1.The distribution of inositol 1,4,5-trisphosphate 3-kinase in rat cerebellum.
Hae Kyoung LEE ; Jae Pil KO ; Seung Jun HWANG ; Young Suk SUH
Korean Journal of Anatomy 1993;26(1):94-102
No abstract available.
Animals
;
Cerebellum*
;
Inositol 1,4,5-Trisphosphate*
;
Inositol*
;
Rats*
2.Distribution of inositol 1,4,5-trisphosphate 3-kinase in the cat brain by immunohistochemical study.
Dong Hou KIM ; Hyun KIM ; Seung Jun HWANG ; Hae Nam HONG ; Young Suk SUH
Korean Journal of Anatomy 1993;26(4):378-389
No abstract available.
Animals
;
Brain*
;
Cats*
;
Inositol 1,4,5-Trisphosphate*
;
Inositol*
3.The effect of Ni2+ on the intracellular Ca2+ increase of the mouse early 2-cell embryos.
Sook Young YOON ; Eun Mi LEE ; In Ha BAE
Korean Journal of Fertility and Sterility 2003;30(4):269-280
OBJECTIVE: We reported the overcoming effect of Ni2+ on the in vitro 2-cell block of mouse embryos. In this study, we aim to investigate whether Ni2+ should induce intracellular Ca2+ transient in the mouse embryos. MATERIALS AND METHODS: Embryos were collected at post hCG 32hr from the oviduct of the ICR mouse and cultured in M2 medium omitted phenol red. Intracellular Ca2+ was checked by using a confocal laser scanning microscope and fluo-3AM by using various intracellular Ca2+ antagonists. RESULTS: In 1mM Ni2+ treated medium which contained Ca2+(1.71mM), 75.7% of the embryos showed [Ca2+]i transient about 200 sec later. In the Ca2+-free medium, 69.8% of the embryos showed [Ca2+]i transient. In U73122, phospholipaseC(PLC) inhibitor (5uM, 10min) pretreated group, 33.3% of the embryos showed [Ca2+]i transient. Heparine, inositol 1,4,5-triphosphate receptor(IP3R) antagonist preinjected embryos showed no response with 1mM Ni2+. In danthrolene treatment, ryanodine receptor(RyR)-antagonist, 43% embryos showed [Ca2+]i transient but they showed delayed response about 340sec in the presence of Ca2+. CONCLUSIONS: Summing up the above results, Ni2+ seems to induce Ca2+-release from the Ca2+-store even in the Ca2+-free medium. IP3 receptors of the mouse 2-cell embryos might have an essential role for the intracellular Ca2+ increase by Ni2+.
Animals
;
Embryonic Structures*
;
Heparin
;
Inositol 1,4,5-Trisphosphate
;
Inositol 1,4,5-Trisphosphate Receptors
;
Mice*
;
Mice, Inbred ICR
;
Oviducts
;
Phenolsulfonphthalein
;
Ryanodine
4.Effects of the ITPR1 gene overexpression on Ca²⁺ concentration, lipid content and calcium transport-related genes in duck uterine epithelial cells.
