Objective To explore the effect of different speed of bladder irrigation after transurethral resection of prostate.Methods 477 cases of benign prostatic hyperplasia,who received transurethral resection surgery were collected.According to the date of surgery,they were divided into observation group(in a single day,249 cases)and control group(double day,228 cases).The normal saline was used in the two groups,and the control group was washed with the conventional bladder irrigation,and the observation group was treated with the early rapid(no speed limit opening)and the routine velocity alternating flushing.The incidence of bladder spasm,the incidence of bladder spasm,the bleeding of bladder and the changes of heart rate and the mean arterial pressure of 24h were compared. Results Urine turned clear time,the total fluid,postoperative 5d red blood cell count of the observation group[(19.8 ± 8.3)h,(44.7 ±6.1)L,(25.8 ±11.3)months/μL]were significantly lower than the control group[(25.4 ±9.0)h, (52.3 ±7.4)L,(33.4 ±10.1)months/μL],the differences between the two groups were statistically significant(t =-6.270,-2.417,-10.459,all P <0.05).The incidence rate of bladder spasm,seizure frequency and duration per capita of the observation group[24.5%,(2.2 ±0.6)times,(15.4 ±4.6)min]were significantly lower than the control group[46.9%,(6.7 ±1.2)times,(27.6 ±5.1)min],the differences between the two groups were statistically significant(χ2 =8.725,t =-13.250,t =-18.066,all P <0.05).The postoperative rinse 1h,2h and 24h heart rate,mean arterial blood pressure had no statistically significant differences(all P >0.05).Conclusion The early rapid and conventional alternating washing method can improve the seizure of bladder spasm after prostatectomy,and reduce the bleeding of bladder.

Objective To investigate the pathogenesis of interleukin-21 (IL-21)role in primary biliary cirrhosis (PBC).Meth-ods 38 cases of PBC were selected for the study.35 caeses of healthy subjects were selected as the control group in January 2012 to January 2014 from Shenzhen Fuyong People’s Hospital of Baoan District and the Third People’s Hospital of Shenz-hen.The levels of IL-21,IL-17 of two groups were measured with Enzyme-linked immunosorbent assay (ELISA).The levels of T helper lymphocytes 17 (Th17),regulatory T cells (Treg)cells ratios of two groups were measured with flow cytome-try.The relationship of IL-2 1 and liver function were analyzed with pearson correlation.Another analysis of pyruvate through the establishment of in vitro deaminase complex E2 subunit (PDC-E2)peptides relationship with Th17 and Treg cell.Results The levles of IL-17 (18.23±4.12)ng/L,IL-21 (512.25±18.25)ng/L,Th17 (3.98±0.98)%,Th17/Treg (39.25±3.25)% of PBC group were significantly higher than the control group (7.89±3.26)ng/L,(212.98±21.52)ng/L,(0.92±0.21)% and (12.98±3.12)%,while the PBC group Treg (3.11±0.85)% were lower than the control group (8.36±1.12)%.IL-21 were positive correlation with IL-17,Th17 (t=8.886,7.021,4.448,10.112,5.125,P<0.05).In vitro experiments showed that the addition of IL-21 in the culture system,the levels of Th17 (2.41±0.82)%,Th17/Treg cells(32.98±3.12)% were significantly higher than the control group(0.98±0.21)%,(12.25±3.25)%,while Treg (4.75 ±0.68)% were lower than the control group(11.85±0.96)%,the difference was statistically significant (8.521,6.489, 8.236,P<0.05).Conclusion IL-21 levels increased with the pathogenesis of PBC has a close relationship,which can pro-mote the reaction of PDC-E2 Th1 7/Treg immune imbalance caused thereby occurs mediated by PBC.

Colorectal carcinogenesis is the result of accumulation of genetic and epigenetic alters.Gene mutations and changes in DNA methylation patterns,resulted from genomic instability,are the main molecular events in colorectal cancer.Identification of key mutations and methylation phenotypes in genes leading to colorectal cancer progression,and molecular pathology classification and diagnosis are likely to be the prerequisites for individualized and targeted treatment.

Objective To determine the prevalence and influencing factors of urinary calculi,to explore the interIrelationships between urinary stone disease and various risk and protective factors,to determine the potential implications for intervention and prevention in Changsha,and to evaluate the relative importance of each risk factor,with the objective of providing scientific guidelines for urinary calculi prevention and diagnosis. Methods A case-control study was designed,and 66,772 people were surveyed.Ultrasound examination,blood biochemistry,general physical examination,gender and age were asked in a questionnaire to determine a diagnosis of urolithiasis.By means of SPSS software a x2 tendency test and non-condition Logistic regression were conducted. Results A multivariate Logistic regression analyses was conducted.The final factors entered into the model were sex,age,profession,dietary habits,drinking habits,smoking and level of uric acid. Conclusions Hazard factors associated with urolithiasis are male gender,age,profession,dietary and drinking habits,smoking and level of uric acid.

Circulating tumor cells (CTCs) in breast cancer patients can be detected by Cell Search system. Results from recent studies suggested that CTCs level might serve as a prognostic marker and be used in the early assessment of therapeutic response in patients with metastatic breast cancer. However the role of CTCs in early stage breast cancer is not well established. Large prospective trials are needed to further understand its biological characteristics and to confirm its role as a predictive and prognostic marker.

