1.A Study on the Expression of p53 Oncogene Products, PCNA Index and DNA Ploidy in Renal Cell Carcinoma.
Jong Jae JUNG ; Ji Shin LEE ; Chan CHOI
Korean Journal of Pathology 1997;31(7):672-682
Mutant p53 is associated with the advanced stages of some human tumor but there is a wide variation in the reported incidence of p53 mutation in renal cell carcinoma and its prognostic significances. We designed this study to assess the expression of p53 in renal cell carcinomas and to compare with the established prognostic factors. Immunoreactivity for p53 protein and proliferating cell nuclear antigen (PCNA) were assessed in 44 cases of primary renal cell carcinoma, and flow cytometric analysis of DNA ploidy was perfon-ned in 37 of those cases. p53 protein was over-expressed in 16/44 (36.4%) renal cell carcinomas and 5 rumors had more than 10 immunoreactive tumor cells. The expression of p53 protein was positively related to nuclear grade (p=0.007) and PCNA index (p=0.002), but was independent of stage and DNA ploidy. In univariate survival analysis, stage (p<0.001), nuclear grade (p=0.017), DNA ploidy (p=0.045) and PCNA index (p<0.001) were significantly associated with patient survival. However, considering the stage, all of the last three factors had no prognostic influence. Cases showing strong positivity of p53 expression had worse prognosis than those with no or weak p53 expression, especially in early lesions (stage I,II) (p<0.001).
Carcinoma, Renal Cell*
;
DNA*
;
Humans
;
Incidence
;
Oncogene Proteins*
;
Oncogenes*
;
Ploidies*
;
Prognosis
;
Proliferating Cell Nuclear Antigen*
2.Malignant Granular Cell Tumor of the Shoulder: A case report.
Jae Hun CHUNG ; Jae Hyuk LEE ; Jong Hee NAM ; Chan CHOI ; Min Cheol LEE
Korean Journal of Pathology 2000;34(6):475-479
A malignant granular cell tumor (MGCT) occurred in the left shoulder of a 62-year-old man. The patient underwent wide marginal excision followed by chemotherapy and radiotherapy. A metastatic tumor was identified in the axillary lymph node 22 months after the excision of the shoulder mass. The primary tumor was a poorly circumscribed mass measuring 5 5 4 cm. On cut section, it was a solid mass with yellowish tan color. Histologically, both primary and metastatic tumor consisted of polygonal cells with abundant granular cytoplasm and a vesicular nucleus with a prominent nucleolus. Two to three mitotic figures per ten high power fields at 200 were counted. Tumor cells were weakly stained with periodic acid-Schiff (PAS) preparation both before and after diastase digestion, and were positive for S-100 protein, neuron-specific enolase (NSE), and vimentin. By electron microscopy, the cytoplasm was filled with numerous autophagolysosomes containing myelin figures, mitochondria, and fragmented rough endoplasmic reticula. Basal laminae and angulated bodies were also noted. These findings suggest schwannian differentiation of this tumor.
Amylases
;
Basement Membrane
;
Cytoplasm
;
Digestion
;
Drug Therapy
;
Granular Cell Tumor*
;
Humans
;
Immunohistochemistry
;
Lymph Nodes
;
Microscopy, Electron
;
Middle Aged
;
Mitochondria
;
Myelin Sheath
;
Phosphopyruvate Hydratase
;
Radiotherapy
;
S100 Proteins
;
Shoulder*
;
Triacetoneamine-N-Oxyl
;
Vimentin
3.Congenital aortopulmonary fistula presenting as an exertional dyspnea.
Tae Hun KIM ; Chan Il MOON ; Jae Woong CHOI ; Myung Ju CHOI
Korean Circulation Journal 2000;30(10):1291-1294
Aortopulmonary fistula is an exceedingly rare vascular malformation. It is commonly derived after chest injuly or from complication of chest operation and aortic dissection and congenital aortopulmonary fistula is only several cases combined with Tetralogy of Fallot or aortic stenosis. But a congenital aortopulmonary fistula without any hemodynamic abnormalities was not reported. A 56-year old man with exertional dyspnea was admitted. In an examination on admission, there were no abnomalities. Aortography showed an aortopulmonary fistula that branches from the ascending aorta adjacent to the right coronary artery, running to the main pulmonary artery. Transcatheter coil embolization was performed and he was discharged 7 day after embolization without complication. Exertional dyspnea disappeared and careful follow up has be performed periodically.
Aorta
;
Aortic Valve Stenosis
;
Aortography
;
Coronary Vessels
;
Dyspnea*
;
Embolization, Therapeutic
;
Fistula*
;
Follow-Up Studies
;
Hemodynamics
;
Humans
;
Middle Aged
;
Pulmonary Artery
;
Running
;
Tetralogy of Fallot
;
Thorax
;
Vascular Malformations
4.Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR.
