Treatment of rabbits, exposed to mix of yperite and lewisite with unithiol and naturenze showed that unithol reduced concentration of SGOT (serum glutamat-oxaloacetat-transaminase), SGPT (serum glutamat-pyruvat-transaminase), urea and creatinine in serum but concentration of these components was still high. Using unithiol in combination with naturenze for treatment, concentration of SGOT, SGPT, urea and creatinine was decreased lower than treatment of single unithiol.
Unithiol ; Toxicity ; Indicators and Reagents

Concentration of SGOT, SGPT, ure and creatinine was increased in serum of rabbits, exposed to diclodietylsulfid and clovinyldiclorasin. We realized that there were synergic effects between diclodietylsulfid and clovinyldiclorasin, caused increase of ure and creatinie in rabbits higher than effect of single agent. Concentration of glucose was increased at the third day and decreased at the 7th day in rabbits exposed to mix of clovinyldiclorasin and diclodietylsulfid.
Indicators and Reagents ; Toxicity

Nitric oxide (NO) is a non-adrenergic, non-cholinergic neurotransmitter found in the enteric nervous system that plays a role in a variety of enteropathies, including inflammatory bowel disease. Alteration of nitrergic neurons has been reported to be dependent on the manner by which inflammation is caused. However, this observed alteration has not been reported with acetic acid-induced colitis. Therefore, the purpose of the current study was to investigate changes in nitrergic neuromuscular transmission in experimental colitis in a rat model. Distal colitis was induced by intracolonic administration of 4% acetic acid in the rat. Animals were sacrificed at 4 h and 48 h postacetic acid treatment. Myeloperoxidase activity was significantly increased in the acetic acid-treated groups. However, the response to 60 mM KCl was not significantly different in the three groups studied. The amplitude of phasic contractions was increased by Nomega-nitro-L-arginine methyl ester (L-NAME) in the normal control group, but not in the acetic acid-treated groups. Spontaneous contractions disappeared during electrical field stimulation (EFS) in normal group. However, for the colitis groups, these contractions initially disappeared, and then reappeared during EFS. Moreover, the observed disappearance was diminished by L-NAME; this suggests that these responses were NO-mediated. In addition, the number of NADPH-diaphorase positive nerve cell bodies, in the myenteric plexus, was not altered in the distal colon; whereas the area of NADPH-diaphorase positive fibers, in the circular muscle layer, was decreased in the acetic acidtreated groups. These results suggest that NO-mediated inhibitory neural input, to the circular muscle, was decreased in the acetic acid-treated groups.
Acetic Acid/toxicity ; Animals ; Colitis/chemically induced/*pathology/*physiopathology ; Colon/drug effects/enzymology/*innervation/pathology ; Indicators and Reagents/toxicity ; Male ; Muscle Contraction/drug effects ; Muscle, Smooth/drug effects/metabolism ; Myenteric Plexus/pathology ; NADPH Dehydrogenase/metabolism ; NG-Nitroarginine Methyl Ester/pharmacology ; Neuromuscular Junction/drug effects/*metabolism ; Nitrergic Neurons/drug effects/*metabolism ; Nitric Oxide/*metabolism ; Peroxidase/metabolism ; Potassium Chloride/pharmacology ; Rats ; Rats, Sprague-Dawley