CD44v6 was known as tumor marker for tumor progression and metastasis in various kinds of carcinomas. The CD44v6 monoclonal antibody was produced by cell cultures or mouse ascite fluids using CD44v6 hybridoma cells, and its immunogloburin G (IgG) was purified by Protein A column. Using immobilized ficin and cysteine, the antibody fragment Fab was produced and purified by Protein A. Four CD44v6 scFv molecules were produced from the recombinant DNA and phage antibody technology and prurified by His-tag affinity chromatography. In order to inspect the function and specificity of each antibody molecule, western-blotting and ELISA against CD44v5-6 recombinant proteins and irnmunodetection in human ovarian carcinomas were estabilished. The results showed that immunodiagnosis did not distinguish the types of antibody fragments, but western-blotting and ELISA results did show some difference of their specificities and biological properties. These studies will contribute as a model study for the immunodiagnosis and therapy using the IgG, Fab and scFv of CD44v6 antibody to obtain the early detection of tumor progression and metastasis using immunoscintigraphy.
Animals ; Bacteriophages ; Cell Culture Techniques ; Chromatography, Affinity ; Cysteine ; DNA, Recombinant ; Enzyme-Linked Immunosorbent Assay ; Ficain ; Humans ; Hybridomas ; Immunoglobulin Fragments* ; Immunoglobulin G ; Immunologic Tests ; Mice ; Neoplasm Metastasis ; Recombinant Proteins ; Sensitivity and Specificity ; Staphylococcal Protein A

Purpose: This study aimed to evaluate the effectiveness of an infection control education (ICE) program based on digital story-telling for new graduate nurses. Methods: From June 2 to July 30, 2022, a total of 58 new graduate nurses (30 and 28 nurses in the experimental and control group, respectively) at a tertiary general hospital in D city were enrolled. The effects were evaluated using a nonequivalent control group, non-synchronized design. The effects of the program were evaluated in terms of awareness of standard precautions, performance of standard precautions, and grit. Results: Statistically significant differences between the two groups were found in the awareness of standard precautions (t=-2.18, p=.034) and the performance of standard precautions (t=-3.27, p=.002). Grit was also significantly higher in the experimental group (t=-4.17, p<.001). Conclusion The development and application of the ICE program effectively improved the awareness of standard precautions, the performance of standard precautions, and grit among new nurses.

Sunitinib is a multi-target tyrosine kinase inhibitor used to treat gastrointestinal stromal tumors, renal cell carcinoma, and pancreatic neuroendocrine tumors. The most common adverse reactions are known to be nausea, fatigue, diarrhea, stomatitis, esophagitis, hypertension, skin toxicity (hand-foot syndrome), hypothyroidism, and reduction in the cardiac output of the left ventricle. Herein, we report the case of a 57 year-old female who visited our hospital complaining of epigastric pain. She had been taking sunitinib at 25 mg/day to treat a metastatic pancreatic neuroendocrine tumor. Upon computed tomography performed on admission, we observed that fluid had collected around the pancreas. Laboratory analysis revealed hypertriglyceridemia (triglycerides 993 mg/dL). Tyrosine kinase inhibitors are known to have limited effects on lipid metabolism. In this case, we suggest that hyperglycemia seems to have had a limited effect on lipid levels. We are rather of the view that hyperglycemia, a history of distal pancreatectomy, and hypothyrodisim, indirectly caused the observed hypertriglyceridemia.
Carcinoma, Renal Cell ; Cardiac Output ; Diarrhea ; Esophagitis ; Fatigue ; Female ; Gastrointestinal Stromal Tumors ; Heart Ventricles ; Humans ; Hyperglycemia ; Hypertension ; Hypertriglyceridemia* ; Hypothyroidism ; Lipid Metabolism ; Nausea ; Neuroendocrine Tumors* ; Pancreas ; Pancreatectomy ; Protein-Tyrosine Kinases ; Skin ; Stomatitis

