1.Partial purification and characterization of a novel murine factor that augments the expression of class I MHC antigens on tumor cells.
Experimental & Molecular Medicine 1998;30(2):93-99
A soluble factor which augments the expression of major histocompatibility complex class I (MHC I) antigens on a number of murine tumor cell lines, has been isolated from the culture supernatants of mixed lymphocyte reaction of spleen cells derived from C57B1/6, Balb/c and Swiss mice. The factor, termed MHC-augmenting factor (MHC-AF) has been partially purified by Sephadex G-100 column chromatography and reverse phase HPLC. MHC-AF activity is associated with an 18 kDa molecule. MHC-AF activity was resistant to pH 2.0 treatment and partially purified MHC-AF preparations did not have any activity in L929 cell/vesicular stomatitis virus (VSV) interferon bioassay system. Antibodies to IFN-gamma did not block the activity of MHC-AF. These results indicate that a MHC-AF distinct from IFN-gamma, is produced by mouse spleen cells undergoing a mixed lymphocyte reaction.
Animal
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Antibodies/pharmacology
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Chymotrypsin/metabolism
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Chymotrypsin/chemistry
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Comparative Study
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Concanavalin A/pharmacology
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Heat
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Histocompatibility Antigens Class I/metabolism*
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Histocompatibility Antigens Class I/drug effects
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Interferon Type II/pharmacology
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Interferon Type II/metabolism
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Interferon Type II/immunology
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Lymphocytes/physiology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Proteins/pharmacology*
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Proteins/metabolism
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Proteins/isolation & purification*
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Spleen/cytology
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Trypsin/metabolism
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Trypsin/chemistry
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Tumor Cells, Cultured/immunology
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Tumor Cells, Cultured/drug effects
2.Modulation of the Surface Expression of CD158 Killer Cell Ig-like Receptor by Interleukin-2 and Transforming Growth Factor-beta.
Eui Cheol SHIN ; Kyung Seon CHOI ; Se Jong KIM ; Jeon Soo SHIN
Yonsei Medical Journal 2004;45(3):510-514
Killer cell Ig-like receptor (KIR) binds to HLA class I molecules on the surface of target cells, and it confers inhibitory signals to NK cells. Although NK cytotoxicity can be affected by the change of the surface expression of KIR on NK cells, the effect of cytokines on the regulation of KIR expression has not been thoroughly investigated. Here in our study, we investigated the effect of several cytokines, including IL-2, TGF-beta, IFN-gamma, IL-12 and IL-18, on the surface expression of CD158 KIR, which binds to HLA-C, by the use of FACS analysis. In the isolated NK cells, IL-2 obviously increased the surface expression of CD158 KIR after 72 hr in vitro culture, and this was evidenced by the increased percentage of CD158+ NK cells and the increased mean fluorescence intensity of CD158 in CD158+ NK cells. In contrast, TGF-beta decreased the surface expression of CD158 KIR after 72 hr culture. However, IFN-gamma, IL-12 and IL-18 did not change the expression of CD158 KIR. The modulated expression of KIR by IL-2 and TGF-beta can be associated with the changed NK-cytotoxic target-discriminating ability of NK cells upon their exposure to IL-2 and TGF-beta.
Antineoplastic Agents/*pharmacology
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Cells, Cultured
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Human
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Interferon Type II/pharmacology
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Interleukin-12/pharmacology
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Interleukin-18/pharmacology
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Interleukin-2/*pharmacology
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Killer Cells/cytology/*drug effects/*metabolism
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Receptors, Immunologic/*metabolism
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Support, Non-U.S. Gov't
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Transforming Growth Factor beta/*pharmacology
3.Phagocytosis of serum-and IgG-opsonized zymos an particles induces apoptosis through superoxide but not nitric oxide in macrophage J774A.1.
