The authors evaluated the results of various operative methods for the treatment of axillary osmidrosis in 256 axillae of 132 patients. The methods studied herein are elliptical excision with(without Z-plasty, excision by modified W-plasty, undercutting of apocrine glands with preserving deep dermal vascular plexus (DDP) designed by the authors, undercutting of apocrine glands with ignoring deep dermal vascular plexus. With the undercutting methods, the skin incision was made by three different ways ', single vertical line, double vertical lines or single horizontal line in respect to the long axis of axillary vault. The results were as follows: 1. In the undercutting methods the stitch removals were done earlier than those of the excision methods by 3 to 8 days. The earliest stitch removal was in the undercutting methods with preservation of DDP done by single vertical incision (UPS). 2. Operative complications (wound disruption, flap necrosis or hematoma) occured in 12.9%. The wound disruption was main complication in the excision methods, the hematoma and flap necrosis in the undercutting methods. In the undercutting methods flap necrosis was seen only when DDP was ignored. 3. 123 axillae were followed up for more than 6 months after the operations. Among these the widely extended scars were seen in 94% of simple elliptical excisions(31 cases) and in 100% of excision with Z-plasty(16 cases) or modified W-plasty(3 cases). In the undercutting methods the scars were linear or invisible and best in UPS. 4. Success rate for the eradication of the malodor showed somewhat better results with undercutting methods compared with excisionmethods, but there was no statistical differences between the different groups. 5. In 6 axillae with the undercutting methods, histological examinations were done after more than three months. Apocrine glands were not seen in all the specimens. But some of them showed eccrine glands.
Apocrine Glands ; Axilla ; Axis, Cervical Vertebra ; Cicatrix ; Eccrine Glands ; Hematoma ; Humans ; Necrosis ; Skin ; Wounds and Injuries

The glucose uptake rate by plerocercoid of Diphyllobothrium sp. was a mean value of 5.35+/-0.80 micro-mole/hr/g of wet wt, and total CO(2) production rates by the plerocercoid larva averaged 7.54+/-0.73 micro-mole/hr/g of wet wt. The relative specific activity into respiratory CO(2) showed a mean value of 7.30 +/-0.90 per cent. The rate of CO(2) production derived from medium C(14)-glucose was a mean of 0.58+/-0.13 micro-mole/hr/g of wet wt. Therefore, the average value of 1.92+/-0.38 per cent of glucose utilized by the larvae from the medium C(14)-glucose was oxidized to respiratory CO(2). The tissue concentration of glycogen in plerocercoid larva was a mean of 46.28 +/-2.23 mg/g or 4.63+/-0.22 per cent/g of wet wt., and the turnover rate of glycogen pool was a mean of 0.049 +/- 0.012 %/hr or 0.010 +/- 0.003 mg/hr/g of wet wt. The average value of 2.76+/-1.00 per cent of glucose utilized by the larvae from the medium C(14)-glucose was incorporated to the glycogen. These data accounts for that only 5 per cent of the utilized glucose by the plerocercoid larvae participated in furnishing the oxidation into respiratory CO(2) and the synthetic process into glycogen.
parasitology-helminth-cestoda ; Diphyllobothrium sp. ; sparganum ; plerocercoid ; biochemistry ; autoradiography ; glucose ; metabolism ; CO(2)

OBJECTIVE: Leptin, a secreted protein of the Ob gene by white adipose tissue, plays an important role in the regulation of food intake and energy consumption in the brain and acts as a potential growth stimulator in normal and neoplastic breast cancer cells. However, a potential role of leptin as an endocrine regulator is unknown in ovarian cancer. In the present study, we investigated the expression of leptin and its receptors in various histologic types of ovarian cancer and immortalized ovarian cancer cell lines to find out potential effect of leptin on the cell growth and activation of ovarian cancer cell line. METHODS: The ovarian cancer tissues, serous (n=18), mucinous (n=15), clear cell (n=12) and endometrioid type (n=14), were used for immuno-histochemical staining for leptin (Ob) and leptin-receptors (Ob -R). Ovrian cancer cell lines (non-mucinous: SNU-8, OVCAR-3, and SKOV-3) and mucinous: MUC) were used for RT-PCR, Western blot analysis, and [H3] thymidine incorporation assay for the cell growth and activation of mitogen-activated protein kinase. RESULTS: Both long (Ob-Rb) and short (Ob-Rt) isoforms of leptin receptors are expressed in non-mucinous type of ovarian cancer tissues (serous, clear cell carcinoma and endometrioid cell carcinoma) and in non-mucinous ovarian cancer cell lines (SNU-8, OVCAR-3 and SKOV-3 cells). However, leptin and its receptors are not found in mucinous cancer cells and mucinous cancer cell line (MUC). In immunohistochemical staining, the immunreactive leptin is expressed on the nuclei of the stratified cuboidal-to-columnar epithelial cells whereas its receptor was sparsely expressed on the innermost epithelial cell clusters and cytoplasm in non-mucinous tumor. However, there are no immunoreactive leptin and its receptor expressions in the mucinous tumor. In addition, treatment with leptin resulted in the growth stimulation of ovarian cancer cell line, an activation of ERK 1/2 and inhibition of constitutive phosphorylation of p38 Mitogen-activated protein kinase (MAPK). CONCLUSION: Taken together, our data demonstrates preliminary that the expression of leptin and its receptor is different according to the cell types of the ovarian cancer. Also it canbe thought that leptin immunolocalized on the nuclei in non-mucinous type but not in mucinous possibly acts as a nuclear transcription factor. Further studies are necessary to validate whether leptin may be a potential regulator for ovarian cancer.
Adipose Tissue, White ; Blotting, Western ; Brain ; Breast Neoplasms ; Cell Line ; Cytoplasm ; Eating ; Epithelial Cells ; Leptin ; Mucins ; Neoplasms, Glandular and Epithelial ; Ovarian Neoplasms ; Phosphorylation ; Protein Isoforms ; Protein Kinases ; Receptors, Leptin ; Thymidine ; Transcription Factors

