2.Intrageneric Relationships of Trichoderma Based on Internal Transcribed Spacers and 5.8S rDNA Nucleotide Sequences.
Gi Young KIM ; Goang Jae LEE ; Myung Gyu HA ; Tae Ho LEE ; Jae Dong LEE
Mycobiology 2000;28(1):11-16
The nucleotide sequences of the internal transcribed spacer (ITS) regions of the ribosomal DNA including the 5.8S ribosomal RNA gene (rDNA) have been determined for 11 species in order to analyze their intrageneric relationships. The total length of these sequences ranged from 530 nucleotides for Trichoderma reesei KCTC 1286 to 553 nucleotide for Trichoderma koningii IAM 12534. Generally speaking, the length of ITS1 region was about 30 nucleotides longer than that of the ITS2 region. Also, the sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites were also found. Thus, a neighbor-joining tree was constructed using the full sequence data of the ITS regions and the 5.8S rDNA. The Trichoderma genus used to be grouped on the basis of the morphological features and especially the shape of phialides needs to be reexamined. The phylogenetic tree displayed the presence of monophylogeny in the species of Trichoderma. Therefore, it was difficult to distinguish the intrageneric relationships in the Trichoderma genus.
Base Sequence*
;
DNA, Ribosomal*
;
Nucleotides
;
Phylogeny
;
RNA, Ribosomal, 5.8S
;
Trichoderma*
3.Usefulness of Computed Tomographic Angiography in the Detection and Evaluation of Aneurysms of the Circle of Willis.
Hyuk Gi LEE ; Jae Hoon CHO ; Sung Lak LEE ; Dong Gee KANG ; Sang Chul KIM
Journal of Korean Neurosurgical Society 2000;29(3):345-352
No abstract available.
Aneurysm*
;
Angiography*
;
Circle of Willis*
4.A case of fetal cystic hygroma colli.
Jung Don PARK ; Jong Gi LEE ; Kyung Il CHO ; Heon Soo LEE ; Jae Bok PARK
Korean Journal of Obstetrics and Gynecology 1993;36(7):1993-1998
No abstract available.
Lymphangioma, Cystic*
5.Epidemiological Characteristics of Field Tick-Borne Pathogens in Gwang-ju Metropolitan Area, South Korea, from 2014 to 2018
Jung Wook PARK ; Seung Hun LEE ; Gi Seong LEE ; Jin Jong SEO ; Jae Keun CHUNG
Osong Public Health and Research Perspectives 2020;11(4):177-184
The importance of tick-borne diseases is increasing because of climate change, with a lack of long-term studies on tick-borne pathogens in South Korea. To understand the epidemiological characteristics of tick-borne diseases, the monthly distribution of field ticks throughout the year was studied in South Korea between May 2014 and April 2018 in a cross sectional study. The presence of various tick-borne pathogens ( During the study period there were 11,717 ticks collected and 4 species identified. In conclusion, due attention should be paid to preventing tick-borne infections in humans whilst engaged in outdoor activities in Spring and Autumn, particularly in places where there is a high prevalence of ticks.
6.Study on the blood estradiol level and follicle development in induced superovulation of insufficient menstrual cycles.
Seung Gwan CHOI ; Jae Myeoung KIM ; Chung Soon BAIK ; Gi Soon LEE ; Byung Hee SUH ; Jae Hyun LEE
Korean Journal of Obstetrics and Gynecology 1992;35(2):256-271
No abstract available.
Estradiol*
;
Female
;
Menstrual Cycle*
;
Superovulation*
7.Experimental Corneoacleral Limbal Wound Repair with Cyanoacrylate Glue and 8-0 Vicryl on Rabbits.
Jae Sun LEE ; Sung Gi CHO ; Sang Kyoung CHOI
Journal of the Korean Ophthalmological Society 1989;30(6):899-905
To evaluate the availability of n-butyl-2-cyanoacrylate as an adhesive in limbal wound repair, we used 15 rabbits(30 eyes). A limbal incision exactly 9mm long was made and the wound of one eye was tightly closed with seven equally apaced corneoscleral sutures of 10-0 nylon(nylon group), The wound of the other eye was closed by two(6.5mm spaced, 11 and 1 o'clock) corneoscleral sutures of 8-0 vicryl and the outer surface of this limbal wound was thinly coated with a small amount of Histoacryl(Histo-acryl group). The average disrupting pressure in nylon group was 0.87 +/- 0.11 kg/cm2 and that in Histoacryl group was 0.79 +/- 0.17 kg/cm2. The average refractive change in nylon group was 2.5 diopter with-the-rule astigmatism, and that in Histoacryl group was 0.9 diopter against-the-rule astigmatism. A moderate inflammatory reaction was noted at the histopathologic findings of the wounds closed with Histoacryl 14 days after operation. From the results, post-operative inflammatory changes indicated that the Histoacryl seemed to be rather unsuitable for clinical application.
