1.Biomarkers for diabetes prediction, diagnosis and personalized therapy.
Chinese Medical Journal 2012;125(23):4163-4166
2.Removal of NH_3-N by Microbe Without Organic Carbon Source under Oxygen Limited Condition
Microbiology 1992;0(01):-
It was investigated by ~(15)N tracer that four strains capable of ammonia oxidation under the condition of limited dissolved oxygen(DO)and without organic carbon source in a sealed bio-membrane reactor.Each strain was cultured at room temperature,a sealed vessel filled with argon and an appropriate quantity of oxygen.When the condition kept DO
3.Advances in research on the association between single nucleotide polymorphisms of the DNA repair genes and resistance to platinum-based chemotherapy.
Jia WEI ; Bao-rui LIU ; Ya-ping WANG
Chinese Journal of Oncology 2006;28(3):161-163
DNA Repair
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genetics
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DNA-Binding Proteins
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genetics
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Drug Resistance, Multiple
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Drug Resistance, Neoplasm
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Endonucleases
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genetics
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Humans
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Neoplasms
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drug therapy
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genetics
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Nuclear Proteins
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genetics
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Organoplatinum Compounds
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therapeutic use
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Platinum Compounds
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therapeutic use
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Polymorphism, Single Nucleotide
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Transcription Factors
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genetics
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X-ray Repair Cross Complementing Protein 1
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Xeroderma Pigmentosum Group D Protein
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genetics
4.Research on Collateral-unblocking Medicines for Cardiovascular Diseases under the Guidance of Vessels Collateral Theory.
Cong WEI ; Li-ping CHANG ; Zhen-hua JIA
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1513-1516
"Vessels Collateral Theory", as the inherit and development of "blood vessels" in Huangdi Neijing, is a systematic system by integrating severe vascular diseases. In this article, by taking cardiovascular diseases (CVD) as a cut-in point, roles of "minute collateral-microvascular" lesions in the occurrence and development of CVD were further explored. The interventional effect of collateral-unblocking medicines under the guidance of Vessels Collateral Theory was also in-depth explored, hoping to leading Chinese medical prevention and treatment of CVD.
Cardiovascular Diseases
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drug therapy
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Humans
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Medicine, Chinese Traditional
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Research
5.Effect of short-term treatment with metformin on psychological distress and metabolic feature in patients with polycystic ovary syndrome
Tao TAO ; Wei LIU ; Jialiang MAO ; Yun JIA ; Ping ZHANG
Chinese Journal of Endocrinology and Metabolism 2013;(3):211-215
Objective To observe the effect of short-term treatment with metfbrmin on psychological distress and metabolic feature in patients with polycystic ovary syndrome(PCOS).Methods Ninety women were diagnosed as cases of PCOS based on the 2003 Rotterdam criteria.These patients were divided into three groups:group A,consisted of 26 subjects treated with mefformin only; group B,36 cases treated with metformin plus Dane-35 ; and group C,28 cases treated with placebo and Dane-35.The treatment was carried out for 3 months.Clinical and metabolic parameters were observed.The psychological distress was evaluated by the hospital anxiety and depression scale(HAD scale).Results Compared with group C,patients in group A and B showed significantly lower body mass index(BMI),waist circumference (WC),waist-hip ratio (WHR),serum fasting insulin,homeostasis model assessment for insulin resistance (HOMA-IR),total cholesterol (TC),triglyceride (TG),low density lipoproteincholesterol(LDL-C) levels as well as anxiety and depression scores,but higher high density lipoprotein-cholesterol (HDL-C) levels (all P<0.05).Compared with group A,patients in Group B had significantly higher WC,WHR,and LDL-C levels(all P<O.05).No significant differences in BMI,TG,HDL-C,anxiety and depression scores were found between group A and B (all P>0.05).In group A,a positive correlation was found between the changes of metabolic feature (BMI,WHR,and HOMA-IR) and the changes of anxiety and depression scores (all P <0.05).Conclusion Short-term treatment with metformin will benefit patients with polycystic ovary syndrome,regarding psychological distress and metabolic features.
