No abstract available.
Anemia* ; Bone Marrow Cells* ; Bone Marrow* ; Child* ; Humans

We compared 87 portographic filling defects detected by portal CT in 64 patients were compared with those obtained by indirect portography. The indirect portography could visualize portogram only in anterior-posterior view. But the portal portal CT could visualize both portogram and hepatogram. We examined the portal CT and indirect portography and compared the accuracy of the both methods to evaluate the limitation and significance of the indirect protography. The mass shape lesions were seen on the portal CT which means portal flow defects of the mass lesions only could not depict totally in indirect portography (0%, 0/41). And the larger defects than real mass lesion were seen in portal CT means mass with associated portal flow defect and find portal vein invasion around the mass in 52% (24/46) of the indirect portography. Among them, only 66% of mass were detected correctly in the indirect portography comparing with mass lesion in portal CT. In summary, indirect portogram could not detect small filling defects which detected in portal CT and could not depict the extent of large filling defects. It also could not visualize correctly the protal flow in non-lesion side of the liver parenchyma.
Humans ; Liver ; Portal Vein ; Portography*

No abstract available.
Splints* ; Sterilization, Tubal*

No abstract available.
Child* ; Humans

p53 gene mutation is commonly accepted to be associated with loss of negative cell cycle control and progression of tumors. The proliferative activity of tumor cells is considered to be a valuable indicator of tumor aggressiveness. This study is intended to compare p53 protein expression with cell proliferation rates in the ovarian epithelial tumors according to the various clinicopathological parameters. Immunohistochemistry using monoclonal p53 antibody (DO-1) and PCNA antibody (PC10) was applied to 56 cases of ovarian epithelial tumors including 17 cases of borderline tumor. The results were as follows. Both immunohistochemical staining of PCNA and p53 protein showed positive reactions confined to the nuclei of tumor cells. There were significant differences of p53 protein expression rates between borderline malignancies (11.8%) and cystadenocarcinomas (56.4%) of ovary. The expression rate of p53 protein was not significantly different according to the differentiation and the stage, but the cases of strong positive reaction to p53 protein were more frequently noted in the poorly differentiated and advanced staged tumors. The PCNA indices of p53 strong positive cases were higher than those of p53 weak positive cases. In summary, p53 protein and PCNA expression may be used as an adjuvant in differentiating borderline lesions from carcinomas of ovary and predicting their biological behaviors.
Cell Cycle Checkpoints ; Cell Proliferation ; Cystadenocarcinoma ; Female ; Genes, p53 ; Immunohistochemistry ; Ovary ; Proliferating Cell Nuclear Antigen*

No abstract available.
Child* ; Endoderm* ; Endodermal Sinus Tumor* ; Humans

[Objective] The aim of this study was to investigate the effects of Joksamni (ST 36) combination on NADPH-diaphorase, neuronal nitric oxide synthase (nNOS), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) in the cerebral cortex of spontaneously hypertensive rat.
[Methods] The experimental groups were divided into four groups : Normal, Joksamni (ST 36), Joksamni (ST 36) + Eumneungcheon (SP 9), and Joksamni (ST 36) + Gokji (LI 11). Needles were inserted into acupoints at the depth of 0.5 cm with basic insertion method. Electroacupuncture was done under the condition of 2 HZ electrical biphasic pulses with continuous rectangular wave lasting for 0.2 ms until the muscles produced visible contractions. Such stimulation was applied continuously for 10 minutes, 1 time every 2 days for 10 sessions of treatments. Thereafter we evaluated changes in NADPH-d positive neurons histochemically and changes in nNOS, NPY and VIP positive neurons immunohistochemically.
[Results] The optical densities of NADPH-d positive neurons of the Joksamni (ST 36) + Eumneungcheon (SP 9) group in all areas of cerebral cortex and Joksamni (ST 36) + Gokji (LI 11) group in primary somatosensory cortex, visual cortex, auditory cortex, perirhinal cortex were significantly increased as compared to the Joksamni (ST 36) group.
The optical densities of NADPH-d positive neurons of the Joksamni (ST 36) + Gokji (LI 11) group were sig-nificantly decreased as compared to the Joksamni (ST 36) + Eumneungcheon (SP 9) group with the exception of primary somatosensory cortex.
The optical densities of nNOS positive neurons of the Joksamni (ST 36) + Eumneungcheon (SP 9) group in all areas of cerebral cortex and Joksamni (ST 36) + Gokji (LI 11) group in auditory cortex, perirhinal cortex, insular cortex were significantly increased as compared to the Joksamni (ST 36) group. The optical densities of nNOS positive neurons of the Joksamni (ST 36) + Gokji (LI 11) group were significantly decreased in all areas of cerebral cortex as compared to the Joksamni (ST 36) + Eumneungcheon (SP 9) group.
The optical densities of NPY positive neurons of the Joksamni (ST 36) + Gokji (LI 11) group were signifi-cantly decreased in primary motor cortex, primary somatosensory cortex, cingulate cortex as compared to the Joksamni (ST 36) and Joksamni (ST 36) + Eumneungcheon (SP 9) groups.
The optical densities of VIP positive neurons of the Joksamni (ST 36) + Eumneungcheon (SP 9) group were significantly increased in all areas of cerebral cortex except f or cingulate cortex as compared to the Joksamni (ST 36) group. The optical densities of VIP positive neurons of the Joksamni (ST 36) + Gokji (LI 11) group were significantly decreased in auditory cortex, cingulate cortex, perirhinal cortex as compared to the Joksamni (ST 36) + Gokji (LIII) group were significantly decreased in all areas of cerebral cortex as compared to the Joksamni (ST 36) + Eumneungcheon (SP 9) group.
[Conclusions] The result demonstrated that electroacupuncture on Joksamni (ST 36) and its combination change the activities of the NO system and peptidergic system in the cerebral cortex of SHR and that acupoint combination is one of the important parameters for the effects.