Minfang YOU ; Yuanyu QIN ; Yiyu ZHANG ; Chaomei LIAO ; Guanghui TAN ; Jiezhang LI ; Wangui LI
Chinese Journal of Biotechnology 2021;37(7):2443-2452
Inositol 1,4,5-trisphosphate receptor 1 (ITPR1) is an important intracellular channel for releasing Ca²⁺. In order to investigate the effects of the ITPR1 overexpression on Ca²⁺ concentration and lipid content in duck uterine epithelial cells and its effects on calcium transport-related genes, the structural domain of ITPR1 gene of duck was cloned into an eukaryotic expression vector and transfected into duck uterine epithelial cells. The overexpression of the ITPR1 gene, the concentration of Ca²⁺, the lipid content, and the expression of other 6 calcium transport-related genes was determined. The results showed that the concentration of Ca²⁺ in uterine epithelial cells was significantly reduced after transfection (P<0.05), the triglyceride content was significantly increased (P<0.01), and the high-density lipoprotein content was significantly decreased (P<0.01). The correlation analysis results showed that the overexpression of the C-terminal half of the ITPR1 gene was significantly positively correlated with the total cholesterol content (P<0.01), which was significantly positively correlated with the low-density lipoprotein content (P<0.05). The overexpression of the N-terminal half of the ITPR1 gene was significantly positively correlated with the triglyceride content (P<0.01), which was significantly negatively correlated with the concentration of Ca²⁺ (P<0.05). RT-qPCR results of 6 calcium transport-related genes showed that the overexpression of the C-terminal half of the ITPR1 gene significantly inhibited the expression of the IP3R2, VDAC2 and CAV1 genes, and the overexpression of the N-terminal half of the ITPR1 gene significantly promoted the expression of the IP3R3 and CACNA2D1 genes. In conclusion, the ITPR1 gene overexpression can promote Ca²⁺ release in duck uterus epithelial cells, promote the synthesis of triglyceride, low-density lipoprotein and cholesterol, and inhibit the production of high-density lipoprotein, and the ITPR1 gene overexpression affected the expression of all 6 calcium transport-related genes.
Animals
;
Calcium/metabolism*
;
Ducks/genetics*
;
Epithelial Cells
;
Female
;
Inositol
;
Inositol 1,4,5-Trisphosphate Receptors
;
Lipids
;
Uterus
5.Caffeine and 2-Aminoethoxydiphenyl Borate (2-APB) Have Different Ability to Inhibit Intracellular Calcium Mobilization in Pancreatic Acinar Cell.
Kyung Jin CHOI ; Kab Sung KIM ; Se Hoon KIM ; Dong Kwan KIM ; Hyung Seo PARK
The Korean Journal of Physiology and Pharmacology 2010;14(2):105-111
Inositol 1,4,5-trisphosphate receptors (InsP3Rs) modulate Ca2+ release from intracellular Ca2+ store and are extensively expressed in the membrane of endoplasmic/sarcoplasmic reticulum and Golgi. Although caffeine and 2-aminoethoxydiphenyl borate (2-APB) have been widely used to block InsP3Rs, the use of these is limited due to their multiple actions. In the present study, we examined and compared the ability of caffeine and 2-APB as a blocker of Ca2+ release from intracellular Ca2+ stores and Ca2+ entry through store-operated Ca2+ (SOC) channel in the mouse pancreatic acinar cell. Caffeine did not block the Ca2+ entry, but significantly inhibited carbamylcholine (CCh)-induced Ca2+ release. In contrast, 2-APB did not block CCh-induced Ca2+ release, but remarkably blocked SOC-mediated Ca2+ entry at lower concentrations. In permeabilized acinar cell, caffeine had an inhibitory effect on InsP3-induced Ca2+ release, but 2-APB at lower concentration, which effectively blocked Ca2+ entry, had no inhibitory action. At higher concentrations, 2-APB has multiple paradoxical effects including inhibition of InsP3-induced Ca2+ release and direct stimulation of Ca2+ release. Based on the results, we concluded that caffeine is useful as an inhibitor of InsP3R, and 2-APB at lower concentration is considered a blocker of Ca2+ entry through SOC channels in the pancreatic acinar cell.
Acinar Cells
;
Animals
;
Boron Compounds
;
Caffeine
;
Calcium
;
Carbachol
;
Inositol 1,4,5-Trisphosphate Receptors
;
Membranes
;
Mice
;
Reticulum
6.Pharmacological evidence that cromakalim inhibits Ca2+ release from intracellular stores in porcine coronary artery.
