Mucosal mast cell-derived chondroitin sulphates (sulphated proteoglycans) were assayed in gut washings and homogenate of FcRgamma-knockout (KO) and wild-type (WT) C57BL/6 mice challenged with Strongyloides venezuelensis in order to assess their possible role in secondary immunity against enteric nematodes. Groups of immune KO and WT mice were challenged by oral gavage with 300 infective larvae (L3). Establishment of infection was assessed by daily faecal analysis to determine the number of eggs per gram of faeces (EPG) and by adult worm recovery on days 5 and 13 post challenge. Mucosal mast cell (MMC) counts were done on days 5 and 13 post challenge while MMC-derived chondroitin sulphates in gut washings (days 1 and 5) and homogenate (day 8) were assayed by high performance liquid chromatography (HPLC). Results showed that patent infection occurred in challenged KO but not WT mice despite significantly higher mastocytosis in jejunal sections of KO than WT mice (p<0.001). Similarly but against prediction, significantly higher concentration of MMC-derived chondroitin sulphates was observed in gut homogenate of KO than WT mice (p<0.05). In contrast, significantly higher concentration of chondroitin sulphates was observed in gut washings of WT than KO mice (p<0.05). These results suggest that MMC in KO mice failed to release sufficient amount of sulphated proteoglycans into the gut lumen as did the WT mice, which may have been part of the hostile environment that prevented the establishment in and eventual expulsion of adult S. venezuelensis from the gut of WT mice following challenge.
Animals ; Cell Count/veterinary ; Chondroitin Sulfates/*immunology/metabolism ; Chymases ; Feces/parasitology ; Intestinal Diseases, Parasitic/immunology/*veterinary ; Intestinal Mucosa/cytology/immunology/parasitology ; Jejunum/cytology/immunology/parasitology ; Male ; Mast Cells/immunology/metabolism/*parasitology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Parasite Egg Count/veterinary ; Receptors, IgG/*immunology ; Serine Endopeptidases/blood/immunology ; Specific Pathogen-Free Organisms ; Strongyloides/*immunology ; Strongyloidiasis/immunology/parasitology/*veterinary