BACKGROUND: The functional MRI(fMRI),a harmless research method,has brought new perspective to the understanding of ancient needling methods. The correlation between meridian points and cerebrum has been studied with fMRI in different cerebral regions.OBJECTIVE: To observe the correlation between Kuangming( Luo-Connection Point,GB37)and Taichong(Shu-Stream and Yuan-Source point,Liv3)with fMRI in different cerebral regions.DESIGN: Randomized controlled experimental study based on healthy volunteers of medical college.SETTING: Department of acupuncture in a medical university of traditional Chinese medicine,basic medical college in a medical university of traditional Chinese medicine,medical radiology center of medical college of University Freiburg in Germany.PARTICIPANTS: This study was conducted in Medical Radiology Center of Medical College of University Freiburg in Germany in June 1997. In this study,altogether 19 healthy volunteers,4 males and 15 females with normal vision,were selected. All of them were medical students without any previous acupuncture experience and history of neurology and psychosis. All healthy volunteers were randomly divided into three groups,including visual stimulation + one- sided needling group( n =7,2 males and 5 females),visual stimulation + double-sided needling group (n = 6,1 male and 5 females)and only double-sided needling group( n = 6,1 male and 5 females).METHODS: The response of visual cortex and the relative areas,and whether there comes inducing function were observed by fMRI during puncturing at kuangming and taichong. All subjects were informed about the range of possible acupuncture sensation they might experience during needle manipulation before entering the magnet field. For acupuncture,a stainless immagnet acupuncture needle(with a diameter of 0.25 mm and a length of 30 mm. Huatuo Brand. Made in Suzhou Medical Inc. ) was used. All subjects were performed by one experienced acupuncturist.MAIN OUTCOME MEASURES: The changes,extent,delayed response,and time fluctuation of the cerebral cortex hemoglobin saturation level(BOLD).Whether the cerebral visual cortex domain was activated or not.RESULTS: There were no change of BOLD in visual cortex during visual stimulation and inserting needle at Kuangming and Taichong in visual stimulation +single sided needling group and visual stimulation +double-sided needling group. There were changes of BOLD of visual cortex in all subjects of the three groups during the period of manipulating needle. Among them six cases were found to have in the insular lobe,temporo-inferior parietal lobule,superior colliculus lobe,cuneate lobe and occipital mid-gyri.CONCLUSION: Single-sided or double-sided continuous needle stimulation can change the BOLD of the related regions of the cerebrum,but the change is unrelated with the insertion method and visual stimulation.

OBJECTIVESTo evaluate the effects of 19-nortestosterone (NT) on the growth and development of the ventral prostate (VP), epididymis, and seminal vesicles (SV) in hypogonadal (hpg) mice.

METHODSThe silastic tube filled with NT was implanted subdermally into mature hpg mice (n = 7) for five weeks. Similar silastic tubes without NT were implanted into both of hpg mouse control group (n = 7) and normal mouse group (n = 10) instead. The weights of sex accessory glands and the branch tip number of VP from all mice were evaluated.

RESULTSThe weights of VP, SV, and epididymis in NT treated hpg group were significantly higher than those of hpg control group (P < 0.005); and the branching morphology of the VP showed a tendency to be normal and the development of prostate ductal tip was improved significantly. Especially, the weight of SV in NT treated hpg mice was equal to that of normal mice, while the weights of VP, epididymis and branching tip number in NT treated hpg group was still significantly lower than that of normal mice (P < 0.005).

CONCLUSIONSThe NT treatment significantly stimulates the growth and development of the sex accessory gland in mature hpg mouse.

Animals ; Epididymis ; drug effects ; growth & development ; Genitalia, Male ; drug effects ; growth & development ; Hypogonadism ; drug therapy ; physiopathology ; Male ; Mice ; Nandrolone ; therapeutic use ; Prostate ; drug effects ; growth & development ; Seminal Vesicles ; drug effects ; growth & development

Toxoplasmosis is caused by the intracellular protozoan Toxoplasma gondii. It is anopportunistic zoonosis in warm-blooded animals and humans, with a worldwide distribution. Toxoplasma gondii dense granule protein 16 (TgGRA16) can modulate some functions in host cells and is considered a significant virulent factor of the parasite. The present study reports sequence variation in TgGRA16 gene among T. gondii strains from different hosts and geographical locations, and the construction of phylogenetic relationships of these T. gondii strains based on sequences of TgGRA16, and analysis of B cell epitopes in TgGRA16. Our results showed that all TgGRA16 gene sequences were 1518 bp and the C+G contents ranged from 52.17% to 52.59%. Sequence variation in the TgGRA16 gene was 0-1.51%. Phylogenetic analysis revealed that TgGRA16 gene sequence could not be used to differentiate the different T. gondii genotypes. Six B cell epitopes were predicted in TgGRA16. These results indicated that TgGRA16 gene is not an ideal marker for studying genetic relationships of T. gondii isolates, but may represent a good vaccine candidate against toxoplasmosis.