Byung Yong RHIM ; Sun Hwa HONG ; Chi Dae KIM ; Won Suk LEE ; Ki Whan HONG
The Korean Journal of Physiology and Pharmacology 1997;1(1):27-34
In the present study, it was aimed to further identify the intracellular action mechanism of cromakalim and levcromakaliin in the porcine coronary artery. In intact porcine coronary arterial strips loaded with fura-2/AM, acetylcholine caused an increase in intracellular free Ca2+ ((Ca2+)-i) in association with a contraction in a concentration-dependent manner. Cromakalim (1 micrometer) caused a reduction in acetylcholine-induced increased (Ca2+)-i not only in the normal physiological salt solution (PSS) but also in Ca2+ -free PSS (containing 1mM EGTA). In the skinned strips prepared by exposure of tissue to 20 micrometer beta-escin, inositol 1,4,5-trisphosphate (IP-3) evoked an increase in (Ca2+)-i but it was without effect on the intact strips. The IP-3-induced increase in (Ca2+)-i was inhibited by cromakalim by 78% and levcromakalim by 59% (1 micrometer, each). Pretreatment with glibenclamide (a blocker of ATP-sensitive K+ channels, 10 micrometer and apamin (a blocker of small conductance Ca2+/-activated K+ channels, 1 micrometer strongly blocked the effect of cromakalim and levcromakalim. However, charybdotoxin (a blocker of large conductance Ca2+ -activated K+ channels, 1-micrometer) was without effect. In addition, cromakalim inhibited the GTP-gamma-S (100 micrometer, nonhydrolysable analogue of GTP)-induced increase in (Ca2+)-i. Based on these results, it is suggested that cromakalim and levcromakalim exert a potent vasorelaxation, in part, by acting on the K+ channels of the intracellular sites (e.g., sarcoplasmic reticulum membrane), thereby, resulting in decrease in release of Ca2+ from the intracellular storage site.
Acetylcholine
;
Apamin
;
Charybdotoxin
;
Coronary Vessels*
;
Cromakalim*
;
Escin
;
Glyburide
;
Inositol 1,4,5-Trisphosphate
;
Sarcoplasmic Reticulum
;
Skin
;
Vasodilation
7.Diagnosis of a patient with Spinocerebellar ataxia type 29 due to a novel variant of ITPR1 gene.
Ya Nan ZHI ; Jiao LIU ; Cheng ZHEN ; Juan LI ; Fangna WANG ; Yan LUO ; Pingping ZHANG ; Mingming ZHANG ; Yali LI
Chinese Journal of Medical Genetics 2023;40(1):76-80
OBJECTIVE:
To explore the clinical and genetic characteristics of a child with spinocerebellar ataxia type 29 (SCA29) due to novel variant of the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) gene.
METHODS:
The child was subjected high-throughput sequencing, and candidate variant was verified by Sanger sequencing of his family members.
RESULTS:
The child was found to harbor a c.800C>T (p.T267M) variant of the ITPR1 gene, which was not found in his parents and their fetus. The variant has occurred in a hotspot of the ITPR1 gene variants and was unreported before in China. Based on his clinical and genetic characteristics, the child was diagnosed with SCA29.
CONCLUSION
The novel heterozygous c.800C>T (p.T267M) of the ITPR1 gene probably underlay the SCA29 in this child.
Child
;
Humans
;
Family
;
Inositol 1,4,5-Trisphosphate Receptors/genetics*
;
Mutation
;
Spinocerebellar Ataxias/genetics*
;
Spinocerebellar Degenerations
8.The Transcription Factor Mist1 Regulates the Cellular Polarity in Mouse Pancreatic Acinar Cells.
Yu Mi YANG ; Syng Ill LEE ; Dong Min SHIN
International Journal of Oral Biology 2012;37(1):37-41
Pancreatic acinar cells exhibit a polarity that is characterized by the localization of secretory granules at the apical membrane. However, the factors that regulate cellular polarity in these cells are not well understood. In this study, we investigated the effect of Mist1, a basic helix-loop-helix transcription factor, on the cellular architecture of pancreatic acinar cells. Mist1-null mice displayed secretory granules that were diffuse throughout the pancreatic acinar cells, from the apical to basolateral membranes, whereas Mist1 heterozygote mice showed apical localization of secretory granules. Deletion of the Mist1 gene decreased the expression of type 3 inositol 1,4,5-triphosphate receptors (IP3R) but did not affect apical localization and expression of IP3R2. Mist1-null mice also displayed an increase in luminal areas and an increase in the expression of zymogen granules in pancreatic acinar cells. These results suggest that Mist1 plays a critical role in polar localization of cellular organelles and in maintaining cellular architecture in mouse pancreatic acinar cells.
