In order to investigate the molecular basis of the aging process in brain, we have employed high-density oligonucleotide microarrays providing data on 10,108 gene clusters to define transcriptional patterns in three brain regions, cerebral cortex, cerebellum, and hippocampus. Comparison of the expression patterns between young (6-week-old) and aged (17-month-old) C57BL/6 male micerevealed that about ten percent (1098) of the genes showed a significant change in the expression level in at least one of the three tissues. Among them, 23 genes were upregulated and 62 genes were downregulated in all three tissues of the old mice. The number of genes upregulated exclusively in hippocampus (337) was much larger compared to other tissues. Gene ontology-based analysis showed the genes related with signal transduction or molecular transports are more likely to be upregulated than downregulated in the aging process of hippocampus. These data may provide some useful means for elucidating the molecular aspect of aging in hippocampus and other regions in brain.
Aging* ; Animals ; Brain* ; Cerebellum ; Cerebral Cortex ; DNA* ; Gene Expression* ; Hippocampus ; Humans ; Male ; Mice* ; Multigene Family ; Oligonucleotide Array Sequence Analysis* ; Signal Transduction ; Transcriptome*

PURPOSE: The aim of this article is to analyze the preference for treatment modality of dentists. MATERIAL AND METHODS: Data of 20,038 patients was involved. Data analysis were done by distribution according to the various kinds of prosthesis, including complete denture, removable partial denture, fixed partial denture, implant-supported dental prosthesis as well as distribution according to the professional titles of the dentists finishing the treatment, including resident and professors. RESULTS: The number of cases of dental prosthesis increased year by year. 61.06% of the patients accepted fixed partial denture restoration. The number of patients who accepted implant supported restoration is also increasing year by year. The number of complete denture, implant-supported dental prosthesis finished by professors was larger than that done by residents, while it was contrary for removable partial denture, fixed partial denture, and the difference was statistically significant (P<.05). CONCLUSION: Professors and residents have some difference in the categorization of prosthesis finished. Fixed partial denture and implant-supported dental prosthesis are preferred.
Dental Prosthesis ; Dental Prosthesis, Implant-Supported ; Dentists ; Denture, Complete ; Denture, Partial, Fixed ; Denture, Partial, Removable ; Humans ; Prostheses and Implants ; Prosthodontics ; Statistics as Topic

Gastrointestinal complications, especially duodenal complication after cardiopulmonary bypass are rare, but often fatal. We experienced 1 case of duodenal ulcer bleeding and 2 cases of duodenal ulcer perforation developing after cardiopulmonary bypass from August 1994 to April 1996. In the case of duodenal ulcer bleeding, palpitation, dizziness, tachycardia and melena were the clues leading to diagnosis, and in the cases of perforation, abdominal distension with pain, tachycardia, hypotension, oliguria were the clues. Duodenal perforations were diagnosed by abdominal paracentesis. The patient with duodenal bleeding was treated by H-2 receptor antagonist, antacids and transfusion. And emergency laparotomy was required for the patients with duodenal perforation. In addition to ulcer prophylaxis including H-2 receptor antagonist and antacids, a high index of suspicion and timely surgery are necessary for early diagnosis and appropriate treatment of duodenal complication developing after cardiopulmonary bypass.
Antacids ; Cardiopulmonary Bypass ; Diagnosis ; Dizziness ; Duodenal Ulcer ; Early Diagnosis ; Emergencies ; Heart* ; Hemorrhage ; Humans ; Hypotension ; Laparotomy ; Melena ; Oliguria ; Paracentesis ; Postoperative Complications ; Tachycardia ; Thoracic Surgery* ; Ulcer

