Selection of the surgical procedure for Hallux Valgus is decided after variable factors including radiologic indices were considered. Among them, congruency of the first metatarsophalangeal [MTP] joint is important factor to get the better prognosis. Articular surface angle of first metatarsal head [AAMH] has been used to substituted for first MTP joint congruency. But there had been questions whatever that the value of AAMH measured radiologically equal to real angle in surgical field. The purpose of this article is to compare clinically used radiologic angle of AAMH to real anatomical angle in patients operated by. From march to october 1998, among the 75 patients operated by surgical method (Proximal Chevron), measures anatomical AAMH of 25 volunteer at random. Average age was 46.4 yrs old, ratio of the sample is Right : Left = 9 : 16. The radiologic AAMH measured by AOFAS (American Orthopedic Foot and Ankle Society) research committee method 1992 in the weight bearing foot AP film. The actual AAMH is measured directly in the operative field. After the capsular incision had done, first metatarsal head was dislocated totally. K -wire was inserted to articular dome of the metatasal head from medial to lateral, The transverse axial plane of K -wire is parallel to plantigrade foot position. Afterthen, printed image was taken using portable image intensifier. Average radiologic value of metatarsal head was 16.8 degree, real value was 23.52 degree. In general, real angle was bigger than radiologic angle. In the 20% of cases, radiologic angle was bigger than real angle. With paired t -test, there was no correlations in each angles (p>0.01). Conclusion; The average real AAMH measured in surgical field was 23.1degree. There was no statistic correlations between the angles radiologically and anatomically. therefore, We conclude that the angle of distal metatarsal articular surface measured radiologically has not the clinical significance to know articular congruency. Consequently We suggested the three dimensional study to know the real angle of articular surface of first metatarsal head clinically as alternative method.
Ankle ; Foot ; Hallux Valgus ; Head ; Humans ; Joints ; Metatarsal Bones* ; Orthopedics ; Prognosis ; Volunteers ; Weight-Bearing

No abstract available.
External Fixators* ; Foot Deformities* ; Foot*

This study was performed to investigate the morphology of the enteric nervous system and interstitial cells of Cajal (ICC) in the murine ileum. The PGP9.5-like immunoreactive (PGP9.5-LI) neurons and the c-Kit-like immunoreactive (c-Kit-LI) ICCs were stained by indirect immunofluorescence method and were observed under the confocal laser scanning microscopy. According to three dimensional reconstruction study, it was found that the PGP9.5-LI neurons and the c-Kit-LI ICCs were widely distributed in the intestinal wall : (1) In circular muscle layer, PGP9.5-LI nerve fibers were paralell to circular muscle layer. (2) In the myenteric plexus, the PGP9.5-LI nerve were closely apposed to the adjacent PGP9.5-LI nerve, constituting the networks. (3) In double-labeling immunohistochemistry using anti-PGP9.5 and anti-c-Kit antibodies, the c-Kit-LI networks encircled around the neural strands. The characteristic arrangement of the PGP9.5-LI enteric nervous system and the ICC containing c-Kit-positive cells provide a morphological basis upon the mechanism regulating gastro-intestinal motility and the pathogenesis of gatro-intestinal disorders.
Animals ; Antibodies ; Enteric Nervous System* ; Fluorescent Antibody Technique, Indirect ; Ileum* ; Immunohistochemistry ; Interstitial Cells of Cajal* ; Mice ; Microscopy, Confocal* ; Myenteric Plexus ; Nerve Fibers ; Neurons