PURPOSE: Cyclooxygenase-2 (Cox-2) is the rate-limiting enzyme in the conversion of arachidonic acid to prostaglandins and can be induced by various agents such as growth factors and tumor promoters. Cox-2 contributes to carcinogenesis and tumor growth. This study was performed to demonstrate the correlation between elevated expression of Cox-2 and pathologic factors in breast cancer. METHODS: Cox-2 expression was analyzed immunohistochemically in paraffin-embedded tumor samples from 40 patients with breast cancer. Cox-2 expression was defined as negative or positive. The correlation between Cox-2 expression and pathologic factors (tumor size, axillary lymph node metastasis, TNM stage and histologic grade) was analyzed. RESULTS: Cox-2 was highly expressed in proportion to tumor size, but the difference was not significant (P>0.05). High Cox-2 expression was observed in the presence of axillary lymph node metastasis and TNM stage III, but was not significant (P>0.05). The Cox-2 expression rate was significantly associated with high histologic grade (I: 42.9%, II: 50.0%, III: 80.0%) (P=0.046). CONCLUSION: Elevated levels of Cox-2 expression were associated with large tumor size, presence of axillary lymph node metastasis, high TNM stage and high histologic grade, and can therefore be a possible marker for poor prognosis. Due to the small number of cases, we couldn't confirm the statistical significance except in terms of histologic grade. Further prospective studies with a large number of cases are required.
Arachidonic Acid ; Breast Neoplasms* ; Carcinogenesis ; Carcinogens ; Cyclooxygenase 2* ; Humans ; Intercellular Signaling Peptides and Proteins ; Lymph Nodes ; Neoplasm Metastasis ; Prognosis ; Prostaglandins

PURPOSE: Nonsteroidal antiinflammatory drugs (NSAIDs) have been shown to exist chemo preventive activity against colon cancers. In this study, we examined whether salicylate affects the survival of A549 cells, and investigated the presence of apoptosis. MATERIALS AND METHODS: We used A549 human lung cancer cell line. The measurement of cytotoxic concentration of salicylate was performed by MTT assay method. In order to test the involvement of apoptosis, we performed TUNEL assay, DAPI staining, flow cytometric analysis and RT-PCR. RESULTS: We showed that salicylate can potently induce apoptosis in A549 cells. A549 cells under went apoptosis in treatment with salicylate at pharmacological concentration (5 mM). CONCLUSION: Herein, our data provide a potential mechanism for chemopreventive activity of salicylate and suggest that salicylate may have therapeutic potential for the treatment of lung cancer.
Apoptosis* ; Cell Line ; Colonic Neoplasms ; Humans ; In Situ Nick-End Labeling ; Lung Neoplasms

Esophageal neuroendocrine carcinomas are very rare and have a poor prognosis. In addition, the optimum treatment has not been established due to the rarity of the condition. An accurate differential diagnosis is important because treatments and clinical courses differ from those of esophageal squamous cell carcinomas. We report a case of a 61-year-old female who underwent endoscopy for dysphagia and was diagnosed with a neuroendocrine carcinoma of the esophagus, which was successfully resected by esophagectomy.
Carcinoma, Neuroendocrine ; Carcinoma, Squamous Cell ; Deglutition Disorders ; Diagnosis, Differential ; Endoscopy ; Esophagectomy ; Esophagus ; Female ; Humans ; Neuroendocrine Tumors ; Prognosis

