1.Idiopathic intranuclear inclusion bodies in the renal tubular epithelia of Japanese quail (Coturnix coturnix japonica).
Seung Hyeok SEOK ; Jong Hwan PARK ; Sun A CHO ; Jae Hak PARK
Journal of Veterinary Science 2005;6(1):75-76
We report idiopathic intranuclear inclusion bodies in the renal tubular epithelia of two cases of among the 960 Japanese quail (Coturnix coturnix japonica) in the course of the acute oral toxicity and dietary toxicity test. Basophilic inclusion bodies were seen only in the nuclei of renal tubular epithelia. We could not classify our case into any adenovirus infection by clinical signs and lesions. The inclusion bodies were only identified as adenovirus-like particles based upon the electronmicroscopical features.
Animals
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*Coturnix
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Epithelial Cells/*ultrastructure
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*Intranuclear Inclusion Bodies
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Kidney Tubules/*ultrastructure
2.Ultrastructure of Chronic Liver Diseases: Mallory Body of the Hepatocyte.
The Korean Journal of Hepatology 2003;9(1):49-66
No abstract available.
Chronic Disease
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Hepatocytes/*ultrastructure
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Humans
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Inclusion Bodies/*ultrastructure
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Liver Diseases/*pathology
3.The discovery of naked cluster particles of Parachlamydia and its developmental mechanism.
Qin-xue LI ; Qing-wu JIANG ; Jie SHEN ; Zi-hua LI
Chinese Journal of Epidemiology 2004;25(10):878-881
OBJECTIVETo study the survival and developmental morphology of Parachlamydia (BN9) within Acanthamoeba.
METHODSThe morphology of BN9 within Acanthamoeba was studied by inverted phase contrast microscope, electron microscope, Gimenez and AO-staining with amoebal co-culture.
RESULTSThe endosomal maturation-blocked were formed after the egress of BN9. Two developmental stages-elementary and reticulate bodies, were both observed within the vacuoles. The reticulate bodies, multiplicated by binary fission, were located mainly within the vacuoles, while the elementary bodies can also be located in the plasma individually. The naked cluster particles were observed after the trophozoites cytolysis with Gimenez-staining. The light infectious trophozoites could encyst, and elementary bodies could survive within the mature cysts.
CONCLUSIONThe egress of BN9 could form the endosomal maturation-blocked, which was presented in two developmental stages-elementary and reticulate bodies. It exhibited the cytolysin activity that could lyse the infectious trophozoites and were expelled in the vesicles. A few light infected amoeba could encyst with survival elementary bodies in the plasma.
Acanthamoeba ; microbiology ; ultrastructure ; Animals ; Chlamydiales ; physiology ; ultrastructure ; Coculture Techniques ; Humans ; Inclusion Bodies ; ultrastructure ; Life Cycle Stages ; Microscopy, Electron
4.Inclusion Bodies are Formed in SFTSV-infected Human Macrophages.
Cong JIN ; Jingdong SONG ; Ying HAN ; Chuan LI ; Peihong QIU ; Mifang LIANG
Chinese Journal of Virology 2016;32(1):19-25
The severe fever with thrombocytopenia syndrome virus (SFTSV) is a new member in the genus Phlebovirus of the family Bunyaviridae identified in China. The SFTSV is also the causative pathogen of an emerging infectious disease: severe fever with thrombocytopenia syndrome. Using immunofluorescent staining and confocal microscopy, the intracellular distribution of nucleocapsid protein (NP) in SFTSV-infected THP-1 cells was investigated with serial doses of SFTSV at different times after infection. Transmission electron microscopy was used to observe the ultrafine intracellular structure of SFTSV-infected THP-1 cells at different times after infection. SFTSV NP could form intracellular inclusion bodies in infected THP-1 cells. The association between NP-formed inclusion bodies and virus production was analyzed: the size of the inclusion body formed 3 days after infection was correlated with the viral load in supernatants collected 7 days after infection. These findings suggest that the inclusion bodies formed in SFTSV-infected THP-1 cells could be where the SFTSV uses host-cell proteins and intracellular organelles to produce new viral particles.
Cell Line
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China
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Humans
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Inclusion Bodies, Viral
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ultrastructure
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virology
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Macrophages
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ultrastructure
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virology
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Phlebotomus Fever
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virology
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Phlebovirus
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genetics
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physiology
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ultrastructure
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Thrombocytopenia
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virology
5.Intranuclear Cytoplasmic Pseudoinclusions in Pituitary Adenomas.
