1.Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G0/G1 and upregulating Bax
Wan-Ibrahim Suriyani WAN ; Ismail NORZILA ; Mohd-Salleh Farhanah SITI ; Yajid Irman AIDY ; Wong Pak-Kai MICHAEL ; Mohd Nizam Md Hashim
Asian Pacific Journal of Tropical Biomedicine 2019;9(6):249-256
Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf extracts and their effect on cell death. Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate and methanol by Soxhlet extraction. Aqueous extract was prepared by decoction at 50 ℃. Extracts of A. precatorius leaves were used to treat selected cancer and normal cell lines for 72 h. Furthermore, 3-(4,5-dimethyl thiazol-2-yl) 2,5-diphenyl tetrazolium bromide assay was performed to determine cell viability. Analysis of cell cycle arrest, apoptosis assay and apoptosis protein expressions were determined by flow cytometry. Results: Methanolic extract of A. precatorius leaves showed the lowest IC50 on MDA-MB-231 cells at (26.40±5.40) μg/mL. Flow cytometry analysis revealed that cell arrest occurred at G0/G1 phase and the apoptosis assay showed the occurrence of early apoptosis at 48 h in MDA-MB-231 cells treated with methanolic extract of A. precatorius leaves. Methanolic extract of A. precatorius leaves induced apoptosis by upregulation of Bax, p53 and caspase-3 and downregulation of Bcl-2. Conclusions: Methanolic extract of A. precatorius leaves promotes MDA-MB-231 cell death by inducing cell cycle arrest and apoptosis possibly via the mitochondrial-related pathway.
2. Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G
Wan WAN-IBRAHIM ; Norzila ISMAIL ; Siti MOHD-SALLEH ; Aidy YAJID ; Michael WONG ; Mohd MD HASHIM
Asian Pacific Journal of Tropical Biomedicine 2019;9(6):249-256
Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf extracts and their effect on cell death. Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate and methanol by Soxhlet extraction. Aqueous extract was prepared by decoction at 50 C. Extracts of A. precatorius leaves were used to treat selected cancer and normal cell lines for 72 h. Furthermore, 3-(4,5-dimethyl thiazol-2-yl) 2,5-diphenyl tetrazolium bromide assay was performed to determine cell viability. Analysis of cell cycle arrest, apoptosis assay and apoptosis protein expressions were determined by flow cytometry. Results: Methanolic extract of A. precatorius leaves showed the lowest IC
3.Abolition of Beauty Marks – Calamitous Burn
Saw Shier Khee ; Shawaltul Akhma Bt Harun Nor Rashid ; Norzila Ismail ; Michael Pak-Kai Wong
Malaysian Journal of Medicine and Health Sciences 2022;18(No.1):369-371
We present a case series of two young women presented with facial chemical burns after applying the mole removal
products. In this series, we describe the potentially corrosive active ingredient that produces the chemical burn in the
natural product and the off-label or unauthorised product. The burn on the delicate facial skin leads to irreversible
disfigurement. However, with timely wound debridement and a full-thickness skin graft, the reconstruction can still
achieve a satisfactory aesthetic result.
4. Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells
Husna Syakirah Ab RAHMAN ; Michael Pak-Kai WONG ; Mohd Ridzuan Abdul SAMAD ; Mohd Nizam Md HASHIM ; Wan Suriyani WAN-IBRAHIM ; Norzila ISMAIL ; Siti Farhanah MOHD-SALLEH ; Tuan Nadrah ISMAIL
Asian Pacific Journal of Tropical Medicine 2018;11(12):659-665
Objective: To identify the phytochemical compounds from Annona muricata (A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was analysed by gas chromatography mass spectrometry (GCMS) and characterized with Wiley and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7 and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of different solvent extracts of A. muricata leaves showed presence of different chemical groups of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC