4.Determination of indiumin urine by inductively coupled plasma mass spectrometry.
Yiran LIN ; Juntao HE ; Xiaozuo XU ; Jiangwei YIN ; Liuzhuo ZHANG ; Dafeng LIN ; Zhimin LI ; Juan YI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(8):630-631
Humans
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Indium
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urine
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Mass Spectrometry
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methods
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Spectrum Analysis
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methods
9.Determination of indium in whole blood by graphite furnace atomic absorption spectrometry.
Juntao HE ; Fen LIU ; Yingping XIANG ; Zhimin LI ; E-mail: LIZHIMIN567@SINA.COM.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(8):627-629
OBJECTIVETo investigate the sensitization effect of different chemical modifiers in the determination of indium in whole blood by graphite furnace atomic absorption spectrometry, and to develop a new method for the determination of indium in whole blood.
METHODSA mixture of 0.3% HNO3 (V/V) + 0.1% Triton X-100 (V/V) was used as a diluent, and a solution of 1 000 µg/ml Pd (NO3)2 + 3 000 µg/ml Mg (NO3)2 was used as modifier. After being diluted five times, the concentration of indium of the blood was directly determined by graphite furnace atomic absorption spectrometry.
RESULTSThe detection limit of the method was 0.33 µg/L, the linear range was 0.33~100.00 µg/L, the relative standard deviation was 1.43%~2.65%, and the recovery rate was 98.3%~105.3%.
CONCLUSIONThe method is simple and fast and has high recovery and precision, and it is suitable for the determination of indium in whole blood.
Blood Chemical Analysis ; instrumentation ; Graphite ; Humans ; Indium ; blood ; Limit of Detection ; Spectrophotometry, Atomic ; methods
10.Effects of indium on micronucleus formation in human peripheral blood lymphocytes.
Yan GUO ; Changye HUI ; Liuzhuo ZHANG ; Lili WANG ; Dianpeng WANG ; Xueqin YANG ; Xinyue YANG ; Zhimin LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(8):563-565
OBJECTIVETo investigate the cytotoxicity of indium chloride (InCl₃) and its effects on micro-nucleus formation in primary human lymphocytes cultured in vitro.
METHODSThe CCK-8 assay was used to evaluate the cytotoxicity of 24 h exposure to different concentrations of InCl₃(4, 40, 80, 200, 500, and 1 000 µmol/L) in lymphocytes cultured in vitro. The cytokinesis-block method was used to determine the micronucleus level in lymphocytes exposed to different concentrations of InCl₃and the effects of anti-oxidant vitamin C on micronucleus frequency.
RESULTSLymphocytes exposed to InCl₃of no less than 500 µmol/L had significantly lower survival rates than those in the control group (P < 0.05). Lymphocytes exposed to 80 µmol/L InCl₃had a significantly higher micronucleus frequency than those in the control group (P < 0.05). However, there was no further increase in micronucleus frequency of lymphocytes exposed to 200 µmol/L InCl₃. Lymphocytes cultured in whole blood and exposed to 500 or 1000 µmol/L InCl₃had a significantly increased micronucleus frequency than those in the control group (P < 0.001). The increase in micronucleus frequency of lymphocytes induced by indium could be partially antagonized by 20 or 100 µmol/L vitamin C.
CONCLUSIONInCl₃can induce an increase in micronucleus frequency of primary human lymphocytes cultured in vitro, which might be associated with DNA damage induced by oxidative stress.
Cell Nucleus ; metabolism ; Cytokinesis ; DNA Damage ; Humans ; In Vitro Techniques ; Indium ; toxicity ; Lymphocytes ; drug effects ; Oxidative Stress
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