Objective: To understand the characteristics of HIV infected persons without long term disease progress [also known as long term non-progressors (LTNPs)], and related factors in Guangxi Zhuang Autonomous Region (Guangxi). Methods: Data of persons living with HIV and receiving no antiretroviral therapy in Guangxi by the end of 2016 were collected from the national HIV/AIDS comprehensive control and prevention information system of China. Results: By the end of 2016, there were 313 LTNPs in Guangxi, accounting for 2.3% of those being reported for more than 10 years, 5.4% of those being reported for more than 10 years and surviving, and 26.6% of those being reported for more than 10 years, surviving and receiving no antiretroviral therapy. Among the LTNPs, 87.2%(273) were men, 94.9% (297) were aged ≤ 40 years, 32.3% (101) were farmers, 55.6% (174) were single, divorced or widowed, 69.3% (217) were of Han ethnic group, 68.1% (213) were injecting drug users, and 52.1% (163) were from custody facilities. Multiple logistic regression analysis indicated that factors associated with delayed disease progression included age ≤40 years (compared with age >40 years, aOR=1.55, 95%CI: 1.31-3.12) and injection drug use (compared with sexual transmission, aOR=1.23, 95%CI: 1.10-1.74). Conclusions: A number of LTNPs existed in HIV-infected individuals in Guangxi. Further research are needed to identify the related factors, and it is necessary to conduct large sample size studies on host immunology, genetics and the virology of HIV to explore the related mechanism.
Adolescent ; Adult ; Age Distribution ; China/epidemiology* ; Drug Users/statistics & numerical data* ; Ethnicity/statistics & numerical data* ; HIV Infections/ethnology* ; Humans ; Male ; Socioeconomic Factors

OBJECTIVETo obtain the polymorphic data of short tandem repeat(STR) loci of D15S657, D11S1369, D6S2420 and D6S503 in Chinese Han population and to study the association of these four STR loci with insulin-dependent diabetes mellitus(IDDM).

METHODSThe polymorphisms of the four STRs were studied by polymerase chain reaction-polyacrylamide gel electrophoresis(PCR-PAGE) followed by direct sequencing of PCR products in 105 normal Chinese Hans and 48 patients with IDDM.

RESULTSSeven alleles at D15S657 locus, 5 alleles at D11S1369 locus, 7 alleles at D6S2420 locus and 4 alleles at D6S503 locus were found. No deviation from Hardy-Weinberg equilibrium was observed. The heterozygosities of these loci were 0. 7524, 0.6000, 0.6286, 0.6571 and the polymorphic information contents(PIC) 0.7616, 0.4430, 0.5345 and 0.5932, respectively. The allele frequencies of allele A(5) at D15S657 locus, allele A(5) at D11S1369 locus and allele A(4) at D6S2420 locus were increased significantly in patients with IDDM, compared to those in the control group.

CONCLUSIONThe four STRs, used as genetic markers, were suitable for case-control study, forensic medicine identification and population genetic study. There is an association between the polymorphisms of D15S657, D11S1369, D6S2420 and IDDM.

Adult ; Aged ; China ; ethnology ; Diabetes Mellitus, Type 1 ; genetics ; Female ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Tandem Repeat Sequences

Objective: To understand prevalence and transmission of transmitted drug resistance (TDR) among HIV infected men who have sex with men (MSM) in Tianjin from 2014 to 2017. Methods: A total of 225 blood samples were collected from HIV infected MSM in Tianjin from 2014 to 2017. Pol gene fragments were obtained by viral RNA extraction and nested PCR amplification. Phylogenetic and drug resistance analyses were conducted. Results: A total of 205 samples were successfully sequenced and analyzed. Based on pol sequences, 53.2% (109/205), 28.8% (59/205), 10.2% (21/205), 4.9% (10/205) and 2.9% (6/205) of the samples were positive for HIV subtypes CRF01_AE, CRF07_BC, B, CRF55_01B and unique recombinant forms (URFs). Twenty transmission clusters, including 75 sequences, were identified and 62.5% (10/16) of sequences with TDR were in 5 clusters. The prevalence of TDR was 7.8% between 2014 and 2017. The annual prevalence rate increased from 3.9% (2/51) in 2014, 5.7% (3/53) in 2015, 9.6% (5/52) in 2016 to 12.2%(6/49) in 2017, the difference was not significant (χ(2)=2.504, P=0.127). CRF01_AE and B strains had high TDR prevalence (3.4%, 7/205) and (2.9%, 6/205), respectively. The TDR mutation was mainly NNRTIs, the TDR prevalence was 6.3% (13/205). In contract, the TDR prevalence of NRTIs and PIs were 1.5% (3/205) and 1.0% (2/205) respectively. Conclusion: Results from this study suggested that the prevalence of HIV-1 TDR strains in MSM was serious in Tianjin. It is necessary to take effective prevention and control measures.
China ; Drug Resistance, Viral/genetics* ; Genes, pol ; Genotype ; HIV Infections/transmission* ; HIV Reverse Transcriptase/genetics* ; HIV Seropositivity/genetics* ; HIV-1/isolation & purification* ; Homosexuality, Male/statistics & numerical data* ; Humans ; Male ; Mutation ; Phylogeny ; Polymerase Chain Reaction ; Prevalence ; RNA, Viral/genetics* ; pol Gene Products, Human Immunodeficiency Virus/genetics*

