1.Histopathologic Analysis of Helicobacter pylori-associated Chronic Gastritis between cagA-positive and cagA-negative Strains.
Hun Kyung LEE ; Gyeong Hoon KANG ; Hwoon Yong JUNG ; On Ja KIM
Korean Journal of Pathology 1998;32(7):504-510
Infection with Helicobacter pylori (H. pylori) leads to gastritis, but the majority of infected persons are asymptomatic, and it has been recently described that the ability of H. pylori to cause more severe disease is related to the presence of the cytotoxin-associated gene A (cagA). We investigated the prevalence of cagA-bearing strains in a group of H. pylori-positive gastritis, and compared the morphologic differences between cagA-positive and cagA-negative cases on H&E stained slides. Polymerase chain reaction (PCR) assays for detection of H. pylori and cagA of 62 gastric biopsy specimens were performed. All the slides were analyzed by the updated Sydney system. Forty eight (77.4%) were PCR positive for H. pylori and thirty four (54.8%) were positive for cagA. There were no significant differences in numbers of H. pylori, degree of infiltration of mononuclear cells and degree of atrophy between cagA-positive and cagA-negative groups. The rates of neutrophilic infiltration and intestinal metaplasia were significantly higher in cagA-positive group than in cagA-negative group. In conclusion, the detection of H. pylori by PCR method is more sensitive than that of microscopic examination and H. pylori strains possessing cagA are associated with an enhanced induction of severe gastritis.
Atrophy
;
Biopsy
;
Gastritis*
;
Helicobacter pylori
;
Helicobacter*
;
Humans
;
Metaplasia
;
Neutrophils
;
Polymerase Chain Reaction
;
Prevalence
2.Effect of Integrated Personalized Health Care System on Middle-Aged and Elderly Women's Health.
Hiye Ja LEE ; Kyung Ja KANG ; Seung Hun PARK ; Se Jin JU ; Mi Hwa JIN ; Boc Nam PARK
Healthcare Informatics Research 2012;18(3):199-207
OBJECTIVES: Body weight, body mass index (BMI), body fat, and blood pressure are important indicators of a person's health. In this experimental study, we evaluated the effectiveness of an integrated personalized health care system, Health Improvement and Management System (HIMS)-everyday, which instantly provides subjects with biofeedback on their measured body weight, BMI, body fat and blood pressure using a database that stores subjects-customized information. METHODS: The subjects of this study used the system once or twice a week for 8 weeks. We analyzed the changes in their body weight, BMI, body fat, and blood pressure according to their respective usage of the system, and analyzed the changes in their perceived health status and health promoting behavior accordingly. RESULTS: Subjects' body weight, BMI, and blood pressure decreased significantly with respect to their individual usage of the system. Subjects who used the system more frequently showed significant improvement in their body weight, BMI, and body fat. However, subjects' perceived health status and health promoting behavior did not improve significantly. CONCLUSIONS: The study showed that the biofeedback-based personalized health care system was effective in controlling middle-aged and elderly women' body weight, BMI, body fat, and blood pressure.
Adipose Tissue
;
Aged
;
Biofeedback, Psychology
;
Blood Pressure
;
Body Mass Index
;
Body Weight
;
Delivery of Health Care
;
Health Behavior
;
Humans
;
Information Systems
;
Women's Health
3.Thin-Section CT with Air Insufflation Technique for Bladder Carcinoma: CT Findings of Superficial Bladder Carcinoma.