Jun Sub KIM ; Hyeok Yil KWON ; Won Ho CHOI ; Chan Young JEON ; Jong Il KIM ; Jaebong KIM ; Jae Yong LEE ; Yong Sun KIM ; Jae Bong PARK
Experimental & Molecular Medicine 2003;35(3):211-221
Phagocytosis of serum- and IgG-opsonized zymosan (SOZ and IOZ, respectively) particles into J774A.1 macrophages induced apoptosis of the cells, accompanied by the expression of p21(WAF1), one of cyclin-dependent protein kinase (CDK) inhibitors. Furthermore, phagocytosis of SOZ and IOZ particles into macophages induced superoxide formation. Tat-superoxide dismutase (SOD), which is readily transduced into the cells using Tat-domain, protected the cells from the apoptosis induced by phagocytosis of SOZ and IOZ particles. lipopolysaccharide (LPS)/interferon-gamma (IFN-gamma) also caused the apoptosis of the cells. However, Tat-SOD could not protect the cells from LPS/IFN-gamma induced apoptosis, suggesting that apoptosis mechanisms involved are different from each other. In the present study, we determined the amounts of nitric oxide (NO) produced by SOZ, IOZ, and LPS/IFN-gamma, and found that SOZ and IOZ did not induce the generation of NO in macrophages, whereas LPS/ IFN-gamma did. The apoptosis due to phagocytosis was accompanied with the release of cytochrome c from mitochondrial membrane to cytosolic fraction. Furthermore, SOZ and IOZ induced the cleavage of procasapase-3 (35 kDa) to give rise to an active caspase-3 (20 kDa), which was blocked by Tat- SOD but not by 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO), a scavenger of NO. On the other hand, LPS/IFN-gamma caused the activation of procaspase-3, which was blocked by PTIO but not by Tat-SOD. Taken together, phagocytosis of SOZ and IOZ particles induced apoptosis through superoxide but not NO in macrophages, accompanied with the release of cytochrome c and the activation of caspase-3.
Apoptosis/*immunology
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Caspases/metabolism
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Cell Line
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Cyclins/biosynthesis
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Cytochromes c/metabolism
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Immunoglobulin G/*immunology
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Interferon Type II/pharmacology
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Lipopolysaccharides/pharmacology
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Macrophages/*immunology/metabolism
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Nitric Oxide/*metabolism
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Opsonins/immunology
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Phagocytosis/*physiology
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Superoxide Dismutase/metabolism
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Superoxides/*metabolism
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Zymosan
4.Induction of ICAM-1 and HLA-DR expression by IFN-gamma in malignant melanoma cell lines.
Joo Deuk KIM ; Jung Lim LEE ; Jeon Han PARK ; Jae Myun LEE ; Yeon Hyang KIM ; Se Jong KIM
Yonsei Medical Journal 1995;36(1):15-25
Two human malignant melanoma cell lines, Malme-3M and SK-Mel-28, were analyzed for their ability to induce the expression of intercellular adhesion molecule 1 (ICAM-1) and human leukocyte antigen (HLA)-DR molecules on their cell surfaces as well as at the transcriptional level before and after treatment with interferon (IFN)-gamma. Both cell lines demonstrated a high percentage(> 99%) of ICAM-1 expression regardless of IFN-gamma treatment. Before IFN-gamma treatment, Malme-3M cells barely expressed HLA-DR molecules (< 2%) and SK-Mel-28 cells demonstrated a relatively high percentage(> 50%) of HLA-DR expression. Both cell lines displayed elevated levels of HLA-DR expression in a time dependent manner after IFN-gamma treatment. However, these two cell lines have been shown to respond differentially to IFN-gamma. The molecular mechanism underlying such a differential behavior was investigated, and HLA-DR gene regulation was studied at the transcriptional level. Treatment with IFN-gamma led to the steady-state mRNA augmentation of the HLR-DR gene. The HLA-DRA mRNA augmentation was similar in both cell lines, whereas in Malme-3M, IFN-gamma did not augment the rate of transcription of the HLA-DRB gene as much as in SK-Mel-28. Data from this study established the fact that the melanoma cell lines displayed a differential susceptibility to IFN-gamma on the modulation of HLA-DR molecules, and this modulation was transcriptionally regulated.