Immunoglobulin A vasculitis (IgAV), also known as Henoch-Schönlein purpura, has complex etiology and pathogenesis which have not been fully clarified. The latest research shows that SARS-CoV-2 and related vaccines, human papilloma vaccine, and certain biological agents can also induce IgAV. Most studies believe that the formation of galactose-deficient IgA1 (Gd-IgA1) and Gd-IgA1-containing immune complex plays a crucial role in the pathogenesis of IgAV. It is hypothesized that the pathogenesis of IgAV is associated with the binding of IgA1 to anti-endothelial cell antibodies. In addition, genetics also constitutes a major focus of IgAV research. This article reviews the new advances in the etiology of IgAV and summarizes the role of Gd-IgA1, Gd-IgA1-containing immune complex, anti-endothelial antibody, IgA1 conjugates, T lymphocyte immunity, and genetic factors in the pathogenesis of IgAV.
Humans ; IgA Vasculitis ; Antigen-Antibody Complex ; Immunoglobulin A/genetics*

OBJECTIVETo observe the clinical efficacy on primary dysmenorrhea treated with isolated herbal moxibustion on Shenque (CV 8).

METHODSOne hundred and two cases were randomized into isolated herbal moxibustion group and western medication group, 51 cases in each one. In isolated herbal moxibustion group, the cases were treated with isolated herbal moxibustion on Shenque (CV 8), and in western medication group, analgesic was administered.

RESULTSIn isolated herbal moxibustion group, 17 cases were cured, 21 cases effective markedly, 9 cases effective and 4 cases failed. The total effective rate was 92.2%. In western medication group, 7 cases were cured, 11 cases effective markedly, 21 cases effective and 12 cases failed. The total effective rate was 76.5%. The comparison of the total effective rates between two groups indicated significant difference in statistics (P < 0.01), in which, the result in isolated herbal moxibustion group was superior to that in western medication group. In comparison of dysmenorrhea score before and after treatment and side effects of two groups, the significant differences presented in statistics, in which, the results in isolated herbal moxibustion group were superior to those in western medication group (all P < 0.01).

CONCLUSIONThe isolated herbal moxibustion on Shenque (CV 8) is superior to oral medication of analgesic for primary dysmenorrhea and it deserves to be promoted due to its advantages on safety, less side effect and good compliance of patients.

Acupuncture Points ; Adolescent ; Adult ; Dysmenorrhea ; therapy ; Female ; Humans ; Moxibustion ; Young Adult

OBJECTIVETo evaluate the efficacy of laparoscopic sleeve gastrectomy(LSG) on improving glycemic control of morbidly obese patients with type 2 diabetes mellitus.

METHODSProspective study of 30 type 2 diabetes mellitus(T2DM) patients with BMI > or = 35 undergone LSG was carried out. Pre- and post-operative clinical parameters associated with diabetes mellitus 6 months after operation were evaluated. Data collected included demographics, weight loss(BMI, EWL%), diabetes control (FPG, HbA1C) and ghrelin.

RESULTSFasting plasma glucose and glycosylated hemoglobin concentration tests and clinical outcomes suggested LSG resulted in significant improvement or resolution of T2DM in all the 30 patients with CR 63%(19/30) and PR 37%(11/30).

CONCLUSIONSLSG can lead to significant changes in controlling glycemia on morbidly obese patients with T2DM in 6 months. Long-term efficacy needs further follow-up.

Adult ; Aged ; Diabetes Mellitus, Type 2 ; complications ; surgery ; Female ; Gastrectomy ; methods ; Humans ; Laparoscopy ; Male ; Middle Aged ; Obesity, Morbid ; complications ; surgery ; Prospective Studies ; Treatment Outcome ; Young Adult