Adhesives*
;
Astigmatism
;
Cyanoacrylates*
;
Enbucrilate
;
Nylons
;
Polyglactin 910*
;
Rabbits*
;
Sutures
;
Wounds and Injuries*
8.A Study on the Necessity to Revise the Present Growth Data for Height and Weight?.
Gi Dong HWANG ; Jae Kyung CHOI ; Jeh Hoon SHIN ; Nam Soo KIM ; In Joon SEOL ; Hahng LEE
Journal of the Korean Pediatric Society 1995;38(6):745-751
No abstract available.
9.Diagnostic Values of pH of Vaginal Discharge, Amine Test, and Microscopic Examination of Infectious Vaginitis.
Jae Dong CHOI ; Gi Duk KIM ; Min Whan KOH ; Tae Hyung LEE ; Wun Yong CHUNG
Yeungnam University Journal of Medicine 1988;5(2):129-139
Vaginitis is one of the most common disease in gynecologic field in recent days. About 90% of these patients suffer from the infection of the vagina caused by Gardnerella vaginalis, Candida, or Trichomonas, either alone or in combination. For the effective diagnosis and management, it is essential to get an accurate identification of the causative agent. Applying simple and easy diagnostic methods such as pH of vaginal discharge, amine test and microscopic examination of wet mount preparation of normal saline and 10% KOH to 549 cases of randomly selected patients visiting Out-patient Department of Obstetrics and Gynecology of Yeungnam University Hospital through May 1st to Sept. 31st, 1987, the following results were obtained. 1. In the Gardnerella vaginitis vaginal pH was more than 5.0 in 81.0% of total cases, positive amine test in 62.8%, decreased lactobacilli in 77.4% & decreased WBC counts in 70.1%, In the Trichomonas vaginitis vaginal pH more than 5.0 was noted in 93.3% of the cases, negative amine test in 90%, decreased showed vaginal pH of 3.0~4.0 in 83.2%, negative amine test in 90%, decreased lactobacilli in 90% & increased WBC counts in 93.3%, whereas Candida vaginitis and normal groups showed vaginal pH of 3.0~4.0 in 83.2%, negative amine test in 100%, normal distribution of lactobacilli in 89.7%, normal WBC counts in 72.4%. 2. The accuracy rates of physical diagnosis by wet mount preparation of normal saline and 10% KOH revealed 26.3%, 47.5%, 70.0% in Gardnerella vaginitis, Candida vaginitis, and Trichomonas vaginitis, respectively. The vaginal pH and amine test showed 83.2% of accuracy rate in Candida vaginitis group, 60.6% in Gardnerella vaginitis group and 83.3% in Trichomonas vaginitis group. 3. In 23 cases of Gardnerella vaginitis showing vaginal pH of 3.0 or 4.0 and positive amine test, the clue cells were observed in 10% or less in 12 cases, 30% in 5 cases, 50% in 4 cases and 50% or more in 2 cases. In summary vaginal pH and amine test could be useful in screening and differentiating the different types of infectious vaginitis. Furthermore by adding microscopic examination of normal saline and 10% KOH wet mount preparation to them, they not only could obtain higher diagnostic accuracy rate but would be more valuable in selecting the patients requiring cultures to confirm the diagnoses.
Candida
;
Diagnosis
;
Gardnerella
;
Gardnerella vaginalis
;
Gynecology
;
Humans
;
Hydrogen-Ion Concentration*
;
Mass Screening
;
Obstetrics
;
Outpatients
;
Trichomonas
;
Trichomonas Vaginitis
;
Vagina
;
Vaginal Discharge*
;
Vaginitis*
10.EFFECT OF VARIOUS GROWTH FACTORS IN CULTURE OF EAR CHONDROCYTES OF RABBIT.
Sang Hoon HAN ; Jeong Hoon KANG ; Chang Gi SUNG ; Jae Dam LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(5):884-892
No abstract available.
Chondrocytes*
;
Ear*
;
Intercellular Signaling Peptides and Proteins*