ObjectiveTo investigate the clinical value ofDNA ploidy analysis in the diagnosis of benign and malignant pleural effusion.MethodsDNA ploidy in 24 benign pleural effusion and 39 malignant pleural effusion were detected by flow cytometry (FCM) and compared with the results of cytologic detection at the same time.ResultsThe positive rates of FCM detection in benign and malignant pleural effusion were 8.33％(2/24) and 64.10％ (25/39),there was significant difference (P＜0.05).The positive rates of cytologic detection in benign and malignant pleural effusion were 4.17％( 1/24 ) and 53.85％( 21/39),there was significant difference (P＜0.05).The sensitivity of FCM and cytologic detection in malignant pleural effusion was 64.10％ (25/39) and 53.85％ (21/39),the specificity of two methods was 91.67％ (22/24) and 95.83％ (23/24.),the results of two methods showed no significant differences (P ＞0.05).ConclusionDNA ploidy analysis by FCM has important clinical value in the diagnosis of benign and malignant pleural effusion.

Objective To study the related factors of coronary no-reflow phenomenon in H-hypertensive acute myocardial infarction (AMI) patients after percutaneous coronary intervention (PCI).Methods 120 AMI patients were selected as the study subjects,all patients were given PCI,the infarct-related coronary artery stenosis forward flow(TIMI) grade ≦ 2 level was the no-flow criteria.The patients were divided into two groups:the no-reflow group (35 patients),the other 85 cases classified as normal blood group.The age,gender,body mass index (BMI),history of diabetes,hyperlipidemia hyperlipidemia history,family history,blood glucose,homocysteine (Hcy),white blood cell count,C-reactive protein (CRP),erythrocyte sedimentation rate (ESR),creatine kinase (CK),creatine kinase (CK-MB),triglycerides,total cholesterol,high density lipoprotein (HDL),low density lipoprotein (LDL),troponin-Ⅰ (cTn-Ⅰ),treatment time and other factors were observed and compared.Logistic regression analysis was used to identify relevant factors of no-reflow.Results 120 patients with primary PCI,no-reflow in 35 patients,the incidence rate was 30.1％.The sex,smoking history,history of hyperlipidemia,diabetes,history of angina,and Killip classification before treatment,time from chest pain to before treatment between no-reflow patients and normal blood flow patients had no significant differences (t =1.275,P ＞ 0.05).Multivariate Logistic regression analysis showed that:age,diabetes,triglycerides,HCY,cTn-Ⅰ,CK-MB,CRP were related factors of no-reflow(t =3.427,P ＜ 0.05).Conclusion Age,without pre-infarction history of angina,coronary artery open long hours,admission NYHA low,white blood cell count high,CK-MB and cTn-Ⅰ levels higher are independent risk factors of no-reflow phenomenon,PCI with no-reflow phenomenon indicates the poor prognosis.

Objective: To evaluate the diagnostic value of IL-6,IL-8,and TNF-? in malignant pleural effusion and tuberculous pleural effusion.Methods:We detected IL-6,IL-8,and TNF-? in malignant pleural effusion(21 cases)and tuberculous pleural effusion(13cases).Results:The mean values of the three cytokines measured in tuberculous pleural effusion were significantly higher than malignant pleural effusion( P

Objective:To implove the level of diagnosis and treatment of renal tuberculosis complicated with active pulmonary tuberculosis.MethodsThe diagnosis and treatment of 28 cases with renal tuberculosis complicated with active pulmonary tuberculosis were analyzed.Results:88.2% of patients were negative in PPD test, and 55.6% positive in the sputum acid fast stain. All the patients except 4 received thetreatment of anti-tuberculosis continuosly for 3-6 months before surgery.Conclusion:It is necessary to treat the patients with anti-tuberculosis drugs and improve their immune function before proper surgical treatment.Patients in fair general condition could be operated on 2 weeks after anti-tuberculosis therapy.