Mi Hye KIM ; Kyung Sub SHINN ; Hyun KIM ; Ha Hun SONG ; Si Won KANG ; Eun Ja LEE ; Young Sin KIM
Journal of the Korean Radiological Society 1994;30(2):347-351
PURPOSE: The staging of bladder carcinoma is a major determinant of operative management. CT of bladder carcinoma has been widely used to diagnose external extension (pT3b and over), but tumors confined to the bladder wall (from pT1 to pT3a) are poorly delineated. The authors describe CT findings of the superficial bladder carcinoma (below T1, stage A) in thin section CT with air insufflation technique (air insufflation-CT) to facilitate early detection and to aid correct staging of the superficial bladder carcinoma. MATERIALS AND METHODS: The materials consisted of proved 24 cases (1'9 patients, single tumor: 16 patients, multiple tumors :3 patients) of stage A bladder carcinomas. Air insufflation-CT was performed by the infusion of approximately 200 mL of air into the bladder via a Foley catheter. After the routine pelvic CT, bladder tumors were re-scanned with 1.5 to 5 mm thickness and intervals. RESULTS: The superficial bladder carcinomas were detected as nodular(5 cases, 20.8%), papillary(15 cases, 62.5%), pyramidal(2 cases, 8.3%), and domed(2 cases, 8.3%) forms on air insufflation CT. These tumors were classified into three types according to the size of the tumoral neck:type I(pedundulated polypold tumor:4 cases, 16.6%), type II(polypid tumor with short neck :13 cases, 54.2%), and type Ill(sessile tumor :7 cases, 29. 2%). The mean size(tumoral width x height x base o, neck/stalk) of the tumors was 22 x 20 x 16mm. The average tumoral sizes according to each type of the superficial tumors were type 1:22 x 25 x 6mm, type 11:23 x 22 x 18mm, and type III :18 x 15 x 18mm. The mean width of the type I--II tumoral necks was 15mm. The mean length of the type I tumoral neck(pedicle) was 2.5mm. Papillary fronds of the tumors were seen in 10 cases(41.7%) of 24 superficial tumors. Outer margin of the involved bladder wall was smooth in all cases. CONCLUSION: Thin-sectin CT with air insufflation technique for bladder carcinoma was useful in tumoral demonstration, and characteristics of the superficial bladder carcinomas were small polypold tumors had a short neck mostly and smooth outer wall of the involved bladder wall.
Catheters
;
Humans
;
Insufflation*
;
Neck
;
Urinary Bladder Neoplasms
;
Urinary Bladder*
4.Changes in the Cornea and Anterior Chamber After LASEK: Pentacam Findings.
Jin Hyoung PARK ; Ja Hun KANG ; Kyung Hyun JIN
Journal of the Korean Ophthalmological Society 2009;50(4):510-517
PURPOSE: We evaluated changes in the cornea and anterior chamber after LASEK using Pentacam to search for signs of subclinical keratectasia occurrence. METHODS: Seventy-one eyes of 36 patients who had received LASEK were enrolled in this study. All eyes were examined for asphericity of the anterior and posterior cornea (Q-value), anterior and posterior corneal displacement, central corneal thickness, anterior chamber depth, angle, and volume using Pentacam before surgery and again one month after the operation. RESULTS: The postoperative changes in the asphericity of the posterior cornea and posterior corneal displacement were not statistically significant(p=0.668, p=0.101). The anterior chamber depth, angle, and volume decreased by 0.088 mm, 0.983degrees, 7.21 mm3 after LASEK, respectively. (p=0.000) The postoperative changes in asphericity of the anterior cornea, anterior corneal displacement, and central corneal thickness were statistically significant(p=0.000). CONCLUSIONS: In this study, we compared changes in the anterior chamber and cornea after LASEK using Pentacam. Changes in the anterior cornea were significant; however, the posterior cornea did not change significantly. The anterior chamber depth, anterior chamber angle, and volume were decreased one month after the operation, although further long-term follow-ups will be necessary to verify these findings.
Anterior Chamber
;
Cornea
;
Displacement (Psychology)
;
Eye
;
Follow-Up Studies
;
Humans
;
Keratectomy, Subepithelial, Laser-Assisted
5.c-kitMutation and Immunohistochemical Expression in Gastrointestinal Stromal Tumors.