Genes, MHC Class II
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HLA-DR Antigens/*metabolism
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Human
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Intercellular Adhesion Molecule-1/*metabolism
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Interferon Type II/*pharmacology
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Melanoma/*metabolism/pathology
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Support, Non-U.S. Gov't
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Transcription, Genetic
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Tumor Cells, Cultured
5.Human Brain Astrocytes Mediate TRAIL-mediated Apoptosis after Treatment with IFN-gamma.
Jeonggi LEE ; Jeon Soo SHIN ; In Hong CHOI
Yonsei Medical Journal 2006;47(3):354-358
TNF-related apoptosis inducing ligand (TRAIL) expressions were studied in primary human brain astrocytes in response to pro-inflammatory cytokines. When astrocytes were treated with IL-1beta TNF-alphaor IFN-gamma TRAIL was induced in cultured fetal astrocytes. In particular, IFN-gammainduced the highest levels of TRAIL in cultured astrocytes. When astrocytes were pre-reated with IFN-gamma they induced apoptosis in TRAIL-sensitive Peer cells. Our results suggest that IFN-gamma modulates the expression of TRAIL in astrocytes, which may enhance cytotoxic sensitivity of infiltrating immune cells or brain cells other than astrocytes during inflammation of brain.
Tumor Necrosis Factor-alpha/genetics/*metabolism
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TNF-Related Apoptosis-Inducing Ligand
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Membrane Glycoproteins/genetics/*metabolism
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Interferon Type II/*pharmacology
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Humans
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Cells, Cultured
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Astrocytes/*cytology/drug effects/metabolism
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Apoptosis Regulatory Proteins/genetics/*metabolism
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Apoptosis/*drug effects/physiology
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Antineoplastic Agents/*pharmacology
6.Human Brain Astrocytes Mediate TRAIL-mediated Apoptosis after Treatment with IFN-gamma.
Jeonggi LEE ; Jeon Soo SHIN ; In Hong CHOI
Yonsei Medical Journal 2006;47(3):354-358
TNF-related apoptosis inducing ligand (TRAIL) expressions were studied in primary human brain astrocytes in response to pro-inflammatory cytokines. When astrocytes were treated with IL-1beta TNF-alphaor IFN-gamma TRAIL was induced in cultured fetal astrocytes. In particular, IFN-gammainduced the highest levels of TRAIL in cultured astrocytes. When astrocytes were pre-reated with IFN-gamma they induced apoptosis in TRAIL-sensitive Peer cells. Our results suggest that IFN-gamma modulates the expression of TRAIL in astrocytes, which may enhance cytotoxic sensitivity of infiltrating immune cells or brain cells other than astrocytes during inflammation of brain.
Tumor Necrosis Factor-alpha/genetics/*metabolism
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TNF-Related Apoptosis-Inducing Ligand
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Membrane Glycoproteins/genetics/*metabolism
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Interferon Type II/*pharmacology
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Humans
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Cells, Cultured
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Astrocytes/*cytology/drug effects/metabolism
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Apoptosis Regulatory Proteins/genetics/*metabolism
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Apoptosis/*drug effects/physiology
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Antineoplastic Agents/*pharmacology
7.Effect of fengshiqing on interleukin-4, gamma-interferon and chemotactic factor in rats with collagen type II.
Wei CAO ; Quan JIANG ; Zhen-yu WU
Chinese Journal of Integrated Traditional and Western Medicine 2009;29(12):1114-1116
OBJECTIVETo study the regulatory effect of fengshiqing (FSQ) on interleukin 4 (IL-4), gamma-interferon (gamma-IFN), macrophage inflammatory protein 1alpha (MIP-1alpha) and collagen type II antibody (C II Ab) in serum and supernatant of synovial cell culture of rat with rheumatoid arthritis (RA), to explore the mechanism of action of clearing-heat and activating blood method for treatment of RA.
METHODSRA rat model was induced by C II Ab combined with Freund's complete adjuvant, and the levels of IL-4, gamma-IFN, MIP-1alpha and C II Ab in serum and supernatant of synovial cell were detected by ELISA.