6.Simultaneous Determination of Total Content of p-Hydroxybenzoic Acid plus o-Hydroxybenzoic Acid and p-Hydroxybenzeneacetic Acid in Senecio Scandens Buc
Zuojun WANG ; Ping WEI ; Hui JIA ; Guobing SHI
China Pharmacist 2014;(5):767-769
Objective: To set up an HPLC method for the simultaneous determination of p-hydroxybenzoic acid plus o-hydroxy-benzoic acid and p-hydroxybenzeneacetic acid in Senecio scandens Buch. Methods:The column was a Shiseido( Fine Chemicals) Cap-cell Pak C18 (250 mm × 4. 6 mm, 5 μm) column at the room temperature. The mobile phase was methanol-water-formic acid (13∶87∶0. 5) at a flow rate of 1. 0 ml·min-1 . The detection wavelength was 240nm. Results: p-Hydroxybenzoic acid plus o-hydroxybenzoic acid had a favorable linear relationship within the range of 0. 025-0. 400 mg·ml-1 , the regression equation was Y=5. 94 × 106 X+2.46×104(r=0.9998),theaveragerecoverywas97.59% andRSDwas1.22%. p-Hydroxybenzeneaceticacidhadafavorableline-ar relationship within the range 0.05-0.80 mg·ml-1, the regression equation was Y=4.09 ×106X+1.12 ×104(r=0.999 8), the average recovery was 98. 07% and RSD was 1. 90%. Conclusion:The method is simple, feasible and reproducible. It can be used in the quality control of p-hydroxybenzoic acid plus o-hydroxybenzoic acid and p-hydroxybenzeneacetic acid in Senecio scandens Buch.
8.Inhibitory effects of PTEN gene transfection combined with L-OHP on proliferation of human cholangiocarcinoma cells
Ping CUI ; Tide DUAN ; Jian DONG ; Wei JIA ; Shupeng DAI
Chinese Journal of Cancer Biotherapy 1995;0(03):-
Objective: To investigate the inhibitory effects of PTEN gene transfection combined with L-OHP on human cholangiocarcinoma cell line, QBC939, providing a new method for gene therapy of human biliary duct carcinoma. Methods: A eukaryotic expression vector containing PTEN gene was transfected into human QBC939 cells under mediation of lipofectamine and positive cell clones were selected and amplified. Expression of PTEN gene was detected by immunohistochemistry. MTT test was used to determine the in vitro activity of cells, electron microscope was applied to observe cell ultrastructure, and flow cytometry was used for determining the cell cycle and apoptosis. In vitro test was used to study the invasive ability of cells before and after treatment. Results: After transfected with PTEN gene, QBC939 cells had a higher expression of PTEN gene (P
9.Application of glucose clamp technique in the study of diabetes mellitus
Lei CHEN ; Wei-Ping JIA ; Kun-San XIANG ;
Chinese Journal of Endocrinology and Metabolism 1985;0(01):-
The article introduces the applic at ion of glucose clamp technique in the study of diabetes. The changes in insulin sensitivity, insulin secretion, metabolism of glucose, lipids and protein, etc. in euglycemic or hyperglycemic status in vivo can be investigated by glucose cla mp technique.
10.Primary central nervous system lymphoma:report of 43 cases
Jia-Ling WU ; Zhong-Ping AN ; Wei-Yiag DAI
Chinese Journal of Neurology 2001;0(01):-
Objective To explore the clinical characteristics,therapy and prognosis of primary central nervous system lymphoma (PCNSL).Methods Forty-three PCNSL patients were retrospectively analyzed.Results The most frequent PCNSL clinical presentations were an increased intracranial pressure, focal neurologic deficits.The median survival time of patients was 18.0 months (14.2—21.8 months,95% CI).Cox regression model analysis revealed that ependymal and leptomeningeal involvement was the only independent factor for overall survival (P=0.002).Conclusions PCNSL is a aggressive disease with a poor prognosis and difficult to diagnose before operation.The optimal treatment modality for newly diagnosed PCNSL is still under investigation.