[Objective] Discussion on academic ideas and clinical experience of Professor Fu Ping in treatment of polycystic ovary syndrome. [Methods] From the etiology and pathogenesis, therapeutic principle and therapy to expound the academic viewpoints and clinical experience of Professor Fu Ping in treatment of polycystic ovary syndrome, the prescription and Chinese medicine conditioning characteristics are summarized, and exemplify that. [Results] Professor Fu Ping believes that the pathogenesis of this disease is a virtual reality, such as phlegm and blood stasis;according to the etiology and pathogene-sis, strengthening the body resistance to eliminate pathogenic factors, coordinating Chong and Conception Vessels is the main rule; dissipating phlegm and removing dampness, warming the kidney and strengthening the spleen, nourishing liver kidney, regulating qi and activating blood is the clinical common therapy, clinical y obtaining better therapeutic effect. [Conclusion] The clinical experience of Professor Fu Ping in treatment of polycystic ovary syndrome is effective, has the value of popularization and application.

OBJECTIVETo observe the effect of Pi transportation, dampness resolving and phlegm expelling herbs (PTDRPEH) on the obesity degree, fat hormones, and leptin resistance in diet-induced obesity (DIO) rats.

METHODSAmong the 120 Wister rats, 10 were recruited as the blank control group (fed with basal forage), and the remaining 110 were administered with high-fat high-nutrition forage for 17 weeks. According to weight, we obtained 40 DIO rats and 10 diet-induced obesity resistance (DIO-R) rats. DIO rats were further divided into four groups, i.e., the DIO model group (normal saline, at the daily dose of 2 mL), the sibutramine group (at the daily dose of 1.6 mg/kg), the dampness resolving and phlegm expelling group (DRPE, at the daily dose of 3.2 g/kg), and the Pi transportation group (PT, at the daily dose of 3.2 g/kg). All were given by gastrogavage. Normal saline (2 mL) was given by gastrogavage to rats in the blank control group and the DIO-R group. The basal forage was administered to rats in the blank control group, while high fat forage was continually given to rats in the remaining five groups. Their body weights and body lengths were measured after 16 weeks of gastrogavage. All intra-abdominal fat was taken out to measure the degree of obesity and fat contents. Insulin resistance index (IRI), blood glucose, triglycerides, cholesterol, leptin, neuropeptide Y (NPY), tumor necrosis factor alpha (TNF-alpha), and adiponectin were detected after blood withdrawing. Leptin, TNF-alpha, adiponectin, suppressors of cytokine signaling-3 (SOCS-3), and other relevant adipose hormones and inflammatory cytokines were examined in the fat homogenate.

RESULTSCompared with the blank control group, DIO model rats' body weight, body mass index (BMI), fat factor, IRI, serum leptin, TNF-alpha, and SOCS-3 significantly increased (P < 0.05, P < 0.01); serum NPY, serum leptin, and adiponectin decreased (P < 0.05). Leptin increased and NPY decreased in DIO-R model rats. Compared with the DIO group, DIO-R model rats' body weight, BMI, fat factor, IRI, serum NPY, TNF-alpha, and SOCS-3 all decreased (P < 0.05, P < 0.01); leptin and adiponectin in serum and the fat homogenate all increased (P < 0.05, P < 0.01). After intervention with Sibutramine, rats' body weight, BMI, fat factor, and TNF-alpha in the fat homogenate obviously decreased (P < 0.05, P < 0.01). Serum TNF-alpha decreased, leptin and adiponectin increased in rats of the DRPE group (P < 0.05, P < 0.01). BMI, fat factor, IRI, leptin, and SOCS-3 showed a decreasing tendency, but with no statistical difference (P > 0.05). The body weight, BMI, fat factor, IRI, TNF-alpha, and SOCS-3 all decreased in the PT group (P < 0.05, P < 0.01); leptin and adiponectin in the serum and the fat homogenate increased (P < 0.05, P < 0.01).

CONCLUSIONSSibutramine could reduce body weight and TNF-alpha in the adipose tissue. Herbs of PT could inhibit fat diet-induced obesity and insulin resistance (IR), with superior effect to herbs of DRPE. Its mechanism might be closely related to promoting leptin and adiponectin secreted by fat, reducing leptin resistance, and elevating serum levels of leptin and adiponectin.

Adiponectin ; blood ; Animals ; Diet, High-Fat ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; Insulin Resistance ; Leptin ; blood ; Male ; Obesity ; blood ; drug therapy ; Rats ; Rats, Wistar ; Tumor Necrosis Factor-alpha ; metabolism

Acquired Immunodeficiency Syndrome ; prevention & control ; Humans

Objective:To explore the necessity of monitoring polysomnography (PSG ) in snoring children.Method:The PSG were monitored in 162 children with snoring or apnea.Acording to results of PSG,162 cases were separated into two groups:group obstructive sleep apnea hypopnea syndrome(OSAHS) and group primary snoring(PS),monitoring data including:apnea hypopnea index(AHI),snoring index(SI),lowest oxygen saturation(LSaO_2) and time of SaO_2<0.9,all data were undertaked statistical analysis. Result:In all 162 snoring cases,150 cases(92.6%) belong to group OSAHS,12 cases(7.4%) belong to group PS;Differences of data of age,sex and body mass index(BMI) are not significant but that of AHI,SI,LSaO_2 and time of SaO_2<0.9 are significant(P<0.05).Conclusion:It's necessary to monitor PSG in snoring children for early diagnosis and correct treatment to avoid disadvantage of OSAHS.

Acquired Immunodeficiency Syndrome ; prevention & control ; therapy ; Communicable Disease Control ; Humans

From information and consent,the article explores the history,connotation,element and the problems encountered in practice of informed consent.The paper aims to provide help for resolving realitical problems by these theoretical discussion.

Anti-Arrhythmia Agents ; adverse effects ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; Humans

Acquired Immunodeficiency Syndrome ; prevention & control ; China ; Humans

Objective To explore the clinical effect by using of 654-2 to multigravidae.Methods Among 60 multigravidaes deliverying,30 were treated with 654-2 and oxytocin,the other 30 multigravidaes were treated with oxytocin only for control.The time of dilatation of the cervix,the rate of fetal distress and neonatal asphyxia,the rate of operative delivery and postpartum hemorrhage were observed.Results The time of dilatation of the cervix and the rate of operative delivery were shorter than the control.It was significantly different between the two groups(P0.05).Conclusion 654-2 can promote the first stage of the multigravidae.It can reduce the rate of operative delivery.It is safety by using of 654-2 to short the first stage to multigravidae.

Aim To investigate the effect and mechanism of action of chronic morphine treatment on the neurosteroidogensis of primary cultured rat cerebral cortical neurons(CCNs).Methods The effect of morphine on the production of pregnenolone(PREG),dehydroepiandrosterone(DHEA),allopregnanolone(AP),pregnenlolone sulfate(PS)and dehydroepiandrosterone sulfate(DS)in cell culture media was measured by solid-phase extraction and LC-MS,with methyltestosterone(MT)or estrogen sulfate(ES)as internal standards.The dependence-like changes of CCNs were determined by testing the p-CREB level in the nuclear lysates using western blot.Results Compared with the control group,morphine treatment significantly reduced levels of PREG and DS respectively;opioid agonist DAMGO significantly reduced levels of PREG,DS and PS respectively,and increased the level of AP significantly.Compared with the morphine group,?-opioid-antagonist CTAP concomitant with morphine increased the level of PREG significantly.Compared with the control group,chronic morphine treatment or DAMGO treatment significantly increased the level of p-CREB in the CCNs.Compared with the morphine treatment group,?-antagonsit CTAP significantly reduced the level of p-CREB.Conclusion ?-opioid receptor mediated the inhibitory effect of morphine on levels of neurosteroids,and changes of neurosteroid levels might be related to morphine dependence.