OBJECTIVE: To improve diagnosis and treatment level of foreign body ingestion of button cell in children. METHOD: Among the 14 cases with foreign bodies ingestion of button cell, 6 cases in esophageal, 7 cases in stomach and lower gastrointestinal tract, 1 case with multiple foreign bodies both in esophagus and stomach. Seven cases in stomach and lower gastrointestinal tract were closely observed without special treatment, 6 cases in esophageal, underwent esophagoscopy and removal of foreign body under general anesthesia, 1 case with multiple foreign bodies in esophagus and stomach underwent esophagoscopy and electronic gastroscopy and then removal of foreign body. All patients had symptomatic treatments after removal of foreign body. The average age is 2 years and 1 month. RESULT: After the removal of button cell, Foreign bodies in stomach and lower gastrointestinal tract in 7 cases were all discharged spontaneously. For the 6 cases in esophagus, 4 cases were cured, 1 case had esophageal stricture, 1 cased died. 1 case with multiple foreign bodies in both esophagus and stomach was cured. CONCLUSION Esophageal foreign body has strong corrosiveness and would cause serious damages even to threaten children's life. It also may lead to esophageal stricture. The key point to cure this disease is to diagnose timely and to operate as soon as possible. Because neutralize of digestive juices and low probability of incarceration, with close observation, the foreign bodies of stomach and lower gastrointestinal tract can discharge spontaneously.
Child, Preschool ; Esophagus ; Female ; Foreign Bodies ; etiology ; Humans ; Infant ; Male ; Stomach

ABSTRACT:OBJECTIVE Toxicity model of zebrafish was used to evaluate safety of 26 kinds of common orthopedic herbal medicine.METHODS Zebrafish embryo at 1 day post fertilization(dpf)were exposed with various concentration of water de-coction;the death number of the embryos or larvals was counted from 1dpf to 6 dpf;embryonic micro-morphology of zebrafish (3dpf) was observed and pictures were taken that compared with 0.4% DMSO;LC50 value at 6dpf was calculated by SPSS1 6. 0.RESULTS The results indicated that 13 kinds of water decoction of herbal medicines(Dipsaci Radix,Anemarrhena Rhizo-ma,Olibanum and Myrrha,Psoraleae Fructus,Angelicae Pubescentis Radix,Achyranthis Bidentatae Radix,Polygoni multi-flori Radix,Cnidii Fructus,Aucklandiae Radix,Eupolyphaga Steleophaga,Epimedii Folium and Acanthopanacis Cortex) can cause obvious organ toxicity to juvenile zebrafish,such as yolk sac swelling,deformation,black,pericardial edema and bleed-ing,and their LC50 values were low(1 6.9 ~ 689.7 μg∕mL),while the other 13 herbal medicines are relatively safe (Pyrolae Herba,Homalomena Rhizoma,Sappan Lignum,Morindae Officinalis Radix,Testudinis Carapax Et Plastrum,Drynariae Rhi-zoma,Salviae miltiorrhizae Radix Et Rhizoma,Cibotii Rhizoma,Taxilli Herba,spatholobi CauLis,Rehmanniae Radix Pra-eparata,Ligustri Lucidi Fructus and Eucommiae Cortex),no fish organ toxicity was observed,and their concentration caused zebrafish death was usually at high levels (above 2 000 μg∕mL).CONCLUSION Zebrafish model was successfully used for screening toxicity of 26 common orthopedic herbal medicine,which may provide useful information for reasonable application of them.Zebrafish model has advantages of simplicity,high efficiency and real-time observation,which made large scale toxicity screening of herbal medicine in vivo style possible.

ABSTRACT:OBJECTIVE To study the effect of isoliquiritigenin on cell proliferation,apoptosis induction,mRNA expression of endoplasmic reticulum stress related gene ATF4,DDIT3 and TNFRSF10B in NCI-H1 57 human lung cancer cell line. METHODS NCI-H1 57 cells were cultured and treated with various concentrations isoliquiritigenin.The cell proliferation was analyzed by MTT and the occurrence of cell apoptosis was examined by flow cytometry.ATF4,DDIT3 and TNFRSF10B gene mRNA expression were detected by real-time fluorescence quantitative polymerase chain reaction.RESULTS Isoliquiritigenin significantly inhibited NCI-H1 57 cell proliferation in a dose and time dependent manner.The results of flow cytometry revealed that isoliquiritigenin could induce apoptosis in NCI-H1 57 cells.Real-time quantitative RT-PCR showed that isoliquiritigenin up-regulated mRNA expression of ATF4,DDIT3 and TNFRSF10B(P <0.05 ~0.01).CONCLUSION Isoliquiritigenin can in-hibit NCI-H1 57 cell proliferation,induce cell apoptosis by activating the endoplasmic reticulum stress response.

