Objective To find a proper way for accurate results of blood counts,and provide reference for the user of 3 cluster blood cell analyzer.Methods Based on the results of blood cell analyzer KX21 and International Consensus Review Rules,set up the criteria for blood cell microscopic examination,1700 blood specimen were detected and the results were analyzed according to the criteria..Statistics analysis were made to evaluate the accordance between warnings of analyzer and manual examination,likewise the reliability of the criteria.Results According to the review rules and slide riview positive criteria[1],the true positive ratio,the false positive ratio,the true negative ratio and the false negative ratio were 11.88%(202/1 700),21.88%(372/1 700),64.29% (1 093/1 700) and 1.94% (33/1 700) respectively..No blast cell and immature cell was missed.Conclusion The smear review criteria suggested by the International Consensus Group could facilitate to establish the review criteria for 3 cluster blood cell analyzer..The review rules used on KX21 blood cell analyzer in this study are suitable for our laboratory.

Objective To identify a case of Para-Bombay phenotype and analyze it's molecular background.Methods Routine serological methods were used to identify the antigens on red cells and antibodies in serum,the weak antigens on red cells were detected by absorption and elution test.ABO substances were determined by agglutination inhibition test in saliva.FUT1 and FUT2 gene were sequenced with molecular methods.Results The para-Bombay was confirmed as Ah-secretion.Two of the three T repeats located at the nucleotides 880-882 were deleted.This deletion causes the shifting of the open-reading frame.The FUT2 genotype was Se357Se357.Conclusion Para-Bombay phenotype is rare.Homozygous genotype of FUT1 allele,hh(nt880-882de1TT)might be the cause of H-antigens deficiency..The wild type of FUT2 allele was consistent with the secretor status.

Objective To know the etiology distribution and drug resistance in neonate, hope to provide the reference for clinic.Methods The identification and susceptibility of bacteria were detected by culture.Results 583 bacterial strains were identified from total 560 positive samples from 2127 neonate (25.8%).The infection neonates of single bacterial and complex bacterial were 537 and 23 respectively of all 560 patients.The ratio of staphylococcus aureus, klebsiella pneumoniae and E.coli were 29.5%,22.0% and 17.0% respectively in total 560 patients.Staphylococcus Aureus of Resistance to Vancomycin(VRSA) wasn't be found.Conclusion Respiratory tract infection is the major cause of infection in newborn, including staphylococcus aureus, klebsiella pneumoniae and E.coli.

Objective To investigate the distribution and the characteristics of HBV genotype in Kunming City,China.Methods The HBV genotypes and HBV-DNA levels of 360 patients in Kunming were detected by real-time quantitative PCR using type-specific primers,the serum-types were also detected in ELISA at the same time.Results Of the 360 specimens,346(96.11%) could be detected directly,among them 196 were genotype C(56.65%),91 were genotype B(26.30%),91 were genotype B(26.30%),37 were in B-C mix-type (10.69%),21 were genotype D(6.07%),1 were genotype A(0.29%).Among them,the genotype C was higer than the others (P<0.01);HBV-DNA levels of the patients with genotype C and B-C mix-type were higer than the genotype B and the others(P<0.01,P<0.05) (beside the positive proportions of genotype B was lower in the serum with"HB-sAg( + ),HBeAg( + ),anti-HBc( + )"(P<0.01),the other genotypes were no relation with their serum-types (P>0.05);the 360 specimens,14(3.89%)could not be detected directly,the average level of them was 4.136X 103IU/ml,it was lower than that of others(P<0.05).Conclusion The HBV-genotypes in Kunming area are abundant,including genotype C,B,D,A and B/C mix-type.The dominant genotype of HBV are C and B,and the HBV-DNA level of genotypes B/C mix-type and C were singnificantly higher than that of others ;The negative relation existed between the genotype B's frequency and the positive proportions of HBeAg;The genotype D frequency of this region is higher than that of lower-altitude inland areas and lower than that of plateau areas around Lahsa,which probably stands for its epidemiologic feature.Meanwhile,those samples without direct identification contain relatively lower level of HBV-DNA,which probably suggests that HBV genotype should be conducted on a basis of a certain HBV-DNA quantity.

Objective To investigate the Clinical Value of Combined Determination of Serum cystatin C and Retinol-binding protein in diabetic nephropathy.Methods Immunoturbidimetry was employed to analyze Cystatin C(Cys C)and Retinol-binding protein;(RBP) from 94 cases of diabetic patients and 110 healthy control group .and we also detected thevalue of serum creatinine (Scr),urinary creatinine (Ucr),and calculated creatinine clearance rate (Ccr),compared the correlation of Cys C,RBP with Ccr,Scr.Results The contents of serum Cys C,RBP in the group of diabetic nephropathy were significant higher than that in healthy controllers(P<0.01),and Cys C,RBP were significantly negative correlation with Ccr.Conclusion The sensitivity and accuracy of the combined determination of serum Cys C,RBP in diabetic nephropathy are improved and might provide valuable proof for early diagnostic in diabetic nephropathy.

