstract available.
Acidosis, Renal Tubular*

Cytochrome P-450 (CP-450) is one of the three components of the liver microsomal enzyme system which hydroxylates fatty acids, hydrocarbons and a variety of drugs and other foreign compounds. Female Balb/c mice were immunized with purified polychlorinated biphenyl (PCB) – induced CP-450 LMII. The spleen cells derived from immunized mice were fused with SP2 myeloma cells using polyethylene glycol (PEG 3500). The hybrid cells were selected by hypoxanthine-aminopterin-thymidine (HAT) medium and the culture fluid were screened by enzyme-linked immunosorbent assay to CP450 LMII. The hybrid cells(×107) were inoculated into intraperitoneal cavity of Balb/c mice for the purpose of production of ascetic fluids. Monoclonal antibody (Mab) was purified from ascitic fluid by DEAE cellulose ion exchange chromatography and I¹²⁵ labeled Mab was also confirmed by autoradiography and SDS-polyacrylamide gel electrophoresis (MW:55,000 and 110,000)
Animals ; Ascitic Fluid ; Autoradiography ; Chromatography, Ion Exchange ; Cytochrome P-450 Enzyme System* ; Cytochromes* ; DEAE-Cellulose ; Electrophoresis ; Enzyme-Linked Immunosorbent Assay ; Fatty Acids ; Female ; Humans ; Hybrid Cells ; Hydrocarbons ; Liver* ; Mice ; Polyethylene Glycols ; Rats* ; Spleen

Band 3, the predominant 95,000 dalton anion transport protein, is the major intrinsic glycoprotein of the human erythrocyte membrane. This anion carrier exists as a dimer and binds the cytoskeletons such as spectrin, ankyrin and actin. And the liposomes are vesicular structures which form spontaneously upon hydration of phospholipids. These artificial lipid vesicles have been investigated as model of the biological membranes and as a mean of improving the delivery of nucleic acids, drugs, proteins and biological substances to specific target tissues and cells. In this study, we were purified Band 3 from the human erythrocyte membrane (ghost) was prepared by hemolysis of intact human erythrocyte with weak alkali-hypotonic solution. Band 6 was removed from ghost by extracting with solution of an ionic strength of 0.15. Band 3 and Band 4 were solubilized selectively by extracting Band 6-depleted ghosts with Triton X-100 under nondenaturing conditions. Band 3 was then purified from Triton X-100 extract treated with p-chloromercuribenzoate by sucrose density gradient ultracentrifugation. This purified Band 3 was incorporated into liposomes prepared by reverse-phase evaporation. Phosphatidyl L-serine and cholesterol (1:1 molar ratio) were dissolved in chloroform and the chloroform was removed by rotator evaporation under reduced pressure. Band 3 solution without Triton X-100 was introduced into a mixture of lipids and diethylether. Diethylether was subsequently removed by evaporation. This purified Band 3 and its incorporation into liposomes were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Actins ; Ankyrins ; Chloroform ; Cholesterol ; Cytoskeleton ; Electrophoresis ; Erythrocyte Membrane* ; Erythrocytes* ; Glycoproteins ; Hemolysis ; Humans* ; Liposomes* ; Membranes ; Molar ; Nucleic Acids ; Octoxynol ; Osmolar Concentration ; Phospholipids ; Serine ; Sodium ; Spectrin ; Sucrose ; Ultracentrifugation

A 33 years old house wife with accessory breast tissue is described and the literature is reviewed. The patient developed soft tumors in both axillae with axillary hair loss during the 8 th month of her 8 th pregnancy. This disorder is rather common in the literature but has been paied little attention dermatology.
Adult ; Axilla ; Breast* ; Dermatology ; Hair ; Humans ; Pregnancy ; Spouses