RESULTSAs compared with the normal group, serum level of C II Ab in the model group was significantly higher (P < 0.01), serum and supernatant contents of IL-4 on the 14th and 28th day of modeling were lower and those of gamma-IFN and MIP-1alpha were higher, the difference showed statistical significance (P < 0.05 or P < 0.01). After being treated with FSQ and IL-4 contents in serum and supernatant as well as MIP-1alpha in supernatant restored on the 14th day (P < 0.05 or P < 0.01), while all the indexes restored on the 28th day.
CONCLUSIONFSQ could evidently up-regulate the level of IL-4 and down-regulate that of MIP-1alpha in serum and local synovial membrane in RA rats, and shows a suppressive trend of gamma-IFN, so as to maintain the Th1/Th2 equilibrium, suppress the cellular and humoral immune response in the local synovial membrane, and alleviate the chronic changes of arthritis, synovitis and vasculitis.
Animals ; Arthritis, Rheumatoid ; metabolism ; Chemokine CCL3 ; metabolism ; Collagen Type II ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Female ; Interferon-gamma ; metabolism ; Interleukin-4 ; metabolism ; Male ; Rats ; Rats, Wistar ; Th1-Th2 Balance
8.Receptor activator of NF-kappaB ligand enhances the activity of macrophages as antigen presenting cells.
Hyewon PARK ; Ok Jin PARK ; Jieun SHIN ; Youngnim CHOI
Experimental & Molecular Medicine 2005;37(6):524-532
Receptor activator of NFkappaB ligand (RANKL) is known as a key regulator of osteoclastogenesis. However, the fact that fibroblasts and periodontal ligament cells express RANKL in response to bacterial substances, suggests that RANKL may have evolved as a part of the immunity to infection. As RANKL increases the survival and activity of dendritic cells, it may have similar effects on macrophages. To address this issue, we studied the effect of RANKL on various functions of macrophages using mouse bone marrow derived macrophages. RANKL enhanced the survival of macrophages and up-regulated the expression of CD86. RANKL-treated macrophages showed increased allogeneic T cell activation and phagocytic activity compared to control cells. In addition, RANKL increased the expression of TNFalpha, MCP-1, and IL-6 but not of IL-10, IL-12, IFN-gamma, and iNOS. Collectively, RANKL augmented the activity of macrophages especially as antigen presenting cells, suggesting its new role in immune regulation.
Animals
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Antigen-Presenting Cells/cytology/*drug effects/immunology/*metabolism
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Antigens, CD86/metabolism
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Carrier Proteins/*pharmacology
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Cell Death/drug effects
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Cell Survival/drug effects
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Cells, Cultured
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Cytokines/metabolism
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Flow Cytometry
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Histocompatibility Antigens Class II/metabolism
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Inflammation Mediators
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Interferon Type II/pharmacology
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Lipopolysaccharides/pharmacology
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Macrophages/cytology/*drug effects/immunology/*metabolism
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Membrane Glycoproteins/*pharmacology
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Mice
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Mice, Inbred C57BL
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Mice, Inbred ICR
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Nitric Oxide Synthase Type II/metabolism
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Phagocytosis/drug effects
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Research Support, Non-U.S. Gov't
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T-Lymphocytes/immunology/metabolism
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Up-Regulation/drug effects/genetics
9.Human beta-defensin 2 is induced by interleukin-1b in the cornealepithelial cells.