Dong Kyun SUH ; Suk Kyung LYM ; You Chan BAE ; Keun Woo LEE ; Won Pil CHOI ; Jae Chan SONG
Journal of Veterinary Science 2000;1(1):33-37
Lawsonia intracellularis is not culturable with a standard bacteriologic culture. One step PCR assay as a clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis (PPE) caused by L. intracellularis. Primers were designed based on the p78 DNA clone of L. intracellularis. The one step PCR resulted in the formation of a specific 210-bp DNA product derived from L. intracellularis. The nonspecific amplification product was not detected with swine genomic DNA or other bacterial strains causing similar symptoms to L. intracellularis infection. The one step PCR was as sensitive as 100 pg of L. intracellularis genomic DNA. We applied this method to field specimens diagnosed as PPE by macroscopic observation. Of 17 mucosal scraping specimens, 16(94%) were identified as positive to PPE and 15(88%) of 17 feces specimens. These results suggest that the one step PCR can be used as a rapid diagnostic method for L. intracellularis infection.
Animals
;
Base Sequence
;
DNA Primers
;
Desulfovibrionaceae Infections/diagnosis/*veterinary
;
Ileum/microbiology/pathology
;
Intestinal Mucosa/microbiology/pathology
;
Lawsonia Bacteria/genetics/*isolation & purification
;
Polymerase Chain Reaction/*methods
;
Reproducibility of Results
;
Sensitivity and Specificity
;
Swine
;
Swine Diseases/*diagnosis/microbiology
5.The Clinical Efficacy of Amniotic Membrane Transplantation and Limbal-Conjunctival Autograft in Patients with Recurrent Pterygium or Pseudopterygium.
Gun Sic PARK ; Tae Hoon CHOI ; Woo Chan PARK ; Jae Chan KIM
Journal of the Korean Ophthalmological Society 2001;42(8):1143-1149
PURPOSE: To evaluate the clinical efficacy of amniotic membrane transplantation and limbal-conjunctival autograft for patients with recurrent pterygium or pseudopterygium, the results of 15 eyes of 14 patients were analyzed. METHODS: Total 10 cases of recurrent pterygium and 5 cases of pseudopterygium were surgically managed during June 1998 to July 2000. After excision of pterygium, amniotic membrane was attached on the bare sclera and limbal-conjunctival autograft was transplanted over the amniotic membrane. Mean follow-up period was 47.2 week and all patients were examined for recurrence, which was graded from G0(no vessel) to G3(fibrotic tissue invading the limbus). RESULTS: 12 out of 15 cases(rate 80%) showed no recurrence(grade 0). 1 case of grade 2 recurrence and 2 cases of grade 3(total 3 cases) were observed(rate 20%). Reoperation was performed on 2 cases of grade 3 recurrence. There was no further recurrence necessitating additional reoperation. 1 case of limbal graft was detached and removed. The operation time of this more prolonged than the other procedures, but there were no significant complications. CONCLUSION: Amniotic membrane and limbal-conjunctival autograft for patients with recurrent pterygium or pseudopterygium reconstructed complete ocular surface with less recurrence. Therefore, we recommend this surgical approach as a safe and effective method for the treatment of complicated cases of pterygium.
Amnion*
;
Autografts*
;
Follow-Up Studies
;
Humans
;
Pterygium*
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Recurrence
;
Reoperation
;
Sclera
;
Transplants
6.Syphilitio abdominal aortic aneurysm associated with deep vein thrombosis and pulmonary embolism.
Hee Jae JUN ; Pill Jo CHOI ; Si Young HAM ; Si Chan SUNG ; Jong Su WOO
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(10):1141-1145
No abstract available.
Aortic Aneurysm, Abdominal*
;
Pulmonary Embolism*
;
Venous Thrombosis*
7.Effect of GnRH Agonist in the Treatment of Uterine Myoma.
Heung Tae HOH ; Sang hyuk LIM ; Jae Sung CHOI ; Chan Ho SONG
Korean Journal of Gynecologic Oncology and Colposcopy 1996;7(4):270-277
Fifteen women with symptomatic uterine myomas, diagnosed by clinical examination and confirmed by pelvic ultrasonography, were treated with intranasal insufflation of Nafarelin acetate, 200 micrograms, twice a day, for a total treatment period of 6 months. Treatment was evaluated with respect to subjective symptoms, changes in myoma size and uterine volume, variations in blood estradiol, FSH, LH and CA 125, and side effects. The following results were obtained: 1. All patients showed a marked reduction(p<0.001) in uterine volume. Before treatment volume measured 312.3+/-24.2cm3, after 12 weeks volume was 132.4+/-40.6cm3, and after 24 weeks it was 123.6+/-48.3cm3. 2. Blood estradiol and LH levels were decreased significantly(p<0.05) after treatment. The FSH level was decreased, but not significantly. 3. Blood CA 125 levels were increased 6 cases(40.0%) before treatment. The levels were normalized in all 6 cases after treatment. 4. Symptoms of uterine myoma disappeared or decreased. 5. Minor side effects, such as hot flushes, headache, general myalgia and fatigue, and vaginal dryness were encountered frequently although none necessitating discontinuation of treatment, These data suggest that Nafarelin acetate is useful for the treatment of uterine myoma. However, appropriate indications should be selected in the treatment of uterine myoma because the possible regrowth of uterine myoma after treatment limits the use of GnRH agonist.
