1.CDX1 and CDX2 Expression in Intestinal Metaplasia, Dysplasia and Gastric Cancer.
Jung Mook KANG ; Byoung Hwan LEE ; Nayoung KIM ; Hye Seung LEE ; Hee Eun LEE ; Ji Hyun PARK ; Joo Sung KIM ; Hyun Chae JUNG ; In Sung SONG
Journal of Korean Medical Science 2011;26(5):647-653
Intestinal metaplasia (IM) has been regarded as a premalignant condition. However, the pathogenesis of IM is not fully understood. The aim of this study was to evaluate the role of CDX1 and CDX2 in the formation of IM and the progression to dysplasia and gastric cancer (GC). A total of 270 subjects included 90 with GC, dysplasia and age- and sex-matched controls. Real-time PCR (RT-PCR) was performed with body specimens for CDX1 and CDX2. The expression of CDX2 was significantly higher in H. pylori positive group than H. pylori negative group (P = 0.045). CDX1 and CDX2 expression increased proportional to the IM grade of the body (P < 0.001). CDX2 expression was significantly higher in incomplete type of IM than in complete type (P = 0.045). The expression of CDX1 in dysplasia group was significantly higher than in the control group (P = 0.001); in addition, CDX1 and CDX2 in cancer group was significantly higher than control group (P < 0.001, and P < 0.001, respectively). Aberrant expression of CDX1 and CDX2 correlated with H. pylori infection and grade of IM in the body. Furthermore, the results suggest that CDX1 and CDX2 play a role in the progression to GC and dysplasia.
Aged
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Female
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Helicobacter Infections/complications/microbiology
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Helicobacter pylori/isolation & purification
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Homeodomain Proteins/*genetics/metabolism
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Humans
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Intestinal Diseases/*genetics/microbiology/pathology
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Male
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Metaplasia/pathology
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Middle Aged
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Polymerase Chain Reaction
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Precancerous Conditions/metabolism/pathology
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Stomach Neoplasms/etiology/*genetics/microbiology
2.Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR.
Dong Kyun SUH ; Suk Kyung LYM ; You Chan BAE ; Keun Woo LEE ; Won Pil CHOI ; Jae Chan SONG
Journal of Veterinary Science 2000;1(1):33-37
Lawsonia intracellularis is not culturable with a standard bacteriologic culture. One step PCR assay as a clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis (PPE) caused by L. intracellularis. Primers were designed based on the p78 DNA clone of L. intracellularis. The one step PCR resulted in the formation of a specific 210-bp DNA product derived from L. intracellularis. The nonspecific amplification product was not detected with swine genomic DNA or other bacterial strains causing similar symptoms to L. intracellularis infection. The one step PCR was as sensitive as 100 pg of L. intracellularis genomic DNA. We applied this method to field specimens diagnosed as PPE by macroscopic observation. Of 17 mucosal scraping specimens, 16(94%) were identified as positive to PPE and 15(88%) of 17 feces specimens. These results suggest that the one step PCR can be used as a rapid diagnostic method for L. intracellularis infection.
Animals
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Base Sequence
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DNA Primers
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Desulfovibrionaceae Infections/diagnosis/*veterinary
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Ileum/microbiology/pathology
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Intestinal Mucosa/microbiology/pathology
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Lawsonia Bacteria/genetics/*isolation & purification
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Polymerase Chain Reaction/*methods
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Reproducibility of Results
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Sensitivity and Specificity
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Swine
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Swine Diseases/*diagnosis/microbiology