Seok Woo YANG ; Kyung Moo YANG ; Hae Youn KANG ; Tai Seung KIM
Yonsei Medical Journal 2003;44(5):816-820
Intranuclear pseudoinclusions are well known in papillary carcinomas of the thyroid gland, hepatocellular carcinomas, meningiomas, paragangliomas, pheochromocytomas, and melanomas. Only two papers on the intranuclear inclusions of adenohypophyseal cells in humans have been reported. This study found that intranuclear cytoplasmic pseudoinclusions occur frequently in pituitary adenoma cases (70.3%, 97 of 138 pituitary adenomas) and are uncommon in normal pituitary tissue (11.1%, 1 of 9 normal pituitary tissues). In addition, the frequency of intranuclear cytoplasmic pseudoinclusions between the functional and non-functional pituitary adenomas was found to be similar. Electron microscopy and immunostaining was used to reveal the entity of the intranuclear inclusion. These intranuclear inclusions are due to cytoplasmic invagination because 1) the inclusions are continuous with the cytoplasm, 2) all cytoplasmic organelles, such as the endoplasmic reticulum, the Golgi apparatus, and the secretory granules are found in the inclusions, 3) immunoreactivity of the intranuclear inclusion is the same as that of the cytoplasm. In conclusion, intranuclear cytoplasmic pseudoinclusions in pituitary adenomas occur frequently (70.3%) and are formed by cytoplasmic invagination. This study suggests that pituitary intranuclear inclusions caused by cytoplasmic invagination be called "intranuclear cytoplasmic pseudoinclusions".
Adenoma/pathology/*ultrastructure
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Adolescent
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Adult
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Aged
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Female
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Human
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Intranuclear Inclusion Bodies/*ultrastructure
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Male
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Microscopy, Electron
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Middle Aged
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Pituitary Neoplasms/pathology/*ultrastructure
6.Pale bodies in hepatocellular carcinoma.
Woo Sung MOON ; Hee Chul YU ; Myoung Ja CHUNG ; Myung Jae KANG ; Dong Geun LEE
Journal of Korean Medical Science 2000;15(5):516-520
Histochemical, immunohistochemical and ultrastructural studies were performed on cases of hepatocellular carcinoma (HCC) with pale bodies (PB). HCC containing PBs was observed in 3 (5.5%) of 55 consecutively resected HCC cases. Histologically, a large number of hepatocytes displayed pale or eosinophilic staining of the cytoplasm, resulting in ground-glass appearance. They were aggregated in nodular pattern, or diffusely intermixed with other malignant hepatocytes. PBs were negative for periodic-acid Schiff and Masson's trichrome staining. The inclusions showed a strong positive reaction for fibrinogen and some of them were weakly positive for albumin but negative for hepatitis B surface antigen, hepatitis B core antigen, alpha-fetoprotein and alpha-1-antitrypsin. Ultrastructurally, PBs were membrane-bound and contained granular materials of moderate electron density, and were closely related to dilated rough endoplasmic reticulum. These findings support that PBs are secretory fibrinogen accumulated in cystic ER and that such intracellular accumulation possibly reflects a defective transport of fibrinogen.
Albumins/analysis
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Carcinoma, Hepatocellular/pathology*
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Cytoplasm/ultrastructure
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Cytoplasm/pathology
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Cytoplasm/chemistry
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Endoplasmic Reticulum, Rough/ultrastructure
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Endoplasmic Reticulum, Rough/pathology
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Endoplasmic Reticulum, Rough/chemistry
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Fibrinogen/analysis
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Human
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Inclusion Bodies/ultrastructure
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Inclusion Bodies/pathology*
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Inclusion Bodies/chemistry
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Liver Neoplasms/pathology*
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Male
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Microscopy, Electron
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Middle Age
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Periodic Acid-Schiff Reaction
7.Characterization of fibrillous inclusion body (FIB) of human adenovirus type 41 (Ad41) using immunoelectron microscopy.
Jing-Dong SONG ; Xiao-Hui ZOU ; Min WANG ; Jian-Guo QU ; Zhuo-Zhuang LU ; Tao HONG
Chinese Journal of Virology 2013;29(6):596-601
To investigate the components of fibrillous inclusion body (FIB), which was formed in packaging cells during the replication of human adenovirus type 41 (Ad41), Ad41 long fiber knob (LFK) and short fiber knob (SFK) proteins were expressed in prokaryote respectively and then used to immunize BALI mice for preparation of anti-LFK serum and anti-SFK sera. The activity and specificity of anti-LFK and an ti-SFK sera were confirmed with Western blot, indirect immunofluorescence assay (IFA) and immunonegative staining, suggesting these sera could be applied in immuno-colloidal gold labelling electron microscopy (EM). 293TE cells were infected with wild-type Ad41. Ultrathin sections of infected cells were made, and labelled with immuno-colloidal gold technique using anti-Ad41 sera, anti-LFK sera, anti-SFK sera, or anti-fiber monoclonal antibody 4D2, respectively. The labelled sections were observed under EM, and the results demonstrated that both Ad41 long fiber protein and short fiber protein were included FIB.
Adenovirus Infections, Human
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virology
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Adenoviruses, Human
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genetics
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metabolism
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ultrastructure
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Animals
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Female
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Humans
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Inclusion Bodies, Viral
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ultrastructure
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Mice
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Mice, Inbred BALB C
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Microscopy, Immunoelectron
8.The clinicopathological study of infantile cytomegalovirus hepatitis.