The purpose of this study is to present a novel method of harvesting autogenous bone graft and to analyze the behavior of xenograft used for pelvic osteotomy in young children. Twenty hips of eighteen patients underwent pelvic osteotomies using xenograft (Lubhoc) from Sep. 1993 to Jun. 1996. In fitteen hips, we harvested autogenous bone avoiding damage to the chondroapophysis of iliac crest and t'illed the donor site with the xenograft. It supplemented the autogenous bone at the osteotomy site in eleven hips, and was used as a wedge without autogenous hone in five hips. During the followup, no growth disturbance of iliac crest was found. The xenograft incorporation was satisfactory at the graft donor sites and the osteotomy sites where it supplemented the autogenous bone, however, unsatistactory at the osteotomy sites where it was used alone. Our novel method of harvesting bone graft from young pelvis may help prevent growth disturbance of lilac crest, and Luhhoc is useful as space-filler of donor site and suppiementary to the autograft in pelvic osteotomy of young children.
Autografts ; Child* ; Follow-Up Studies ; Heterografts* ; Hip ; Humans ; Osteotomy* ; Pelvis ; Tissue Donors ; Transplants

Astragalus membranaceus is used as a natural herbal medicine in East Asia for preventing carcinogenesis and reducing side effects induced by chemotherapy in cancer patients. Although the mechanism of anti-tumor activity is not known, the polysaccharides may potentiate the host defense mechanism through the activation of immune system. The objective of this study is to investigate the mechanism by which APS activates macrophages. To analyze macrophage activation and iNOS gene expression, we performed nitrite generation assay, immunohistochemistry, and RT-PCR. In the present study we show that a polysaccharide isolated from the Astragalus membranaceus (Astragalus Polysaccharide, APS) significantly induces nitric oxide (NO). Immunohistochemical staining of inducible NO synthase (iNOS) showed that the increase of NO was due to the induction of iNOS production. To further study the mechanism responsible for the induction of iNOS, we investigated the effect of APS on the iNOS mRNA expression. RT-PCR analysis showed that APS produced significant induction of iNOS gene expression. In conclusion, we demonstrate that a polysaccharide isolated from Astragalus membranaceus stimulates macrophages to generate NO through the activation of iNOS gene expression.
Astragalus membranaceus* ; Carcinogenesis ; Drug Therapy ; Far East ; Gene Expression ; Herbal Medicine ; Humans ; Immune System ; Immunohistochemistry ; Macrophage Activation ; Macrophages ; Nitric Oxide ; Nitric Oxide Synthase ; Polysaccharides ; RNA, Messenger

Authors have anthropologically measured the human skeleton from a Dugmoe Tomb of the technopolis of Kwang-ju city. The results obtained were as follows : 1. The human skeleton was thought to be constructed at the beginning of the period of the Chosun, judging from the Dugmoe Tomb. 2. It is female and its stature is estimated as about 163-165cm. The age of the skeleton is estimated to be late 50. 3. The cranial index is 77.09mm and the type is mesocephaly. 4. The cranial length-height index and the cranial breadth-height index were hypsicrane and acrocephaly. 5. The orbital index 95.0mm and the type is hypsiconch. 6. The humerus is comparatively long, slender and has more rounded diaphysis. 7. The femur is similar that of present day, and the type is platyer. 8. The abrasion of the occlusal surface of the teeth was 2.5 point.
Craniosynostoses ; Diaphyses ; Female ; Femur ; Gwangju* ; Humans* ; Humerus ; Orbit ; Skeleton* ; Tooth

The sclerotium of Poria cocos Wolf, which grows on the roots of pine trees, has long been used as a sedative, diuretic, and anti-inflammatory agent. The accumulating data revealed that certain ingredients of the sclerotium of Poria cocos showed anti-tumor activities. Although the mechanism of anti-tumor activity is not known, the polysaccharides may potentiate the host defense mechanism through the activation of immune system. In the present study we show that PCSC22, a polysaccharide isolated from the sclerotium of Poria cocos with one percent sodium carbonate, significantly induces nitric oxide (NO). Immunohistochemical staining of inducible NO synthase (iNOS) showed that the increase of NO was due to the induction of iNOS production. To further study the mechanism responsible for the induction of iNOS gene expression, we investigated the effect of PCSC22 on the activation of p38 kinase, which is important in the gene expression of inflammatory cytokines including iNOS. Western blot assay showed that PCSC22 produced phosphorylation of p38 kinase. In conclusion, we demonstrate that PCSC stimulates macrophages to express iNOS gene through the activation of p38 kinase.
Blotting, Western ; Carbon ; Cocos* ; Cytokines ; Gene Expression ; Immune System ; Macrophages* ; Nitric Oxide ; Nitric Oxide Synthase ; Phosphorylation ; Phosphotransferases ; Pinus ; Polysaccharides ; Poria* ; Sodium ; Wolves

