1.Construction of recombinant fowlpox virus expressing chicken IL-2 and assay of biologic activity of the product in vitro.
Wei-Xing SHAO ; Da-Xin PENG ; Jian-Hong LU ; Dong-Ping WEI ; Yu-Liang LIU ; Xiu-Fan LIU
Chinese Journal of Biotechnology 2004;20(1):136-139
In order to determine the adjuvant effects of the chicken IL-2 (ChIL-2) on new generation vaccines, ChIL-2 gene was amplified from ConA-stimulated chicken spleen cells by RT-PCR and was directionally inserted into fowlpox virus (FPV) transferring vector p1175 under the control of FPV early/late promoter (PE/L), resulting in recombinant transferring vector p1175IL2. Then the p1175IL2 plasmid was transfected into chicken embryo fibroblasts (CEF) pre-infected with wild type FPV to generate recombinant fowlpox virus expressing ChIL-2 (rFPV-IL2). By selection of blue plaques on the CEF, overlaid with agar containing X-gal, rFPV-IL2 was obtained and purified. The supernatant from CEF monolayer infected with rFPV-IL2 (M.O.I2.0) after 72 hours was detected for the production of ChIL-2 by XTT/PMS colorimetric assay. About 3.6 x 10(5) u/mL of specific ChIL-2 activity was determined. The results show that rFPV-IL2 can express ChIL-2 effectively. rFPV-IL2 provides us with an effective tool for studying avian immunology as well as a potential vaccine-enhancing agent.
Animals
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Chick Embryo
;
Chickens
;
Fowlpox virus
;
genetics
;
Interleukin-2
;
genetics
;
pharmacology
;
Recombinant Proteins
;
biosynthesis
;
pharmacology
2.Effects of humanized recombinant CD25 monoclonal antibody on activation and proliferation of t lymphocytes.
Journal of Experimental Hematology 2007;15(1):134-137
The study was purposed to investigate the effects of humanized recombined CD25 monoclonal antibody (rhCD25MAb) on activation and proliferation of T lymphocytes in vitro. Peripheral blood mononuclear cells (PBMNC) were incubated with phytohemagglutinin (PHA). Before or after T cell activation, the cells were cultured with or without rhCD25MAb or cyclosporine A (CsA) in vitro. After 72 hours incubation, the proliferation of lymphocytes was analyzed by MTT assay. The expression of CD3 and CD25 antigens on T lymphocytes were detected by flow cytometry. The levels of sIL-2R in the supernatants were determined with ELISA. The results showed that both rhCD25MAb and CsA could inhibit the proliferation of T lymphocytes significantly in concentration-dependent manner and CsA was more efficient than rhCD25MAb. Both rhCD25MAb and CsA could also decrease the levels of sIL-2R in the supernatant and inhibit the expression of CD25 antigen on T lymphocytes. The level of sIL-2R and the expression of CD25 on T lymphocytes decreases more profoundly in rhCD25MAb group. It is concluded that rhCD25MAb shows strong immunosuppressive activity both before and after T cell activation, suggesting that this agent may be useful in not only prophylaxis but also the treatment of acute graft-versus-host disease.
Antibodies, Monoclonal
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pharmacology
;
CD3 Complex
;
biosynthesis
;
genetics
;
Cell Proliferation
;
Cells, Cultured
;
Cyclosporine
;
pharmacology
;
Humans
;
Immunosuppressive Agents
;
pharmacology
;
Interleukin-2 Receptor alpha Subunit
;
biosynthesis
;
genetics
;
immunology
;
Lymphocyte Activation
;
immunology
;
Receptors, Interleukin-2
;
analysis
;
Recombinant Proteins
;
immunology
;
pharmacology
;
T-Lymphocytes
;
immunology
3.Effects of entrapment of murine interleukin-2 gene with chitosan nanoparticles on expression of mIL-2 gene and on regulation of immune response in mice.