Jun Seop SHIN ; Chan Wha KIM ; Young Sam KWON ; Jae Chan KIM
Experimental & Molecular Medicine 2004;36(3):204-210
Mammalian epithelia produce the various antimicrobial peptides against the bacterial or viral infection, thereby acting as the active immune modulators in the innate immunity. In this study, we examined the effects of the various proinflammatory cytokines or LPS on cell viability and antimicrobial beta-defensin gene expressions in human corneal epithelial cells. Results showed that the cytokines or LPS did not exert severe cytotoxic effects on the cells, and that beta-defensin 1 was constitutively expressed, while beta-defensin 2 was specifically induced by IL-1beta, supporting the idea that these cytokines or LPS involve the defense mechanism in the cornea. Furthermore, the reporter and gel shift assay to define the induction mechanism of beta-defensin 2 by IL-1beta demonstrated that the most proximal NF-kB site on the promoter region of beta-defensin 2 was not critical for the process. Data obtained from the normal or patients with the varying ocular diseases showed that our in vitro results were relevant in the clinical settings. Our results clearly demonstrated that beta-defensin 1 and 2 are important antimicrobial peptides in the corneal tissues, and that the mechanistic induction process of beta-defensin 2 by IL-1beta is not solely dependent on proximal NF-kB site activation, thus suggesting that the long distal portion of the promoter is needed for the full responsiveness toward IL-1beta.
Binding, Competitive
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Cell Survival
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Cells, Cultured
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Corneal Diseases/metabolism
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Electrophoretic Mobility Shift Assay
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Epithelium, Corneal/drug effects/*immunology/metabolism
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Gene Expression
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Humans
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Interferon Type II/metabolism/pharmacology
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Interleukin-1/*pharmacology
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Lipopolysaccharides/metabolism/pharmacology
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NF-kappa B/metabolism
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Promoter Regions (Genetics)/drug effects/genetics
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Research Support, Non-U.S. Gov't
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Tumor Necrosis Factor-alpha/metabolism/pharmacology
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beta-Defensins/*biosynthesis/genetics/metabolism
10.Divergent immunomodulatory effects of extracts and phenolic compounds from the fern Osmunda japonica Thunb.
Xiao-xin ZHU ; Yu-jie LI ; Lan YANG ; Dong ZHANG ; Ying CHEN ; Eva KMONICKOVA ; Xiao-gang WENG ; Qing YANG ; Zdeněk ZÍDEK
Chinese journal of integrative medicine 2013;19(10):761-770
OBJECTIVETo study possible immunobiological potential of Osmunda japonica Thunb.
METHODSImmunomodulatory effects of ethanol extracts prepared from rhizomes of O. japonica and phenolic compounds isolated from the extracts were investigated under the in vitro conditions using the rat peritoneal cells (2×10(6)/mL; 24 h culture). Biosynthesis of nitric oxide (NO) was assayed by Griess reagent, production of prostaglandin E2 (PGE2) and secretion of cytokines were determined by enzyme-linked immunoabsorbent assay.
RESULTSThe extracts activated dose dependently, with the onset at 2.5-5 μmol/L concentrations, the high output NO production, and secretion of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Mild enhancement of NO was produced by the aldehyde-type phenolics 4-hydroxybenzaldehyde and 3,4-hydroxybenzaldehyde. In contrasts, the acetone-type phenolics 4-hydroxybenzalacetone and 3,4-hydroxybenzalacetone inhibited production of immune mediators including cytokines (TNF-α, IL-1β, IL-6), NO, and PGE2. The 3,4-hydroxybenzalacetone was more effective than 4-hydroxybenzaldehyde. The IC50s estimates ranged within the interval of 5-10 μmol/L. No signs of cytotoxicity were observed up to the 50 μmol/L concentration of the compounds.
CONCLUSIONPhenolic compounds contained in medicinal herb Osmunda japonica possess distinct immunomodulatory activity.
Animals ; Cell Survival ; drug effects ; Cells, Cultured ; Dinoprostone ; biosynthesis ; Female ; Ferns ; chemistry ; Immunologic Factors ; pharmacology ; Interferon-gamma ; pharmacology ; Lipopolysaccharides ; pharmacology ; Nitric Oxide ; biosynthesis ; Nitric Oxide Synthase Type II ; genetics ; metabolism ; Peritoneum ; cytology ; drug effects ; Phenols ; chemistry ; isolation & purification ; pharmacology ; Plant Extracts ; chemistry ; isolation & purification ; pharmacology ; Polymyxin B ; pharmacology ; Proline ; analogs & derivatives ; pharmacology ; RNA, Messenger ; genetics ; metabolism ; Rats ; Rats, Wistar ; Thiocarbamates ; pharmacology