OBJECTIVE To investigate the function of JAK2/STAT3 pathway in promoting angiogenesis with Angelica mi-crocapsules for replenishing blood (Ang-Mc).METHODS Establish the human umbilical vein endothelial cell (HUVEC) model,and then cell counting kit(CCK-8) and flow cytometry were used to test cell viability and cell apoptosis.Western blot was used to detect expression of p-STAT3 and VEGF.RT-PCR was used to detect expression of vascular endothelial growth factor(VEGF) and miRNA-21.RESULTS When Ang-Mc with a concentration of 20 μg/mL acted on HUVEC for 24 h,it could effectively promote the HUVEC proliferation by up-regulating the expression of p-STAT3,VEGF and miRNA-21. AG490 can effectively inhibit Ang-Mc promoting HUVEC proliferation effect and reduce the expression of p-STAT3,VEGF and miRNA-21.CONCLUSION The effect of Ang-Mc in promoting angiogenesis is related to JAK2/STAT3 signaling path-ways.

OBJECTIVE A comprehensive understanding of the major and minor elements in different processed products of Pyrite,providing scientific basis for the clinical rational use of Pyrite.METHODS The determination method of 10 compo-nents including Fe,S,Si,Al and other major and minor components in different processed products of Pyrite by X-ray fluores-cence spectrometry was established.RESULTS The detection limit of Fe,S,Mn,Si,Al,Ti,Ca,P,Zn and Pb was 13.37, 36.92,7.56,68,29,77.47,12.46,22.54,21.64,4.55,3.24 mg/kg.Determination of standard material composition of GBW(E)070088 (iron ore)and GBW 07267 (pyrite)for 5 repetitions.The accuracy were 94%~107% and the RSD were 1. 0%~10.1%.CONCLUSION The method can be used to determine the major and minor elements in different processed prod-ucts of Pyrite,and it is accurate and convenient.

OBJECTIVE To explore the influence of different compatibility of Shuangshen Pingfei decoction on the decocting rate of mangiferin in Anemarrhena asphodeloides ,and ingredients to research influence on the mechanism of the solution rate. METHODS To disassemble formulas on the basis of the prescription of traditional Chinese medicine containing categories of ingredients.With decocting rate of mangiferin in Anemarrhena asphodeloides Bge as indexes,analyse different compatibility influence on mangiferin decocting rate.Based on these results of disassembled formulas,the representative alkaloid 1-Deoxyno-jirimycinand amino acid in different compatibility are used to research the influence of compositions of white mulberry on the decocting rate of mangiferin in Anemarrhena asphodeloides Bge and discuss the influence of the decocting rate mechanism with Anion and cation exchange resin processing of samples.RESULTS Research findings shows that the decocting rates of man-giferin in Anemarrhena asphodeloides Bge decoction,prescription combined decoction,Anemarrhena asphodeloides Bge-sapo-nins from Chinese herbal compound,Anemarrhena asphodeloides Bge-phenolic acid from Chinese herbal compound are 97.33%,47.03%,94.08%,57.53%,34.31%,respectively;Further study shows that Morus alba L.combined with Anemarrhena asphodeloides Bge have the most obvious effect on extraction yield of mangiferin.Anemarrhena asphodeloides Bge decoting with total alkaloids of Morus alba L,DNJ,glycine,β-alanine,respectively.The decocting rate of mangiferin in each group was 1 6.64%,28.13%,40.75% and 48.56%,respectively.CONCLUSION Our results suggest that the decoc-ting rates of mangiferin are mainly influenced by Morus alba L.We also conjectured that alkaloids and amino acids of Morus alba L.form complexes with mangiferin.