PURPOSE: This study was designed to evaluate usefulness of intravenous digital subtraction angiography (IV-DSA) in detection of peripheral arterial obstructive disease(PAOD) and in follow-up of percutaneous transluminal angioplasty(PTA). MATERIALS AND METHODS: 35 Patients who had clinical symptoms and signs of compromised lower extremity perfusion, was screened with IV-DSA and then performed confirmative conventional angiography. We obtained sensitivity, specificity and accuracy of IV-DSA by comparing the findings of IV-DSA with those of conventional angiography. 21 patients who had been performed successful PTA, were foliowed-up with IV-DSA in 3, 6, 12months. We studied patency rate and factors that influenced restenosis. RESULTS: The sensitivity, specificity and accuracy of IV-DSA were 100%, 97%, 97% in lilac artery, 92%, 96%, 93% in femoropopliteal artery, 85%, 75%, 70% in proximal tibioperoneal artery retrospectively. IV-DSA follow up after PTA showed patency rate of 67% in first 3month, 67% in 2nd 3month, 60% in next 6month. Longer length and more irregular surface of the stenotic site, and higher incidence of run-off of vessel and of residual stenosis in the patients with restenosis were noted. CONCLUSION: High sensitivity, specificity and accuracy of IV-DSA in evaluating PAOD suggest that IV-DSA is useful in screening, planning therapy and following up after PTA.
Angiography ; Angiography, Digital Subtraction ; Arterial Occlusive Diseases* ; Arteries ; Constriction, Pathologic ; Follow-Up Studies ; Humans ; Incidence ; Lower Extremity ; Mass Screening ; Perfusion ; Retrospective Studies ; Sensitivity and Specificity

No abstract available.
Humans ; Spondylolisthesis*

No abstract available.
Hematoma* ; Orbit*

No abstract available.
Cerebral Hemorrhage* ; Mucormycosis*

A multitude of methods for correction of the residual cleft lip nose deformity have been developed. Inspite of the development it appears that no one procedure has achieved satisfactory results. In addition, the appropriate timing for surgical correction still remains unsettled. The philtrum plays a keyhole in the appearance of the upper lip and nostril sill. But the formation of philtrum with muscle flap or conchal cartilage have been resulted in a unsatisfactory aesthetic outcome. We performed 26 cases of the correction of unilateral cleft lip nose deformity between May 1993 and September 1996, and we always used autogenous palmaris longus tendon for reconstruction of philtral column and nostril sill augmentation. Palmaris longus tendon creates a more definite philtral unit comparing to other materials which have been used and it is easy to harvest and transfer and it does not affect normal function of the hand. And we followed up all patients and confirmed that disappearance of newly formed philtrum is rare. In conclusion, this study shows that autogenous palmaris longus tendon is a useful material for reconstruction of philtral column.
Cartilage ; Cleft Lip* ; Congenital Abnormalities* ; Hand ; Humans ; Lip ; Nose* ; Tendons*

No abstract available.
Heart Diseases* ; Heart*

Kyphotic deformity of thoracic or lumbar spine is generally developed as a consequence of Scheuerman's kyphosis, ankylosing spondylitis, congenital kyphosis, tuberculous kyphosis, spinal tumor, senile kyphosis, trauma and extensive laminectomy etc. The method of treatment is usually conservative. However, in severe kyphosis, gross abnormaility and neurologic complications, and the compression of cardiopulmonary system are resulted and then surgery may be indicated. Since the report of spinal osteotomy by Smith-Peterson, several treatment options have been reported. Despite improved techniques for treating spinal kyphotic deformities, correction of severe and acutely angled deformities are less effective and resulting many complications. We propose the use of a new method of posterior closing spinal osteotomy for the treatment of severe and acutely angled kyphotic deformity to minimize the complications and for effective correction. We report the technique and results of this method in two cases of tuberculous kyphosis and a case of ankylosing spondylitis.
Congenital Abnormalities ; Kyphosis ; Laminectomy ; Methods ; Osteotomy ; Spine ; Spondylitis, Ankylosing

A 65-year-old man was admitted due to low grade fever, dry coughing, and dyspnea on exertion. The chest radiograph and CT scan showed diffuse ground glass opacities and small nodules in the both lung fields resulting in a diagnosis of severe interstitial pneumonia. Conservative treatment with antibiotics and bronchodilators decreased the symptoms, but the dyspnea and cough reappeared when he returned home. An inspection of his house revealed the presence of fungi under the wallpaper. His symptoms disappeared completely after these were removed. His clinical course raised the suspicion of hypersensitivity pneumonitis and these fungi believed to be the cause of hypersensitivity pneumonitis. The histological findings of a lung specimen by video-assisted thorachoscopy were compatible with hypersensitivity pneumonitis. The fungi were identified as Alternaria.
Aged ; Alternaria* ; Alveolitis, Extrinsic Allergic* ; Anti-Bacterial Agents ; Bronchodilator Agents ; Cough ; Diagnosis ; Dyspnea ; Fever ; Fungi ; Glass ; Humans ; Hypersensitivity* ; Lung ; Lung Diseases, Interstitial ; Radiography, Thoracic ; Tomography, X-Ray Computed

Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. A. oryzae is an important microorganism for industrial production of enzymes and fermented food products. It secrets large quantities of proteins or enzymes into the culture medium which makes this organism appealing for the production of heterologous proteins. Recently Electric field-mediated transformation method, electroporation, has been applied to fungal transformation. It is fast, simple to handle, and avoids the use of some chemicals. The optimum conditions for A. oryzae were determined with pILJ-16 and ~0.2 x 105 protoplast cell at various field strength. The survived population of protoplasts in the electric field were ~80% of nonprotoplast cell population at 1.3 KV/cm to ~50% at 6.3 KV/cm. The electrotransformation efficiency, expressed as transformants/microgram of input DNA/population of protoplast cells, increased with the increment of the field strength up to 6.3 KV/cm. The highest value, 14.35%, was obtained at 6.3 KV/cm and 1540ohm. Some antibiotics for the dominant selectable makers were applied to A. oryzae and Tolypocladium inflatum. Whereas phleomycin was very effective on T. inflatum, hygromycin B and phleomycin were not effective on A. oryzae. Protoplasts were obtained with hemicellulase and celluclast, instead of novozyme234. More than 104 transformants/microgram of DNA with hemicellulase-treated protoplasts were obtained by using electroporation at the condition of 2,500 voltage, 1,540 ohm and 0.50 capacitance. Less than 102 transformants/microgram of DNA were obtained with Novozyme234- and celluclast-treated protoplasts.
Anti-Bacterial Agents ; Ascomycota ; Aspergillus oryzae* ; Aspergillus* ; DNA ; Electroporation ; Fungi* ; Hygromycin B ; Oryza ; Phleomycins ; Protoplasts

Osteoporosis is a disease characterized by low bone mass and micro- architectural deterioration of bone tissue, leading to enhanced bone fragility and a consequent increase in fracture risk. Women are the most likely to suffer osteoporotic fractures. In the second part of life bone mass decreases slowly, starting at about the age of 40 for cortical bone and somewhat earlier for trabecular bone. The total loss will be about 50% of the cancellous bone and 35% of the cortical bone in women. The imbalance between the bone resorption and bone formation can be caused by a decrease in the amount of new bone formed. The biochemical causes of the negative balance are not known. Therefore we studied to know the cause of negative balance after postmenopausal state by histomorphometry and in situ hybridization(ISH) for osteopontin. 84 female Spraque-Dawley rats, weighing approximately 200g, and fed laboratory chow ad libitum, were used in this study. Among those, 42 rats were sham operated, and 42 rats in experimental group were ovariectomized. They were divided into 7 groups consisting of 6 rats/group, and were serially sacrified on the 3, 5, 7, 10, 14, 30, and 60 days after operation and we obtained both femur of rats. After specimen preparation as usual manner, it was stained with H&E, MT. For histomorphometrical study, the bone structure was evaluated using the following parameters with OPTIMAS soft ware: trabecular bone volume/tissue volume(BV/TV, %), trabecular thickness(Tb.Th., micrometer), trabecular separation(Tb.Sp., micrometer). And in the way of ISH(in situ hybridization) procedure, osteopontin mRNA expressing cell was calculated. Statistical differences between OVX and sham-operated rats were evaluated using the ANOVA. After examination and comparision of all specimens, the results of this study were as follows: 1. BV/TV(%) after ovariectomy was decreased to 46.2+/-6.75%, compared with 58.14+/-8.72% of control group, with no statistical significance. These declination continued to 14 days postsurgery, in 30 and 60 days, it was decresed to 14.01+/-4.07% and 11.4+/-4.73% respectively, compared with 47.2+/-8.79% and 48.8+/-7.54% of control group, with statistical significance detected. 2. Trabecular thickness(micrometer) was decresed from 3 days to the remaining periods, with no statistical significance. 3. Trabecular separation(micrometer) was incresed throughout the study, especially in 60 days postsurgery, control group was 124.2+/-7.34micrometer and experimental group was 319.5+/-15.85micrometer, with statistical significance detected at 60 days postsurgery (p<0.05). 4. Osteopontin mRNA expressing cells was decreased from 3 days postsurgery, compared with 92.3+/-5.25 of control group. And these declination was observed throughout the study. Especially in 60 days postsurgery, control group was 93.25+/-2.72 and experimental group was decresed to 24.3+/-6.24, with statistical significance detected(p<0.05). From above results, after ovariectomy, bone formation was decreased, and it is thought that these bone formation declination was due to supression of osteopontin synthesis, which is the one of the bone matrix proteins.
Animals ; Bone and Bones ; Bone Matrix ; Bone Resorption ; Female ; Femur* ; Humans ; Osteogenesis ; Osteopontin* ; Osteoporosis ; Osteoporotic Fractures ; Ovariectomy ; Rats* ; RNA, Messenger*