Hui LI ; Rong GAO ; Mei WU ; Manliang FU ; Kaiyuan WU ; Yi YANG ; Xiuying WANG ; Zezhou WANG ; Shigui LIU
Journal of Biomedical Engineering 2004;21(6):947-952
The experiment was conducted to prepare chitosan nanoparticles (CNP), to entrap VRMIL-2 with CNP, the eukaryotic VR1020 expression plasmid containing murine IL-2 gene (mlL-2), and to investigate the expression in vivo and the regulatory effect of mIL-2 on immune-response and immuno-protection in mice inoculated muscularly with CNP entrapped VMIL-2 at 21 days old. The results showed that IgG, IgM and IgA contents increased to different degrees in the sera from the inoculated mice, which were remarkably higher than those of the controls inoculated VR1020 packed with CNP (P<0.05); so were the IL-2, IL-4 and IL-6 contents in the sera of the immunized mice. The number of white blood cells and lymphocytes significantly increased respectively in the vaccinated mice, compared with those of controls. These mice were orally challenged with virulent E. coli 35 days post-inoculation, and all the immune responses were significantly higher than those of the control except the number of neutrophils. The mice inoculated with VRMIL-2 survived healthily, while the mice of control group were ill with the evident lesions. Although there are no remarkable differences between the cellular and humoral immune indexes of mice inoculated with CNP-VRMIL-2 and nude VRMIL-2 (P>0.05), the dosage of CNP-VRMIL-2 is only one fifth of the VRMIL-2. These indicated that entrapment of mIL-2 gene with chitosan nanoparticles could remarkably enhance the expression of mIL-2 in vivo, and significantly raise the levels of cellular and humoral immune, and increase the resistance of mice against E. coli infection. The results suggested that chitosan nanoparticles and IL-2 gene could be used as an effective immunoenhancer to increase the immunity of animals against infection.
Adjuvants, Immunologic
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pharmacology
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Animals
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Chitosan
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pharmacology
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Escherichia coli Infections
;
immunology
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prevention & control
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Female
;
Immunoglobulin G
;
blood
;
Interleukin-2
;
biosynthesis
;
genetics
;
pharmacology
;
Mice
;
Nanostructures
4.Ginsenoside-Ro enhances cell proliferation and modulates Th1/Th2 cytokines production in murine splenocytes.
Jun-li YU ; De-qiang DOU ; Xiao-hong CHEN ; Hong-zhen YANG ; Xiao-yan HU ; Gui-fang CHENG
Acta Pharmaceutica Sinica 2005;40(4):332-336
AIMTo study the effects of ginsenoside-Ro on cell proliferation and cytokine production in murine splenocytes.
METHODSThe effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3H] thymidine incorporation assay. Effects of ginsenoside-Ro on the production of cytokines interleukin-2 (IL-2), interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) from murine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of Th1 cytokine IFN-gamma and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis.
RESULTSGinsenoside-Ro showed no mitogenic effect on unstimulated murine splenocytes. It enhanced the proliferation of Con A-induced murine splenocytes and the production of IL-2 at concentrations of 1-10 micromol x L(-1). Moreover, ginsenoside-Ro increased the production and expression of Th2 cytokine IL-4 and decreased the production and expression of Th1 cytokine IFN-gamma in Con A-induced murine splenocytes at concentrations of 2-10 micromol x L(-1).
CONCLUSIONGinsenoside-Ro showed immunomodulatory effects by regulating the production and expression of Th1/Th2 cytokines in murine splenocytes.
Animals ; Cell Proliferation ; drug effects ; Ginsenosides ; isolation & purification ; pharmacology ; Interferon-gamma ; biosynthesis ; genetics ; Interleukin-2 ; metabolism ; Interleukin-4 ; biosynthesis ; genetics ; Male ; Mice ; Mice, Inbred BALB C ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; RNA, Messenger ; biosynthesis ; genetics ; Spleen ; cytology ; metabolism
5.Effect of tramadol on immune responses and nociceptive thresholds in a rat model of incisional pain.
Yong-Min LIU ; Sheng-Mei ZHU ; Kui-Rong WANG ; Zhi-Ying FENG ; Qing-Lian CHEN
Journal of Zhejiang University. Science. B 2008;9(11):895-902
OBJECTIVETo evaluate the effects of tramadol on the proinflammatory responses in a rat model of incisional pain by investigating its effects on nociceptive thresholds and serum interleukin-6 (IL-6) and IL-2 levels.
