1.Antepartal insulin-like growth factor concentrations indicating differences in the metabolic adaptive capacity of dairy cows.
Marion PIECHOTTA ; Lars HOLZHAUSEN ; Marcelo Gil ARAUJO ; Maike HEPPELMANN ; Anja SIPKA ; Chistiane PFARRER ; Hans Joachim SCHUBERTH ; Heinrich BOLLWEIN
Journal of Veterinary Science 2014;15(3):343-352
Cows with different Insulin-like Growth Factor-I (IGF-I) concentrations showed comparable expression levels of hepatic growth hormone receptor (GHR). Suppressor of cytokine signaling 2 (SOCS2), could be responsible for additional inhibition of the GHR signal cascade. The aims were to monitor cows with high or low antepartal IGF-I concentrations (IGF-I(high) or IGF-I(low)), evaluate the interrelationships of endocrine endpoints, and measure hepatic SOCS2 expression. Dairy cows (n = 20) were selected (240 to 254 days after artificial insemination (AI)). Blood samples were drawn daily (day -17 until calving) and IGF-I, GH, insulin, thyroid hormones, estradiol, and progesterone concentrations were measured. Liver biopsies were taken (day 264 +/- 1 after AI and postpartum) to measure mRNA expression (IGF-I, IGFBP-2, IGFBP-3, IGFBP-4, acid labile subunit (ALS), SOCS2, deiodinase1, GHR1A). IGF-I concentrations in the two groups were different (p < 0.0001). However, GH concentrations and GHR1A mRNA expression were comparable (p > 0.05). Thyroxine levels and ALS expression were higher in the IGF-I(high) cows compared to IGF-I(low) cows. Estradiol concentration tended to be greater in the IGF-I(low) group (p = 0.06). It was hypothesized that low IGF-I levels are associated with enhanced SOCS2 expression although this could not be decisively confirmed by the present study.
Animals
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Cattle
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Estradiol/blood
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Female
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Growth Hormone/blood
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Insulin/blood
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Insulin-Like Growth Factor Binding Protein 2/analysis
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Insulin-Like Growth Factor Binding Protein 3/analysis
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Insulin-Like Growth Factor Binding Protein 4/analysis
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Insulin-Like Growth Factor I/*analysis/physiology
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Liver/chemistry
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Pregnancy/metabolism/physiology
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Pregnancy, Animal/*metabolism/physiology
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Progesterone/blood
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Suppressor of Cytokine Signaling Proteins/analysis
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Thyroid Hormones/blood
2.Serum insulin-like growth factor (IGF)-I and IGF-binding proteins in lung cancer patients.
Dae Yeol LEE ; Sun Jun KIM ; Yong Chul LEE
Journal of Korean Medical Science 1999;14(4):401-404
Many studies have shown that insulin-like growth factors (IGF-I & IGF-II) are implicated in the autocrine and paracrine growth of various tumors. Alterations in serum IGFs and IGF-binding proteins (IGFBPs) profiles have been reported in lung cancer. In this study, we measured serum levels of IGF-I and IGFBPs in 41 patients with lung cancer (small cell lung cancer, SCLC, 9; non-small cell lung cancer, NSCLC, 32) by radioimmunoassay and Western ligand blot (WLB). The serum IGF-I level in patients with lung cancer was significantly lower than in controls (207.9+/-62.6 vs 281.3+/-53.9 ng/mL, p<0.01). Patients with NSCLC showed significantly lower serum levels of IGF-I compared with SCLC patients (194.0+/-62.9 vs 258.4+/-27.8 ng/mL, p<0.01). Patients with squamous cell carcinoma tended to show lower serum levels of IGF-I than in those with adenocarcinoma (187.9+/-63.6 vs 215.9+/-59.5 ng/mL, p>0.05). The concentration of IGFBP-3 in lung cancer was 48% of that found in controls by WLB. The serum level of IGFBP-2 was markedly elevated in patients with lung cancer compared with controls (1303.7+/-618.0 vs 696.2+/-300.5, p<0.01). However, there was no significant difference between SCLC and NSCLC groups. This result showed that serum level of IGF-I/IGFBPs may be useful markers for diagnosing and identifying tumor types in lung cancer and further studies are needed.
Adenocarcinoma/diagnosis
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Adenocarcinoma/blood
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Adult
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Aged
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Blotting, Western
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Carcinoma, Small Cell/diagnosis
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Carcinoma, Small Cell/blood*
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Carcinoma, Squamous Cell/diagnosis
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Carcinoma, Squamous Cell/blood
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Female
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Human
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Insulin-Like Growth Factor Binding Protein 3/blood*
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Insulin-Like Growth Factor I/metabolism*
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Insulin-Like Growth Factor II/analysis
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Insulin-Like Growth Factor-Binding Protein 2/blood*
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Lung Neoplasms/diagnosis
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Lung Neoplasms/blood*
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Male
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Middle Age
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Radioimmunoassay
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Tumor Markers, Biological