Objective:To research the quality standard for Xilei San. Methods:Imdigo naturalis and calculus bovis were identi-fied by TLC and their contents were determined by HPLC, and Borneolum syntheticum was determined by GC. Results: Indirubin showed good linearity over the concentration range of 0. 043-4. 255 0 μg(r=0. 999 2) with the average recovery of 97. 9%(RSD=1.5%,n=6). Bilirubin showed good linearity over the concentration range of 9.968 ×10 -4-9.968 ×10 -2 μg(r=0.999 9) with the average recovery of 95.3%(RSD =0.55%, n =6). Borneolum syntheticum showed good linearity over the concentration range of 0. 010 3-0. 826 6 mg(r=0. 999 8) with the average recovery of 94. 9%(RSD=3. 2%,n=6). Conclusion: The method is accurate with good reproducibility,which can be used as the quality control for Xilei San.

Objective To explore the levels of T lymphocyte subsets in asthmatic children and discuss their relationship.Methods 30 children with acute asthma attacks were divided ito disease control(bronchopneumonias)group(25 cases)and healthy control group(14 cases).Flow cytomerty was used to analyze the T lymphocyte subsets in them.Results CD 4+ and CD 4+/CD 8+T cell of the patients of the maderate to serere degree were both significantly higher than those in normal control.Conclusion(1)CD 4+ T cell plays an important role in asthma chronic airway inflammation.(2)There were no significant difference in the level of CD4+ T cell,CD8+ T cell,CD4+/CD8+ T cell between the patients with asthma of mild degree and those of moderate or severe degree group,but CD 8+ T cell a little higer in the latler patlents,which still needs further research.

Objective Retrospectively discuss the effect of deep anesthesia on the post-operative complications with CPB. Methods All of the chosen patients including anesthesia group (30 cases) and deep anesthesia (30 cases) had the heart surgery under general anesthesia and CPB with the age of (56?6.4), the time of CPB (148?23.6) minutes, Medtronic membrane oxygenator, 4:1 cold blood cardioplegia, the flo of 2.0～2.4L?min -1 ?m -2 .The relative markers: operatively, the mean artery pressure (MAP), end tip oxygen saturation (SpO_2),the mean quantity of regitine (Regitine);postoperatively, the mean base-excess (BEpost), the mean quantity of 5% sodium bicarbonate per kilogram (NaHCO_3post),oxygen index, the level of blood glucose ,the duration of mechanical ventilation and intensive care unit stay , wound healing up, and the mortality. Results With deep anesthesia, the patients had more stable hemodynamic reserve operatively and significant improvements in the balance of acid-base, the lung function, the blood sugar , the wound recovery and the mortality. Conclusion The complication of postoperation with CPB is affected by the degree of anesthetic depth, and the proper depth of anesthesia can reduce the post-operative morbidity and mortality.

Objective The expression of IL-13 in plasma and renal tissue was studied to eluciate the molecular pathogenesis of Mesangial proliferative glomerulonephritis (MsPGN). Methods Plasma level of IL-13 in patients with MsPGN and in normal control groups were examined by ELISA. The expression of IL-13 in patients with MsPGN and in control group were examined by immune histochemistry ABC technique in renal tissue.Results Plasma levels of IL-13 in patients with MsPGN were significantly higher than that in control group,and the highest was found in IgAN;IL-13 expressions in glomerulus and tubulointerstitial zone in patients with MsPGN were higher than that in control group, and IL-13 expression in tubulointerstitial zone was more strong,but it was not distinctive difference between non-IgA MsPGN and IgAN. Conclusion IL-13 may play an important role in the molecular pathogenesis of MsPGN.

Objective To investigate the application opportunity and effect of B-Lynch suture in severe postpartum hemorrhage during cesarean section. Methods One hundred patients with high risk to postpartum hemorrhage were randomly divided into two groups. Group A was given with preventive treatment by B-Lynch suture after the placenta was born. Group B was given with traditional treatment, such as kneading the uterus, pressing, "8" suture, stuffing with gauze first. B-Lynch suture was used after the failure of traditional treatment or the amount of bleeding was over 400 ml.At the same time, another 50 patients without severe postpartum hemorrhage were selected as control (group C). Observed the difference of the amount of bleeding during different time, the operation time and comphcations among the three groups. Results The amount of bleeding during operation and 2 h after childbirth in group A [(266.00±29.90),(301.33±40.30) ml] were obviously less than those in group B[(512.67±202.12), (554.17±201.78)ml](P<0.01). The operation time in group A [(33.73±2.28)min] was shorter than that in group B [(49.33±10.40) min] (P<0.01). The incidence rate of severe postpartum hemorrhage in group A (6%, 3/50) was lower than that in group B (64%, 32/50) (P<0.01). Every parameter in group A was similar with group C. There were no serious complications during and after operation. Conclusions The preventive use of B-Lynch suture in patients with high risk to postpartum hemorrhage attains much better effects, and depresses the incidence rate of severe postpartum hemorrhage, and also improves the living quality. B-Lynch suture should be. used widely in patients with high risk to postpartum hemorrhage preventively.