METHODSForty-two male Sprague-Dawley (SD) rats scheduled for plantar incision were randomly divided into 7 groups (n=6 in each group). Rats in Group 1 receiving general anesthesia with no incision were served as control; At 30 min before skin incision, Groups 2 to approximately 5 were given 5 ml normal saline or 1, 10, and 20 mg/kg tramadol, respectively, intraperitoneally (i.p.); Group 6 received 10 mg/kg tramadol after operation; Group 7 received 10 mg/kg tramadol before incision, followed by 200 microg/kg naloxone after operation. Mechanical allodynia was measured by electronic von Frey filament to evaluate the nociceptive thresholds 1 h before incision, and 1 h and 2 h after operation. Serum IL-6 and IL-2 levels were measured by enzyme-linked immunosorbent assay (ELISA) 2 h after operation.
RESULTSMechanical thresholds decreased significantly and serum IL-6 level increased significantly after operation in Group 2 compared with control (P<0.01), and these changes were reversed respectively by tramadol in a dose-dependent manner (P<0.05 and P<0.01, respectively). IL-2 level remained unchanged after operation in Group 2, but decreased in Group 3 (P<0.05), then gradually returned to the normal level in Groups 4 and 5. The intraperitoneally injected tramadol (10 and 20 mg/kg) produced a potent and dose-dependent antinocicptive effect on the lesioned paw. The antinocicptive effects of tramadol were partially antagonized by naloxone (200 microg/kg), suggesting an additional non-opioid mechanism.
CONCLUSIONThe results suggest that tramadol could be a good choice for the treatment of pain under the conditions that immunosuppression may be particularly contraindicated.
Analgesics, Opioid ; pharmacology ; Animals ; Dose-Response Relationship, Drug ; Interleukin-2 ; biosynthesis ; blood ; Interleukin-6 ; biosynthesis ; blood ; Male ; Pain Measurement ; methods ; Pain Threshold ; drug effects ; Pain, Postoperative ; blood ; drug therapy ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Tramadol ; pharmacology
6.Effects of IL-10 and PDGF-BB on expression of EGF and bFGF mRNA in hepatic stellate cells.
Yue-Hong HUANG ; Xiao-Zhong WANG ; Yun-Xin CHEN ; Zhi-Xin CHEN ; Li-Juan ZHANG
Chinese Journal of Hepatology 2004;12(9):562-563
Cell Line
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Epidermal Growth Factor
;
biosynthesis
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genetics
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Fibroblast Growth Factor 2
;
biosynthesis
;
genetics
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Hepatocytes
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metabolism
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Humans
;
Interleukin-10
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pharmacology
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Platelet-Derived Growth Factor
;
pharmacology
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Proto-Oncogene Proteins c-sis
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RNA, Messenger
;
biosynthesis
;
genetics
7.Effect of polyadenylic.polyuridylic acid on the proliferative responsiveness of mouse thymus and spleen cells.
Bong Ki LEE ; Youn Jung YU ; Jung Koo YOUN
Yonsei Medical Journal 1990;31(2):174-181
The effects of polyadenylic.polyuridylic acid [poly(A).poly(U)] on in vitro proliferations of thymus and spleen cells from C57BL/6 mice were investigated. Mice were injected intravenously with 30 micrograms of poly(A).poly(U) or placebo. Two days later, thymus, spleen and peritoneal cells from these mice were prepared and cultured in pooled or non-pooled conditions. Cell proliferations were assessed by the technique of incorporation of tritiated thymidine. It has been revealed that the in vitro proliferations of thymus and spleen cells as well as the productions of interleukin-1 by peritoneal adhering cells and interleukin-2 by spleen cells were significantly enhanced in the cultures of cells from poly(A).poly(U)-treated mice. These enhancing effects were observed only in the cultures of pooled cells from mice whose genetic homogeneity is suspected. Furthermore, thymus cells from poly(A).poly(U)-treated mice acted as strong responder cells but not as stimulators in one way mixed cultures. Thus, the enhanced cellular responsiveness may be mediated by the increased production of cytokines and antigen recognitions of thymus-derived cells following activations via the adjuvant effect of poly(A).poly(U).
