No abstract available.
Titanium*

Accurate reproduction of intraoral tissue is essential in the fabrication of fixed prosthesis. This selection of impression material and method is very important. In this study, vinyl polysiloxane type impression material Panasil was used. Three impression methods ; one-step technique, individual tray technique, and individual tooth tray technique using the vinyl polysiloxane type bite registration material Futar Occlusion, were used to take horizontal and vertical impression. Improved stone models were fabricated. The amount of occlusal surface discrepaneies, interabutment distance discrepancies, amount of marginal defect were evaluated with the X-PLAN360d and photoscanning. The results were as follows. 1. There was no significant difference in occlusal surface according to the directions and techniques of impression taking. 2. There was no significant difference in interabutment distance discrepancies according to the directions and techniques of impression taking 3. There was no significant difference in marginal discrepancies according to the direction of impression taking but there was significant difference between one-step technique and individual tray technique, individual tooth tray technique (P<0.05). Taken together, these results suggest that individual tray technique and individual tooth tray technique are more accurate for impression taking than one-step technique.
Jaw Relation Record ; Prostheses and Implants ; Reproduction ; Siloxanes ; Tooth

OBJECTIVE: Immunofluorescence microscopy including confocal laser scanning microscopy and electron microscopy were used to study the production of fibronectin, tenascin, and laminin in the cumulus-corona (CC) cells surrounding mature, unfertilized oocytes after ovulation in view of their presumptive importance in the coordination of the processes leading to fertilization and early embryo cleavage, including the final maturation of the ovum, the sperm-egg interaction, and the complex biochemical mechanism between the ovum and the oviduct. METHODS: Mature oocyte-cumulus complex (OCC) was cultured for 24 and 48 hour and fixed in 3.7% formaldehyde. Specimens were incubated with a mixture of primary monoclonal antibodies recognizing different epitopes of fibronectin, tenascin, and laminin, and then with a mixture of secondary antibodies containing FITC, TRITC, and Cy-5 conjugated antibodies. Observation was made by confocal laser scanning microscope equipped with epifluorescece optics. Transmission electron microscopy were used to observe the OCC at 24 and 48 hours after cultrue. RESULTS: The immunocytochemical date demonstrated that CC masses are capable of producing fibronectin and tenascin but their production is heterogeneous in the CC population. Immunoreactivity to fibronectin and tenascin was shown mostly by inner corona cells, and the intensity of immunofluorescence decreased from the central corona cells to the peripheral cumulus cells. Colocalization of fibronectin and tenascin was evident in most CC cells. Moreover, fibronectin and tenascin immunoreactive material was observed in the intracytoplasmic areas, at the plasma membrane level as well as in the extracellular matrix. Whereas, laminin immunofluorescence was found around plasma membrane and extracellular area, but a intracytoplasmic reaction was rarely observed. The distribution of laminin immunofluorescence was similar to that of fibronectin and tenascin, but in some cumulus cells, colocalization between them was not found. Ultrastructurally, cumulus cells projected numerous long, thin microvilli into the intercellular area and some micovilli penetrated into zona pellucida. The inner layer of the cumulus mass was loose arrangement of relatively uniform, small cells with widened intercellular spaces, whereas in the outer layer, cumulus cells are rather larger in size and compact arrangement by narrow, irregular spaces. A small and large linear gap junctions were easily found at cell contacts. The cytoplasm of most cells had abundant organelles typical of steroidogenesis: numerous mitochondrias, a well-developed smooth endoplasmic reticulum, electron dense lipid droplets, and bundles of microtubules and microfilaments. Rudimentary disrupted basal lamina along the cytoplasmic border was rarely seen in a few inner conora cells. CONCLUSION: Even though the functional role of these extracellular matrix proteins remains still unclear, it is reasonable to suggest that they are necessary in various steps of the reproductive process. Cumulus cells appears to be a heterogeneous and dynamic system for suitable microenviroment of fertilization. And functional differences between corona and cumulus cells during the oocyte denudation may be accounted for particular distribution of these adhesive proteins and steroidogenesis-related organelles.
Actin Cytoskeleton ; Adhesives ; Animals ; Antibodies ; Antibodies, Monoclonal ; Basement Membrane ; Cell Membrane ; Cumulus Cells ; Cytoplasm ; Embryonic Structures ; Endoplasmic Reticulum, Smooth ; Epitopes ; Extracellular Matrix ; Extracellular Matrix Proteins ; Extracellular Space ; Female ; Fertilization ; Fibronectins* ; Fluorescein-5-isothiocyanate ; Fluorescent Antibody Technique ; Formaldehyde ; Gap Junctions ; Immunohistochemistry ; Laminin* ; Microscopy, Confocal ; Microscopy, Electron* ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Microtubules ; Microvilli ; Mitochondria ; Oocytes ; Organelles ; Oviducts ; Ovulation ; Ovum ; Sperm-Ovum Interactions ; Tenascin* ; Zona Pellucida