PURPOSE: The purpose of this study was to survey the health behaviors related to reproductive health, reproductive health history, and sexual behaviors of college students in local areas. METHOD: With a survey design, 792 college students from C and D areas were recruited to assess health behaviors such as smoking and alcohol drinking, as well as history of reproductive health and sexual behaviors. The data were collected by a self-administered questionnaire from November to December, 2007. RESULT: Mean age of the students was 20.8(SD=1.96) and gender distribution was 29.2% for male and 70.8% for female. Unhealthy behaviors were alcohol drinking, smoking, excessive weight loss, and irregular exercise; unsafe sex practices were experience of sexual intercourse with multiple sex partners and history of sexually transmitted disease (STD). Male students had more alcohol drinking (p=.04), smoking(p<.001), excessive weight loss(p=.01), experience of sexual intercourse with multiple sex partners(p<.001), history of STD(p=.025) compared to females. On the other hands, female students participated in regular exercise less than males(p<.001). CONCLUSION: College students seem to perform unhealthy behaviors related to reproductive health and less effective way of safe sex practices. Therefore, a planned education program for reproductive health promotion operated by college level is needed to assess and improve the level of reproductive health in college students.
Alcohol Drinking ; Coitus ; Female ; Hand ; Health Behavior ; Humans ; Male ; Surveys and Questionnaires ; Reproductive Health ; Safe Sex ; Sexual Behavior ; Sexually Transmitted Diseases ; Smoke ; Smoking ; Unsafe Sex ; Weight Loss

PURPOSE: The purpose of this study was to investigate the occurrence of first onset of constipation during the rehabilitation stage and risk factors for constipation in patients with cerebral vascular disease (CVD). METHODS: Participants for this retrospective study were 214 CVD patients admitted to rehabilitation units. First onset of constipation and factors influencing constipation such as dependency level were recorded for 14 days during the rehabilitation stage. Survival analysis with Cox proportional hazard model and descriptive statistics were conducted. RESULTS: Age, patients'severity, types of diet, and dependency level were different between constipation and non-constipation groups. In survival analysis, 99% of participants developed constipation within 14 days after admission to rehabilitation units. Median constipation occurrence time was 7.4 days. Patients who were immobile and had tube feedings were more likely to develop constipation 4.07 times (95% CI: 1.018~16.301, p=.047) and 2.09 times (95% CI: 1.001~4.377, p=.050) respectively compared to patients who were independent and had a regular diet, respectively. CONCLUSION: Most CVD patients experienced constipation within 2 weeks after entering the rehabilitation stage. Constipation was linked to types of diet and dependency level. These factors associated with constipation should be considered when caring for patients in rehabilitation.
Constipation* ; Diet ; Humans ; Proportional Hazards Models ; Rehabilitation* ; Retrospective Studies ; Risk Factors ; Stroke ; Survival Analysis ; Vascular Diseases*

Cushing disease is caused by excessive adrenocorticotropic hormone (ACTH) production by the pituitary adenoma. Transsphenoidal surgery is its first-line treatment. The incidence of Cushing disease in children and adolescents is so rare that long-term prognoses have yet to be made in most cases. We followed-up on a 16-year-old male Cushing disease patient who presented with rapid weight gain and growth retardation. The laboratory findings showed increased 24-hour urine free cortisol and lack of overnight cortisol suppression by low-dose dexamethasone test. The serum cortisol and 24-hour urine free cortisol, by high-dose dexamethasone test, also showed a lack of suppression, and a bilateral inferior petrosal sinus sampling suggested lateralization of ACTH secretion from the right-side pituitary gland. However, after a right hemihypophysectomy by the transsphenoidal approach, the 24-hour urine free cortisol levels were persistently high. Thus the patient underwent a total hypophysectomy, since which time he has been treated with hydrocortisone, levothyroxine, recombinant human growth hormone, and testosterone enanthate. Intravenous bisphosphonate for osteoporosis had been administered for three years. At his current age of 26 years, his final height had attained the target level range; his bone mineral density was normal, and his pubic hair was Tanner stage 4. This report describes the long-term treatment course of a Cushing disease patient according to growth profile, pubertal status, and responses to hormone replacement therapy. The clinical results serve to emphasize the importance of growth optimization, puberty, and bone health in the treatment management of Cushing disease patients who have undergone transsphenoidal surgery.
Adolescent ; Adrenocorticotropic Hormone ; Bone Density ; Child ; Dexamethasone ; Follow-Up Studies* ; Hair ; Hormone Replacement Therapy ; Human Growth Hormone ; Humans ; Hydrocortisone ; Hypophysectomy ; Hypopituitarism ; Incidence ; Male ; Osteoporosis ; Petrosal Sinus Sampling ; Pituitary ACTH Hypersecretion* ; Pituitary Gland ; Pituitary Neoplasms ; Prognosis ; Puberty ; Testosterone ; Thyroxine ; Weight Gain