OBJECTIVES: This study evaluates the change of the left atrial appendage function before and after electrical cardioversion to understand the mechanism involved in systemic thromboembolism of atrial fibrillation. BACKGROUND: Systemic thromboembolism associated with electrical cardioversion of atrial fibrillation is thought to originate from the left atrium or left atrial appendage, or both.However, the mechanism involved is poorly understood. METHOD: We studied left atrial appendage function funcction with transesophageal echocardiography in 15 patients with atrial fibrillation before and after successful electrical cardioversion. We measured left atrial appendage emptying and filling velocities and left atrial appendage areas. Also we analysed the characteristic Dopper flow pattern of LAA. RESULT: Left atrial appendage emptying velocities before cardioversion were greater in patients without(32.0+/-13.2cm/sec) than in those with(21.4+/-7.6cm/sec) spontaneous echo contrast(SEC). Furthermore emptying velocities after cardioversion were significantly reduced group with (21.4+/-7.6 vs 12.2+/-9.6, p<0.05) and the groupwithout(32.0+/-13.2 vs 18.1+/-10.2, p<0.05)SEC. CONCLUSION: After electrical cardioversion for atrial fibrillation left atrial appendage function is impaired. These observations suggest that stunned left atrial appendage after cardioversion may predispose to thrombus formation, which may play a role in the mechanism involved in the occurrence of thromboembolism after cardioversion.
Atrial Appendage* ; Atrial Fibrillation* ; Echocardiography, Transesophageal* ; Electric Countershock* ; Heart Atria ; Humans ; Thromboembolism ; Thrombosis

From September 1983 to August 1984, the clinical observations regarding cutaneous manifestations of 26(j cases of in-patients in the Department of Internal medicine with hepatic problems were observed at the Department of Derrnatology in Chonnam University Eiospital, The results were as follows: In the 266 patients, 201 patients(75.6%) revealed various cutaneous manifestaiions; Among them vascular spider occured in 100 (37. 6%), bleeding tendency in 67(25. 2%), pruritus in 65(24.4%), jaundice in 59 (22.2%), palmar erythema in 59(22. 2%), and melanosis in 56(21. l%). The incidences of skin changes by pattern of liver disease were as follows; In acte hepatits, prurit;us(46.8%) and acute urticarial & maculapapular eruption(36. 4%) were found in order. In chronic hepatitis, vascular spider(42. 6%) and melanosis (27. 9%) in order. In cirrhosis, vascular spider (62. 5%) and bleeding tendency (42. 0%) in order. In hepatic cancer vascular spider (24. 2%) and jaundice (21. 2%) in order. There were no statistically significant differences in sex distribution. In the incidences of skin changes by age groups, jaundice(p<0. 01) and melanosis were more frequently observed in the patients under the age of 29 and vascular spider (p<0.01 ) and melanosis in the patients are the age of 50. The longer the duration of illness were, the higher the incidences of skin changes except jaundice were.
Erythema ; Fibrosis ; Hemorrhage ; Hepatitis, Chronic ; Humans ; Incidence ; Internal Medicine ; Jaundice ; Jeollanam-do ; Liver Diseases ; Liver Neoplasms ; Melanosis ; Pruritus ; Sex Distribution ; Skin Manifestations* ; Skin* ; Spiders

The subjects of the present study were 111 patients admitted to a hospital and 304 healthy people living in similar condition. A self report symptom inventory, SCL-90(symptom check list-90) was used and the two groups were analysed and compared by many factors. The results of theis study are as follows. 1. All dimensional values of the psoriasis group were much higher than the normal healthy group. In order of significance: depression, anxiety, obsessive-compulsive, psychotism, interpersonal sensitivity, hostility, and somatization(all p<0.01). This result indicates a significantly heightened incidence of psychological problems among psoriatic patients. 2. There were significant differences between each clinical type. As a whole, common and pustular types showed highter dimensional scores than guttate type. 3. In the psoriasis group, subjects over 30 years old had higher scores except in somatization and paranoid ideation dimensions. However there was no significant relationship between age and dimensional scores. 4. Female psoriatic patients showed higher values than male patients on all dimensions excopt paranoid ideation. T verification showed significance in depression and hostility (p<0.05). 5. Analysed by marital status, generally unmarried patients had higher dimensional scores than married patients, but there was no significant differences. 6. In contrast with the control group, psoriatic patients of low educational status showed higher dimensional scores than patients of high educational status. 7. Analysed by job at onset of disease, student patients showed the lowest scores. There were significant differences for somatization(p<0.01) and paranoid (p<0.05) dimensional scores. 8. Analysed by the age of onset of disease, the patients who developed psoriasis before 20 years of ages showed higher dimensional scores than the patients, after 20 years of age. 9. Compared according to duration of the disease, dimensional scores rose in patients under 1 year duration decreased in patients of 1 to 2 years' duration and rose again in parients over 3 years' duratio. 10. As the accuracy of understanding about the disease decreased, the dimensional scores tended to be higher.
Adult ; Age of Onset ; Anxiety ; Depression ; Educational Status ; Female ; Hostility ; Humans ; Incidence ; Male ; Marital Status ; Psoriasis ; Self Report ; Single Person