No abstract available.
Mortality*

To evaluate the relationships among the c-erbB-2 oncogene expression, DNA ploidy and other prognostic factors, an immunohistochemical study of the c-erbB-2 oncogene product and flow cytometric analysis of DNA ploidy were performed in paraffin sections of 42 cases of ovarian carcinomas. The results were as follows: 1) The positive reaction for c-erbB-2 oncogene product was observed mainly along the cytoplasmic membrane, and occasionally within the cytoplasm of the tumor cells. 2) Overall the positivity of c-erbB-2 oncogene expression was 45.2% of the ovarian carcinomas. By the histological types, the positivity was 35.7% in serous carcinoma, 80.0% in mucinous carcinoma, and 45.2% in endometrioid carcinoma; by the degree of differentiation, 57.1% in well differentiated carcinoma, 40.0% in moderately differentiated, and 27.3% in poorly differentiated; by the nuclear grading, 58.3% in grade I, 52.6% in grade II, and 18.2 % in grade III; and by the clinical staging, 57.1% in stage I, 42.8% in stage II, and 35.0% in stage III. The expression of the c-erbB-2 oncogene in the ovarian carcinomas was higher in the tumors of good differentiation, of the lower nuclear grade and of the lower clinical stage. 3) The incidence of DNA aneuploidy in the cases positive for the c-erbB-2 oncogene expression(47.3%) was higher than that in the negative cases(31.4%). From the above results, therefore, it is suggested that the c-erbB-2 oncogene may be involved in the early stage of ovarian carcinogenesis. Also suggested is that ovarian carcinomas positive for the c-erbB-2 oncogene in the early stages may have higher probability of having a DNA aneuploid cell line during the progress of the tumors.
Adenocarcinoma, Mucinous ; Aneuploidy ; Carcinogenesis ; Carcinoma, Endometrioid ; Cell Membrane ; Cytoplasm ; DNA* ; Incidence ; Oncogene Proteins ; Oncogenes ; Paraffin ; Ploidies*

Dermatitis herpetiformis is a chronic, intensely itchy, papulovesicular skin disorder of unknown cause, which is usually symmetrically distributed on extensor surface. Most patients have an associated gluten-sensitive enteropathy which is usually asymptornatic. We report six cases of dermatitis herpetiformis diagnosed by direct immunofluorescence studies. The patients have had characteristic features of dennatitis herpetiformis, such as vesicles, erythernatous papules, urticaria-like plaques, and hypopigmentation or hyperpigmentation, dispersed on the neck, back, and arms. None of the patients had subjective symptoms associated with gluten-sensitive enteropathy. Clinicians should be aware of the clinical features of this disease to avoid possible misdiagnosis, and to provide better therspeutic approaches in time.
Arm ; Celiac Disease ; Dermatitis Herpetiformis* ; Dermatitis* ; Diagnostic Errors ; Fluorescent Antibody Technique, Direct ; Humans ; Hyperpigmentation ; Hypopigmentation ; Neck ; Skin