No abstract available.
B-Lymphocytes* ; Humans* ; Interleukins*

No abstract available.
B-Lymphocytes* ; Humans* ; Interleukins*

Study on cloning of the gene encoding human interleukin-2 with gene produce method by RT-PCR. Results: cloned and determine order of gene fragment encoded IL-2 that having 333pp size (133 amino acids) from total RNA of human spleen by RT-PCR. The results of analysis order of cloned IL2 gem showed that there were 2 changes of single nucleotide (A36G, G54T) in comparison with that of the NP-000577 order registered in NCBI GenBank A36G and G54T. However, these changes did not make changes in amino acid sequence of the cloned II-2 gene
Interleukins ; Human ; Genes

No abstract available.
Antibodies, Monoclonal* ; Humans* ; Interleukins*

Having an understanding of the properties of cytokines is essential for the immunologist, the researcher and the medical practitioner who need to understand immunologic diseases and immunological therapeutic approaches. Cytokines are redundant in their actions on target cells and promiscuous in their receptor reactions. (ED note: That is some cool use of English!) Moreover, many cells concomitantly produce several cytokines that have overlapping actions. Here this review provides conceptual framework to understand the intriguing aspects of the cytokine system.
Cytokines ; Immune System Diseases ; Interleukins

OBJECTIVE: To explore the expression of regulatory T cells (Tregs) and interleukin-35 (IL-35) in peripheral blood of patients with newly diagnosed acute myeloid leukemia (AML). METHODS: Peripheral blood samples were collected from 33 newly diagnosed AML patients and 40 healthy volunteers. The levels of innate and adaptive immune cells and Tregs were detected by flow cytometry. Plasma IL-35 level was detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of P35 and EBI3 mRNA were detected by RT-PCR. The correlation between the levels of Tregs and IL-35 was analyzed. RESULTS: The proportion of CD4⁺T cells, CD8⁺T cells, B cells and NK cells in peripheral blood of newly diagnosed AML patients were significantly lower than those of control group (P<0.05). The levels of Tregs, IL-35, and the expression of P35 mRNA and EBI3 mRNA were significantly higher than that of healthy control group (P<0.01). The Tregs level was positively correlated with plasma IL-35 level (r=0.668,P<0.01). CONCLUSION AML patients have a deficiency in immune function, which is characterized by the high levels of Tregs and IL-35 in peripheral blood, and it might be a new target for the treatment of AML.
Humans ; Interleukins ; Leukemia, Myeloid, Acute

No abstract available.
Ascitic Fluid* ; Endometriosis* ; Female ; Humans ; Interleukins*

No abstract available.
Interleukin-2* ; Interleukins* ; Killer Cells, Natural*

Humans ; Interleukin-33 ; Interleukins ; Liver Cirrhosis

Interleukins (ILs) and associated cytokines serve as the means of communication for immune cells and non-immune cells. The use of ILs in harnessing the immune system to cancer treatment has been a promising approach. ILs not only nurture an environment enabling cancer growth but also simultaneously trigger a productive tumor-directed immune response. These properties of ILs are increasingly being explored as a strategy to improve the outcomes of cancer. Here, we describe recently innovative technological approaches that have been developed to improve the pharmacokinetics, safety, and efficacies of IL-2, 15, 10, and 18 in the treatment of melanoma. Furthermore, the combination of ILs and immune checkpoint inhibition may synergize to reshape the tumor environment, thus yielding better clinical benefits in the future.
Cytokines ; Humans ; Interleukins ; Melanoma/drug therapy*