Two cases with generalized G.A. were described. The first case was 42 years, old farmer representing slightly erythematous multiple papulonodular lesions on the face, neck, scapular areas, arms and dorsal hands. Coalescence of lesions in some areas of the dorsal hands and neck produced circinate or polycyclic pattern. The second case was 4 years old girl who developed purple to brown muliple papulonodular lesions and polygonal or round plague on the arms, dorsal hands, palms, neck, iliac crests, lower legs, dorsal feet and sole. The plague lesions mimick lichen planus.
Arm ; Child, Preschool ; Female ; Foot ; Granuloma Annulare* ; Granuloma* ; Hand ; Humans ; Leg ; Lichen Planus ; Neck ; Plague

Epdemiological studies were done on 721 patients detected and confirmed as leprosy patients at the Department of Dermatology at Chonnam University Hospitai in the ten years period from l969 to 1978. Statistically, 491 cases were male and 230 cases were female, comprising 2. 1% of the total number of outpatients (34, 631) and showing a decreasing yearly tendency, The percentage of each group, or type; tuberculoid, borderline, lepromatous and indeterminate, was 53.8%, 9.6%, 31.2%, and 5.4%, respectively. The most common age at discovery for males was 30 39 and for female 20~29 with the largest group being adults aged 20~49, 482 cases (66.9%). The sex ratio was 2.1: l, male to female. However, for the tuberculoid types the ratio was 2.5: 1 which was higher than that of the lepromatous type, I. 7: 1, By disease type the most common age group was 20 29 for tubercekid type, 30 39 for lepromatous and borderline group and 10~19 for the indeterminated group. Geographically, 652 cases(90. 4%) were from Chonnam Province and 61 cases (8.5%) from Chonbuk Province. We also observed some significant sequential changes of the type of disease descovered even though 10 years is a relatively short period. There was a tendency towards decreased detection of the tuberculoid type and increased detection of the borderline an.d indeterminate group.
Adult ; Dermatology ; Female ; Humans ; Jeollabuk-do ; Jeollanam-do ; Leprosy* ; Leprosy, Paucibacillary ; Male ; Outpatients ; Sex Ratio

We studied skin thickness of the twelve body sites in 25 post-mortem subjects. The most thinnest and thickest sites of the skin layers are as follows(unit: um) l. In the horny layer, eyelid(13.16+/-5.44) and sole(420.83+182. 25), respectively 2. In the granular, eyelid, posterior ear, abdomen, back, thigh, forearrn(1 layer)and sole(3.4+/-0.59 layers), respectively 3. In the prickle cell layer(to rete ridge), forearm(50. 21+/-15. 36) and sole (174.72+/-84.41), respectively 4. In the prickle cell layer(to papillary dermis), abdomen (33. 05+/-10. 28) and sole (106.13+/-73.62), respectively 5. In the epidermis, eyelid (61.60+/-16.53) and sole (972.00+/-228. 16), rpspectively 6. In the dermis, eyelid(878.08+283. 56) and back(3596.05+/-1306.80), respectively.
Abdomen ; Dermis ; Ear ; Epidermis ; Eyelids ; Skin* ; Thigh