1.Studies on glycosides from Gentiana veitchiorum.
Hong-Peng YANG ; Sheng QUE ; Xi-Dong WU ; Yan-Ping SHI
China Journal of Chinese Materia Medica 2008;33(21):2505-2507
OBJECTIVETo study the chemical constituents of Gentiana veitchiorum.
METHODThe chemical constituents were isolated by chromatography and identified by spectral data.
RESULTFive glycosides, loganic acid (1), gentiopicroside (2), isoorientin 3'-methyl ether (3), isovitexin (4), isoorientin (5) were isolated and identified.
CONCLUSIONCompounds 1-5 were isolated from this plant for the first time.
Apigenin ; chemistry ; Chromatography, High Pressure Liquid ; Gentiana ; chemistry ; Glucosides ; chemistry ; Glycosides ; chemistry ; Iridoid Glucosides ; Iridoids ; chemistry
2.Advances in research on chemical composition of Picrorhiza scrophulariiflora and P. kurroa and their biological activities.
Li-Zheng MA ; Li-Ping KANG ; Tie-Gui NAN ; Zhi-Lai ZHAN ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2021;46(23):6114-6129
At present, 141 compounds have been isolated from Picrorhiza scrophulariiflora and P. kurroa of the Scrophulariaceae plants, including 46 iridoid glycosides, 29 tetracyclic triterpenoids, 25 phenylpropanoids, and 11 phenylethanoid glycosides. Pharmacological studies have demonstrated that they have liver-, heart-, brain-, kidney-, and nerve cells-protecting effects as well as anti-tumor, anti-inflammatory, anti-bacterial, anti-asthma, anti-diabetic, immunomodulatory, and blood lipid-lowering activities. This article reviews the chemical components and pharmacological activities of P. scrophulariiflora and P. kurroa, aiming to provide a basis for the in-depth research, development, and utilization of the two plants.
Iridoid Glycosides
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Picrorhiza
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Triterpenes/pharmacology*
3.Simultaneous determination of five constituents in Scrophularia ningpoensis by HPLC.
Xuemei ZHANG ; Rui WANG ; Rui AN ; Ximin WU ; Xinhong WANG ; Yiming LI
China Journal of Chinese Materia Medica 2011;36(6):709-711
OBJECTIVETo develop an HPLC method for the simultaneous quantitation of five constituents in Scrophularia ningpoensis.
METHODSamples were analyzed on an Agilent SB-C18 column(4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.03% phosphate acid as mobile phases in a linear gradient mode. The flow rate was kept at 1.0 mL x min(-1), and the column temperature was set to 30 degrees C. The DAD detector wavelengths were 210, 280, 330 nm.
RESULTThe linear ranges were 50-400 mg x L(-1) for harpagide, 1-40 mg x L(-1) for harpagoside, 1-20 mg x L(-1) for cinnamic acid, 0.5-4.5 mg x L(-1) for acteoside,1-60 mg x L(-1) for angoroside C, respectively. The average recoveries of the five constituents were 100.8% (RSD 0.62%), 101.7% (RSD 0.32%), 98.8% (RSD 0.48%), 99.9% (RSD 1.4%), 99.2% (RSD 1.1%), respectively.
CONCLUSIONThrough the validation, the method was proved to be sensitive, accurate, repeatable, and can be used for quality control of the roots of S. ningpoensis.
Chromatography, High Pressure Liquid ; methods ; Cinnamates ; analysis ; Coumaric Acids ; analysis ; Glucosides ; analysis ; Glycosides ; analysis ; Iridoid Glycosides ; Phenols ; analysis ; Pyrans ; analysis ; Scrophularia ; chemistry ; Trisaccharides ; analysis
4.Qualitative and quantitative analysis on non-triterpenoids in Ligustri Lucidi Fructus.
Xin JI ; Xiao-Qian LIU ; Su-Ping XIAO ; Li-Xin YANG ; Wei-Hong FENG ; Chun LI ; Zhi-Min WANG
China Journal of Chinese Materia Medica 2019;44(8):1615-1622
In order to improve the quality control level of Ligustri Lucidi Fructus(LLF) and to explore the changes of chemical components after processing,the HPLC method for fingerprint and simultaneous determination of the major polar components in LLF were established. The octadecylsilane bonded silica gel was used as the stationary phase,with acetonitrile as the mobile phase A and0. 2% formic acid as the mobile phase B in a gradient elution procedure at a flow rate of 1. 0 m L·min-1. The detection wavelength was set at 280 nm and the column temperature was 25 ℃. There were 22 common peaks,20 of which were selected from the fingerprint of LLF and its wine-steamed product,respectively,and 14 chromatographic peaks were identified with reference substances. With the same chromatographic conditions,seven components were quantitatively analyzed and the results of system adaptability and methodology investigation all met the requirements of content determination. Compared with the crude LLF,the content of 5-hydroxymethyl furfural and salidroside significantly increased in wine-steamed LLF,while the contents of iridoid glycosides generally decreased. The method provided a basis for quality control of LLF and its processed products as well as the related preparations.
