1.Epidemiology of 1968 flu.
Fan YUAN ; Yu LAN ; Jun-Feng GUO ; Xin-Wan LI ; Min-Ju TAN ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():33-35
2.Epidemiology of 1957 flu.
Hong-Tao SUI ; Li-Mei YANG ; Wei WANG ; Li XIN ; Li-Bo DONG ; Wei-Juan HUANG ; He-Jiang WEI ; Cui-Ling XU ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():27-32
3.Review on the etiological property of 1957 Asian flu virus (H2N2).
Ning DU ; Xiao-Xing YANG ; Lei YANG ; Yu-Hong ZENG ; Shu-Mei ZOU ; Hong BO ; Yuan-Ji GUO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():12-16
4.Establishment of mammalian cell lines for constitutive expression of influenza virus matrix protein 2.
Ai-Jun CHEN ; Jian-Qiang GUO ; Li-Hong YAO ; Ji-Yong YIN ; Zhi-Qing ZHANG
Chinese Journal of Virology 2013;29(1):7-11
To establish a mammalian cell line for stable expression of the matrix protein 2 (M2) of influenza virus type A. M2 gene was amplified by PCR from the influenza virus strain A/PR/8/34. The PCR product was cloned into eukaryotic expression vector pcDNA5/FRT. After identification with restriction enzyme digestion, the plasmid was co-transfected with plasmid pOG44 which expressed Flp in Flp-In-CHO cells. The target gene was integrated into chromosome of CHO cells by homologous recombination in vivo. Recombinant CHO-M2 cell lines were selected for hygromycin B resistance. A total of 15 recombinant cell strains with high expression of M2 protein were screened by hygromycin, and the expression of M2 protein was determined by IFA and Western blot. After subculturing for 10 passages, the presence of M2 gene in the CHO-M2 cells was confirmed by PCR, and the expression of M2 protein were proved by IFA and Western blot. We successfully constructed a mammalian cell line which stably expressed M2 protein of influenza virus type A. The cell line will be useful for studies on function of M2 protein and provide tools for novel influenza virus vaccine development.
Animals
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CHO Cells
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Cell Culture Techniques
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Cell Line
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Cricetinae
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Cricetulus
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Influenza A Virus, H2N2 Subtype
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chemistry
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Recombinant Proteins
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biosynthesis
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Viral Matrix Proteins
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biosynthesis
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genetics