Objective To evaluate the applicability of the international criteria for blood smear review on the SysmexXE-2100 hematology analyzer.Methods 300 blood samples were tested by hematology analyzer Sysmex-XE2100,then it were divided into two groups,that is,sieving group and sieved group according to the criteria of international boold smear review,then blood smears were reviewed with optical microscope.Results Among 300 samples,,54 cases were true positive(18%),true negative was 64%,48 cases were false positive(16%),four 6cases were false-negative (2%),the accuracy rate was 82%.Conclusion Performance of microscopic observation after analyzer detection according to the international criteria for blood smear review presented good efficiency,it will show better practical value analysising with the clinical condition.

Objective To observe the value of adenosine deaminase(ADA)in differential diagnosis of hydrothorax.Methods In this study,93 subjects were collected in the second Xiangya hospital from Oct 2007 to Mar 2008.The patients were divided into three groups.which were the malignant hydrothorax group,the tuberculous hydrothorax group and the transudative hydrothorax group.We measured the adenosine deaminase(ADA)in pleural effusion of every patients with continuous monitored enzymatic method.Results The values of ADA in the three group were(14.24±12.90)U/L,(49.36±15.75)U/L and(10.12±7.87)U/L,respectively.There were significant differences for the values of adenosine deaminase(ADA)among the three groups,For instance,The value of adenosine deaminase(ADA)in the tuberculous hydrothorax group was greatly different with that of the transudative hydrothorax group(P＜0.01).Conclusion Detection of adenosine deaminase(ADA)has highly differential diagnosis value and can be as supplementary method to diagnose tuberculous hydrothorax.

Objective To evaluate the Cys-C、β~2-MG、RBP as marker三of early diagnosis in patients with diabetic nephropathy Ⅱ(DN).Methods ninety patients with DN were divided into three groups according to urinary albumin excretion rate (UAER),that is normal,micro and mass group.Serum Cys-C、β~2-MG、RBP、BUN,Crea were detected and parallelly contrasted in the three groups and control group with thirty healthy people.Results compared with the control group,the serum Cys-C、β~2-MG、RBP of micro and mass group both significantly increased and the normal group had no significant difference.The positive rate of Cys-C、β~2-MG,RBP in mass group was respectively 100%、100%、96.1%;the positive rate in micro group as follows:Cys C (92.3%)＞B2-MG(84.6%)＞RBP(66.6%)＞Crea(46.1%)＞BUN(28.2%).Conclusion serum Cys-C、β~2-MG、RBP have high positive rates in patients with diabetic nephropathy Ⅱ(DN).They can be used as early diagnostic marker of diabetic nephropathy Ⅱespecially Cys-C.

Objective Discusses TT3,FT3,TT4,FT4 and TSH the value which examines in the thyroid gland disease patient；analysis thyroid gland disease patient TT3,FT3,TT4,FT4 and TSH change situation.Methodsanalyzes my hospital 200 example thyroid gland disease patients,and selects the normal adult 50 examples to take the control group.analytic method to each group of examination thyroid function,and carries on the comparison to the group various targets'examination value.Results primary hyperthyroidism group T3,T4,FT3,FT4 were higher than normally,the hypothyroidism group T3,T4,Fr3,FT4 to be lowerthan normally.The TSH content primary hyperthyroidism group was lower than normally,the hypothyroidism group to be higher than normally obviously.Compares its difference with the control group to have statistics significance.hyperthyroidism group its diagnosis coincidence rate FT3 was 96%,TSH was 96%,T3 was 92%,FT4 was 90%,T4 was 88%.The hypothyroidism,the diagnosis coincidence rate respectively was：TSH was 100%,FT4 was 93%,T4 was 90%,FT3 was 77%,T3 was 70%.Conclusion FT3,T3,TSH the significance are big in the hyperthyroidism diagnosis；FT4,T4,TSH the significance are big in the hypothyroidism diagnosis.

Objective Discusses TT3,FT3,TT4,FT4 and TSH the value which examines in the thyroid gland disease patient;analysis thyroid gland disease patient TT3,FT3,TT4,FT4 and TSH change situation.Methods analyzes my hospital 200 example thyroid gland disease patients,and selects the normal adult 50 examples to take the control group.analytic method to each group of examination thyroid function,and carries on the comparison to the group various targets'examination value.Results primary hyperthyroidism group T3,T4,FT3,FT4 were higher than normally,the hypothyroidism group T3,T4,Fr3,FT4 to be lower than normally.The TSH content primary hyperthyroidism group was lower than normally,the hypothyroidism group to be higher than normally obviously.Compares its difference with the control group to have statistics significance.hyperthyroidism group its diagnosis coincidence rate FT3 was 96%,TSH was 96%,T3 was 92%,FT4 was 90%,T4 was 88%.The hypothyroidism,the diagnosis coincidence rate respectively was:TSH was 100%,FT4 was 93%,T4 was 90%,FT3 was 77%,T3 was 70%.Conclusion FT3,T3,TSH the significance are big in the hyperthyroidism diagnosis;FT4,T4,TSH the significance are big in the hypothyroidism diagnosis.