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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chemistry
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Fruit
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chemistry
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Furaldehyde
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analogs & derivatives
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Glucosides
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Iridoid Glycosides
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Ligustrum
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chemistry
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Phenols
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Phytochemicals
;
analysis
5.Isolation and characterization of phenylethanoid glycosides from Clerodendron bungei.
You-bin LI ; Jun LI ; Ping LI ; Peng-fei TU
Acta Pharmaceutica Sinica 2005;40(8):722-727
AIMTo study the chemical constituents from Clerodendron bungei Steud.
METHODSThe compounds were isolated and purified by various chromatographic techniques and identified by their physicochemical properties and spectral data.
RESULTSTen phenylethanoid glycosides were isolated and identified as clerodendronoside (1), acteoside (2), isoacteoside (3), cistanoside C (4), jionoside C (5), leucosceptoside A (6), cistanoside D (7), campneoside I (8), campneoside II (9), cistanoside F (10).
CONCLUSIONCompound 1 is a new phenylethanoid glycoside, while compounds 4-10 are obtained from this plant for the first time.
Catechols ; chemistry ; isolation & purification ; Clerodendrum ; chemistry ; Glucosides ; chemistry ; isolation & purification ; Glycosides ; chemistry ; isolation & purification ; Iridoid Glycosides ; Iridoids ; chemistry ; isolation & purification ; Molecular Structure ; Phenols ; chemistry ; isolation & purification ; Plant Components, Aerial ; chemistry ; Plants, Medicinal ; chemistry
6.Research progress on metabolism and pharmacological activities of several iridoid glycosides.
Chang-Song XUE ; Jing XU ; Cui-Ping LI ; Yu WANG ; Mei-Chen LIU ; Guang-Qing XIA
China Journal of Chinese Materia Medica 2018;43(1):39-45
As a large category of natural products widely present in traditional Chinese medicine, iridoid glycosides have multiple pharmacological activities. Recent researches suggest that iridoid glycosides mainly exist in the forms of original form, aglycone and a series of their Ⅰ and Ⅱ metabolites under the biotransformation effect, and their metabolites have been proved to have multiple pharmacological activities. The research progress on metabolism and metabolite activities of several iridoid glycosides would be reviewed in this article, to provide a theoretical basis for the further development and utilization of iridoid compounds and their metabolites.
Drugs, Chinese Herbal
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metabolism
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pharmacology
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Humans
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Iridoid Glycosides
;
metabolism
;
pharmacology
7.Research progress on chemical constituents and pharmacological activities of iridoid glycosides in Lonicera japonica.
Ran YANG ; Yuan LU ; Hao HAO ; Man-da ZHANG ; Jing XUAN ; Yong-Qing ZHANG
China Journal of Chinese Materia Medica 2021;46(11):2746-2752
As a traditional Chinese medicinal material, Lonicera japonica has a long medicinal history. The chemical constituents of Lonicera japonica are complex, mainly including iridoid glycosides, flavonoids, triterpenes, organic acids and volatile oil. Iridoid glycosides account for a higher proportion. In addition, modern pharmacological studies have shown that the iridoid glycosides have many pharmacological activities such as antivirus, anti-inflammation, anti-tumor, liver protection and lowering blood sugar. This review intends to systematically summarize the iridoid glycosides identified from Lonicera japonica and their pharmacological activities by searc-hing Chinese and English databases, in order to provide a reference for the further development and utilization of Lonicera japonica and for the improvement of quality standards of medicinal materials.
Anti-Inflammatory Agents
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Flavonoids
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Glycosides/pharmacology*
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Iridoid Glycosides/pharmacology*
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Lonicera
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Plant Extracts
8.HPLC fingerprinting of total glycosides of Swertia franchetiana.
Wei TIAN ; Zhao-hui CHEN ; Jing ZHAI ; Li-ren CHEN ; Yong-min LI
Acta Pharmaceutica Sinica 2005;40(5):447-452
AIMTo establish a sensitive and specific HPLC method for controlling the quality of total glycosides from Swertia franchetiana H. Smith.
