1.Fluoroscopic Characterization of Colonic Dysmotility Associated to Opioid and Cannabinoid Agonists in Conscious Rats
Susana DÍAZ-RUANO ; Ana E LÓPEZ-PÉREZ ; Rocío GIRÓN ; Irene PÉREZ-GARCÍA ; María I MARTÍN-FONTELLES ; Raquel ABALO
Journal of Neurogastroenterology and Motility 2019;25(2):300-315
BACKGROUND/AIMS: Gastrointestinal adverse effects have a major impact on health and quality of life in analgesics users. Non-invasive methods to study gastrointestinal motility are of high interest. Fluoroscopy has been previously used to study gastrointestinal motility in small experimental animals, but they were generally anesthetized and anesthesia itself may alter motility. In this study, our aim is to determine, in conscious rats, the effect of increasing doses of 2 opioid (morphine and loperamide) and 1 cannabinoid (WIN 55,212-2) agonists on colonic motility using fluoroscopic recordings and spatio-temporal maps. METHODS: Male Wistar rats received barium sulfate intragastrically, 20–22 hours before fluoroscopy, so that stained fecal pellets could be seen at the time of recording. Animals received an intraperitoneal administration of morphine, loperamide, or WIN 55,212-2 (at 0.1, 1, 5, or 10 mg/kg) or their corresponding vehicles (saline, Cremophor, and Tocrisolve, respectively), 30 minutes before fluoroscopy. Rats were conscious and placed within movement-restrainers for the length of fluoroscopic recordings (120 seconds). Spatio-temporal maps were built, and different parameters were analyzed from the fluoroscopic recordings in a blinded fashion to evaluate colonic propulsion of endogenous fecal pellets. RESULTS: The analgesic drugs inhibited propulsion of endogenous fecal pellets in a dose-dependent manner. CONCLUSIONS: Fluoroscopy allows studying colonic propulsion of endogenous fecal pellets in conscious rats. Our method may be applied to the noninvasive study of the effect of different drug treatments and pathologies.
Analgesics
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Anesthesia
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Animals
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Barium Sulfate
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Cannabinoid Receptor Agonists
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Cannabinoids
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Colon
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Fluoroscopy
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Gastrointestinal Motility
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Humans
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Loperamide
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Male
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Methods
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Morphine
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Pathology
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Quality of Life
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Rats
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Rats, Wistar
2.Clinical Outcomes of Mechanical Thrombectomy in Stroke Tandem Lesions According to Intracranial Occlusion Location
Elena ZAPATA-ARRIAZA ; Asier de ALBÓNIGA-CHINDURZA ; Joaquin ORTEGA-QUINTANILLA ; Irene ESCUDERO-MARTÍNEZ ; Francisco MONICHE ; Manuel MEDINA-RODRÍGUEZ ; Blanca PARDO-GALIANA ; Juan Antonio Cabezas RODRÍGUEZ ; Lucía Lebrato HERNÁNDEZ ; Leire AINZ ; Soledad PÉREZ-SÁNCHEZ ; Ana DOMÍNGUEZ-MAYORAL ; Ana BARRAGÁN ; Aurelio CAYUELA ; Joan MONTANER ; Alejandro González GARCÍA
Journal of Stroke 2021;23(1):124-127
3.Sex Differences by Hospital-Level in Performance and Outcomes of Endovascular Treatment for Acute Ischemic Stroke
Soledad PÉREZ-SÁNCHEZ ; Ana BARRAGÁN-PRIETO ; Joaquín ORTEGA-QUINTANILLA ; Ana DOMÍNGUEZ-MAYORAL ; Miguel Ángel GAMERO-GARCÍA ; Elena ZAPATA-ARRIAZA ; Reyes de TORRES-CHACÓN ; Asier de ALBÓNIGA-CHINDURZA ; Montserrat ZAPATA-HIDALGO ; Francisco MONICHE ; Irene ESCUDERO-MARTÍNEZ ; Pablo BAENA ; Juan Antonio CABEZAS ; Juan Miguel OROPESA-RUIZ ; Gema SANZ-FERNÁNDEZ ; Alejandro GONZÁLEZ ; Joan MONTANER
Journal of Stroke 2020;22(2):258-261