Aim To purify monoclonal antibody in mouse ascites by a modified membrane affinity chromatography(MAC). Methods Human serum albumin HSA was adsorbed on the zeta-bind membrane (ZBM), a positively charged nylon membrane filter. Then the mouse aecites containing mAbs were filtrated through the ZBM to cause the mAbs bind to HSA adsorbed on ZBM. Finally,the purified mAbs were dissociated from the ZBM with guanidine hydrochloride solution. Results A sheet of ZBM(diameter 50mm) absorbed with HSA filtrated by 10 mL ascites could reach its maximum mAb binding capacity after two rounds of re-fitration. The dissociation of mAb from ZBM need only one round of filtration of dissociating solution. The purified mAb displayed a single band after PAGE. Sensitivity of detedcting HSA with purified mAbs was 20-fold higher than that with unpurified ascites in dot innunogold filtration assay(DIGFA). Conclusion The modified MAC with ZBM is a much easier, time-saving and effective method for affinity purification of antibodies in ascites.

Integron was a novel bacterial resistance gene horizontal transmission element.In recent years, many researchers made a lot of research on the resistance mechanism of pathogen.In this paper, the detection and the novel discovery of the integron gene cassette were summarized , including Enterobacteria, Acinetobacter baumanii, Pseudomonas aeruginosa and other bacteria , on the basis of the multidrug resistance mechanism mediated by integron.The prospect the research was descixbed and more attention should be paid to the prevention and control of multidrug resistant bacteria.

Objective To investigate effect of irinotecan combined with cisplatin on level of Bcl-2 and MACC1 protein in serum in patients with cervical cancer.Methods 92 cases of patients with cervical cancer surgery from department of gynaecology and obstetrics,the third People’s hospital of Nanchang were randomly divided into control group and treatment group.Control group was treated with paclitaxel therapy chemotherapy, treatment group were treated with irinotecan combined with cisplatin.Bcl-2 and MACC1 protein in serum were compared before and after the treatment. Results The level of Bcl-2 and MACC1 in both groups were not statistically significant pre-treatment;After treatment, the levels of Bcl-2 and MACC1protein in serum were decreased (P<0.05).Compared with control group post-treatment, the level of Bcl-2 and MACC1 protein in serum were lower in treatment group (P<0.05).During follow-up, death cases of treatment group and control group were 5 cases and 6 cases respectively, the survival rate of 89.13% and 86.96% respectively within the past six months(χ2 =0.103,P>0.05).Death cases of treatment group and control group were 4 cases and 12 cases respectively. the survival rate of 91.30% and 73.91% respectively within one year (χ2 =4.84, P <0.05 ) Conclusion Irinotecan combined with cisplatin in the treatment of cervical cancer in one year survival rate is higher, which may be related to the decrease of serum levels of Bcl-2 and MACC1 protein content.

Objective: To compare the ocular parameters of acute angle closure glaucoma (AACG) and chronic angle closure glaucoma (CACG). Methods: Totally 106 patients with primary angle closure glaucoma were recruited: 58 patients with AACG and 48 with CACG. All patients were divided into 3 groups: AACG attack eyes group, AACG uninvolved fellow eyes group and CACG group and underwent the same ophthalmic examinations, comprising optometry, keratometry, and A-scan ultrasonography. The lens/axial length factor (LAF) and relative lens position (RLP) were calculated. Results: The AACG attack eyes had a significant shallow anterior chamber depth, thick lens, short axial length and larger LAF. hTere tended to be a reduction in the percentage of LAF>0.20 in AACG attack eyes, CACG eyes and AACG uninvolved fellow eyes, though there were no statistically signiifcant difference in all groups (P>0.05). Conclusion: The eyes with AACG attack have a more crowded anterior chamber structure compared with uninvolved fellow eyes and eyes with CACG.

Objective To observe the clinical efficacy of Qishen Yiqi Dropping Pills combined with telmisartan on early diabetic nephropathy with Qi deficiency and blood stasis syndrome and changes of oxidative stress indicators. Methods Sixty patients with early diabetic nephropathy of qi-yin deficiency and blood stasis syndrome were randomly divided into treatment group (30 cases) and control group (30 cases). Both groups were given diabetes education, diabetes diet control and insulin injection. Patients in the control group took telmisartan orally, and patients in the treatment group were given Qishen Yiqi Dropping Pills additionally. The treatment course was 12 weeks. The indicators were observed before and after treatment including blood pressure (BP), fasting blood glucose (FBG), glycated hemoglobin (HbA1c), urinary albumin excretion rate (UAER), urinary 8-iso-PGF2α excretion rate, serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malonaldehyde (MDA), and the clinical efficacy was evaluated. Results The total effective rate of the treatment group was significantly higher than that of the control group, with statistical significance (Z=2.822, P=0.005). After treatment, the indicators including BP, FBG, HbA1c, serum MDA, UAER and urinary 8-iso-PGF2α excretion rate all decreased significantly in both groups (P<0.05). Serum SOD and GSH-Px increased significantly at the same time (P<0.05). BP, FBG and HbA1c were not statistically different between the two groups (P>0.05). However, serum MDA, UAER, and urinary 8-iso-PGF2αexcretion rate were significantly lower (P<0.05), and serum SOD and GSH-Px were significantly higher in treatment group than control group (P<0.05). Conclusion Qishen Yiqi Dropping Pill combined with telmisartan is effective in reducing proteinuria in patients with early diabetic nephropathy of qi-yin deficiency and blood stasis syndrome. Its protective mechanism for kidney is possibly related to inhibiting oxidative stress injury at the early stage of diabetic nephropathy.

