OBJECTIVETo explore a simple and reliable method for intraperitoneal injection through a paravertebral approach in rabbits.

METHODSSixty New Zealand rabbits were randomized into conventional group and modified groups to receive intraperitoneal injections through conventional and paravertebral approaches, respectively. In the conventional group, the injection site was on the abdominal wall 3~4 cm lateral from the umbilicus bilaterally, while that in the modified group was located dorsally at L5/L6 level 3-4 cm lateral from the midline. Abdominal CT scan was performed in the post-injection rabbits, which were sacrificed after 24 h for abdominal dissection.

RESULTSSuccess with a single puncture was achieved in 13 out of the 20 rabbits in the conventional group, and the rest required at least two punctures, with a mean rank sum of 23.50. With the modified approach, a single attempt was successful in all the 40 rabbits, with a mean rank sum of 34.0, showing a significant difference between the two groups (P<0.01). The success rates of a single injection differed significantly between the two groups (P<0.01). CT scan and abdominal dissection showed that the injection site with the modified approach was far away from the vital organs and large vessels with less peritoneal hyperemia and exudation.

CONCLUSIONParavertebral intraperitoneal paracentesis is a convenient and reliable method for intraperitoneal injection in rabbits.

Animals ; Injections, Intraperitoneal ; methods ; Rabbits

Humans ; Injections ; Laryngoplasty ; methods ; trends ; Vocal Cords

Desensitization, Immunologic ; methods ; Humans ; Injections, Subcutaneous

Vaccination is the most efficient method for infectious disease prevention. Parenteral injections such as intramuscular, intradermal, and subcutaneous injections have several advantages in vaccine delivery, but there are many drawbacks. Thus, the development of a new vaccine delivery system has long been required. Recently, microneedles have been attracting attention as new vaccination tools. Microneedle is a highly effective transdermal vaccine delivery method due to its mechanism of action, painlessness, and ease of use. Here, we summarized the characteristics of microneedles and the possibilities as a new vaccine delivery route.
Communicable Diseases ; Injections, Subcutaneous ; Methods* ; Vaccination ; Vaccines*

A liquid jet injector employs compressed gas or spring to produce a high-velocity stream to deliver liquid drug into human body through skin. There are many clinical jet injection products available, none of which is domestic. A new liquid jet injector is designed based on a comprehensive analysis of the current products. The injector consists of an ejector, trigger and a re-positioning mechanism. The jets characteristics of sample injector are tested, and the results show that the maximum exit pressure is above 15 MPa, a threshold value for penetrating into the skin.
Equipment Design ; Humans ; Injections, Intradermal ; instrumentation ; methods ; Injections, Jet ; instrumentation ; methods

In this article, based on the relative references in the latest 8 years, we introduce briefly the origin, preparation and application of various injectable and absorbable gelling scaffold materials, and outline their advantages and disadvantages as localized gelling scaffold systems.
Absorbable Implants ; Biocompatible Materials ; Gels ; chemistry ; Injections ; Tissue Engineering ; methods

The analysis of sperm morphology can be used to evaluate sperm fertilizing ability and spontaneous conception status, and especially the overall analysis of the sperm head, neck and tail, along with the patient's living habits, occupation and clinical manifestations, may contribute to the primary diagnosis of the patients potentia generandi. It can also be employed to assess the effects of the treatment of semen samples. Although oocyte fertilization can be achieved by the technologies of intracytoplasmic sperm injection (ICSI), motile sperm organelle morphology examination (MSOME) and intracytoplasmic morphologically selected sperm injection (IMSI) regardless of sperm morphology and / or motility, which may somewhat weaken the clinical application of sperm morphology analysis, the standardized procedure and the practice of quality control for the analysis of sperm morphology can significantly improve the accuracy of its results and largely promote its clinical application. Therefore, it is of positive necessity as well as clinical application value to perform sperm morphology analysis in andrology laboratories, reproductive centers, sperm banks and the department of laboratory medicine.
Humans ; Male ; Semen Analysis ; Sperm Injections, Intracytoplasmic ; methods ; Sperm Motility

OBJECTIVETo explore the effects of debridement combined with vacuum sealing drainage (VSD) technique in treatment of high-pressure paint injection injuries of hand.

METHODSFrom April 2005 to August 2010,14 patients with high-pressure paint injection injuries of hand were treated with debridement and VSD technique within 6 hours after injury. All the patients were male,ranging in age from 23 to 47 years with an average of 36.5 years. All injuries occurred left hand,thumb injured in 5 cases,index finger in 3 cases, middle finger in 2 cases and palm in 4 cases. Injured hands swelled obviously with poor blood circulation. When the wounds were covered with fresh granulation tissue without inflammatory effusion after operation of 3-4 times, the skingrafting (9 cases) or transfer flap (5 cases) were done on the wounds.

RESULTSAll the patients were followed up from 8 to 16 months with an average of 12 months. All the wounds obtained good healing. Therapeutic effects were estimated according to TAM criteria, 7 cases were excellent,6 good and 1 fair.

CONCLUSIONIn high-pressure paint injection injuries of hands,debridement combined with VSD technique can avoid wound infection,promote the growth of granulation tissue. It is beneficial to wound healing.

Adult ; Debridement ; methods ; Drainage ; methods ; Hand Injuries ; surgery ; Humans ; Injections ; Male ; Middle Aged ; Paint

Animals ; Endothelium, Vascular ; injuries ; Female ; Hemodynamics ; Infusions, Intravenous ; methods ; Injections ; methods ; Male ; Rabbits ; Syringes

Sperm selection plays an important role in assisted reproductive technology. In recent years, sperm evaluation is not limited to the assessment of sperm motility and morphology, but involves more other sperm characteristics such as sperm ultrastructure, DNA integrity, apoptosis and membrane. Assessment based on these characteristics is becoming the aim of sperm selection. This article gives an overview on several newly developed techniques for sperm selection according to different technical principles, such as electrophoretic separation, zeta potential, HA binding, Annexin V binding, intracytoplasmic morphologically selected sperm injection (IMSI) and microfluidic sperm sorter, which have all been applied to IVF or ICSI with the exception of microfluidic sperm sorter. It also introduces the advantages, disadvantages and application effects of these techniques.
Cell Separation ; Fertilization in Vitro ; methods ; Humans ; Male ; Reproductive Techniques, Assisted ; Semen Analysis ; Sperm Injections, Intracytoplasmic ; methods