Objective To analyze the concomitant malforrnations,chromosomal abnormalities and outcomes in prenatally diagnosed congenital diaphragmatic hernia (CDH) cases. Methods Cases of fetal CDH,prenatally identified in the First Affiliated Hospital of Sun Yat-sen University from January 2002 to November 2008,were recruited.The concomitant realformations,chromosomal abnormalities and outcomes of fetal CDlH were analyzed.Fisher's exact test was applied. Results During the study period,71 CDH cases were identified including 62(87.3%) left-sided CDH and 9 (12.7%) right-sided ones.Among the 71 CDH fetuses,38(53.5%)were isolated CDH.33 (46.5%)were complicated with other realformations(complex CDH),including 18(54.5 0A) cardiovascular defects,10 (30.3%)central nervous system abnormalities,9(27.2 0A)genitourinary abnormalities and others.Fetal karyotying was performed in 19 out of the 71 CDH fetuses.among which 12 were isolated CDH cases with normal karyotype,and 4 of the rest 7(4/7)complex CDH cases with chromosomal abnormalities showing a significant differenee compared to the isolated CDH (P.0.009).Sixty-five pregnancies were terminated including all complex CDH(n=33)and 32 isolated CDH.The rest 6 isolated CDH fetuses were term delivered and 5 of them survived after repair of diaphragmatic hernia and one died after birth. Conclusions Left-sided CDH are more common than right-sided ones. Approximately half of the CDH cases are complicated with other malformations,especially cardiovascular abnormalities.The risk of chromosomal abnormalities increases in complex CDH and is relatively low in isolated CDH.The influence of surgical procedure on the prognosis of CDH has not yet determined.

AIMTo study the chemical constituents from water extract of Radix isatidis. (Isatis indigotica Fort. ).

METHODSThe water extract was underwent absorption by D101 macroporous resin, the portion eluted by ethanol of different concentrations was isolated and purified on silica gel column repeatedly. The obtained compounds were identified and structurally elucidated by their physico-chemical properties and spectral analysis.

RESULTSFive compounds were isolated from water extract of Radix isatidis, and were partly identified separately: 3-[2'-(5'-hydroxymethyl) furyl] -1 (2H) -isoquinolinone-7-O-beta-D-glucoside (I), lariciresinol-4,4'-di-O-beta-D-glucopyranoside (II), lariciresinol-4-O-beta-D-glucopyranoside (III), 2-hydroxy-1, 4-benzenedicarboxylic acid (IV), mannitol (V).

CONCLUSIONCompound I is a new compound and compounds IV and V were isolated from the plant for the first time.

Isatis ; chemistry ; Magnetic Resonance Spectroscopy ; Plant Extracts ; analysis

Objective To analyze the effect of systematic management of human milk feeding on the feed -ing of hospitalized preterm infants .Methods Preterm infants hospitalized at the neonatal intensive care unit of Peking Union Medical College Hospital from January to December 2011 and from January to December 2013 who met certain enrollment criteria were included and their clinical data were collected .The preterm infants in 2011 served as the control group , in which the parents were given routine education and infants given routine nursing . The systematic management of human milk feeding has been implemented since 2012 , including staff training , special education of the family members of preterm infants , management of human milk collection , delivery , stor-age, preparation, warming, and feeding, establishing a comprehensive plan system supporting human milk feed-ing both during hospital stay and after discharge .The preterm infants in 2013 were as the study group .The 2 groups were compared in terms of human milk feeding state , incidence of sepsis , and length of hospital stay . Results One hundred and sixteen infants were enrolled , including 54 boys and 62 girls, 53 in the study group and 63 in the control group .There were no significant inter-group differences in gender , gestational age , birth weight , and length of hospital stay ( P>0.05 ) .Compared with the control group , the study group had an earlier initiation of human milk feeding [(3.98 ±2.45) d vs.(5.05 ±1.76) d, P=0.008], higher percentages of human milk feeding in the first 7 days (42.98%vs.23.14%, P=0.001 ) and 14 days (60.27%vs.40.95%, P=0.001), a higher proportion of exclusively human milk fed infants (75.48%vs.34.92%, P=0.041), and a lower incidence of sepsis (15.1%vs.23.8%, P=0.012).Conclusions The implementation of systematic management of human milk feeding could effectively facilitate human milk feeding of hospitalized preterm infants , raise the human milk feeding rate , and reduce the incidence of nosocomial infection .

OBJECTIVETo explore the potential anti-inflammatory and analgesic activities of carboxyamidotriazole (CAI).

METHODSA variety of animal models, including the croton oil-induced ear edema, the cotton-induced granuloma, the rat adjuvant-induced arthritis, were used to evaluate anti-inflammatory effect of CAI. Vascular endothelial growth factor (VEGF)--or histamine-stimulated local vascular permeability in mouse modulated by CAI was also determined. In addition, we assessed the effect of CAI on the levels of proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-beta) at the site of inflammation and in sera. Moreover, antinociceptive effect of CAI on inflammatory pain was assessed using acetic acid-induced writhing model and the formalin test.

RESULTSCAI significantly inhibited acute and chronic phases of inflammation, reduced VEGF or histamine-induced vascular permeability, and showed marked inhibition of proinflammatory cytokines such as TNF-alpha and IL-1 beta. CAI also showed potential therapeutic effect on peripheral inflammatory pain.

CONCLUSIONCAI is a promising anti-inflammatory and analgesic agent.

Analgesics ; pharmacology ; Animals ; Anti-Inflammatory Agents ; pharmacology ; Drug Evaluation, Preclinical ; Female ; Male ; Mice ; Mice, Inbred ICR ; Rats ; Rats, Wistar ; Triazoles ; pharmacology

This study was aimed to explore the pharmacokinetics of epiberberine, jatrorrhizine, coptisine, palmatine, berberine of Jiaotai pill in the normal and depressed rats. According to ‘Katz’ method, the model of chronic unpredictable mild stress (CUMS) was established. The extract of Jiaotai pill was orally administered to rats, and the blood samples were collected via the the oculi chorioideae vein according to the time schedule. The concentrations of epiberberine, jatrorrhizine, coptisine, palmatine, berberine in rat plasma were determined by LC-MS/MS, and the pharmacokinetic parameters were calculated by DAS1.0 software. Compared with normal rats, the C_max of palmatine, coptisine, berberine and jatrorrhizine in Jiaotai pill in depressed rats were 1.99, 2.14, 2.3, 1.82 times than the normal group, while the AUC_0−t were 1.23, 1.25, 1.29, 1.46 times and the AUC_0−∞ were 1.21, 1.25, 1.30, 1.43 times, which were significantly different.