2.Is This the Era of Interstitial Cells of Cajal Transplantation?.
Journal of Neurogastroenterology and Motility 2014;20(3):281-283
No abstract available.
Interstitial Cells of Cajal*
3.Voltage-dependent Ca2+ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach.
Young Chul KIM ; Hikaru SUZUKI ; Wen Xie XU ; Hikaru HASHITANI ; Woong CHOI ; Hyo Yung YUN ; Seon Mee PARK ; Sei Jin YOUN ; Sang Jeon LEE ; Sang Jin LEE
The Korean Journal of Physiology and Pharmacology 2008;12(6):323-330
The properties of voltage dependent Ca2+ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min(-1), respectively, in the presence of 1micrometer nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K+-rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs+-rich pipette solution at Vh=?80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1micrometer) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca2+ currents (VDCCL).
Interstitial Cells of Cajal
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Nifedipine
;
Stomach
5.Overexpression of Stromal Interaction Molecule 1/Store-operated Calcium Entry-associated Regulatory Factor in Interstitial Cells of Cajal in Mouse Jejunum Impairs Pacemaker Activity
LV JIAN-LIN ; Ning Yuan CHEN ; Shang Ling PAN
Journal of Neurogastroenterology and Motility 2018;24(4):678-680
No abstract available.
Animals
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Calcium
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Interstitial Cells of Cajal
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Jejunum
;
Mice
6.Aging Decreases the Density of Colonic Interstitial Cells of Cajal Associated With Constipation in Rats
Journal of Neurogastroenterology and Motility 2018;24(2):326-328
No abstract available.
Aging
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Animals
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Colon
;
Constipation
;
Interstitial Cells of Cajal
;
Rats
7.Electrophysiological Characteristics of Human Colon Circular Muscle.
Eun Kyung CHOE ; Seung Bum RYOO ; Sang Hui MOON ; Jung Sun MOON ; Kyu Joo PARK
Journal of the Korean Society of Coloproctology 2010;26(2):105-110
PURPOSE: Interstitial cells of Cajal (ICC) play a core function in colonic motility as a pacemaker by regulating the slow wave. We intended to investigate the electrophysiological characteristics of the circular smooth muscle in the human colon, especially in Koreans. METHODS: Colon tissues were obtained from patients undergoing an elective colectomy for non-obstructive lesions. Tissues were immediately stored in oxygenated Krebs-Ringer's bicarbonate solution, and conventional microelectrode recordings of the colonic circular muscle were done from muscle cells. RESULTS: The trans-membrane potentials from 100 tissues were recorded. Regular slow-wave patterns were observed in 36 patients, and 64 patients showed irregular wave patterns. In the tissues exhibiting regular slow-wave patterns, 20 tissues were recorded from the proximal colon and 16 from the distal colon. The frequency of the slow wave from the proximal part was significantly higher than that from the distal part. The resting membrane potential, the upstroke amplitude, the spike amplitude, and the maximal rate of rise showed no significant difference between the two parts, but the time to reach half amplitude did show a significant difference between the two parts. No significant differences in parameters were observed based on the patients' demographics such as sex and age (younger or older than 60). We were able to divide the irregular waves into 3 groups. Irregular slow waves were observed in 35 tissues, irregular slow waves mixed with spike waves were observed in 26 tissues, and spike waves alone were observed in 3 tissues. CONCLUSION: We analyzed the electrophysiological characteristics of the human colonic circular smooth muscle, especially those of the slow wave originating from ICC of patients who had not been pretreated with drugs.
Colectomy
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Colon
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Demography
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Humans
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Interstitial Cells of Cajal
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Membrane Potentials
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Microelectrodes
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Muscle Cells
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Muscle, Smooth
;
Muscles
;
Oxygen
8.Repetitive Colonoscopic Decompression as a Bridge Therapy before Surgery in a Pregnant Patient with Chronic Intestinal Pseudo-Obstruction.
