OBJECTIVEThrough analysis of variation and function of 5 main endogenous hormones in the formation of microtuber of Dioscorea opposite in vitro to explore the physiological and biochemical mechanism of microtuber development.

METHODWhen microtubers were induced on MS + 6-BA 1.5 mg x L(-1) + NAA 1.5 mg x L(-1) + sucrose 5% medium, the endogenous hormones were isolated during different formation stages of microtubers, then purified and detected with enzyme-linked immunosorbent assays (ELISA).

RESULTThe results showed that GA3 slightly decreased in initial period, rose suddenly 20 days later, and than decreased. IAA showed a dropping tendency in the total course, ABA and ZR increased in a long period, dropped at last. JA continuously rose and never dropped, GA3 and ABA and the ratio of GA3 and JA varied obviously.

CONCLUSIONIAA, ABA, JA , ZR and GA3 play an important role in controlling formation of microtubers in D. opposite in vitro.

Abscisic Acid ; metabolism ; Cyclopentanes ; metabolism ; Dioscorea ; growth & development ; metabolism ; Gibberellins ; metabolism ; Indoleacetic Acids ; metabolism ; Isopentenyladenosine ; analogs & derivatives ; metabolism ; Oxylipins ; metabolism ; Plant Growth Regulators ; metabolism ; Plant Tubers ; growth & development ; metabolism

OBJECTIVETo study the change of endogenous hormone (ABA, IAA, JA, GA3, ZR) in the leaves, petioles, tubers of Pinellia ternate around sprout tumble. It also provided some valuable information to prevent sprout tumble and increase production.

METHODTubers of P. ternata were cultured firstly at (23 +/- 1) degree C for certain days, and then they were coerced under (30 +/- 1 ) degree C stress in the same artificial climate boxes. The endogenous hormones in leaves, petioles and tubers during different stages of high temperature stress were determined with Enzyme-linked Immunosorbent Assays (ELISA).

RESULTAfter under high temperature stress, ABA content in leaves, petioles and tubers increased obviously. Similarly, JA content rose all in the leaves, petioles and tubers. But in the same conditions IAA content declined significantly in the leaves and petioles. In the tubers, IAA content also decreased, but not quickly. With the extension of high temperature coercion, the leaves, petioles, tubers, ZR content were gradually falling off. In the leaves of GA3 content rose markedly at the third day, fell down at the sixth day, but remained higher than before treatment. With the extension of the processing time, GA3 content fell off in the petioles and tubers.

CONCLUSIONABA, JA, ZT and GA3 played an important role in controlling sprout tumble of P. ternata.

Abscisic Acid ; metabolism ; Cyclopentanes ; metabolism ; Enzyme-Linked Immunosorbent Assay ; Gibberellins ; metabolism ; Hot Temperature ; Indoleacetic Acids ; metabolism ; Isopentenyladenosine ; analogs & derivatives ; metabolism ; Oxylipins ; metabolism ; Pinellia ; metabolism ; physiology ; Plant Growth Regulators ; metabolism ; Plant Leaves ; metabolism ; Plant Tubers ; metabolism ; Plants, Medicinal ; metabolism ; physiology