Calcium-binding proteins play an important role in the protection, differentiation, and reorganization of the central nervous system. The effects of neonatal retinal deafferentiation on calretinin, and tracing of retinotectal pathway were examined immunohistochemically in the superficial layer of the rat superior colliculus. Tracing with cholera toxin was revealed on the superior colliculus contralateral to the ocular injection. On the contralateral side of superior colliculus, the calretinin-immunoreactive (IR) cells were dramatically increased, calretinin-IR fibers were markedly decreased in the superficial layer. These results show that retinal deafferentation results in an increase of calretinin-immunoreactive cells within the superficial layers of the superior colliculus, which suggest reorganization of neurons in superior colliculus.
Animals ; Calbindin 2 ; Calcium-Binding Proteins ; Central Nervous System ; Cholera Toxin ; Eye Enucleation* ; Neurons ; Rats* ; Retinaldehyde ; Superior Colliculi

The vanilloid receptor type-1 (VR1) is a nonselective cation channel activated by capsaicin and can be act as mediator of chemical and physical stimuli that elicit pain. The presence of VR1 in the dorsal root, trigeminal and nodose ganglia has been firmly established, but it unclear in the mouse intestinal wall. The distribution of VR1 receptors in mouse afferent neurons innervating the intestinal tract was investigated by immunohistochemistry. Also small and large intestines were dual-labelled with antibody for VR1 and marker for interstitial cells of Cajal (c-kit). VR1-immunopositive cells were localized on fine fibers in myenteric plexus and expressed weakly myenteric ganglia. The majority of VR1-immunopositive fibers are not colocalized with or apposed to c-kit positive interstitial cells of Cajal. Also electrophysiologically capsaicin had no effect on cultured interstitial cells of Cajal. It is concluded that VR1-immunoreactive intestinal nerves are mainly distributed in myenteric plexus of murine intestinal wall, and vanillod may be not directly related to interstitial cells of Cajal in regulation of intestinal motility.
Animals ; Capsaicin ; Ganglia ; Gastrointestinal Motility ; Immunohistochemistry ; Interstitial Cells of Cajal ; Intestines ; Mice* ; Myenteric Plexus* ; Neurons, Afferent ; Nodose Ganglion ; Spinal Nerve Roots

The vanilloid receptor type-1 (VR1) is a nonselective cation channel activated by capsaicin and can be act as mediator of chemical and physical stimuli that elicit pain. The presence of VR1 in the dorsal root, trigeminal and nodose ganglia has been firmly established, but it unclear in the mouse intestinal wall. The distribution of VR1 receptors in mouse afferent neurons innervating the intestinal tract was investigated by immunohistochemistry. Also small and large intestines were dual-labelled with antibody for VR1 and marker for interstitial cells of Cajal (c-kit). VR1-immunopositive cells were localized on fine fibers in myenteric plexus and expressed weakly myenteric ganglia. The majority of VR1-immunopositive fibers are not colocalized with or apposed to c-kit positive interstitial cells of Cajal. Also electrophysiologically capsaicin had no effect on cultured interstitial cells of Cajal. It is concluded that VR1-immunoreactive intestinal nerves are mainly distributed in myenteric plexus of murine intestinal wall, and vanillod may be not directly related to interstitial cells of Cajal in regulation of intestinal motility.
Animals ; Capsaicin ; Ganglia ; Gastrointestinal Motility ; Immunohistochemistry ; Interstitial Cells of Cajal ; Intestines ; Mice* ; Myenteric Plexus* ; Neurons, Afferent ; Nodose Ganglion ; Spinal Nerve Roots