PURPOSE: Noonan syndrome (NS) is characterized by short stature, heart anomalies, developmental delays, dysmorphic features, cryptorchidism, and coagulation defects. Several studies reported the short-term effects of recombinant human growth hormone (rhGH) treatment on the improvement of height. This study was performed to evaluate the long-term efficacy of rhGH in children with NS in Korea. METHODS: This study included 15 prepubertal NS children who received rhGH subcutaneously at a dose of 50-75 µg/kg/day for 6 days a week for at least >3 years. Preand posttreatment data, such as height, weight, bone age, insulin-like growth factor 1 (IGF-1), and IGF binding protein 3 (IGFBP-3) levels, were collected every 6 months. RESULTS: Chronologic age and bone age at the start of treatment were 7.97±1.81 and 5.09±2.12 years, respectively. Height standard deviation score (SDS) was increased from -2.64±0.64 to -1.54±1.24 years after 3 years (P<0.001). Serum IGF-1 SDS levels were elevated from -1.28±1.03 to -0.10±0.94 (P<0.001). Height SDS was more increased in subjects without PTPN11 mutations compared to those with mutations after 3 years (P=0.012). However, the other parameters, including bone age, IGF-1 SDS, and IGFBP-3 SDS, were not significantly different between patients with and without PTPN11 mutations. CONCLUSION: Although this study included a relatively small number of patients, long-term rhGH therapy in NS patients was safe and effective at improving height, growth velocity, and serum IGF-1 levels, in accordance with previous studies. However, the meticulous monitoring of potential adverse events is still needed because of high dose of rhGH and preexisting hyperactivity of RAS-MAPK pathway. Patients with PTPN11 mutations demonstrated a decreased response to rhGH therapy compared to those without mutations.
Child ; Cryptorchidism ; Growth Hormone ; Heart ; Human Growth Hormone* ; Humans* ; Insulin-Like Growth Factor Binding Protein 3 ; Insulin-Like Growth Factor I ; Korea ; Male ; Noonan Syndrome*

Objectives: Education in biomedical and health informatics is essential for managing complex healthcare systems, bridging the gap between healthcare and information technology, and adapting to the digital requirements of the healthcare industry. This review presents the current status of biomedical and health informatics education domestically and internationally and proposes recommendations for future development. Methods: We analyzed evidence from reports and papers to explore global trends and international and domestic examples of education. The challenges and future strategies in Korea were also discussed based on the experts’ opinions. Results: This review presents international recommendations for establishing education in biomedical and health informatics, as well as global examples at the undergraduate and graduate levels in medical and nursing education. It provides a thorough examination of the best practices, strategies, and competencies in informatics education. The review also assesses the current state of medical informatics and nursing informatics education in Korea. We highlight the challenges faced by academic institutions and conclude with a call to action for educators to enhance the preparation of professionals to effectively utilize technology in any healthcare setting. Conclusions To adapt to the digitalization of healthcare, systematic and continuous workforce development is essential. Future education should prioritize curriculum innovations and the establishment of integrated education programs, focusing not only on students but also on educators and all healthcare personnel in the field. Addressing these challenges requires collaboration among educational institutions, academic societies, government agencies, and international bodies dedicated to systematic and continuous workforce development.