OBJECTIVETo study the chemical constituents of aerial part of Rabdosia serra.

METHODThe compounds were isolated by extraction, coloum chromatography over silica gel and ODS, and preparative HPLC. Their structures were identified by various spectroscopic methods including MS, IR, 1D and 2D NMR data.

RESULTSix compounds were isolated from R. serra and were characterized as ent-1alpha, 7alpha, 14beta, 20-tetrahydroxy-11, 16-kauradien-15-one (1), kamebakaurin (2), dihydrokamebakaurin (3), rabdoinflexin A (4), daucosterol( 5), and beta-sitosterol (6).

CONCLUSIONCompound 1 is a new compound, and coumpound 3 was obtained from this plant for the first time.

Isodon ; chemistry

The genus Rabdosia is famous for the abundance of diverse and novel ent-kaurane diterpenoids. However, only a few ent-kauranoids have been discovered from R. flexicaulis since the investigation on its chemical constituents is not systematic. To find novel bioactive diterpenoids, the ethyl acetate extract of the above ground part of R. flexicaulis in Daofu County, Sichuan Province was obtained by column chromatography. One new compound and five known ones were identified as flexicaulin E(1), forrestin B(2), inf-lexarabdonin D(3), 7α-hydroxydehydroabietic acid(4), 15-hydroxydehydroabietic acid(5), and pomiferin F(6) by spectral techniques. Compounds 1-3 were the ent-kaurane diterpenoids isolated from this species for the first time. Compounds 4-6, aromatic abie-tanoids, were isolated from the genus Rabdosia for the first time.
Diterpenes ; Diterpenes, Kaurane ; Isodon/chemistry* ; Molecular Structure ; Plant Extracts/chemistry*

OBJECTIVETo study the chemical constitutes in Rabdosia japonica var. galaucocalyx .

METHODThe compounds were isolated by nomal phase silica gel chromatography. The structures were identified by physical and spectral data.

RESULTNine triterpenoids were isolated and identified as friedelin (I), 3beta, 28-dihydroxy-ursane (II), ursolic acid (III), 3beta-acetyloxy-ursolic acid (IV), 2alpha, 3alpha-dihydroxy-urs-12-en-28-oic acid (V), 2alpha, 3alpha, 23-trihydroxy-urs-12-en-28-oic acid (VI), oleanic acid (VII), beta-sitosterol (VIII), daucousterol (IX).

CONCLUSIONCompounds I, II, IV, V and VI were isolated from R. japonica var. galaucocalyx for the first time.

Isodon ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Triterpenes ; chemistry ; isolation & purification

AIM: To study the chemical constituents in the flowers and fruits of Rabdosia excisa. METHODS: The compounds were isolated and purified by silica gel column chromatography and their structures were identified by spectroscopic methods. RESULTS: Sixteen compounds isolated from the flowers and fruits of this plant were identified as: stigm asterol (I), α-amyrin palmitate (II), ursolic acid (III), 2α, 3α, 19-trihydroxy-urs-12-en-28-oic acid (IV), 2α-hydroxyursolic acid (V), maslinic acid (VI), isodonal (VII), maoyecrystal E (VIII), kamebakaurin (XI), macrocalyxin G (X), epinodosinol (XI), rabdosichuanin C (XII), kamebacetal A (XIII), oridonin (XIV), enmenol-glucoside (XV), and lasiononin (XVI). CONCLUSION All the constituents were found in Rabdosia excisa for the first time, except constituents III, IX, XII and XIV.
Flowers ; chemistry ; Fruit ; chemistry ; Isodon ; chemistry ; Molecular Structure ; Plant Extracts ; chemistry

The chemical constituents of the water extraction of the aerial parts of Isodon henryi were investigated by various chromatographic methods including D-101 macroporous adsorptive resins,silica gel,sephadex LH-20,and semi-preparative HPLC. As a result,ten compounds were separated and purified. By analyses of the UV,IR,MS,NMR spectra,their structures were determined as rabdosinate( 1),lasiokaurin( 2),epinodosinol( 3),rabdosichuanin C( 4),epinodosin( 5),hebeirubescensin k( 6),rubescensin C( 7),enmenol( 8),oridonin( 9),and enmenol-1-β-glucoside( 10). Compounds 1-8 and 10 were isolated from I. henryi for the first time. Compounds 2 and 9 showed inhibitory effects against four tumor cells,with IC50 values of 2. 25-9. 32 μmol·L-1.
Isodon ; chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Phytochemicals ; analysis ; Plant Components, Aerial ; chemistry ; Plant Extracts ; chemistry

OBJECTIVETo investigate the constituents of the stem of Rabdosia excisa.

METHODCompounds were separated and purified by silica gel column chromatography and their structures were determined by spectroscopic method.