Epidermal nevus syndrome is now delineated as a congenitally acquired syndrome consisting of deformities of the skin (various linear epidermal or organoid nevi), central nervous system, skeleton, cardiovascular system, and renal system. A 4-year-old girl had linear epidermal nevus on the right lower extremity, right ear lobe and anterior neck, and multiple skeletal abnorrnalities since birth. And she had an adult fist-sied, soft, nontender mass on the right lower abdomen since 2 years of age. Radiologic examination showed scoliosis of the thoracic spines, deformities of the lumbar spines, focal gigantisms of the left hand and right foot, and other skeletal abnormalities. Histological examination of the linear, verrucous lesion of the right leg showed hyperkeratosis and papillomatosis. The surgically removed abdominal mass revealed the finding of hydrosalpinx.
Abdomen ; Adult ; Cardiovascular System ; Central Nervous System ; Child, Preschool ; Congenital Abnormalities ; Ear ; Female ; Foot ; Hand ; Humans ; Leg ; Lower Extremity ; Neck ; Nevus* ; Organoids ; Papilloma ; Parturition ; Scoliosis ; Skeleton ; Skin ; Spine

In the case of a soft tissue defect which requires thin & pliable tissues, the choice of donor site is limited due to flap bulkiness and donor site morbidity. To overcome these problems, a variety of perforator-based flaps such as paraspinous and parasacral perforator flap, deep inferior epigastric perforator flap, gluteal perforator flap, and thoracodorsal artery perforator flap have recently been introduced. We experienced 8 cases of soft tissue defects from December 1996 to March 1999 using the thoracodorsal artery perforator flap for reconstruction. We could elevate the cutaneous flap with preservation of the latissimus dorsi muscle flap only when it was based on one cutaneous perforator. Defatting procedure was possible for further thinning of skin flap. In one case, axillary defect after release of postburn scar contracture was repaired with island perforator flap and the other seven cases were repaired with free flap. The results were satisfactory. We believe the thoracodorsal artery perforator flap is useful for reconstruction of soft tissue defects which are large or under conditions requiring thin flap.
Arteries* ; Cicatrix ; Contracture ; Free Tissue Flaps ; Humans ; Perforator Flap ; Skin* ; Superficial Back Muscles ; Tissue Donors

BACKGROUND: Although it is evident that androgens have an important role in pathogenesis of acne, the results of current studies on the relationship between serum androgens and acne are inconsistent. Additionally there has been sparse clinical datas about hormonal derangcment with regard to acne in Korea. OBJECTIVE: This study was conducted to elucidate hormonal abnormalities by detecting the quantitative difference between the acne patients and normal persons with respect to the levels of plasma free/total Testosterone(T), Dihydrotestosterone(DHT), Dehydroepiandrosterone-sulfate(DHEA-S) and Estradiol(E2) in acne patients. METHODS: In this study, we measured serum levels of free T, total T, DHT, DHEA-S and E2 in 25 women and in 15 men with acne and in 40 healthy control subjects. RESULTS: 1. The levels of free T and E2 in the male patient group were significantly higher than those in the control groups. In the female patient group the levels of DHT were more elevated than those in the control groups. 2. The levels of free T and E2 in all the male subgroups classified by Burke & Cunliffe were significantly elevated compared to those in the male control groups. The plasma levels of DHT in all the female clinical subgroups were significantly higher than those in the female control groups. However, we could not find any correlative relationship between hormonal parameters and the clinical severity in all the patient groups. 3. There was a positive relationship between free T levels and E2 levels in the male patient gfOUp. 4. The patients with menstrual cyclic irregularity had higher mean free T and acne grading scores when compared with those who had regular periods. Also they were younger than the patients with regular menstrual cyclicity. CONCLUSION: The levels of free T and E2 in male patients were higber than in normal control subjects. In the female patients the levels of DHT were higher than in the control subjects. However, there was no correlative relationship between hormonal parameters and the clinical severity of acne. Also the patients with irregular menstrual cyclicity had a higher free T and showed severe acne clinically.
Acne Vulgaris* ; Androgens ; Dihydrotestosterone* ; Estradiol* ; Female ; Humans ; Korea ; Male ; Periodicity ; Plasma* ; Testosterone*