Animal
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Cell Division/drug effects
;
Cells, Cultured
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Female
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Interleukin-1/biosynthesis
;
Interleukin-2/biosynthesis
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Male
;
Mice
;
Mice, Inbred C57BL
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Poly A-U/administration & dosage/*pharmacology
;
Spleen/*cytology
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Support, Non-U.S. Gov't
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Thymus Gland/*cytology
8.In vitro effect of interleukin-2 on proliferative responses of peripheral blood T cells from leprosy patients.
In Hong CHOI ; Jeon Soo SHIN ; Joo Deuk KIM ; Se Jong KIM
Yonsei Medical Journal 1991;32(3):237-242
Because of the important role played by interleukin-2(IL-2) in T cell growth and differentiation, we investigated the effect of exogenous IL-2 on the proliferative response of peripheral blood mononuclear cells(PBMCs) from 77 leprosy patients. The proliferative responses of PBMCs from lepromatous leprosy(LL) or borderline lepromatous leprosy(BL) patients to M. leprae were significantly lower(cpm 6,051 +/- 803 for LL type; 4,951 +/- 2,529 for BL type) than those from tuberculoid leprosy(TT) or borderline tuberculoid leprosy(BT) patients (28,853 +/- 28,916 for TT type; 15,884 +/- 334 for BT type). To investigate the effect of exogenous IL-2, purified IL-2 was added at the start of culture at 100 unit/ml. There was an apparent increase in 3H-thymidine incorporation of M. leprae-stimulated PBMCs(18,723 +/- 6,503) in the presence of IL-2 compared to the results without IL-2(6,051 +/- 803) in LL patients. Twenty nine out of 33 LL patients belonged to the responders to IL-2 and four patients were nonresponders. Therefore we conclude that the defective cell mediated immune response in LL patients may result from diminished production of IL-2, but we can not exclude the possibility of diminished expression of the IL-2 receptor. And we suggest that the immunologic heterogeneous response of an individual to M. leprae is important to the pathogenesis of clinical disease in the same LL patients.
Cells, Cultured
;
Comparative Study
;
Human
;
Immunity, Cellular
;
Interleukin-2/biosynthesis/*pharmacology
;
Leprosy/blood/*immunology
;
Leukocytes, Mononuclear
;
Lymphocyte Activation
;
Mycobacterium leprae/immunology
;
Support, Non-U.S. Gov't
;
T-Lymphocytes/*immunology
9.Anti-CD47 monoclonal antibody (B6H12) impairs the maturation and function of human dendritic cells.
Journal of Experimental Hematology 2005;13(2):192-197
To investigate the influence and mechanisms of CD47 engagement by its soluble mAb B6H12 on the maturation and function of cultured dendritic cells (DCs), monocyte-derived DCs were propagated in granulocyte-macrophage colony stimulating factor (GM-CSF) combined with lipopolysaccharide (LPS) and interleukin (IL)-4, in the presence or absence of soluble anti-CD47 monoclonal antibodies (anti-CD47 mAbs, B6H12). The characteristic morphology of DCs was identified by using the transmission electron microscopy. Flow cytometry was used to detect the cell surface phenotypes. The concentration of IL-12 P70 in supernatant was measured by ELISA. The antigen-presenting functions of DCs were determined in one-way mixed leukocyte reaction by BrdU-ELISA. Electrophoretic mobility shift assay (EMSA) was applied to examine the activity of NF-kappaB. The results indicated that the anti-CD47 mAbs markedly suppressed the expression of CD80, CD86, CD83, CD1a and HLA-DR on the surface of DCs (P < 0.05). The data of the mixed leukocyte reaction and IL-12 P70 production were consistent with the results by flow cytometry (P < 0.01). Pre-exposure to B6H12 mAb during the development of DCs resulted in a dramatic depletion of the DNA binding activity of NF-kappaB toward nucleus protein. Moreover, such an inhibition effect seemed to be dose dependent. In conclusion, the soluble mAb B6H12 inhibits the expression of the costimulatory molecules and MHCII molecules on the DCs. The antigen-presenting function of DCs was also impaired by B6H12. And these modulations are closely related with the depletive DNA binding activity of NF-kappaB. It is suggested that the soluble B6H12 exerts a negative effect on the maturation and function of in vitro cultured DCs due to inhibition of NF-kappaB binding activity.