Estradiol
;
Fatigue
;
Female
;
Gonadotropin-Releasing Hormone*
;
Headache
;
Humans
;
Insufflation
;
Leiomyoma*
;
Myalgia
;
Myoma
;
Nafarelin
;
Ultrasonography
8.Long or Multiple Stenting in Primary Angioplasty.
Jae Woong CHOI ; Chan Il MOON ; Chang Sup SONG ; Kyong Tae JEONG ; Soon Chang PARK
Korean Circulation Journal 1999;29(12):1341-1349
BACKGROUND: Primary stenting as a direct reperfusion procedure after acute myocardial infarction might reduce recurrent myocardial infarction and target vessel revascularization. However, result of long or multiple stenting in the long or tandem lesions were not known. METHOD: From Jan. 1996 to Dec. 1998, patients with acute myocardial infarction including cardiogenic shock were undergone primary stenting using long or multiple stent. The clinical end points were death, recurrent infarction, subsequent bypass surgery, or repeat angioplasty of the infarct-related vessel. The results were compared with age, sex, lesion, and risk matched standard stenting group. RESULT: Baseline characteristics were similar for the 20 patients who underwent standard length stenting and the 13 patients who underwent long or multiple stenting. Stent diameter was similar in two group (3.4+/-0.3 mm vs. 3.5+/-0.4 mm, p=0.65), but total stent length was longer in long or multiple stenting group (15.5+/-1.8 mm vs. 40.18.4 mm, p=0.01). Average numbers of stent using in multiple stenting were 1.5+/-0.7. Stenting in the infarct-related artery was successful in all patients randomized to stent treatment. At 6 months, the incidence of the primary end point was 25% (5/20) in the standard stent group and 31%(4/13) in the long or multiple stent group (p=0.5). CONCLUSION: Outcomes of long or multiple stenting including mortality, recurrent myocardial infarction and target vessel revascularization was similar to standard stenting. Long or multiple stenting after acute myocardial infarction may possible procedure in some selective cases having long or tandem lesion.
Angioplasty*
;
Arteries
;
Humans
;
Incidence
;
Infarction
;
Mortality
;
Myocardial Infarction
;
Reperfusion
;
Shock, Cardiogenic
;
Stents*
9.PCR Approach for Detection and Typing of Epidermodysplasia Verruciformis-associated Human Papillomavirus Types.
Yong Jae SHIN ; Hyun Chul CHOI ; Chan Gil KIM ; Kwang Ho LEE
Journal of the Korean Society of Virology 1999;29(1):39-44
Warts, or verrucae, are benign epithelial proliferations of the skin and mucosa caused by infection with human papillomaviruses (HPV). It is now recognized that there are many different HPV types. Especially type3 is most frequently observed in flat wart. Other types, such as type2, 10, 14, 27, 28, 29, 38, and 41 are rarely encounted in flat wart. We describe here a simple and economic method for detection and identification of epidermodysplasia verruciformis-associated HPV. The method is based on polymerase chain reaction (PCR) amplification and restriction analysis. The method has been developed with cloned HPV DNA and DNA from clinical samples. Clinical samples are from either frozen tissue or paraffin-embedded tissue. Genomic fragments were obtained from two different HPV types (3 and 10). The amplification fragments were identified by a form of miniature fingerprinting, with a set of restriction enzymes that gave a unique digestion pattern for each HPV type. We have tested 74 clinical samples. Only type3 among these clinical samples is detected, and one sample is involved in neither type3 nor type10.
Clone Cells
;
Dermatoglyphics
;
Digestion
;
DNA
;
Humans*
;
Mucous Membrane
;
Polymerase Chain Reaction*
;
Skin
;
Warts
10.Two cases of angioedema due to C1 inactivator deficiency.
Ji Young SUH ; Jae Kyung CHOI ; Ha Baik LEE ; Chan Keum PARK
Pediatric Allergy and Respiratory Disease 1993;3(1):121-127
No abstract available.
Angioedema*