ObjectiveTo observe the clinical efficacy of acupuncture-moxibustion in treating tinnitus.MethodNinety-eight patients with tinnitus were divided into a warm-needling group (n=32), an acupuncture group (n=34), and a medication group (n=32), to observe thetherapeuticefficacies.ResultThe total effective rate was 83.3% in the warm-needling group, versus 78.8% in the acupuncture group and 60.0% in the medication group.ConclusionWarm needling and acupuncture plus TDP are both superior to the medication treatment in comparing the therapeutic efficacy.

Objective To analyze the relationship among HIF-1α, ERCC1 gene polymorphisms and the effects of platinum-based chemotherapy for the treatment of advanced non-small cell lung cancer (NSCLC) Methods HIF-1α C1772T and ERCC1 C118T loci were genotyped with PCR-RFLP method. The relationship between genotypes and the outcome of treatment as well as TTP was investigated. Results HIF-1α and ERCC1 of 102 patients were under the Hardy-Weinberg equilibrium. Patients with HIF-1α CT+TT genotype showed a significantly higher chemotherapeutic efficiency compared with patients with CC genotype; Patients with ERCC1 CC genotype showed a significantly higher chemotherapeutic efficiency compared with patients with CT+TT genotype. The median TTP was 7.0 months for the 98 patients followed-up; 7.0 months for HIF-1α CT+TT genotype, and 6.0 months for CC genotype, and the difference showed no significance; The median TTP was 7.0 months for ERCC1 CC genotype, and 6.0 months for CT+TT genotype, and the difference was statistically significant. Conclusions Patients with HIF-1α CT+TT genotype or ERCC1 CC genotype have showed higher chemotherapeutic efficiency, and patients with ERCC1 CC genotype show longer median TTP.

Objective To investigate the gene expression change of TNF-α,IL-6 and IL-1β at different time points in brain tissues of rats with cerebral ischemia reperfusion injury.Methods A total of 24 adult male SD rats were randomly divided into four groups:sham group and 3 groups with brain ischemia reperfusion of 3h,6h and 12h.Real-Time PCR was used to analyze the gene expression ofTNF-α,IL-6 and IL-1 β at 3h,6h,and 12h after reperfusion.Results In the sham group,the mRNA expression levels of TNF-α,IL-6 and IL-1 β in were low,but increased immediately after brain ischemia injury and decreased gradually thereafter.The gene expression of TNF-α mRNA at 3h after reperfusion was significantly increased and reached the peak (P ＜0.01) then significantly decreased at 12h after reperfusion.The gene expression of IL-6 mRNA was notably increased at 3h after reperfusion and peaked at 6h (P＜0.01),and significantly decreased at 12h compared with 6h (P＜0.01).The gene expression of IL-1 β mRNA at 3h after reperfusion was significantly increased,peaked at 6h (P＜0.01) and significantly decreased at 12h (P ＜0.01).Conclusion The gene expression levels of TNF-α,IL-6 and IL-1β mRNA increased significantly in the early stage of reperfusion and decreased gradually after reaching the peak,which suggested that the gene expression change of TNF-α,IL-6 and IL-1β was involved in the mechanism of cerebral ischemia reperfusion injury.

Objective: To study the effect of processing on the content of organic acids in the different processed products of Fructus Crataegi. Methods: The determinations of the crude Fructus Crataegi, five kinds of different processed products and newly fried product were carried out by titration. Results: There were significant differences in organic acid contents among different processed products and the crude. Conclusion: Different processed method and temperature can effect the contents of organic acid on Fructus crataegi.

Objective: To intvestigate the effects of processing on contents of matrine and oxymatrine of Radix Sophorae Flavescentis. Methods: The determinations of matrine and oxymatrine were carried out by dual wavelength TLC scanning. Results: There were obvious differences between the crude and the different processd products of Radix Sophorae Flavescentis in matrine and oxymatrineConclusion: The processing temperature, material, wine and vinegar can make the contents of matrine and oxymatrine of Radix Sophorae Flarescentis change.