Background and objective Lymph node metastasis is one of the important factors affecting the tumornode-matastasis (TNM) staging of lung cancer.In patients with surgery, 13 groups and 14 groups of lymph nodes ignored because of the deep hidden in the lung.In this paper,the positive detection rate of 13 groups and the 14 groups of lymph nodes in non-small cell lung cancer (NSCLC) and their effects on pathological stage were studied.Methods 100 cases of NSCLC were collected from the Affiliated Hospital of Inner Mongolia Medical University as the research object,cut out the intrathoracic 2-12 group,13,14 lymph node metastasis rate for pathological examination,relationship factors of statistical analysis of the size of primary tumor,location,pathological type and lymph node.Results 100 cases of patients with intrathoracic lymph node metastasis rate was 47.0％,with the 10-12 groups lymph node,N2 lymph nodes,13,14 groups lymph node positive rate had significant differences (P＜0.05);T stage 13,14 groups of lymph node detection rate has statistically significant difference (P＜0.05);peripheral and central NSCLC stage N1 undetected rate no statistical difference (P＞0.05);various pathological types of tumors between N1 missed rate had no significant difference (P＞0.05).In addition,12 patients were found to have non-tumor-derived lobe lymph node metastasis.Conclusion It is necessary to detect the metastatic lymph nodes of 13,14 group and non-tumor-derived lobe in NSCLC.It is helpful to obtain accurate TNM staging and to guide postoperative treatment.