Objective To study the application of the intelligent optimum tube voltage ( Care kV) combined with automatic tube current ( Care Dose 4D) technique in dual source CT scanning for the abdomen .Methods 180 adult patients underwent whole abdominal CT examination from February 2016 to March 2016 of were selected as the retrospective research objects .On the basis of reference mAs of the image quality the patients were divided into three groups using completely randomized grouping method with 60 patients in each group, such as 250 mAs group, 200 mAs group, and 150 mAs group.The other scanning parameters and image reconstruction parameters were the same .The liver parenchyma CT values and image noise( SD) at the second hepatic portal level were measured .The signal-to-noise ratios ( SNR) in the three groups were compared as well as contrast to noise ratio ( CNR ) , noise ( SD ) , the average volume CT dose index (CTDIvol), dose length product (DLP) and effective dose(E).Results The kV and mAs values in the plain scan , arterial phase scan and portal phase scan in the three groups of patients had statistically significant differences(F=35.25, P<0.05).With the reducing of the refference mAs, the frequency of choosing lower kV in three groups of patients increased gradually .The CTDIvol, DLP and E of plain scan, arterial and portal phase in three groups of patientshad significant difference (F=31.51, P<0.05).With the decline of the reference mAs, the radiation dose decreased gradually , with 150 mAs group lowest.The SNR, CNR and noise of three phases in 250 mAs group and 200 mAs group had no statistically significant difference (F=1.61, P>0.05).In 250 mAs group and 150 mAs group, the SNR and the noise of plain scan and arterial phase images had no statistically significant difference ( F=1.98, P>0.05) , and SNR of portal phase images and CNR of three phases had statistically significant difference (F=27.64, P<0.05);In group 200 mAs and group 150 mAs, the SNR, CNR and noise of plain scan and arterial phase images had no statistically significant difference (F=1.95, P>0.05), and the SNR and CNR of portal phase images had significant difference (F=19.63, P<0.05).Conclusions For the combined use of Care kV and Care dose 4D,it is feasible to select appropriate reference mAs in reducing radiation dose without sacrificing imaging quality .

Faced with huge demand for building public health capacity in the post-Ebola era, Africa Union has planned to establish Africa Center for Disease Control and Prevention ( Africa CDC,ACDC), with headquarters in Addis Ababa, Ethiopia, and at least 5 regional collaborating centers in each region of the continent .The strategic objectives of ACDC focus on building a continental integrated public health system and strengthening needed public health capacity .The current study analyzed the background , plans, development layout and strategy of ACDC , and provided references for China-Africa cooperation in the areas of public health and for the formulation of China global strategies of biosecurity and disease prevention and control .

Objective To study the surgical treatment of duodenal invasion by cancer of hepatic flexure of (colon). Methods Clinical data of 15 cases of duodenal invasion by cancer of hepatic flexure of colon were analyzed retrospectively.Results Invasion of the second or third portion of the duodenum by cancer of the hepatic flexure of colon occurred in 15 cases.The duodenum was adhered to the cancer in 6 cases,(seromuscular) invasion by the tumor in 5 cases,and duodenocolic fistula in 4 cases. All of the cases received surgical (treatment). 8 cases were mucoid adenocarcinomas, 4 were poorly differentiated adenocarcinomas, two were moderately differentiated adenocarcinomas, and one was signet-ring cell carcinoma. One patient died (after) operation, and the five-year survival rate was 46.7%(7/15). Conclusions Duodenum is the first organ easily to be invaded by cancer of hepatic flexare of colon. Radical and en bloc resection of the colonic cancer and involved organs is the best therapeutic method. The key to recovery is correct treatment of the (surgical) defect of the duodenum.

ObjectiveTo study the effect of fibrin glue(FG) and growth hormone(GH) on the anastomosis of the small bowel at peritonitis. MethodsResection and anastomoses of the small bowel at peritonitis was performed in rats. In group N:normal control;in group A:a standard anastomosis was constructed with 12 silk sutures;in group B:an incomplete anastomosis was constructed with only 4 silk sutures;in group C: incomplete anastomosis+FG;in group D: incomplete anastomosis+GH;in group E: incomplete anastomosis+FG+GH.On days 1,3 and 5,ten rats in each group were sacrificed.The healing of anastomosis were evaluated,the anastomotic bursting pressure and hydroxyproline concentration were measured. Results In group A the bursting pressure was low from day 1 to day 3(18?10?mm?Hg and 36?11?mm?Hg each).The percent of false-healing was 30%,significantly higher than group N.The bursting pressure in group C was significantly higher than in group A(32?7?mm?Hg and 65?9?mm?Hg each),the healing of the anastomosis was significantly improved,the percent of false-healing was 11.7%.Hydroxyproline concentration of each group showed no differences. Conclusion Fibrin glue helps the healing of the anastomosis of the small bowel at peritonitis in rats,and can protect it from complications. The addition of GH does not improve healing.

OBJECTIVE:To establish an HPLC method for the determination of diphenyl ethylene glycoside in Yishi oral solution. METHODS:The sample was separated on a DiamonsilTM C18 column(200 mm?4.6 mm,5 ?m) with the mobile phase consisted of acetonitrile-0.1% phosphoric acid(18∶82) at a flow rate of 1.0 mL?min-1.The detection wavelength was 320 nm and the column temperature was 40 ℃.RESULTS:The linear concentration range of diphenyl ethylene glycoside was 65.25～625.50 ng(r=0.999 9) and the average recovery rate was 100.07%(RSD=0.25%,n=6). CONCLUSION:The method was proved to be simple,re-producible and simple in operation,and it can be used for the quality contro1 of Yishi oral solution.