RESULTThirteen compounds isolated from this plant were identified as rabdesimte( 1 ), maoyecrystal E (2), 6beta,11alpha,15alpha-trihydroxy-6,7-seco-6,20-epoxy-1alpha, 7-ol-ide-ent-kaur-16-en (3), enmenol-glucoside (4), oridonol (5), macrocalyxin G (6), rabdosichuanin C (7), beta-sitosterol (8), ursolic acid (9), 2alpha-hydroxyursolic acid (10), maslinic acid ( 11 ), 2alpha,3alpha,19-trihydroxy-urs-12-en-28-oicacid (12), and daucosterol (13).

CONCLUSIONExcept for compounds 5, 8, 9 and 13, the remaining compounds were found in R. excisa for the first time.

Diterpenes ; chemistry ; isolation & purification ; Isodon ; chemistry ; Plant Extracts ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Triterpenes ; chemistry ; isolation & purification

An HPLC method was developed for simultaneous quantitation of rosmarinic acid, oridonin and chrysoplenetin in the aerial parts of Isodon rubescens. Samples were analyzed on an Ultimate C18 column (4.6 mm x 250 mm, 5 microm) with methanol and water containing 0.1% formic acid as mobile phases in a linear gradient mode. The flow rate was 1.0 mL x min(-1) and the temperature was set at 30 degrees C. The PDA detector wavelengths were set at 338 nm for rosmarinic and chrysoplenetin and at 242 nm for oridonin. The linear ranges were 0.222-2.78, 0.227-2.84 and 0.005-0.071 microg for rosmarinic acid, oridonin and chrysoplenetin, respectively. The average recoveries of the three constituents were 102.9% (RSD 1.9%), 99.6% (RSD 1.1%) and 102.5% (RSD 0.94%), respectively. This method was proved to be accurate and repeatable, and can be used for quality control of the aerial parts of I. rubescens.
Chromatography, High Pressure Liquid ; methods ; Cinnamates ; analysis ; Depsides ; analysis ; Diterpenes, Kaurane ; analysis ; Drugs, Chinese Herbal ; analysis ; Flavonoids ; analysis ; Isodon ; chemistry ; Plant Extracts ; analysis ; Plants, Medicinal ; chemistry

OBJECTIVETo investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism.

METHODSHL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred.

RESULTSOridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin.

CONCLUSIONSOridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.

Antineoplastic Agents, Phytogenic ; pharmacology ; Apoptosis ; drug effects ; Cell Division ; drug effects ; Diterpenes ; isolation & purification ; pharmacology ; Diterpenes, Kaurane ; HL-60 Cells ; Humans ; Isodon ; chemistry ; Plants, Medicinal ; chemistry ; Telomerase ; metabolism

OBJECTIVE: To evaluate the clinical therapeutic effect of Rabdosia rubescens aqueous extract on chronic pharyngitis and its safety. METHODS: One hundred and two patients suffered from chronic pharyngtis were randomly divided into an interventional group (n=51) and a placebo group (n=51). These volunteers were given 220 mL test materials once a day double-blindly for 30 days. All patients took routine physical examinations, and their throat symptom and pharynx signs were observed before and after the experiment. RESULTS: After the patients had consumed Rabdosia rubescens aqueous extract and the placebo for 30 days, the improvement rates of main clinical symptom and signs in the interventional group were 67.31% and 61.54%, while those in placebo group were 19.23% and 11.54%, with significant difference (P<0.05). Before the experiment, the integral of main clinical symptom and signs between the two groups did not show significant difference (P>0.05). But after 30 days, the integral of main clinical symptom and signs in the interventional group significantly decreased (P<0.05), compared with itself before the experiment and the placebo group. CONCLUSION Rabdosia rubescens aqueous extract is safe and effective for patients with chronic pharyngitis.
Adult ; Chronic Disease ; Double-Blind Method ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Isodon ; chemistry ; Male ; Middle Aged ; Pharyngitis ; drug therapy ; Phytotherapy

OBJECTIVETo study the chemical constituents of the volatile oil from the aerial parts of Isodon eriocalyx var. laxiflora.

METHODThe oil was obtained by hydrodistillation. The chemical compositions were separated and identified by GC-MS. The relative contents in the oil were determined by area normalization.

RESULT163 peaks were separated and 105 compounds were identified, constituting 85.68% of the total peak area.

CONCLUSION105 compounds characterized by GC-MS analysis were found from I. eriocalyx var. laxiflora for the first time.

Gas Chromatography-Mass Spectrometry ; Hexanols ; analysis ; isolation & purification ; Isodon ; chemistry ; Octanols ; analysis ; isolation & purification ; Oils, Volatile ; chemistry ; isolation & purification ; Phytol ; analysis ; isolation & purification ; Plant Components, Aerial ; chemistry ; Plants, Medicinal ; chemistry