Yuan-Ting TANG ; Xiao-Qin GUAN ; Rui-Qiu ZHAO
Chinese Journal of Hepatology 2009;17(1):21-23
OBJECTIVETo investigate the clinicopathological features of infantile cytomegalovirus hepatitis.
METHODLiver biopsies from 30 cases of infantile cytomegalovirus hepatitis were observed under optical microscope and electronic microscope.
RESULTThe main clinical manifestations were jaundice, splenohepatomegaly and hypohepatia. Laboratory test showed dysfunction of liver, high level of CMV DNA, and high titer of anti-CMV antibody. Imaging examination demonstrated hepatomegaly. The histological changes were hepatocellular degeneration, necrosis, apoptosis, and fibrosis. The histological characteristics of cytomegalovirus hepatitis, including intranuclear inclusions in multinucleated giant cells and pseudo-lumens, were also observed under optical microscope. In addition, virion was observed in the nuclei and cytoplasm of hepatocytes under electronic microscope.
CONCLUSIONThe viral DNA and serological tests have limited utility for the diagnosis of infantile cytomegalovirus hepatitis, and the final diagnosis depends on histopathology.
Biopsy, Needle ; Cytomegalovirus Infections ; pathology ; Female ; Hepatitis, Viral, Human ; pathology ; Hepatocytes ; pathology ; ultrastructure ; Humans ; Inclusion Bodies, Viral ; pathology ; Infant ; Infant, Newborn ; Liver ; pathology ; Male ; Mitochondria, Liver ; pathology ; ultrastructure
9.A Case of IgA kappa Light Chain Deposition Disease and Combined Adult Fanconi Syndrome with Auer rod-like Intracytoplasmic Inclusions in Plasma Cells and Proximal Renal Tubular Cells.
Jimin KAHNG ; Jeana KIM ; Suk Joon SHIN ; Kyungja HAN
The Korean Journal of Laboratory Medicine 2007;27(4):248-252
We report a case of IgA kappa light chain deposition disease and combined adult Fanconi syndrome with Auer rod-like intracytoplasmic inclusions in plasma cells and proximal renal tubular cells in a 54-yr-old female. Cytochemical stainings revealed a strong acid phosphatase activity of the inclusions and weak periodic acid-Schiff positivity, whereas the reactions for peroxidase and alpha-naphthyl acetate esterase were negative. An immunostaining verified IgA-kappa inside the plasma cells. Kidney biopsy revealed Bence Jones cast nephropathy with kappa light chain positivity, and Congo red staining was negative. Electron microscopy showed needle-shaped crystals located in tubular epithelial cells.
Fanconi Syndrome/diagnosis/etiology/*pathology
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Female
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Humans
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*Immunoglobulin A/analysis
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Immunoglobulin kappa-Chains/analysis
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Inclusion Bodies/*ultrastructure
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Kidney Tubules, Proximal/pathology/*ultrastructure
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Middle Aged
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Paraproteinemias/*pathology
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Plasma Cells/pathology/*ultrastructure
10.Relationship between the pathologic changes of human embryo fibroblast cells and expression of late mRNA after human cytomegalovirus infection in vitro.
Hui WANG ; Liang-zhen WEN ; Xia-zhen LING
Chinese Journal of Experimental and Clinical Virology 2004;18(4):351-353
OBJECTIVETo study the relationship between late mRNA and the cytopathic effect(CPE) and ultrastructural features after human cytomegalovirus (HCMV) infection in vitro.
METHODSHuman embryo fibroblast cells(HEL) were infected with HCMV AD169 strain. The expression of the HCMV late mRNA was measured by semiquantitative RT-PCR, the cytopathic effect (CPE) and the cell ultrastructure were observed by means of light microscopy and electron microscopy, respectively.
RESULTSThe HCMV late mRNA could be detected 12 hours postinfection and increased gradually, but the CPE appeared 48 hours postinfection in HEL cells. The HCMV infected cells exhibited significant mitochondrial enlargement and the number of mitochondrial ridge deletion, the cisternae lumen of endoplasmic reticulum (ER) dilation and vacuolization (at the end age). The mature nucleocapsid could be observed 96 hours postinfection.
CONCLUSIONThe ultrastructural changes have an intimate correlation with the expression of HCMV late mRNA and play an important role in the life circle of the virus. HCMV late mRNA may serve as a indicator of the clinical effect of treatment in active HCMV infection.
Cytomegalovirus ; genetics ; physiology ; Cytopathogenic Effect, Viral ; Embryo, Mammalian ; Endoplasmic Reticulum ; pathology ; virology ; Fibroblasts ; metabolism ; ultrastructure ; virology ; Humans ; Inclusion Bodies ; pathology ; virology ; Mitochondrial Swelling ; RNA, Messenger ; metabolism