[Objective]To study the regulation effects of Oridonin on pulmonary artery pressure in pulmonary hypertension rats with hypoxia-hypercapnia and its mechanism.[Methods]SD rats were divided in to three groups randomly: the normal control group(NC),the hypoxia-hpercapnia group(HH),and the hypoxia-hpercapnia plus Oridonin group(O).The time of hypoxia-hypercapnia was 4 weeks.The mean pulmonary artery pressure(mPAP),right ventricular rate(RV/(LV+S)),percentage of thickness of pulmonary artery wall in vascular external diameter(WT%),percentage of pulmonary artery wall area in total vascular area(WA%),vitality of caspase3 and caspase9,expression cyt-c of each group were observed.[Results]The mPAP,RV/(LV+S),WT%,WA% in group HH were significantly higher than those in group NC(P

Renal scarring can cause hypertension, proteinuria, chronic kidney disease, and even end stage renal disease. To understand the risk factors of renal scarring in children is helpful for its early detection, diagnosis, prevention, and treatment. High grade vesicoureteric reflux, recurrent urinary tract infection, and delayed treatment are risk factors for kidney scarring. However, there are still some controversies about the relationship between renal scarring and the some factors such as gender, age, and congenital factors. Recent studies have found that noninvasive urine indicators such as urine neutrophil gelatinase associated lipid delivery protein, urinary endothelin-1, and risk prediction models can predict the formation of renal scar. However, prophylactic use of antibiotics can reduce occurance of urinary tract infections, but do not reduce the risk of renal scarring. This article reviews the possible risk factors and predictors of renal scarring in children, providing a basis for early detection, timely intervention, and effective prevention of it in clinic, so as to reduce the formation and progression of renal scarring.

Administration, Topical ; Humans ; Immunosuppressive Agents ; administration & dosage ; adverse effects ; therapeutic use ; Lichen Planus, Oral ; complications ; drug therapy ; pathology ; Oral Ulcer ; drug therapy ; etiology ; Tacrolimus ; administration & dosage ; adverse effects ; therapeutic use ; Treatment Outcome

Ambulatory blood pressure monitoring(ABPM)system can record patients blood pressure of 24 h on the predetermined time. The primary data forms the graph,the curve and the general report by operation of the related software after the analysis and statistics processing. Patients keep daily life condition,so the blood pressure data is more accuracy. ABPM can provide the dynamic change of the patients 24 h blood pressure,including the 24 h blood pressure survey data,the undulation situation and the change tendency. ABPM has become an useful element in the evaluation and follow - up of hypertension in adults. And ABPM is increasingly used to evaluate the blood pressure of children and adolescents in recent years. The ABPM has been shown to differ significantly values.

Objective To observe the clinical efficacy of triple needling and centro-square needling in treating hemifacial spasm. Method Sixty-eight patients with facial spasm were randomized into a treatment group and a control group, 34 in each group. The treatment group was intervened by centro-square needling at topical Ashi points and triple needling at Yifeng (TE 17) on the affected side; the control group was intervened by regular acupuncture. The two groups were treated once a day, 10 times as a treatment course, for 3 courses in total. Cohen Albert grading scale was adopted to evaluate the spasm intensity before and after the intervention, and the clinical efficacies were then compared.Result After intervention, there was a significant difference in comparing the spasm intensity between the two groups (P<0.05). The significant improvement rate was 88.2% in the treatment group versus 50.0% in the control group, and the difference was statistically significant (P<0.05).Conclusion Triple needling plus centro-square needling is an effective method in treating hemifacial spasm. This method uses less points, but it produces a high efficacy, strong pertinence, and is easy-to-operate.