Bcl-2 oncoprotein is being localized to mitochondria and interfering with programmed cell death (apoptosis) independent of promoting cell division in the lymphoid and nonlymphoid cells. The bcl-2 oncoprotein expression has been reported in follicular lymphomas as well as in diffuse non-Hodgkin's lymphoma, leukemia and a variable propotion of Hodgkin's lymphoma cases. Recent evidence suggests that some lymphomas protected from apoptosis is conferred through expression of Epstein-Barr virus(EBV) latent membrane protein which turn to cause upregulation of bcl-2. To define the role of the bcl-2 oncoprotein and EBV in lymphoid malignancy, we tried immunohistochemical studies with anti-bcl-2 antibody and In situ hybridization (ISH) with EBV-encoded small nuclear RNAs(EBER) in the paraffin embedded sections of 46 non-Hodgkin's lymphoma (NHL) cases and 20 Hodgkin's lymphoma (HL) cases. Bcl-2 oncoprotein expression was found in 37 of 46 cases (80%) of NHL with relatively strong cytoplasmic staining, and in 14 of 20 cases (70%) of HL with weak cytoplasmic staining in limited small numbers of RS, Hodgkin and lacunar cells. The widespread presence of bcl-2 oncogene in many different types of both NHL and HL supports that the extended cell survival through overexpression of bcl-2 gene protein may be a growth advantage of neoplastic lymphoid cells. In the ISH analysis for EBV, the presence of EBV was detected in 17 of 20 cases (85%) of HL, compared to 6 of 44 cases(13.6%) of NHL. It appears to be no direct correlation between overexpression of bcl-2 oncoprotein by neoplastic lymphoid cells and the presence of EBV in NHL but it seems to be a definite association between EBV and HL.

No abstract available.
Emergencies* ; Emergency Service, Hospital* ; Hospitals, General* ; Humans

No abstract available.
Hepatitis B Surface Antigens* ; Hospitals, General*

Poor marginal fidelity resulting in a large marginal gap increases plaque accumulation, gingival inflammation and dental caries. The purpose of this study was to evaluate the marginal fit of three different cervical finishing methods of prepared teeth. A stereomicroscope was used to measure the space between the margin of restoration and the finishing line of prepared tooth. The results were statistically analyzed using the ANOVA and Multiple Range Test(Tukey`s HSD). The results were as follows : 1. There were no significant differences concerning the types of tooth and position (P<0.05),whereas the differences were statistically significant in case of cervical finishing methods (P<0.05). 2. There were statistically significant differences between before and after cementation (P<0.05). 3. In comparison according to variable margin after cementation, the gap discrepancies were increased in 130degreeshoulder margin, chamfer margin and 90degreeshoulder margin in ascending order, and there were significant differences between 90degreeshoulder margin and chamfer, 130degreeshoulder margin 4. In comparison according to variable margins, the gap discrepancies were increased in chamfer margin, 130degreeshoulder margin and 90degreeshoulder margin in ascending order, and there were significant differences between 90degreeshoulder margin and chamfer, 130degreeshoulder margin 5. This study demonstrated a better marginal fit with all-ceramic crowns fabricated on chamfer and 130degreeshoulder margin compared with 90degreeshoulder margin.
Cementation ; Ceramics* ; Crowns* ; Dental Caries ; Inflammation ; Tooth