Joon Sung KIM ; Bo In LEE ; Byung Wook KIM ; Hwang CHOI ; Yun Seok LEE ; Leeso MAENG
Clinical Endoscopy 2013;46(5):591-594
Chronic intestinal pseudo-obstruction is a rare clinical syndrome which is characterized by intestinal obstruction without occluding lesions in the intestinal lumen and pregnancy is one of the important aggravating factors. Here, we report a case of a woman with intractable intestinal pseudo-obstruction that was precipitated by pregnancy. She could not make any stool passage for more than 4 weeks until a fetal gestational age of 17 weeks was reached. However, the patient could be maintained by repetitive colonoscopic decompressions and finally total colectomy could be performed successfully at a fetal gestational age of 21 weeks.
Colectomy
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Decompression
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Female
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Gestational Age
;
Humans
;
Interstitial Cells of Cajal
;
Intestinal Obstruction
;
Intestinal Pseudo-Obstruction
;
Pregnancy
9.A Case of Chronic Neuropathic Pseudo-obstruction of the Colon.
Sunyong KIM ; Min Kyung KIM ; Uk JO ; Jung Min CHAE ; Jae Myung CHA ; A Ri SHIN ; Sung Jig LIM ; Ahrim MOON
Korean Journal of Gastrointestinal Endoscopy 2011;42(3):179-184
Colonic pseudo-obstruction, which is characterized by repetitive episodes or continuous symptoms and signs of a bowel obstruction, is a rare disease that results in colorectal dilatation without any obstructing lesions. Cases with neuropathic chronic colonic pseudo-obstruction have rarely been reported in Korea. We report a case of neuropathic colonic pseudo-obstruction, which results in decreased number of interstitial cells of Cajal and hypoganglionosis, in a 50-year-old man with chronic constipation accompanied by megacolon.
Colon
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Colonic Pseudo-Obstruction
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Constipation
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Dilatation
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Humans
;
Interstitial Cells of Cajal
;
Korea
;
Megacolon
;
Middle Aged
;
Rare Diseases
10.Experimental and Automated Analysis Techniques for High-resolution Electrical Mapping of Small Intestine Slow Wave Activity.
Timothy R ANGELI ; Gregory O'GRADY ; Niranchan PASKARANANDAVADIVEL ; Jonathan C ERICKSON ; Peng DU ; Andrew J PULLAN ; Ian P BISSETT ; Leo K CHENG
Journal of Neurogastroenterology and Motility 2013;19(2):179-191
BACKGROUND/AIMS: Small intestine motility is governed by an electrical slow wave activity, and abnormal slow wave events have been associated with intestinal dysmotility. High-resolution (HR) techniques are necessary to analyze slow wave propagation, but progress has been limited by few available electrode options and laborious manual analysis. This study presents novel methods for in vivo HR mapping of small intestine slow wave activity. METHODS: Recordings were obtained from along the porcine small intestine using flexible printed circuit board arrays (256 electrodes; 4 mm spacing). Filtering options were compared, and analysis was automated through adaptations of the falling-edge variable-threshold (FEVT) algorithm and graphical visualization tools. RESULTS: A Savitzky-Golay filter was chosen with polynomial-order 9 and window size 1.7 seconds, which maintained 94% of slow wave amplitude, 57% of gradient and achieved a noise correction ratio of 0.083. Optimized FEVT parameters achieved 87% sensitivity and 90% positive-predictive value. Automated activation mapping and animation successfully revealed slow wave propagation patterns, and frequency, velocity, and amplitude were calculated and compared at 5 locations along the intestine (16.4 +/- 0.3 cpm, 13.4 +/- 1.7 mm/sec, and 43 +/- 6 microV, respectively, in the proximal jejunum). CONCLUSIONS: The methods developed and validated here will greatly assist small intestine HR mapping, and will enable experimental and translational work to evaluate small intestine motility in health and disease.
Electrodes
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Electrophysiology
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Forced Expiratory Volume
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Gastrointestinal Motility
;
Interstitial Cells of Cajal
;
Intestine, Small
;
Intestines
;
Noise