Dong Wook KANG ; Joo Heon KIM ; Dong Hun KIM ; Kung Hee KIM ; Mee Ja PARK ; Dae Young KANG
Korean Journal of Pathology 2003;37(4):246-254
BACKGROUND: Gastrointestinal stromal tumor (GIST) is the most common non-epithelial neoplasm arising in the gastrointestinal tract. The aim of this study is to investigate the correlation among the clinicopathologic features, presence of c-kit mutation, and immunohistochemical expression of c-kit in 61 cases of GISTs. METHODS: We divided the GISTs into three groups as benign, boderline and malignant, according to histologic grade. Exon 11 of the c-kit was amplified by PCR and sequenced. We performed immunohistochemical study for CD117, CD34, vimentin, SMA, desmin, and S-100 protein. RESULTS: Twenty-one cases were diagnosed as benign GISTs, 14 cases as borderline GISTs, and 26 cases as malignant GISTs. The shape, atypia, cellularity, and necrosis showed good correlations with the histologic grades of the GISTs.Mutations of exon 11 of the c-kit were detected in 3 benign GISTs, 4 borderline GISTs, and 13(%) malignant GISTs. Sequence analysis confirmed the deletion mutation (n=16) and the singlebase pair mutation (n=4). The immunohistochemical stainings showed myogenic differentiation(n=20), neurogenic differentiation (n=15), and neither myogenic or neurogenic differentiation(n=34). CONCLUSIONS: The GIST is the primitive mesenchymal tumor capable of divergent differentiation, and the mutation of the c-kit is a good parameter for the malignant GIST.
Desmin
;
Exons
;
Gastrointestinal Stromal Tumors*
;
Gastrointestinal Tract
;
Immunohistochemistry
;
Necrosis
;
Polymerase Chain Reaction
;
S100 Proteins
;
Sequence Analysis
;
Sequence Deletion
;
Vimentin
6.Metastatic Thy roid Carcinoma of Jugular Foramen Simulating Glomus Jugulare Tumor: A Case Report.
Eun Ja LEE ; Dong Hun YANG ; Chul Ku JUNG ; Si Won KANG
Journal of the Korean Radiological Society 1999;41(6):1097-1100
We report a case of hyper vascular metastatic thyroid carcinoma of the jugular fora-men simulating a glomus jugulare tumor. Computed tomography(CT) revealed areas of irregular lytic bony destruction of the left jugular foramen, as well as characteristic in vasion routes of a glomus jugulare tumor. Magnetic resonance(MR) imaging and angiography demonstrated a hypervascular mass similar to a glomus tumor.
Angiography
;
Glomus Jugulare Tumor*
;
Glomus Jugulare*
;
Glomus Tumor
;
Thyroid Neoplasms
7.Immunophenotyping and DNA analysis of lymphoid leukemic cells by FACScan system.
Young Ho HAHM ; Joung A KANG ; Kyung Eun SONG ; Jang Soo SUH ; Won Kil LEE ; Jay Sik KIM ; Kun Soo LEE ; Ja Hun KOO
Korean Journal of Clinical Pathology 1992;12(4):445-455
No abstract available.
DNA*
;
Immunophenotyping*
8.Isolation and Characterization of Avian Metapneumovirus from Broiler Breeder Chickens in Korea.
Kang Seuk CHOI ; Woo Jin JEON ; Mi Ja PARK ; Eun Kyoung LEE ; Jun Hun KWON
Journal of Bacteriology and Virology 2009;39(4):373-382
Avian metapneumovirus (AMPV) is an emerging pathogen causing respiratory and reproductive illness in poultry worldwide. To demonstrate the presence of AMPV in domestic chickens in Korea, we attempted to isolate AMPV from affected chickens. A cytopathic agent was isolated using chicken tracheal ring culture from dead chickens from a broiler breeder farm with reduced egg production in Korea. This agent, termed SC1509 strain, subsequently passed in Vero cells with distinct cytopathic effects. The SC1509 strain was confirmed as avian metapneumovirus (AMPV) using both RT-PCR test and monoclonal antibody-based immunofluorescence assay. Sequence analysis based on the G glycoprotein revealed that the SC1509 strain had 22.5 to 96.0% nucleotide sequence identity and 11.1 to 92.7% predicted amino acid sequence identity with previously published AMPV strains, particularly with the highest sequence homology (95.8 to 96% for nucleotides and 92.2 to 92.7% for amino acids) to European strains belonging to genotype B. The SC1509 strain was phylogenetically clustered with genotype B viruses, confirming that the SC1509 strain belongs to genotype B. This is the first report of genotype B avian metapneumovirus from chickens in Korea.