METHODSHPLC method was applied for quality and quantitative assessment of the pharmaceutical extracts from Swertia franchetiana H. Smith. The preparation of sample, the HPLC column, mobile phase, elution mode (isocratic or gradient) and gradient program were optimized in order to obtain HPLC profile. The HPLC system consisted of a SPD-1OAvp pump, SPD-M1OAVP photodiode-array detector (PAD), SIL-10ADVP auto injector. Data were acquired and processed with the CLASS-VP6.1 workstation. HPLC analysis was performed on a Kromasil C18 column (250 mm x 4. 6 mm ID, 5 microm) with methanol and water as mobile phase. The column temperature was set up at 40 degrees C and the flow-rate was 1 mL x min(-1). The reference solution of chemical standards and sample were injected into HPLC system, separately.
RESULTSThe HPLC chromatographic fingerprinting of the total glycosides, showing 16 characteristic peaks which were partitioned into three parts: one peak in 0-10 min of retention time, nine peaks containing main 1-7 peaks in 10-15 min of retention time, 6 peaks in 15-30 min of retention time, was established from 10 lots of their products. By comparison of the retention time and the on-line UV spectra and their molecule weights of chemical standards, peak 1-7 were identified as swertiamarin (1), gentiopicroside (2), sweroside (3), isoorientin (4), swertisin (5), isoswertisin (6) and swetianolin (7), respectively. The ratios of peak area between 1-16 were in their extent. Moreover, comparison of the HPLC profiles of the total glycosides, the extracts prepared using another process and the plant indicated that they were closely related to each other.
CONCLUSIONThe HPLC profiles and quantitative assessment of the total glycosides from Swertia franchetiana H. Smith with high specificity can be used to control their quality and assure lot to lot consistency.
Chromatography, High Pressure Liquid ; methods ; Glucosides ; analysis ; chemistry ; Glycosides ; analysis ; chemistry ; Iridoid Glucosides ; Iridoids ; analysis ; chemistry ; Luteolin ; analysis ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry ; Pyrans ; analysis ; chemistry ; Pyrones ; analysis ; chemistry ; Quality Control ; Swertia ; chemistry
9.Determination of three glycosides from herbs of Swertia punicea by RP-HPLC.
Xiu-qiao ZHANG ; Feng-jiao HUANG ; Jiao-chun CHEN ; Yan-wen LIU
China Journal of Chinese Materia Medica 2007;32(12):1184-1186
OBJECTIVETo develop a RP-HPLC method for determination of three glycosides in Swertia punicea.
METHODChromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C.
RESULTThe calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5).
CONCLUSIONIt is a simple and sensitive meathod in controlling the quality of S. punicea.
Calibration ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; standards ; Glucosides ; analysis ; Glycosides ; analysis ; Iridoid Glucosides ; Iridoids ; analysis ; Plants, Medicinal ; chemistry ; Quality Control ; Reproducibility of Results ; Swertia ; chemistry ; Xanthones ; analysis
10.Promoting effect of constituents in plasma after oral administration of liuwei dihuangwan on proliferation of rat osteoblast.
Hui SUN ; Ning ZHANG ; Li-jing LI ; Xi-jun WANG
China Journal of Chinese Materia Medica 2008;33(17):2161-2164
OBJECTIVETo elucidate the promoting effect of the constituents in plasma after oral administration of Liuwei Dihuangwan on proliferation of rat osteoblast.
METHODTo add the constituents in plasma after oral administration of Liuwei Dihuangwan with rational concentration into culture solution of rat osteoblast, later using MT method for determining the proliferation rate of rat osteoblast.
RESULTThe Mixed group including morroniside, sweroside and loganin with different dose all significantly promoted proliferation of rat osteoblast.
CONCLUSIONThis study preliminarily elucidated the constituents such as morroniside, sweroside and loganin in plasma after oral administration of Liuwei Dihuangwan were main biological constituents which contributed to anti-osteoporosis bioactivity of Liuwei Dihuangwan.
Administration, Oral ; Animals ; Cell Proliferation ; drug effects ; Cells, Cultured ; Drugs, Chinese Herbal ; administration & dosage ; Glucosides ; blood ; Glycosides ; blood ; Iridoid Glucosides ; Iridoids ; blood ; Osteoblasts ; cytology ; drug effects ; Plasma ; chemistry ; drug effects ; Rats ; Rats, Wistar