Objective:To research the quality standard for Xilei San. Methods:Imdigo naturalis and calculus bovis were identi-fied by TLC and their contents were determined by HPLC, and Borneolum syntheticum was determined by GC. Results: Indirubin showed good linearity over the concentration range of 0. 043-4. 255 0 μg(r=0. 999 2) with the average recovery of 97. 9%(RSD=1.5%,n=6). Bilirubin showed good linearity over the concentration range of 9.968 ×10 -4-9.968 ×10 -2 μg(r=0.999 9) with the average recovery of 95.3%(RSD =0.55%, n =6). Borneolum syntheticum showed good linearity over the concentration range of 0. 010 3-0. 826 6 mg(r=0. 999 8) with the average recovery of 94. 9%(RSD=3. 2%,n=6). Conclusion: The method is accurate with good reproducibility,which can be used as the quality control for Xilei San.

Objective To describe clinical and functional features of patients with left main coronary artery (LM) stenosis. Methods Significant stenosis was defined as ≥ 50%.One hundred and eighty-eight patients with LM stenosis and 200 patients with clinically suspected coronary heart disease (CHD) without LM stenosis were enrolled. Results (1) The incidence rate of LM stenosis was 5.59%.(2) Patients with LM stenosis all had risk factors.Furthermore,featured older age,higher incidence of angina pectoris,and the same incidence of myocardial infarction history when compared with the patients without LM stenosis.(3) The left ventricular ejection fraction was lower in patients with LM stenosis than that in patients without LM stenosis,and it was lower too in patients with isolated LM stenosis than in patients with LM stenosis accompanied by triple vessel stenosis.The left ventricular end diastolic pressure showed no significant difference among various groups. Conclusion Patients with LM stenosis feature older age,severe angina pectoris.Furthermore, most of them are accompanied by other vessel lesions.Most LM stenosis are located at the ostium and the crotch of LM is presented as stenosis

Objective To observe the clinical effect of psychological intervention combined with somatostatin in the operation of pancreas. Methods From January 2016 to August 2017, 68 cases of pancreatic surgery were treated in our hospital. The patients were randomly divided into the observation group (n=34) and the control group (n=34). All patients were treated with somatostatin after the operation of pancreas. The patients in the observation group were treated with positive psychological intervention, and the effect of the application was observed. Results After the intervention, the two groups of patients with SAS and SDS were improved, the improvement is more obvious in the observation group (P<0.05);the observation group anal exhaust time, patients with gastrointestinal peristalsis time, bed time, hospitalization time were significantly shorter than the control group (P<0.05). Conclusion The combination of psychological intervention and somatostatin can improve the psychological status and promote postoperative recovery of patients after pancreatic surgery.

OBJECTIVE:To analyze volatile oil from the roots and fruits of Kadsura longipedunculata. METHODS:GC-MS method was adopted. The determination was performed on HP-5MS quartz capillary column with nitrogen as carrier gas at the flow rate of 0.9 mL/min. The temperature of sample inlet was 250 ℃,the initial temperature of column was set at 50 ℃(temperature programming). The column pressure was 80 kPa;the ratio of split sampling was 40 : 1. The sample size was 1.0 μL. Mass chroma-tography was as follows:electron bombardment ion source as ion source;electron energy of 70 eV;inlet temperature was 230 ℃;scanning range of m/z 40-400,scanning interval time of 1.0 s. RESULTS:Totally 43 chromatographic peaks were detected in vola-tile oil from the roots of K. longipedunculata,and 24 peaks were identified,accounting for 91.5%;main components were sesqui-terpenes and monoterpenoids,among which the relative contents of (+)-delta-cadinene,γ-cadinene and isovalencene were in high level. Totally 52 chromatographic peaks were detected in volatile oil from the fruits of K. longipedunculata,and 36 peaks were iden-tified,accounting for 82.2%;main components were sesquiterpenes and monoterpenoids,among which relative contents of caroph-yllene,γ-cadinene and valencene were in high level. CONCLUSIONS:The study basically confirm main volatile components in volatile oil from the roots and fruits of K. longipedunculata,and the roots and fruits of K. longipedunculata have significant differ-ence.

Objective:To explore the effect of sodium cantharidate vitamin B6 injections in the adjuvant treatment of advanced cer-vical cancers. Methods:Totally 60 cases of advanced cervical cancer patients were randomly divided into the observation group and the control group, and the control group was given common DP chemotherapy and radiotherapy, while the observation group was given sodium cantharidate vitamin B6 injections additionally. After the 6-week treatment, the short-term curative effect, bone marrow inhibi-tion adverse reaction, immune function and life quality were compared between the two groups. Results:The short-term curative effect in the observation group was 66. 7%, which was significantly higher than that in the control group (40%)(P<0. 05). The degree of adverse reaction was significantly lower than that in the control group with statistical significance (P<0. 05). After the treatment, CD8 +, NK cells and CD8 + / CD4 + in the two groups were significantly improved, and those in the observation group were all better than those in the control group (P<0. 05). The improvement of life quality in the observation group was more notable than that in the control group with statistical significance (P <0.05). Conclusion: Sodium cantharidate vitamin B6 injections can significantly in-crease the short-term curative effect and immune function, decrease bone marrow inhibition and improve life quality of advanced cervi-cal cancer patients.