Amino Acid Sequence
;
Base Sequence
;
Chickens
;
Fluorescent Antibody Technique
;
Genotype
;
Glycoproteins
;
GTP-Binding Proteins
;
Herpesvirus 1, Cercopithecine
;
Korea
;
Metapneumovirus
;
Nucleotides
;
Ovum
;
Poultry
;
Sequence Analysis
;
Sequence Homology
;
Sprains and Strains
;
Vero Cells
9.Vascular Endothelial Growth Factor-C Expression in Primary and Metastatic Brain Tumor.
Ji Hun CHUNG ; Tae Sik KONG ; Myoung Jae KANG ; Dong Geun LEE ; Myoung Ja CHUNG
Journal of Korean Neurosurgical Society 2002;32(6):543-547
OBJECTIVE: Vascular endothelial growth factor(VEGF)-C is involved in lymphangiogenesis and spreading of cancer cells via lymphatic vessels. The aim of the present study is to investigate the relationship between the absence of cerebral lymphatic vessels and low metastatic rate of brain tumors. METHODS: Immunohistochemical stains were performed for VEGF-C and VEGF in surgically resected specimens from 57 patients with primary(38 cases, low grade : 10 cases, high grade : 28 cases) and metastatic(19 cases) brain tumor. RESULTS: The expression of VEGF-C was higher in metastatic carcinoma(68%) than in high-grade primary tumor(29%). There was no difference for VEGF expression between high grade brain tumor (71%) and metastatic carcinoma(58%). CONCLUSION: Low VEGF-C expression of primary brain tumors may play a role in low metastatic rate of brain tumors.
Brain Neoplasms*
;
Brain*
;
Coloring Agents
;
Humans
;
Lymphangiogenesis
;
Lymphatic Vessels
;
Vascular Endothelial Growth Factor A
;
Vascular Endothelial Growth Factor C*
10.Knowledge and Performance of Infection Control Guidelines.
Kyung Mi KIM ; Kyeong Sook CHA ; Ji Young LEE ; So Yeon YOO ; Ok Ja CHOI ; Sung Hun WIE ; Wan Shik SHIN ; Moon Won KANG
Korean Journal of Nosocomial Infection Control 2004;9(2):139-150
BACKGROUND: This study was purposed to identify the knowledge and performance level of the infection control guidelines and to improve their performance level of infection control for those nurses who care patients directly. METHODS: This study was based on the survey questionnaires about infection control guidelines with the total 626 nurses in Seoul and Kyunggi areas for the period between May 26, 2003 and July 14, 2003. RESULTS: The average knowledge level of infection control was 0.84 +/-0.07 (score range 0-1) and the average performance level of the infection control was 4.44+/-10.27 (score range 0-5). The correlation between knowledge and performance of the infection control showed positive correlation (r=.39; P<.001). The knowledge and performance level of the 'Prevention of the central catheter infection' were higher than other infection control guidelines. The performance levels of the 'Prevention of the surgical site infection' were lower than other infection control guidelines. The correlation between knowledge and performance of the 'Prevention of the nosocomial pneumonia' (r=.389; P<.001). 'Prevention of the urinary tract infection' (r=.325; P<.001), 'Prevention of the central catheter infection (r=.290; P<.001) and 'Intravascular infection control' (r=.446; P<.001) showed positive correlation. CONCLUSION: The study demonstrated that the nurses' knowledge and performance of infection control guidelines showed positive correlation. So the education on the infection control is required indispensably and it would contribute to reduce the hospital infection rate by performing the right treatment on the infection control with the basis of accurate knowledge.
Catheters
;
Cross Infection
;
Education
;
Gyeonggi-do
;
Humans
;
Infection Control*
;
Seoul
;
Urinary Tract
;
Surveys and Questionnaires