BacKground:Lymphocyte function-associated antigen-1( LFA-1 ) pIays a cruciaI roIe in the pathogenesis of infIammatory boweI disease by reguIating the activation and function of T ceIIs. Aims:To investigate the effect of LFA-1 deficiency( LFA-1-/-)on differentiation of mice na?ve T ceIIs into Th17 ceIIs in vitro. Methods:LFA-1-/- mice were breeded and the progeny genome DNA was extracted from the taiIs for genotyping by PCR. CD4+CD62L+ na?ve T ceIIs were separated from spIenic mononucIear ceIIs of LFA-1-/- progeny mice and wiId type ( WT ) C57BL/6J mice, respectiveIy,by magnetic-activated ceII sorting( MACS),and then the purity of separated ceIIs was determined. Na?ve T ceIIs obtained were cuItured in different inducing systems[ transforming growth factor-β( TGF-β),TGF-β + interIeukin-6 (IL-6),and TGF-β + IL-6 + IL-23]in vitro for Th17 ceII differentiation;ceIIs in each inducing system were coIIected for anaIyzing the ratio of Th17 ceIIs by fIow cytometry,and the expressions of Th17-specific transcription factor ROR-γt and Th17-specific marker IL-17A were measured by qRT-PCR and ELISA methods. Results:AII fifteen progeny mice were identified as LFA-1-/- genotype. Purity of CD4+CD62L+ na?ve T ceIIs separated by MACS was above 95%. Th17 ceIIs couId be induced by Iow-dose TGF-βcombined with IL-6,and the differentiation ratio was increased obviousIy when IL-23 was added. In inducing system containing TGF-β,IL-6 and IL-23,na?ve T ceIIs from LFA-1-/- mice produced more Th17 ceIIs than those from WT mice(17. 2% ± 1. 4% vs. 5. 7% ± 0. 2%,P<0. 001),expressions of ROR-γt mRNA and IL-17A mRNA were up-reguIated(P<0. 001),and IL-17A concentration in ceII cuIture supernatant was increased(P<0. 01). Conclusions:Deficiency of LFA-1 promotes the differentiation of mice na?ve T ceIIs into Th17 ceIIs in vitro.

Vascular dementia is one of the common causes of cognitive impairment.Occlusion of bilateral common carotid arteries, middle cerebral artery occlusion, as well as induced hypertension are major methods to make rat model for vascular dementia.The present review will summarize research progress in medical and non-medical treatment of vascular dementia rat models in order to provide theoretical direction for clinical practice.

Objective:To establish an HPLC method for the simultaneous determination of methionine, vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules. Methods:The determination was performed on a Ultimate? AQ-C18 column(250 mm × 4. 6 mm, 5 μm,Welch Inc. ) with the detection wavelength at 220 nm. The mobile phase was composed of methanol and 0. 07 mol·L-1 sodium heptanesulfonate solution (14 ml triethylamine diluted with water to1 000ml, adjusting pH to 3. 5 with diluted orthophosphoric acid) with gradient elution, and the flow rate was 1. 0 ml·min-1. Results: Methionine, vitamin B1 and vitamin B2 was in good linearity within the range of 0.527 0-1.510 0 mg·ml-1 - (r =0.999 9), 0.045 52-0.136 56 mg·ml-1 (r =0.999 9) and 0.010 10-0. 060 58 mg·ml-1(r=0. 999 9), respectively. The mean recovery was 100. 5%, 97. 7% and 101. 5% with RSD of 0. 5%, 1. 0%and 1. 4%(n=9) accordingly. Conclusion:The method is simple, accurate, reliable and appropriate in the simultaneous determina-tion of methionine,vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules.

In the treatment of 169 cases of the patients sick with intervertebral disc hemiation by integrated application of acupuncture therapy,Chinese massage therapy and herbal fumigation method.the results showed cure in 129 cases.improvement in 33 cases and failure in 7 cases,and the total effective rate was 95.9%.

ObjectiveTo study the clinical effect of sodium aescinate on the edema of limb tibial plate fracture.Methods69 patients with edema of limb of tibial plate fracture were randomly divided into two groups,treatment group(46 cases)and control group(23 cases).Both of them were transfused with 20％ mannitol(250ml,Bid ×7days).In addition,these patients in the treatment group were treated with sodium aescinate(30mg Qd × 7days).ResultsThe repercussive effect of the edema grade in the treatment group were obviously better than those in the control group(P ＜0.05 ).ConclusionSodium aescinate had a remarkable repercussive effect on the limb edema of tibial plate fracture.