Objective To investigate the probability and timing of childhood Miller-Fisher syndrome (MFS) progressing to Bickerstaff brainstem encephalitis (BBE), classical Guillain-Barre syndrome (GBS), and pharyngeal-cervical-brachial (PCB-GBS). Methods The clinical data of 128 children with confirmed MFS diagnosis were retrospectively analyzed. Results Among 128 children, 60 cases were simple MFS (ocular muscle paralysis, ataxia, reflexes diminished or disappeared, without limbs weakness and lethargy; laboratory tests suggest cerebrospinal fluid protein-cell separation and/or serum anti-GQ1b antibody positive), 28 cases developed MFS/PCB-GBS (met MFS diagnosis criteria, accompanied by weakness of pharynx, neck and upper limb, weakened or disappeared of upper limb reflex, without weakness of lower limb), 22 cases developed MFS/GBS (met MFS diagnosis criteria, accompanied by weakness of limb), 18 cases developed MFS/BBE (met MFS diagnosis criteria, accompanied by lethargy, pyramidal tract positive). There were no differences in the age at onset, the interval from onset to the start of the treatment, Hughes functional grading, and the percentage of cases having a history of preceding infections, the rate of positive serum anti-GQ1b antibody, the ratio of albumin cytological dissociation in cerebrospinal fluid among 4 groups (P>0.05). The interval from MFS onset to progression to MFS/PCB-GBS, MFS/GBS, or MFS/BBE was within 10 days. Conclusions In children with MFS, 50% developed PCB-GBS, GBS, or BBE, which occurred within 10 days after onset. Clinicians should pay attention to the time window and adjust the medicine rationally.

With clinical medical professional certification as a background and combining Guangxi Medical University Teaching Quality Monitoring System of practical experiences,the author pointed out that internal teaching quality monitoring system problems during the operation,discussed the constraints of issues affecting the effective functioning of the teaching quality monitoring,and proposed corresponding suggestions.

Objective: To investigate the mechanism of Shenyankang(肾炎康) on rats with mesangial proliferative glomerulonephritis(MsPGN).Methods: MsPGN rat model was induced by chronic(serum-sickness).Six weeks later,MsPGN rats were given 3 ml of Shenyankang oral liquor every day(Shenyankang oral liquor treated group) or 3 ml of water every day(model group).Forty-two days after(using) the drug,urine protein(UP) of 24 hours,renal function and urine level of tumor necrosis factor?(TNF?) and endothelin1(ET1) were evaluated.Pathologic changes of renal tissue were observed by microscopy.Results: Compared to the normal control group,UP of 24 hours,blood urea nitrogen(BUN), serum creatinine(SCr) and urine level of TNF? and ET1 increased significantly in model group((all P

Objective To investigate the relationship between the different deep venous drainage patterns in the brain and the perimesencephalic subarachnoid hemorrhage (PMSAH).Methods From January 2014 to January 2017,the clinical data of 90 patients with subarachnoid hemorrhage (SAH) diagnosed and treated in the Second Affiliated Hospital of Anhui Medical University were analyzed retrospectively.Thirty patients with PMSAH were in a PMSAH group and 60 patients with aneurismal SAH were in a control group.Unilateral cerebral hemisphere venous drainage was divided into type A (normal continuous):the basilar vein had deep middle cerebral vein drainage and was drained into the great cerebral vein of Galen;type B (normal discontinuous):there was discontinuous venous drainage between the basal vein and the anterior uncal vein and the posterior Galen vein;type C (primitive variant):did not drained into great cerebral vein of Galen,perimesencephalic vein was drained into the superior petrosal sinus or basal vein was directly drained into the transverse sinus or straight sinus.The different combinations of bilateral cerebral hemisphere venous drainage were divided into normal type drainage (typeⅠ:AA),discontinuous type drainage (types Ⅱ:AB or BB),and primitive type drainage (types Ⅲ:AC,BC,or CC).The differences of venous drainage between the two groups were compared.Results In the PMSAH group,both types Ⅰ and Ⅱ drainages accounted for 26.7％ (n=8 in each type) and type Ⅲ accounted for 46.7％ (n=14).In the control group,typeⅠaccounted for 48.3％ (n=29),type Ⅱ accounted for 28.3％ (n=17),and type Ⅲ accounted for 23.3％ (n=14).There were no significant differences in the distribution of three venous drainage patterns between the two groups (χ2=5.804,P=0.055).However,there was significant difference in the types Ⅲ venous drainage between the two groups (χ2=5.081,P=0.024).Conclusion Most of the deep cerebral venous drainage in patients with PMSAH showed basilar venous drainage into the venous sinuses of dura mater,but not to the large cerebral vein drainage,suggesting the way of primitive drainage into the dural sinus was more prone to rupture compared with that of drainage into large cerebral veins.

Objective:To study the effect of subepithelial connective tissue grafting in covering an exposed root surface.Methods:Eight cases with gingival recession on upper and lower anterior teeth and premolars receited connective tissue grafting received connective tissue grafting were presented with 12 months of follow-up.Results:Initial gingival recession averaged 4.3 mm,with a mean probing pocket depth of 2.3 mm.At the 12-month follow-up,a mean of 3.0 mm of root coverage was observed(ie,71% root coverage,range 60% to 80%).Probing pocket depth averaged 1.6 mm,indicating a 3.6 mm gain of clinical attachment(range 2 to 5 mm).Conclusion:The application of connective tissue graft results in predictable root coverage and clinical attachment while maintaining shallow pockets.