Intestinal Absorption ; Schistosomicides/*metabolism ; Solubility

OBJECTIVES: We compared the effects of two different nano-sized calcium supplements (synthesized by wet-chemical method or the dry-grinding method of calcium carbonate) and one micro-sized calcium supplement (calcium carbonate) on intestinal absorption and bone turnover in 20 young women. MATERIALS & METHODS: This study was carried out with a three week cross-over design. The subjects ingested one of three different calcium supplements (1 dose of 500 mg Ca++) at 8 AM, which was repeated three times weekly. Serum and urine samples were collected before and after the calcium load using a time table (serum sampling 0, 2 hr, 4 hr, 8 hr after loading; urine sampling 0, 4 hr, 8 hr after loading). RESULTS: Serum ionized calcium and parathyroid hormone concentrations significantly changed in response to all calcium supplements loading, the difference among calcium supplements was not significant. However, the postload urinary excretion of calcium and the N-telopeptide/creatinine ratio were significantly different among three calcium supplement over time by repeated measured ANOVA. CONCLUSIONS: The results of this study suggest the effect of nano-sized calcium supplements synthesized by the wet-chemical method is superior to the other calcium supplements in intestinal calcium absorption and bone turn-over.
Absorption ; Calcium ; Cross-Over Studies ; Intestinal Absorption ; Parathyroid Hormone

Animals ; Intestinal Absorption ; Mice ; Schistosomicides ; metabolism ; Solubility

To study the effect of self-assembled nanoparticles from Shaoyao Gancao Decoction(SGD-SAN) on the encapsulation, in vitro release and intestinal absorption of the main components of Baishao. Particle size analysis and morphological observation were used to verify the formation of SGD-SAN in the decoction. The entrapment efficiency(EE) of SGD-SAN on the main components of Baishao was determined by ultrafiltration centrifugation. The dialysis bag method was used to study the in vitro release of the main components of Baishao with pH 6.8 phosphate buffer solution as the release media. Single-pass intestinal perfusion study was performed to investigate the effect of SGD-SAN on the absorption of the main components of Baishao. The results showed that there were nanoparticles in the SGD, and the particle sizes and PDI of SGD-SAN were about 200 nm and 0.38, respectively. SGD-SAN was irregularly spherical under transmission electron microscope(TEM). The EEs of albiflorin, paeoniflorin and benzoylpaeoniflorin in SGD-SAN were 33.78%±1.03%,33.61%±0.90%,88.53%±0.58%, respectively. The release characteristics of albiflorin, paeoniflorin and benzoylpaeoniflorin from SGD-SAN showed a slow-release effect on pH 6.8 phosphate buffer solution media. SGD-SAN could significantly enhance the absorption of albiflorin, paeoniflorin and benzoylpaeoniflorin in the ileum. The results of this study indicated that SAN could be formed during the mixed decoction of Baishao and Gancao, and SGD-SAN could encapsulate the components of Baishao, with a certain slow-release effect, and the formation of SAN facilitated the absorption of drugs in the ileum.
Drugs, Chinese Herbal ; Intestinal Absorption ; Intestines ; Nanoparticles

When about 300mg of L-ascorbic acid was given intravenously to the adults saturated with the vitamin, the increased blood level returned to almost the original level in 3 hours. Also the amounts of the vitamin absorbed by the tissue cells and metabolized in the body or excreted into the gastrointestinal tract were found to average 74.1mg in 3 hours. When about 300mg of L-ascorbic acid was given intravenously twice at intervals of 3 hours, an average of 101 mg of the vitamin was absorbed by the tissue cells and metabolized in the body or excreted into the gastrointestinal tract in 6 hours. When the vitamin was given orally to the saturated adults, the absorption rate increased as the test does increased. When large amounts (3.0gm) of the vitamin were given, the absorption rate reached the maximum. The maximum absorption in 6 hours was estimated as 681 mg of the vitamin.
Adult ; Ascorbic Acid/blood/*metabolism ; Human ; *Intestinal Absorption

For the purpose of estimating the maximum absorption of riboflavin and thiamine from the intestine, large test doses of riboflavin and thiamine were administered orally to apparently normal male subjects, receiving normal Korean diets, and their maximum absorption were estimated by determining the excretion in urine. The maximum absorption of riboflavin and thiamine were estimated 10.700 and 3.800 mg. respectively.
Human ; Intestinal Absorption* ; Male ; Riboflavin/metabolism* ; Thiamine/metabolism*

OBJECTIVETo establish a method for quick finding of the absorption ingredients of Paeoniae Radix Alba in order to select the index of quality control.