Antibodies, Monoclonal
;
immunology
;
pharmacology
;
Antigens, CD
;
biosynthesis
;
Antigens, CD1
;
biosynthesis
;
B7-1 Antigen
;
biosynthesis
;
B7-2 Antigen
;
biosynthesis
;
CD47 Antigen
;
immunology
;
Cell Size
;
drug effects
;
Cells, Cultured
;
Dendritic Cells
;
cytology
;
drug effects
;
metabolism
;
Electrophoretic Mobility Shift Assay
;
Enzyme-Linked Immunosorbent Assay
;
Flow Cytometry
;
HLA-DR Antigens
;
biosynthesis
;
Humans
;
Immunoglobulins
;
biosynthesis
;
Interleukin-12
;
metabolism
;
Membrane Glycoproteins
;
biosynthesis
;
NF-kappa B
;
metabolism
;
Oligonucleotides
;
metabolism
;
Protein Binding
10.Effect of peroxisome proliferator-activated receptor-alpha agonist on adipokines expression in rats fed with high-fat diet.
Yan LI ; Bin HUANG ; Hua CHENG ; Zhen LIANG ; Shan-ying LIU
Acta Academiae Medicinae Sinicae 2006;28(6):761-765
OBJECTIVETo explore the effect of peroxisome proliferator-activated receptor-alpha ( PPAR-alpha) agonist fenofibrate on adipokines expression in high-fat diet fed SD rats and its relationship to insulin resistance (IR).
METHODSRats were randomized into three groups (n = 10) : HD group, fed with high-fat diet; HDF group, fed with high fat diet and treated with fenofibrate; and control group, fed with normal diet. Animals were sacrificed after 4-week follow-up. Plasma lipids, fasting plasma insulin, free fatty acids (FFA), and insulin sensitivity were detected. Reverse transcription-polymerase chain reaction was used to semi-quantitatively determine the mRNA expression of adipokines including tumor necrosis factor-alpha (TNF-alpha) , interleukin-6 (IL-6), angiotensinogen (AGT), angiotensin 11 type 1 receptor (AT1R), and adiponectin in brown fat.
RESULTSThe plasma level of FFA, TG, and homeostatic model approach-IR index were (2. 37+/-0. 60) vs (1. 59+/-0. 30) vs (1. 33+/-0. 34 ) mmol/L, (0. 48+/-0. 11) vs (0. 30+/-0. 04) vs (0. 36+/-0. 07) mmol/L, and 12. 30+/-3. 97 vs 5. 03 +/-1. 88 vs 4. 17+/-1. 27 in the HD group, HDF group, and control group after 4 weeks of treatment with fenofibrate, respectively. The mRNA expressions of TNF-alpha and adiponectin were 1. 726+/-1. 408 vs 0. 713+/-0. 711 vs 0. 593+/-0. 382 and 0. 660+/-0. 192 vs 0. 949+/-0. 35 vs 0. 936+/-0. 130 in these three groups, which showed significant difference between HD group and HDF group (P < 0. 05 ) , while no significant difference between HDF group and control group (P > 0. 05). The mRNA expressions of AGT, AT1 R, and IL-6 had no significant difference among these three groups (P > 0. 05 ).
CONCLUSIONPPAR-alpha agonist fenofibrate may reverse high-fat diet induced lipid abnormalities, improve insulin sensitivity, and regulate the mRNA expressions of TNF-alpha and adiponectin in adipose tissues.
Adiponectin ; biosynthesis ; Adipose Tissue ; drug effects ; metabolism ; Angiotensins ; biosynthesis ; Animals ; Dietary Fats ; adverse effects ; Disease Models, Animal ; Fenofibrate ; pharmacology ; Insulin Resistance ; Interleukin-6 ; biosynthesis ; Lipofuscin ; biosynthesis ; Male ; PPAR alpha ; antagonists & inhibitors ; RNA, Messenger ; biosynthesis ; Rats ; Rats, Sprague-Dawley ; Receptor, Angiotensin, Type 2 ; biosynthesis ; Tumor Necrosis Factor-alpha ; biosynthesis