PURPOSE: To determine CD133+ cells defined as cancer stem cells (CSCs) in colon cancer, we examined whether CD133+ clones in HCT116 demonstrate known features of CSCs like sphere-forming ability, chemodrug-resistance, and metastatic potential. METHODS: Magnetic cell isolation and cell separation demonstrated that <1% of HCT116 cells expressed CD133, with the remaining cells being CD133- clones. In colon cancer cells, radioresistance is also considered a CSC characteristic. We performed clonogenic assay using 0.4 Gy γ-irradiation. RESULTS: Interestingly, there were no differences between HCT116 parental and HCT116 CD133+ clones when the cells comprised 0.5% of the total cells, and CD133- clone demonstrated radiosensitive changes compared with parental and CD133+ clones. Comparing gene expression profiles between sphere-forming and nonforming culture conditions of HCT116 subclones by whole RNA sequencing failed to obtain specific genes expressed in CD133+ clones. CONCLUSION: Despite no differences of gene expression profiles in monolayer attached culture conditions of each clone, sphere-forming conditions of whole HCT116 subclones, parental, CD133+, and CD133- increased 1,761 coding genes and downregulated 1,384 genes related to CSCs self-renewal and survival. Thus, spheroid cultures of HCT116 cells could be useful to expand colorectal CSCs rather than clonal expansion depending on CD133 expressions.
Cell Separation ; Clinical Coding ; Clone Cells* ; Colonic Neoplasms ; HCT116 Cells ; Humans ; Neoplastic Stem Cells ; Parents ; RNA* ; Sequence Analysis, RNA* ; Transcriptome

The present study investigated caffeinated beverage consumption and screen time in the association with excessive daytime sleepiness (EDS) and sleep duration. We conducted a cross-sectional study including 249 Korean male high school students. These participants responded to a questionnaire inquiring the information on lifestyle factors, consumption of caffeinated beverages, time spent for screen media, and sleep duration as well as to the Epworth Sleepiness Scale (ESS) questionnaire. EDS was defined as ESS scores of 9 or greater. Students with EDS consumed greater amount of chocolate/cocoa drinks and spent longer time for a TV and a mobile phone than those without EDS (p < 0.05). In addition, students with short sleep (≤ 6 hours) consumed greater amount of coffee than others whereas students with long sleep (> 8 hours) consumed greater amount of chocolate/cocoa drinks than others (p < 0.05). Screen time did not differ according to the categories of sleep duration. Although these findings do not support causal relationships, they suggest that screen time is associated with EDS, but not with sleep duration, and that consumption of certain types of caffeinated beverages is associated with EDS and sleep duration. Adolescents may need to reduce screen time and caffeine consumption to improve sleep quality and avoid daytime sleepiness.
Adolescent ; Beverages* ; Caffeine ; Cell Phones ; Coffee ; Cross-Sectional Studies ; Humans ; Life Style ; Male

Functional interpretation of noncoding genetic variants associated with complex human diseases and traits remains a challenge. In an effort to enhance our understanding of common germline variants associated with lung cancer, we categorize regulatory elements based on eight major cell types of human lung tissue. Our results show that 21.68% of lung cancer‒associated risk variants are linked to noncoding regulatory elements, nearly half of which are cell type‒specific. Integrative analysis of high-resolution long-range chromatin interactome maps and single-cell RNA-sequencing data of lung tumors uncovers number of putative target genes of these variants and functionally relevant cell types, which display a potential biological link to cancer susceptibility. The present study greatly expands the scope of functional annotation of lung cancer‒associated genetic risk factors and dictates probable cell types involved in lung carcinogenesis.

Functional interpretation of noncoding genetic variants associated with complex human diseases and traits remains a challenge. In an effort to enhance our understanding of common germline variants associated with lung cancer, we categorize regulatory elements based on eight major cell types of human lung tissue. Our results show that 21.68% of lung cancer‒associated risk variants are linked to noncoding regulatory elements, nearly half of which are cell type‒specific. Integrative analysis of high-resolution long-range chromatin interactome maps and single-cell RNA-sequencing data of lung tumors uncovers number of putative target genes of these variants and functionally relevant cell types, which display a potential biological link to cancer susceptibility. The present study greatly expands the scope of functional annotation of lung cancer‒associated genetic risk factors and dictates probable cell types involved in lung carcinogenesis.

Langerhans cell histiocytosis (LCH) refers a group of disorders of the reticuloendothelial system characterized by a proliferation of histiocytes, which includes eosinophilic granuloma, Lettere-Siwe diseases, and Hand-Schuler Christian disease. The clinical presentation of LCH depends on the site of involvement. It can range from multifocal lesions to a solitary lesion. Tissues characteristically involved in LCH are bone, skin, lung, liver, spleen, bone marrow, lymph nodes and the hypothalamic-pituitary region, although the involvement of other organs such as the bowel can occur. We experienced a case of external auditory canal stenosis in LCH of multiple organ involvement in a 28-year-old male, and report it with a review of the relevant literature.
Adult ; Bone Marrow ; Constriction, Pathologic ; Ear Canal ; Eosinophilic Granuloma ; Histiocytes ; Histiocytosis, Langerhans-Cell ; Humans ; Liver ; Lung ; Lymph Nodes ; Male ; Mononuclear Phagocyte System ; Skin ; Spleen ; Temporal Bone