METHODThe absorption ingredients of three concentration of Paeoniae Radix Alba were investigated with the in vitro-everted intestinal sac (VEIS) model. The intestinal sac fluids of jejunum and ileum were collected in different time and detected by HPLC. The accumulative absorption quantity of albiflorin and paeoniflorin were calculated, respectively.

RESULTFive ingredients could be detected. In different concentrations of Paeoniae Radix Alba, albiflorin and paeoniflorin in various intestinal sections were the linear absorption (R2 > 0.9), conformed to the zero order absorption rate. The values of Ka in the jejunum and ileum were increased along with the raised dosage of the Paeoniae Radix Alba (P < 0.05), indicating a passive absorption manner.

CONCLUSIONSEMAC could be used as a tool to find the absorption ingredients of Paeoniae Radix Alba. Compared with the jejunum, the ileum could provide the more absorption information. It was showed that the optimal detecting time was 60 min.

To establish an in situ single-way intestinal perfusion model, in order to study the intestinal absorption kinetics of AP. The concentration of AP in the perfusate was determined by HLPC. The results showed different AP concentrations in all intestinal segments, with the fastest absorption rate in duodenum, which was followed by jejunum, ileum and colon. In general, the constant absorption rate (Ka) of AP in duodenum and jejunum first increased and then decreased with the rise in drug concentration (P <0. 05); the absorption mechanism may be related to active transport and facilitated diffusion factors. The constant absorption rate (Ka) of AP in ileum and colon generally kept unchanged with the rise diffusion in drug concentration, the absorption mechanism may be related to passive.
Animals ; Apigenin ; metabolism ; Intestinal Absorption ; physiology ; Male ; Rats

The rat everted intestinal sac model was adopted to investigate the absorption of total flavonoids from Coreopsis tinctoria in different intestinal segments. Cyaniding-3-O-β-D-glucoside, chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, iso-okanin, marein and 3,5-dicaffeoylquinic acid which as the major chemical components of total flavonoids from C. tinctoria were selec-ted as the study objects to evaluate the absorption characteristics of each component in different intestinal segments. The results showed that the absorption of seven components of total flavonoids at different intestinal segments was in consistent with zero order absorption rate. The K_a of chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, isookanin and 3,5-dicaffeoylquinic acid increased with increasing of concentration of total flavonoids(P<0.05), indicating that the intestinal absorption of these five components was passive transport. The K_a of cyaniding-3-O-β-D-glucoside and marein showed a weak concentration dependence, suggesting that the absorption of them may be an positive and passive co-existing mode. The result of absorption in different intestinal segments showed that cyaniding-3-O-β-D-glucoside, chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, marein and 3,5-dicaffeoylquinic acid were mainly absorbed in ileum, while isookanin was mainly absorbed in jejunum. The total flavonoids of C. tinctoria are selectively absorbed in intestinal tract, the rat everted intestinal sac model can be used to evaluate the multi-component intestinal absorption characteristics of total flavonoids from C. tinctoria.
Animals ; Chlorogenic Acid ; Coreopsis ; Flavonoids ; Intestinal Absorption ; Plant Extracts ; Rats

Intestinal absorption liquid was prepared by using everted intestinal sac method; meanwhile, its recipes were decomposed or restructured. Platelet aggregation activity was examined by biochemical tests and a microplate reader. One or more kinds of Chinese medicines which displayed inhibiting activity in Naoxintong Capsules were screened through separation and combination of prescription. The results showed that Naoxintong Capsules could inhibit ADP-induced platelet aggregation. Recipe decomposition and restructuring results showed that Salviae Miltiorrhizae Radix et Rhizoma, Paeoniae Radix Rubra, Cinnamomi Ramulus and Hirudo were the main effective medicines in inhibiting platelet aggregation. Furthermore, Cinnamomi Ramulus played a vital role in inhibiting activity among those four kinds of Chinese medicines. Coumarin derived from intestinal absorption liquid of Cinnamomi Ramulus had inhibiting activity in the range of 50-200 μmol·L⁻¹, and other ingredients such as cinnamyl alcohol and cinnamaldehyde also had inhibiting activities. In conclusion, Salviae Miltiorrhizae Radix et Rhizoma, Paeoniae Radix Rubra, Cinnamomi Ramulus and Hirudo are the main components for inhibiting ADP-induced platelet aggregation, and Cinnamomi Ramulus has the most strongest inhibiting activity in Naoxintong Capsules.
Adenosine Diphosphate ; Capsules ; Drugs, Chinese Herbal ; Intestinal Absorption ; Platelet Aggregation