Objective:To investigate the antifungal activity of the fern species Lygodium venustum (L.venustum) and Pityrogramma calomelanos (P.calomelanos) against Candida albicans and Candida tropicalis strains.Methods:The microdilution method was used to evaluate the antifungal activity,as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole.The minimum inhibitory concentration (MIC),minimum fungicide concentration and morphological changes were also determined.Results:The extract obtained from L.venustum presented a MIC ＞ 8192 μtg/mL,while the extract obtained from and P.calomelanos presented a MIC =8192 μtg/mL,indicating that they present weak antifungal activity.However,combination of the extracts with Fluconazole potentiated the antifungal activity of this drug.At different experimental conditions,such as concentration of the extract and type of strain,the extracts inhibited hyphae and pseudohyphae formation,indicating that these fern species can affect the morphology of the fungi.Conclusions:The extracts obtained from the fern species L.venustum and P.calomelanos dose not present significant antifungal activity.However,P.calomelanos potentiates the activity of fluconazole and both extracts inhibits the morphological changes in Candida species,indicating that they have potential pharmacological activity as modulators of fungal biology.Therefore,novel studies are required to characterize the interference of these extracts in the virulence and pathogenicity of Candida species as well as the potential of fern species to treat fungal infections.