AIM: To observe the pathological role of 3-nitrotynosine (3-NT) on Escherichia coli LPS-induced vascular hyporeactivity in rats and the therapeutic effect of antioxidants. METHODS: Forty male SD rats weighting from 200 g to 250 g were randomly divided into four groups: the control group (n=10); LPS shock group (n=10); uric acid-treated group (n=10); melatonin-treated group (n=10). 6 h after LPS shock, phenylephrine (0.5-2.5 ?g?kg -1) was applied intravenously to all groups and the percentage increase in MAP was detected, respectively. The concentration-response curve of aorta rings from all groups rats were obtained by cumulative addition of phenylephrine (PE), and PE E_ max, EC_ 50 were calculated. The concentrations of plasma malondialdehyde (MDA), nitrate/nitrite and 3-NT were assayed in all groups 6 h after LPS shock. RESULTS: The MAP level induced by PE significantly decreased to 54.60% in LPS shock rats compared with the control (P

OBJECTIVE:To discuss the characteristics of drug-induced eye impairment.METHODS:206 cases of drug-induced eye impairment reported in internal medical journals published from Jan.1998 to Dec.2005 were analyzed statistically.RESULTS:The drug-induced eye impairment was mostly induced by anti-infective drugs,hormones,nervous system drugs.The impairment was manifested predominantly by glaucoma,keratitis,scleritis;retinopathy,hyphema,xeroma,damage of corneal epithelium and visual disorder etc.CONCLUSION:Clinicians should pay great attention to the drug-induced eye impairment for it can lead to a decline in eyesight or even loss of eyesight.

Objective To investigate the effective nursing measures of reducing the complications of intratra-cheal suction about mechanical ventilation patients to improve airway nursing quality. Methods 80 patients with ar-tificial air passage mechanical ventilation were divided into Control group (Group one, 40 cases) and Testing group (Group two, 40 cases) randomly. In the control group, suck at regular time after injecting normal saline solution in-to the in-airway (in order to diluted the sputum). There are improvement that nursing measures in testing group. For sample, suck sputums according to demands, choose appropriate sucking sputums tube, control the deepth of sucking sputum tube, adjust the negative pressure based on the deepth of sucking sputum tube, give pure oxygen two to three minutes before and after the suction, strengthen the airway humidfication, lubricate the sucking sputum tube with chlorotetracycline eye ointment, etc. Complications of intratracheal suction were compared in two groups. Results The comparison showed that the complications have a significant statistical difference in sputum obstruction, SpO2 de-scending to 80 percent and below during suction and airway mucous membrane traumatic hemorrhage. (P < 0.01). Conclusion The improved intratracheal suction will decrease the complications obviously like sputum obstruction, SpO2 reducing to 80 percent and below during suction and airway mucous membrane traumatic hemorrhage.

Objective:To summary the experience of the treatment of the patient in perioperative period with TAPVC.Methods:24 children(Male/Female 16/8)with TAPVC corrective operation were collected from March 2007 to June 2009,which age from 10 d to 182m(16.78?37.30)m and weight from 2.8～36 kg(7.00?6.83)kg.The care and treatment were applied to all cases after surgery,which included mechanical ventilation and continually low-does inotropic agents.Results:Two cases died postoperatively of pneumorrhagia and infection complicated with multiple organ dysfunction syndrome,respectively.The period of mechanical ventilation was 3～393(72.83? 80.63)h.The complication included low cardiac output syndrome(3 cases),pnmonia(11 cases),respiratory dysfunction(13 cases), arrhythmia(5 cases)and pneumorrhagia(1 case).Six cases were re-intubated children.Conclusion:TAPVC,especially with obstrction of pulmonary venous,should be surgically correced as earlier as possible to prevent pulmonary pertention and fibrosis of pulmonary venous.Pneumonia and respiratory dysfunction are the most important reason for prolonged ventilation time and ICU stay after cardiac surgery.Therefore,optimal respiratory management and prevention of low cardiac output will be beneficial for intensive care after TAPVC corrective operation.

Objective:To study the biological functions of bone marrow macrophage (BMM ?) and to establish the methodology of separation, purification, cultivation and identification of BMM ?.Methods:Using the techniques of anchorage-dependent culture of separated rat bone marrow cell (rBMC) in DMEM culture media (contain 20% horse serum,20% (v/v)L 929 conditioned media) in vitro,a lot of purified anchor cells were obtained, and these cells were identified with specifically biological marker of macrophage, such as 1, morphologicalobservation:invert phase contrast microscopy, light and electron microscopy;2, enzyme cytochemistry:acid phosphatase (ACP),?-acetic acid naphthol esterase (?-ANE);3,phagocytic experiment:phagocytosis of chicken erythrocytes and prepared Chinese ink;4,immunocytochemistry: surface specific antigen of macrophage ( CD 68 stain).Results:The cells were purified having functional satisfactory macrophage according to morphological observation,enzyme cytochemistry, phagocytic experiment and immunocytochemistry.Conclusion:This is a simple and easy method for separation, purification, cultivation and identification of rat marrow macrophage.

