In this study, the feasibility of identification and genotypic differentiation of enteroviruses was investigated by using nested reverse transcription-polymerase chain reaction (nested RT-PCR), single-stranded conformation polymorphism (SSCP), and restriction fragment length polymorphism (RFLP) techniques. Two hundred seventy-four clinical samples were assayed by both nested RT-PCR and tube culture method using MRC-5 and MK cells; 58 (86.6%) out of 67 enterovirus culture-positive samples contained enteroviral RNA. In addition, 114 (55.1%) of 207 samples from patients with suspected enteroviral CNS disease with negative viral cultures were positive by the nested RT-PCR. The nested RT-PCR products were genotyped by the SSCP method and the results were compared with serotypes. We could differentiate 6 subtypes, 3 of which are similar to coxsackievirus B3, B5, echovirus 11, plus 3 other subtypes. RFLP cleaved with Sty I, Bgl I, and Xmn I yielded characteristic patterns for each laboratory strains. This study demonstrates the usefulness of the RT-PCR for the rapid diagnosis of enterovirus infection and the potentials of the SSCP method for differentiation of enterovirus strains.
Central Nervous System Diseases ; Diagnosis ; Enterovirus B, Human ; Enterovirus Infections ; Enterovirus* ; Genotype* ; Humans ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single-Stranded Conformational ; RNA

No abstract available.
Choriocarcinoma* ; Female ; Pregnancy

No abstract available.
Gangwon-do* ; Humans

No abstract available.
Hepacivirus* ; Hepatitis C* ; Hepatitis* ; Korea* ; Prevalence*

No abstract available.
Gangwon-do* ; Humans ; Norovirus* ; Sequence Analysis*

No abstract available.
Hepatitis E virus* ; Hepatitis E* ; Hepatitis* ; Korea* ; Prevalence*

No abstract available.
Candida albicans* ; Candida* ; Ecthyma* ; Giant Lymph Node Hyperplasia* ; Humans ; Proteus vulgaris* ; Proteus*

Neurilemmomatosis is a clinical entity clearly distinguished from neurofibromatosis of von Recklinghausen's disease, and characteristically consists of multiple, cutaneuos neurilemmomas, central nervous system tumors, and neurologic disorders. A 52-year-old man, with multiple spinal cord neurilemmomas and unilateral acoutic neurilemmoma, presented with multiple subcutaneous nodules on the flexor parts of the extremities. Histologically, it was diagnosed as neurilemmoma. We report a case of neurilemmomatosis associated with CNS tumors.
Central Nervous System Neoplasms ; Extremities ; Humans ; Middle Aged ; Nervous System Diseases ; Neurilemmoma ; Neurofibromatoses ; Neurofibromatosis 1 ; Spinal Cord

No abstract available.
Antibodies, Monoclonal* ; Borrelia burgdorferi* ; Borrelia* ; Lyme Disease*

We present the first reported case of cold urticaria, which was mistaken as congestion status, in a patient undergoing breast reconstruction with deep inferior epigastric perforator (DIEP) flap coverage. A 53-year-old woman underwent an immediate bilateral breast reconstruction with DIEP flap coverage. The harvested DIEP flap was wrapped with wet gauze and kept above an ice pack to minimize ischemic damage before flap in-setting to the mastectomy site. After the transfer of the harvested DIEP flap, she had suddenly developed an erythematous and edematous lesion, specifically on the flap. The room temperature was raised and a single dose of steroid and antihistamine was given intravenously. At the first instance, flap congestion was suspected; however, as the rash gradually spread throughout the body, we could deduce that it was cold urticaria. The erythematous rash and whirl resolved soon after receiving medication. Patients with known cold urticaria undergoing surgical procedures require several precautions (e.g., antihistamine, relatively warm operation room) to lessen the risk of severe reaction. Especially in patients with DIEP free flap, the lack of preoperative treatment could result in a pinkish flap due to urticaria, which could be mistaken by the surgeon as a congested flap.
Breast* ; Estrogens, Conjugated (USP)* ; Exanthema ; Female ; Free Tissue Flaps* ; Humans ; Hyperemia ; Ice ; Mammaplasty* ; Mastectomy ; Middle Aged ; Urticaria*