BACKGROUND AND OBJECTIVES: Organizing hematoma of the maxillary sinus can be developed by organizing procedure of hematoma in the unaerated maxillary sinus. To our knowledge, this is the second article describing organizing hematoma in the maxillary sinus. We experienced 7 cases recently, and aimed to describe clinical features, radiologic characteristics, and pathological findings. MATERIALS AND METHODS: Seven patients were diagnosed as organizing hematoma pathologically. We reviewed computed tomography (CT) and medical records to find out clinical features of organizing hematoma of the maxillary sinus. Of seven patients, five were male and two were female. Their mean age was 49 years. RESULTS: All patients had a history of frequent epistaxis without bleeding tendency. They also complained of nasal obstruction, hyposmia, headache, and cheek swelling. Rhinoscopy revealed obliterated nasal cavity due to bulging lateral wall. CT scans showed soft-tissue densities expanding the maxillary sinus with the minimal contrast enhancement. Destruction of the bony wall was observed in two patients. Endoscopic sinus surgery, Caldwell-Luc's operation, and Denker's operation were applied for the treatment singly or in combination, and resulted in successful outcome. CONCLUSION: Organizing hematoma should be included in the differential diagnosis when patients have frequent epistaxis and expansile lesion in the maxillary sinus.
Cheek ; Diagnosis, Differential ; Epistaxis ; Female ; Headache ; Hematoma* ; Hemorrhage ; Humans ; Male ; Maxillary Sinus* ; Medical Records ; Nasal Cavity ; Nasal Obstruction ; Tomography, X-Ray Computed

A 42-year-old male was referred to our pain clinic with back pain. Four years ago, he was treated for back pain caused by falling down injury. He also complained of erectile dysfunction, which was regarded as having psychologic origin at other departments. He had grade IV leg weakness and left lower thoracic paravertebral tenderness. Thoracic CT, plane X-ray and whole body bone scan revealed normal findings. After diagnostic medial branch block at lower thoracic vertebra, pain was subsided partially, but erectile dysfunction persisted. Due to persistent symptoms, we ordered MRI, and the extramedullary mass at T9 level was found. He had undergone neurosurgical operation for tumor removal, and pathologic finding was Schwannoma. After the operation his symptoms improved completely. Although erectile dysfunctions are presumed to have psychologic origin frequently, we report a patient whose symptoms were from the spinal cord tumor.
Adult ; Back Pain ; Erectile Dysfunction ; Humans ; Leg ; Male ; Neurilemmoma ; Pain Clinics ; Spinal Cord Neoplasms ; Spine

The term myotubular myopathy (MTM) implies a maturational arrest of fetal muscle during the myotubular stage of development at 8-15 weeks of gestation. Characteristic muscle histopathology consists of small hypotrophic muscle fibers with centrally placed nuclei and a surrounding clear area devoid of myofibrils. X-linked recessive inheritance is the most common trait. Autosomal recessive and autosomal dominant forms are less frequently reported. The clinical diagnostic criterion for X-linked MTM has relied on a positive family history and the demonstration of the presence of characteristic biopsy findings from affected male subjects. Additional features may include perinatal onset, severe hypotonia, respiratory failure, dysphagia, thin ribs, contractures of the hips or knees, puffy eyelids and ophthalmoplegia. The prognosis is often fatal, and most patients die within the first year of life from respiratory failure. The authors report a case of presumed X-linked MTM with severe hypotonia, muscle weakness and respiratory failure at birth.
Biopsy ; Contracture ; Deglutition Disorders ; Eyelids ; Hip ; Humans ; Knee ; Male ; Muscle Hypotonia ; Myofibrils ; Myopathies, Structural, Congenital* ; Ophthalmoplegia ; Parturition ; Pregnancy ; Prognosis ; Respiratory Insufficiency ; Ribs ; Wills

BACKGROUND AND OBJECTIVES: Overcorrection is defined as obvious deviation of the septum to the opposite direction after septoplasty and it is not infrequently developed in young patients. We investigated the rate of its incidence, especially in relation to age. MATERIALS AND METHODS: We retrospectively studied 1,124 patients undergoing septoplasty operation for septal deviation between 1994 and 1999. The operations and postoperative observation were performed by a single surgeon. We reviewed the medical records for age, sex, symptoms, combined operations, direction of deviation before and after septoplasty. RESULTS: Out of 1,124 patients, 22 (2.0%) had their septum overcorrected after septoplasty and 21 (95%) complained of nasal obstruction of the newly deviated side. The incidence of overcorrection was 7.3% (16/218) in teens, 1.7% (5/294) in twenties, 0.4% (1/250) in thirties, and 0% in older ages. The incidence in teens and early twenties was 5%. Patients of teenagers revealed significantly higher incidence as compared to other age groups (p<0.05). CONCLUSION: Overcorrected septum as a complication of septoplasty develops at the incidence rate of 5% in patients of teens and early twenties. Authors suggest that the depth of cross-hatching incision on the cartilage should be superficial or half-thickness to avoid overcorrection in young patients. And the possibility of overcorrection or revision septoplasty should be informed preoperatively.
Adolescent ; Cartilage ; Humans ; Incidence ; Medical Records ; Nasal Obstruction ; Retrospective Studies