The concepts of Western medicine in treating tumor have changed markedly in recent years, and begun to put more emphasis on the whole survival status of patients. With recent emergence of targeted drugs, it was found that curative effects of the same targeted drugs in treating patients with the same tumor type even the same tumor pathological subtype but different therapeutic targets may be different. On the contrary, the curative effects of different targeted drugs in treating patients with different tumor types but the same therapeutic targets may be similar. Accordingly, the viewpoint of personalized therapy is advocated in Western medicine. Under the guidance of syndrome differentiation treatment in traditional Chinese medicine (TCM), patients with the same tumor and different syndromes should be treated by different herbs, and patients with different tumor and the same syndrome should be treated by the same herbs. Both the theories of "the same treatment for the same syndrome" and "the same treatment for the same therapeutic target" indicate that personalized therapy in Western medicine is similar to TCM syndrome differentiation treatment. The article focuses on "different treatments for the same disease" as well as "the same treatment for different diseases" to describe the similar points between personalized therapy and TCM syndrome differentiation treatment and thereby proves that TCM syndrome differentiation treatment is a scientific and essential approach in treating tumor. At the same time, the article also points out the problems and solution methods of TCM syndrome differentiation treatment.

Bacterial cellulose iunique and promising material aimplantand scaffoldin tissue engineering study.Iicomposed of pure cellulose nanofibemesh spun by bacteria.Aithigh watecontenand purity,iimore biocompatible fomultiple medical applications.In addition,iieasy to control itmechanical strength,chemical structure and morphology,therefore iimore suitable to be used abiomedical material in vivo.Bacterial cellulose habeen applied atissue engineering material in clinical medical aspects,foexample,wound dressingalternatives,artificial skin,artery eluting stent,bone tissue engineering scaffold,artificial blood vessel,artificial hearvalve prosthesis.The application of bacterial cellulose ialso extended to the construction of tissue engineering corneal stromand corneal contaclens.Thipapereviewthe progresin the applying spectrum of bacterial cellulose in corneal tissue engineering and othemedical field.

Objective:To investigate the effects and mechanism of Ginseng polysaccharides (GPS) on glycosaminoglycan synthesis in rat chondrocytes in vitro.Methods:Chondrocytes isolated from knee joint cartilage of rats were subcultured.After monolayers were established,chondrocytes induced by 20 ng·ml-1 IL-1β were treated with GPS respectively at the concentration of 2,10 and 50 ng ·ml-1for 48 h.The effects of GPS on chondrocytes were analyzed using MTT assay for chondrocyte proliferation and 1,9-dimethylmethylene blue assay for GAG content.mRNA content in xylosyltransferase-Ⅰ(XylT-Ⅰ),galactosyltransferase-Ⅰ(GalT-Ⅰ),galactosyltransferase Ⅱ(GalT-II) and galactose-β-1,3-glucuronosyltransferase Ⅰ(GlcAT-Ⅰ) and that in matrix-degrading enzymes matrix metalloproteinases-3 (MMP-3) and aggrecanase-1and aggrecanase-2 was also detected.Results:GPS had no toxic or proliferating effect on chondrocytes at all observed concentrations.GPS at the concentrations of 10 ng·ml-1 and 50 ng·ml-1 up-regulated GAG synthesis (P<0.01).10 ng·ml-1 GPS enhanced the mRNA expression of XylT-Ⅰ (P<0.01),and 50 ng·ml-1 GPS enhanced the mRNA expression of XylT-Ⅰ,GalT-Ⅰ and GalT-II (P<0.05,P<0.01).GPS did not inhibit the mRNA expression of matrix-degrading enzymes MMP-3 and aggrecanase-1and aggrecanase-2 enhanced by IL-1β.Conclusion:GPS can improve GAG synthesis in IL-1β stimulated chondrocytes in vitro,which is due to the expression promotion of GAG synthesis enzymes without the expression inhibition of matrix-degrading enzymes.

Colorectal cancer (CRC) generally occurs in older patients, however the proportion of young patients with CRC is increasing in recent years, which has drawn lots of scholar′s attention, but their research results were controversial. The current literature from several fields, including clinicopathological characteristic of young CRC patients, the optimal age cutoff between young and elder CRC patients, and the prognosis, early screening and diagnosis in young CRC patients will be reviewed.

Objective To explore the causes of misdiagnosis through clinical data analysis of primary Sjogren syndrome ( PSS) patients misdiagnosed as primary hypothyroidism disease.Methods The clinical data of 34 cases with primary Sjogren syndrome ( pSS ) who were misdiagnosed as primary hypothyroidism were retrospectively analyzed.The misdiagnosis reason was analyzed.Results In 34 patients,a total of 14 patients with primary hypothy-roidism could not explain the clinical signs and symptoms, included dry mouth, dry eyes, recurrent parotid gland enlargement,both hands reynolds phenomenon, purpura like rash and joint pain in the lower extremities.A total of 20 patients with primary hypothyroidism could not be explained by an auxiliary examination abnormality, including ESR,IgG,IgM or IgA increased,white blood cells and platelets,low blood potassium,pulmonary CT suggest interstitial lung disease,ANA (+) (>1:40),RF(+)(>1:20).Conclusion pSS misdiagnosis for hypothyroidism is not uncommon.Department of Endocrinology physicians should pay special attention to hypothyroidism can not explain the symptoms and auxiliary examination abnormal, make the necessary differential diagnosis to reduce the misdiagnosis rate.