Acinar Cells
;
Animals
;
Cell Polarity
;
Heterozygote
;
Inositol 1,4,5-Trisphosphate Receptors
;
Membranes
;
Mice
;
Organelles
;
Phenobarbital
;
Secretory Vesicles
;
Transcription Factors
9.House Dust Mite Extract Induces PLC/IP3-dependent Ca2+ Signaling and IL-8 Expression in Human Gingival Epithelial Cells.
Ga Yeon SON ; Aran SON ; Wonse PARK ; Dong Min SHIN
International Journal of Oral Biology 2015;40(1):11-17
The gingival epithelium of the oral cavity is constantly exposed to exogenous stimuli such as bacterial toxins, allergens, and thermal changes. These exogenous stimuli are resisted by innate host defense in gingival epithelial cells. However, it is unclear exactly how the exogenous stimuli affect detrimentally on the human gingival epithelial cells. Here, we investigated whether the allergen, such as house dust mite (HDM) extract, is linked to Ca2+ signaling and proinflammatory cytokine expression in primary cultured human gingival epithelial cells. HDM extract induced an increase in intracellular Ca2+ concentration ([Ca2+]i) in a dose-dependent manner. Extracellular Ca2+ depletion did not affected on the HDM extract-induced increase in [Ca2+]i. The HDM extract-induced increase in [Ca2+]i was abolished by the treatment with U73122 and 2-APB, which are inhibitors of phospholipase C (PLC) and inositol 1,4,5-trisphosphate (IP3) receptor. Moreover, HDM extract induced the mRNA expression of pro-inflammatory cytokine, interleukin (IL)-8. These results suggest that HDM extract triggers PLC/IP3-dependent Ca2+ signaling and IL-8 mRNA expression in primary cultured human gingival epithelial cells.
Allergens
;
Bacterial Toxins
;
Epithelial Cells*
;
Epithelium
;
Humans
;
Inositol 1,4,5-Trisphosphate
;
Interleukin-8*
;
Interleukins
;
Mouth
;
Pyroglyphidae*
;
RNA, Messenger
;
Type C Phospholipases
10.Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive Calcium Channel.
Min Gu PARK ; Heeyeong JANG ; Sang Hoon LEE ; C Justin LEE
Experimental Neurobiology 2017;26(2):71-81
Radial glial cells (RGCs) which function as neural stem cells are known to be non-excitable and their proliferation depends on the intracellular calcium (Ca²⁺) level. It has been well established that Inositol 1,4,5-trisphosphate (IP3)-mediated Ca²⁺ release and Ca²⁺ entry through various Ca²⁺ channels are involved in the proliferation of RGCs. Furthermore, RGCs line the ventricular wall and are exposed to a shear stress due to a physical contact with the cerebrospinal fluid (CSF). However, little is known about how the Ca²⁺ entry through mechanosensitive ion channels affects the proliferation of RGCs. Hence, we hypothesized that shear stress due to a flow of CSF boosts the proliferative potential of RGCs possibly via an activation of mechanosensitive Ca²⁺ channel during the embryonic brain development. Here, we developed a new microfluidic two-dimensional culture system to establish a link between the flow shear stress and the proliferative activity of cultured RGCs. Using this microfluidic device, we successfully visualized the artificial CSF and RGCs in direct contact and found a significant enhancement of proliferative capacity of RGCs in response to increased shear stress. To determine if there are any mechanosensitive ion channels involved, a mechanical stimulation by poking was given to individual RGCs. We found that a poking on radial glial cell induced an increase in intracellular Ca²⁺ level, which disappeared under the extracellular Ca²⁺-free condition. Our results suggest that the shear stress by CSF flow possibly activates mechanosensitive Ca²⁺ channels, which gives rise to a Ca²⁺ entry which enhances the proliferative capacity of RGCs.
Brain
;
Calcium Channels*
;
Calcium*
;
Cerebrospinal Fluid
;
Ependymoglial Cells*
;
Inositol 1,4,5-Trisphosphate
;
Ion Channels
;
Lab-On-A-Chip Devices
;
Microfluidics
;
Neural Stem Cells