PURPOSE: Artificial intelligence (AI), represented by deep learning, can be used for real-life problems and is applied across all sectors of society including medical and dental field. The purpose of this study is to review articles about deep learning that were applied to the field of oral and maxillofacial radiology. MATERIALS AND METHODS: A systematic review was performed using Pubmed, Scopus, and IEEE explore databases to identify articles using deep learning in English literature. The variables from 25 articles included network architecture, number of training data, evaluation result, pros and cons, study object and imaging modality. RESULTS: Convolutional Neural network (CNN) was used as a main network component. The number of published paper and training datasets tended to increase, dealing with various field of dentistry. CONCLUSION: Dental public datasets need to be constructed and data standardization is necessary for clinical application of deep learning in dental field.
Artificial Intelligence ; Dataset ; Dentistry ; Learning

Purpose: As cone-beam computed tomography (CBCT) has become the most widely used 3-dimensional (3D) imaging modality in the dental field, storage space and costs for large-capacity data have become an important issue. Therefore, if 3D data can be stored at a clinically acceptable compression rate, the burden in terms of storage space and cost can be reduced and data can be managed more efficiently. In this study, a deep learning network for super-resolution was tested to restore compressed virtual CBCT images. Materials and Methods: Virtual CBCT image data were created with a publicly available online dataset (CQ500) of multidetector computed tomography images using CBCT reconstruction software (TIGRE). A very deep superresolution (VDSR) network was trained to restore high-resolution virtual CBCT images from the low-resolution virtual CBCT images. Results: The images reconstructed by VDSR showed better image quality than bicubic interpolation in restored images at various scale ratios. The highest scale ratio with clinically acceptable reconstruction accuracy using VDSR was 2.1. Conclusion VDSR showed promising restoration accuracy in this study. In the future, it will be necessary to experiment with new deep learning algorithms and large-scale data for clinical application of this technology.

The inducible 70 kDa heat shock proteins (Hsp70) in mice are encoded by two almost identical genes, hsp70.1 and hsp70.3. Studies have found that only hsp70.1 is induced by hypertonic stress while both hsp70.1 and hsp70.3 genes are expressed in response to heat shock stress. It is unclear if the human counterparts, hsp70-2 and hsp70-1, are differentially regulated by heat shock and osmotic stress. This study found that only hsp70-2 was induced by hypertonic stress in human embryonic kidney epithelial cells and fibroblasts, while heat shock stress induced both hsp70-1 and hsp70-2. The human hsp70-2 promoter region contains three TonE (tonicity-responsive enhancer) sites, which were reported to play an important role in the response to hypertonicity. When the reporter plasmids containing different parts of the 5' flanking region of hsp70-2 were transfected into human embryonic kidney epithelial cells or fibroblasts, one TonE site at -135 was found to play a key role in the response to hypertonicity. The inactivation of the TonE site using site-directed mutagenesis led to the complete loss of induction by hypertonicity, which demonstrates the essential role of the TonE site. This suggests that the TonE site and the TonEBP (TonE binding protein) are the major regulators for the cellular response against high osmolarity in human kidney tissue.
Transcription, Genetic/drug effects/genetics ; Transcription Factors/genetics/*physiology ; Saline Solution, Hypertonic/*pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; Protein Binding ; Promoter Regions (Genetics)/genetics ; Point Mutation ; Mutagenesis, Site-Directed ; Humans ; HSP70 Heat-Shock Proteins/*genetics/metabolism ; Gene Expression Regulation/*drug effects ; DNA-Binding Proteins/genetics/metabolism ; Cell Line ; Binding Sites/genetics ; Base Sequence ; 5' Flanking Region/genetics