No abstract available.
Blood Coagulation Factors* ; Fibrinogen*

No abstract available.
Blood Coagulation Factors* ; Fibrinogen*

OBJECTIVES: As one of the possible mechanisms of viral evasion in the HPV-infected cervical cancer cells, the role of amino acid sequence change in epitope region has not been reported yet. In this study, sequence variations of HPV 16 E6, E7 gene, especially focused on epitope region, were analysed, the status of immunomodulatory factors were documented, and finally the possible correlation between the sequence variations and the loss of HLA class I expression was examined. METHODS: The entire ORF(open reading frame)s of HPV 16 E6, E7 were sequenced by the fluorescent dideoxy termination method. In addition proteins and transcripts of HLA-ABC, beta2-microglobulin(beta2-m), TAP (transporter associated with antigen processing), and LMP(large multi-functional proteasome) were evaluated by immunohistochemistry and RT-PCR, respectively in 40 clinical specimens of primary cervical cancer and 6 cervical cancer cell-lines. Medical records including pathologic reports were reviewed. RESULTS: Among the 27 cases confirmed as harboring HPV 16 DNA, only one(3.7%) found as a prototype. Among 11 kind of variants identified in total, 4 variants(5 nucleotide sites) which were never reported before has been found, registered firstly to GenBank. The most frequently found one(16 cases, 59.3%) contains D25E, N29S in E6, E7 region, respectively and the most common variation in E6, E7 ORFs found concurrently(p<0.05). Down-regulation of HLA-ABC and beta2-m was identified in 32(86.5%) and 35 cases(89.7%), respectively and transcripts of TAP, LMP were identified in over 85% of cases. However, there was no significant difference in HPV 16 infection, D25E in E6 and so on between HLA-ABC, beta2-m positive and negative groups. The well-known clinicopathologic parameters did not correlate with sequence variations and immunomodulatory factors. Five sequence variations in HPV 16 E6, E7 ORFs that were not previously reported worldwide were found, registered firstly to GenBank. CONCLUSION: It seems that multiple mechanisms are operated in down-regulation of HLA class I molecules and the phenotypic profile of immunomodulatory factors seems to be unrelated in vivo to the naturally occurring HPV 16 E6, E7 variations in epitope region.
Amino Acid Sequence ; Animals ; Databases, Nucleic Acid ; DNA ; Down-Regulation ; Ecthyma, Contagious ; Female ; Human papillomavirus 16 ; Humans* ; Immunohistochemistry ; Medical Records ; Open Reading Frames ; Uterine Cervical Neoplasms*

No abstract available.
Drug Therapy* ; Stomach Neoplasms*

Prolapse of bladder through vesico-vaginal fistula is quite a rare urological problem. A 38-year-old woman was admitted to the Woo Sok University Hospital with a baby head sized round mass attached to the vagina. Patient had a prolapse of uterus after having her second child birth about 10 years ago and cauterization with corrosives for the treatment of prolapse of uterus was undergone for two times. About one year prior to the admission, the prolapse of uterus recurred and cauterization with corrosives was tried again, resulting vesico-vaginal fistula through which the bladder was everted and prolapsed. Under the general anesthesia, the everted and prolapsed bladder was reduced manually in normal position and vesico-vaginal fistulectomy was performed with good result.
Adult ; Anesthesia, General ; Caustics ; Cautery ; Child ; Female ; Fistula* ; Head ; Humans ; Parturition ; Prolapse* ; Urinary Bladder* ; Uterus ; Vagina ; Vesicovaginal Fistula

The simple chest X-ray study is essential in diagnosis of pulmonary tuberculosis, especially primary pulmonarytuberculosis. Authers analyzed the chest films and tuberculin tests of 521 cases which are suspected primarypulmonary tuberculosis coinically, from Jan. 1978 to Nov. 1982 at Seoul Red Cross Hospital. The results are asfollows; 1. Among the total 521 cases, the 115 cases of chst film reveal 245 probable pulmonary tuberculouslesions. 2. The coincidence of the parenchymal and lymphnodal involvement are observed in 40 cases (34.8%) with137 lesions(55.9%), parenchymal only in 23 cases (20.0%) with 25 lesions(10.2%) and lymphnodal only in 52 cases(45.2%) with 83 lesions(33.9%). 3. In observing the distribution of the 83 parenchymal lesions, the right lung istwo times as common as that of the left, being the highest in the RLL field with 41 lesions(49.4%), the lowest inthe LLL field with 3 lesions(3.6%). 4. In observing the distribution of the 162 lymphnodal lesions, there are 95lesions(58.6%) in the right and 67 lesions(41.4%) in the left, involving hilar nodes with 150 lesions(92.6%) andmediastinal nodes with 12 lesions(7.4%). The incidence of unilateral involvement is 13 cases (11.3%) and bilateral79 cases (68.7%). 5. Among the total 245 lesions, 141 lesions(57.9%) are smaller than rice in size, 120lesions(49.0%) are amophous in shape and 186 lesions(75.9%) are mainly calcific in density. According to theincrease of age, count and detection rate of the lesion on the chest film are increased. 6. Tuberculin testsshowed 26.1% negative rate in 115 tuberculous cases, and 51% positive rate in 406 negative chest cases.
Diagnosis ; Incidence ; Lung ; Red Cross ; Seoul ; Thorax ; Tuberculin ; Tuberculin Test ; Tuberculosis ; Tuberculosis, Pulmonary

Therapeutic plasma exchange is used in almost every condition in which there is a plasma factor thought possibly to the etiology or pathogenesis of a disease or one of its manifestations. In order to evaluate plasma exchange using fresh frozen plasma as replacement solution, eighty four therapeutic plasma exchanges were carried out in eighteen patients. In standardized procedures, 1.5 times the calculated plasma volume was replaced with a Hartman's solution and fresh frozen plasma. Anticoagulation was achieved using a whole venous blood to 2.5% trisodium citrate in the ratio of 10 to 1. Total calcium, phosphorus, glucose, urea nitrogen, creatinine, bilirubin, alkaline phosphatase, amylase, creatine kinase, IgG, C3, total white and red blood cell count, hemoglobin, and differential count were not significantly affected by the procedure. In contrast, serum cholesterol, total protein, albumin, aspartate aminotransferase, alanine aminotransferase, ionized calcium, IgM, C4 and platelet were significantly decreased by the plasma exchange. All these measurements had returned to the first pre-exchange level within 24 hours, while the C4 and platelet count took between 24 and 72 hours, and the IgM level, between 72 hours and 1 week. These data indicated that in an isovolemic plasma exchange there was a transient but rapidly reversible effect on all the components studied, with C4 and platelet count, returning more slowly to pre-exchange level than the others, and IgM levels responding the slowest. In summary, plasma exchanges using fresh frozen plasma as replacement solution were assumed to be not significantly affected the function of various organs.
Alanine Transaminase ; Alkaline Phosphatase ; Amylases ; Aspartate Aminotransferases ; Bilirubin ; Blood Platelets ; Calcium ; Cholesterol ; Citric Acid ; Creatine Kinase ; Creatinine ; Erythrocyte Count ; Glucose ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Nitrogen ; Phosphorus ; Plasma Exchange* ; Plasma Volume ; Plasma* ; Platelet Count ; Urea

No abstract available.

No abstract available.

No abstract available.
Chromosome Aberrations*