Langerhans cell histiocytosis (LCH) refers a group of disorders of the reticuloendothelial system characterized by a proliferation of histiocytes, which includes eosinophilic granuloma, Lettere-Siwe diseases, and Hand-Schuler Christian disease. The clinical presentation of LCH depends on the site of involvement. It can range from multifocal lesions to a solitary lesion. Tissues characteristically involved in LCH are bone, skin, lung, liver, spleen, bone marrow, lymph nodes and the hypothalamic-pituitary region, although the involvement of other organs such as the bowel can occur. We experienced a case of external auditory canal stenosis in LCH of multiple organ involvement in a 28-year-old male, and report it with a review of the relevant literature.
Adult ; Bone Marrow ; Constriction, Pathologic ; Ear Canal ; Eosinophilic Granuloma ; Histiocytes ; Histiocytosis, Langerhans-Cell ; Humans ; Liver ; Lung ; Lymph Nodes ; Male ; Mononuclear Phagocyte System ; Skin ; Spleen ; Temporal Bone

No abstract available.
Humans

Data are limited on the association between dietary patterns and screen time among Korean adolescents. The present study identified dietary patterns of 691 adolescents, aged 13-18 years, who had participated in the Third Korean National Health and Nutrition Examination Survey (KNHANES III) and analyzed their associations with screen time. Screen time was defined as the time spent watching TV, using a computer, or playing video games was calculated as a sum of all these times. Dietary patterns and their factor scores were derived from a food frequency questionnaire using the factor analysis method. To analyze the association between dietary patterns and screen time, we conducted multiple linear regression analysis. We also performed multiple logistic regression analysis to estimate odds ratios (OR) of excessive screen time (2 hours or longer per day) and 95% confidence intervals (CI). We identified 2 dietary patterns labeled "the Korean healthy dietary pattern" and "the Western diet and fast foods pattern". The former included mixed grains, legumes, potatoes, red meat, eggs, fish, dairy products, fruits, vegetables, seaweeds, and mushrooms, whereas the latter included noodles, bread, red meat, poultry, fast foods, snack, and soft drinks. After controlling for potential confounding factors, factor scores for the Korean healthy dietary pattern were inversely associated (P-value for trend < 0.01) and those for the Western diet and fast foods pattern were positively associated with the screen time (P-value for trend < 0.01). Adolescents in the top tertile of the scores for the Korean healthy dietary pattern had a multivariable-adjusted OR [95% CI] of 0.44 (0.25-0.75) for excessive screen time compared with those in the lowest tertile. On the basis of these findings, adolescents who have excessive screen time may need to be encouraged to consume a more healthy diet.
Adolescent ; Agaricales ; Aged ; Bread ; Carbonated Beverages ; Edible Grain ; Dairy Products ; Diet ; Eggs ; Fabaceae ; Fast Foods ; Fruit ; Humans ; Linear Models ; Logistic Models ; Meat ; Nutrition Surveys ; Odds Ratio ; Ovum ; Poultry ; Surveys and Questionnaires ; Snacks ; Solanum tuberosum ; Vegetables ; Video Games

There has been no genome-wide association study (GWAS) for macronutrient intake as a quantitative trait. To explore genetic loci associated with total calorie and macronutrient intake, genome-wide association data of autosomal single nucleotide polymorphisms (SNPs) from Korean adults were analyzed. We conducted a GWAS in 3,690 men and women aged 40 to 60 years from an urban population-based cohort. At the baseline examination (June 18, 2001 through January 29, 2003), DNA samples of the study subjects were collected and analyzed for genotyping. The information of average daily consumption of total calorie, carbohydrate, protein, and fat was obtained from a semi-quantitative food frequency questionnaire and transformed by natural logarithm for analyses after adjustment of calorie intake. Using multivariate linear regression analysis adjusted for age, sex, and height, we tested for 352,021 SNPs and found weak associations, which do not reach genome-wide association significance, with calorie and macronutrient intake. However, a number of SNPs were found to have potential associations with macronutrient intake; in particular, signals in SORBS1 and those in PRKCB1 were likely associated with carbohydrate and fat intake, respectively. We observed an inverse association between the minor allele of the SNPs in these genes and the amount of consumption of carbohydrate or fat. Our GWAS identified loci and minor alleles weakly associated with macronutrient intake. Because SORBS1 and PRKCB1 are reportedly associated with the metabolism of glucose and lipid as well as with obesity-related diseases, further investigations on biological and functional roles of polymorphism of these genes in the relation to macronutrient intake are warranted.
Adult ; Aged ; Alleles ; Cohort Studies ; DNA ; Female ; Genetic Loci ; Genome-Wide Association Study ; Glucose ; Humans ; Linear Models ; Lipid A ; Male ; Polymorphism, Single Nucleotide ; Surveys and Questionnaires

Myeloid sarcoma is a rare solid, extramedullary tumor composed of immature granulocytes, occurring in a granulocytic leukemia. In rare cases, they can represent the initial manifestation of a relapsed form in patients with acute myelogenous leukemia in remission status. There have been only a few reports of myeloid sarcoma involving the temporal bone. We report a case of facial nerve paralysis caused by temporal bone myeloid sarcoma as the presenting symptom of leukemic relapse in a 27-year-old female, who was affected by acute myelogenous leukemia.
Adult ; Facial Nerve ; Female ; Granulocytes ; Humans ; Leukemia, Myeloid ; Leukemia, Myeloid, Acute ; Paralysis ; Recurrence ; Sarcoma, Myeloid ; Temporal Bone