PURPOSE: Vitamin D3 was shown to arrest the growth of acute myelogenous leukemic cells and transforming growth factor- B1 (TGF- B1) was reported to be involved in the mechanism of vitamin D3. We studied the growth inhibitory effect of 1,25(OH)2-vitamin D3(C) and its analogue (EB1089) in leukemic cell lines and the changes in the secretion or the activation of TGF-B1 in the supernatant and the status of TGF-B1 type II receptor. MATERIALS AND METHODS: Growth inhibition by vitamin D3 and TGF-B1 in 5 leukemic cell lines (HEL, HL-60, U937, KG-1, K562) were assessed with clonogenic and [3H]thymidine assay respectively. TGF-B type II receptor status was examined by Southern and Northern blotting. The concentrations of TGF- B1 in the supernatant were quantitated by enzyme immunoassay. RESULTS: The growth of HEL, HL-60, U937 were inhibited in a dose-dependent fashion by both C and EB1089, more markedly by the latter. Anti-TGF-B neutralizing antibody partially reversed the growth inhibition. TGF-B1 markedly inhibited the growth of HEL, U937, KG-1, SNU-16 dose dependently while HL-60 and K562 showed no growth inhibition. HEL secreted latent TGF- 1 and HL-60 activated latent TGF- B1 or secreted active TGF-B1 irrespective of the treatment with vitamin D3. In U937, vitamin D3 increased the concentration of both active and latent TGF-B1. Deletion or abnormal expression of TGF- B type II receptor gene was not found in the 5 cell lines examined. CONCLUSION: Vitamin D3 has various pattern of growth inhibition in acute myelogenous leukemia and inhibits the growth of some cell lines by secretion or activation of TGF-B1. Abnormality of TGF-B type II receptor DNA or mRNA seems to be rare.
Antibodies, Neutralizing ; Blotting, Northern ; Cell Line ; Cholecalciferol ; DNA ; Immunoenzyme Techniques ; Leukemia, Myeloid, Acute ; RNA, Messenger ; Vitamin D

No abstract available.
Alzheimer Disease*

OBJECTIVE: Malignant gliomas with neuronal marker expression (MGwNM) are rare and poorly characterized. Increasingly diverse types of MGwNM have been described and these reported cases underscore the dilemmas in the classification and diagnosis of those tumors. The aim of this study is to provide additional insights into MGwNM and present the clinicopathological features of 18 patients. METHODS: We reviewed the medical records of 18 patients diagnosed as MGwNM at our institute between January 2006 and December 2012. Macroscopic total resection was performed in 11 patients (61%). We evaluated the methylation status of O6-methylguanine-DNA methyltransferase (MGMT) and expression of isocitrate dehydrogenase 1 (IDH-1) in all cases, and deletions of 1p and 19q in available cases. RESULTS: The estimated median overall survival was 21.2 months. The median progression-free survival was 6.3 months. Six patients (33%) had MGMT methylation but IDH1 mutation was found in only one patient (6%). Gene analysis for 1p19q performed in nine patients revealed no deletion in six, 19q deletion only in two, and 1p deletion only in one. The extent of resection was significantly correlated with progression free survival on both univariate analysis and multivariate analysis (p=0.002 and p=0.013, respectively). CONCLUSION: In this study, the overall survival of MGwNM was not superior to glioblastoma. The extent of resection has a significant prognostic impact on progression-free survival. Further studies of the prognostic factors related to chemo-radio therapy, similar to studies with glioblastoma, are mandatory to improve survival.
Classification ; Diagnosis ; Disease-Free Survival ; Glioblastoma ; Glioma* ; Humans ; Isocitrate Dehydrogenase ; Medical Records ; Methylation ; Multivariate Analysis ; Neurons*

Gasritis cystica profunda (GCP) is a rare disease in which hyperplasia of mature glandular epithelium extends into the tissues beneath the submucosa. It shows multiple small cysts in the mucosa and submucosa of the stomach. It was firstly reported by Littler and Gleibermann on 1972. GCP is mainly observed at the site of gastroenterostomy but, it may occur in the stomach without a previous history of surgery. The proposed pathogenesis of the these abnormalities are related to ischemia, chronic inflammation and the presence of a foreign body. GCP may present not only as a submucosal tumor or as solitary or diffuse polyps but also as a giant gastric mucosal fold rarely. It should be differentiated from Menetrier's disease, Zollinger-Ellison syndrome, inflammatory disease and malignancy. We present a case of gastritis cystica profunda without having had any previous surgery, suspiciously caused by gastric foreign body. We made a diagnosis based on findings from the esophagogastroduodenoscopy, endoscopic ultrasonography and histologic findings after surgery.
Diagnosis ; Endoscopy, Digestive System ; Endosonography ; Epithelium ; Foreign Bodies* ; Gastritis* ; Gastritis, Hypertrophic ; Gastroenterostomy ; Hyperplasia ; Inflammation ; Ischemia ; Mucous Membrane ; Polyps ; Rare Diseases ; Stomach ; Zollinger-Ellison Syndrome